Abstract:
:Recombinants of Moloney murine leukemia virus (MoMuLV) with either an amphotropic (MoAmphoV) or 10A1-tropic host range (Mo10A1V) induce a spongiform neurodegenerative disease in susceptible mice. To test whether MoMuLV -derived sequences are required for induction of neuropathology, mice were inoculated with either the original 10A1 or the amphotropic (4070A) MuLV isolate. Strikingly, wild-type 10A1 was more neurovirulent than Mo10A1V, inducing severe neurological clinical symptoms with a median latency of 99 days in 100% of infected mice. In contrast, no motor disturbances were detected in any of the 4070A-infected mice, although limited central nervous system lesions were observed. A viral determinant conferring high neurovirulence to 10A1 was mapped to a region encompassing the first 676 bases of the viral genome, including the U5 LTR and encoding the amino-terminus of glycosylated Gag (glycoGag). In contrast to studies with the highly neurovirulent CasFr(KP) virus, an inverse correlation between surface expression levels of glycoGag and neurovirulence was not observed; however, this does not rule out a common underlying mechanism regulating virus pathogenicity.
journal_name
Virologyjournal_title
Virologyauthors
Münk C,Prassolov V,Rodenburg M,Kalinin V,Löhler J,Stocking Cdoi
10.1016/s0042-6822(03)00210-1subject
Has Abstractpub_date
2003-08-15 00:00:00pages
44-55issue
1eissn
0042-6822issn
1096-0341pii
S0042682203002101journal_volume
313pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The phosphatidylinositol-3-kinase (PI3K)-Akt signaling pathway regulates several key cellular functions including protein synthesis, cell growth, glucose metabolism, and inflammation. Many viruses have evolved mechanisms to manipulate this signaling pathway to ensure successful virus replication. The human herpesvirus...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2015.02.040
更新日期:2015-05-01 00:00:00
abstract::The mechanism of intracellular retention for the large surface protein (L) of duck hepatitis B virus (DHBV) was analyzed by examination of the transmembrane topologies and secretory properties of a collection of DHBV L mutants and compared with that of human hepatitis B virus (HBV) L. Our results demonstrate that, in ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.9015
更新日期:1998-03-15 00:00:00
abstract::The HPV16 E7 oncoprotein and 17β-estradiol are important factors for the induction of premalignant lesions and cervical cancer. The study of these factors is crucial for a better understanding of cervical tumorigenesis. Here, we assessed the global gene expression profiles induced by the HPV16 E7 oncoprotein and/or 17...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.08.036
更新日期:2013-12-01 00:00:00
abstract::This report presents a procedure for evaluating the relative affinities of simple sialoside receptor determinants for their interaction with the receptor binding pocket of the influenza virus hemagglutinin. The virus examined was A/Memphis/102/72, previously shown to exhibit preferential binding specificity for enzyma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90026-2
更新日期:1987-10-01 00:00:00
abstract::Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.027
更新日期:2009-10-25 00:00:00
abstract::Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases. The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.036
更新日期:2009-10-10 00:00:00
abstract::Progressive Multifocal Leukoencephalopathy (PML) is caused by lytic replication of JC virus (JCV) in specific cells of the central nervous system. Like other polyomaviruses, JCV encodes a large T-antigen helicase needed for replication of the viral DNA. Here, we report the development of a luciferase-based, quantitati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.07.042
更新日期:2014-11-01 00:00:00
abstract::We have characterized the fibergenes of adenovirus type 19p (Ad19p), Ad19a, and Ad37 by sequencing. The fiber genes of Ad19a and Ad37 are identical and only five amino acids differ comparing Ad19a/Ad37 with Ad19p. Based on the translated sequences we calculated the isoelectrical points (Ips) and found that the fiber k...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8269
更新日期:1997-01-06 00:00:00
abstract::A series of nucleotide substitutions (G46----C; C47----A; C315----U; U317----C) were introduced into the virulence modulating region of the intermediate strain of potato spindle tuber viroid (PSTVd) in order to examine their effect upon viroid infectivity and pathogenicity with the presence of all four mutations resul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90468-5
更新日期:1992-04-01 00:00:00
abstract::Human infections with highly pathogenic H5N1 avian influenza viruses continue to occur in many parts of the world and pose a considerable public health threat. With the use of animal models, the identification of virulence determinants has been instrumental in improving our understanding of how these viruses cause sev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.02.005
更新日期:2011-05-10 00:00:00
abstract::The incidence of head and neck squamous cell carcinomas (HNSCCs) is rising in developed countries. This is driven by an increase in HNSCCs caused by high-risk human papillomavirus (HPV) infections or HPV + HNSCCs. Compared to HNSCCs not caused by HPV (HPV- HNSCCs), HPV + HNSCCs are more responsive to therapy and assoc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.07.004
更新日期:2020-09-01 00:00:00
abstract::Two minor proteins with molecular masses of 8.5 and 15.5 kDa were identified in feline calicivirus (FCV) virions. Direct sequence analysis showed that the N-terminal sequence of the 8.5-kDa protein was identical to that of the predicted protein encoded by open reading frame 3 (ORF3) of the FCV genome. The N-terminal s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0579
更新日期:2000-11-10 00:00:00
abstract::The VP6 gene of human group C rotavirus was cloned and sequenced. Hybridization to the human group C and the porcine group C/Cowden dsRNA genomes assigned this coding sequence to segment 5. The complete human VP6 sequence contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Mr 44,669 Da). The ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90452-h
更新日期:1991-10-01 00:00:00
abstract::A critical feature of highly pathogenic avian influenza viruses (H5N1 and H7N7) is the efficient intracellular cleavage of the hemagglutinin (HA) protein. H7N7 viruses also exist in equine species, and a unique feature of the equine H7N7 HA is the presence of an eleven amino acid insertion directly N-terminal to a tet...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.004
更新日期:2012-12-05 00:00:00
abstract::Influenza antiviral drugs that use protein inhibitors can lose their efficacy as resistant strains emerge. As an alternative strategy, we investigated the use of small interfering RNA molecules (siRNAs) by characterizing three siRNAs (M747, M776 and M832) targeting the influenza matrix 2 gene and three (NS570, NS595 a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.019
更新日期:2016-10-01 00:00:00
abstract::Studies attempting to identify reservoirs of HIV-1 latency have documented that the virus persists as both a latent and productive infection in subsets of CD4(+) cells. Reports regarding establishment of a stable HIV-1 infection in quiescent T cells in vitro, however, are controversial. In the present study, we invest...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.01.014
更新日期:2004-04-10 00:00:00
abstract::Alpha human papillomaviruses (HPVs) are among the most common sexually transmitted agents of which a subset causes cervical neoplasia and cancer in humans. Alpha-PVs have also been identified in non-human primates although few studies have systematically characterized such types. We cloned and characterized 10 distinc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.012
更新日期:2009-10-25 00:00:00
abstract::We previously analyzed hybrids of Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV) that contained CMV RNA2 with the 3'-terminal sequence from TAV RNA2. In this article, we scrutinized the RNA3 molecules in these hybrid viruses by Northern hybridization and RT-PCR and found some recombinant CMV RNA3 molecules...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00050-8
更新日期:2003-02-01 00:00:00
abstract::In HSV types 1 and 2, gH forms a noncovalent heterodimer with gL. Previous studies demonstrated that the first 323 amino acids of gH1 and the first 161 amino acids of gL1 are sufficient for gH/gL binding. For gL1, substitution of any of its four cysteine (C) residues (all located within the gH/gL binding region) destr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.12.006
更新日期:2005-02-20 00:00:00
abstract::Hantaviruses are enveloped RNA viruses that belong to the family Bunyaviridae. They are the causative agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Hantaviruses show a worldwide distribution with specific rodent species as natural hosts. It is known that rodents can tr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.09.006
更新日期:2004-01-05 00:00:00
abstract::Antigenic variation among influenza B viruses is different from that of influenza A in several ways. Antigenic shift has not been observed, distinct antigenic variants of influenza B cocirculate, and antigenically similar viruses have been isolated many years apart. To study the mechanism of antigenic drift in influen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90384-2
更新日期:1984-12-01 00:00:00
abstract::Tombusviruses replicate on pre-existing organelles such as peroxisomes or mitochondria, the membranes of which become extensively reorganized into multivesicular bodies (MVBs) during the infection process. Cucumber necrosis virus (CNV) has previously been shown to replicate in association with peroxisomes in yeast. We...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.12.035
更新日期:2014-03-01 00:00:00
abstract::Genome-length cDNA clones of turnip crinkle virus (TCV) were constructed with SmaI and XbaI restriction sites engineered at the 5' and 3' termini, respectively. The genome-length cDNAs were positioned downstream of modified lambda and T7 phage promoters such that in vitro transcription resulted in RNAs with 5 extra nu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90368-1
更新日期:1989-05-01 00:00:00
abstract::G1/S transition of the cell cycle is blocked when cells are cultured without cell adhesion, even if nutrients and serum are present. Shift experiments involving transfer from adhesion to suspension cultures have revealed that cell adhesion in the early half of G1 phase is required for induction of DNA synthesis. We fo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0591
更新日期:1996-11-01 00:00:00
abstract::Abnormal reinitiation of replication from lambda origins has previously been found during infection in the presence of caffeine or cis-diamminedichloroplatinum II (cis-Pt) or when lambda infects a P2 lysogen. It was further shown that the reinitiations arising from cis-Pt treatment took place during the SOS response i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90201-7
更新日期:1987-06-01 00:00:00
abstract::The surface of the mature dengue virus (DENV) particle consists of 90 envelope (E) protein dimers that mediate both receptor binding and fusion. The E protein ectodomain can be divided into three structural domains designated ED1, ED2, and ED3, of which ED3 contains the critical and dominant virus-specific neutralizat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.05.042
更新日期:2007-09-30 00:00:00
abstract::Ovine pulmonary carcinoma, a contagious lung cancer of sheep, is caused by the oncogenic jaagsiekte sheep retrovirus (JSRV) that is closely related to a family of endogenous sheep retroviral sequences (ESRVs). By using exogenous virus-specific U3 oligonucleotide primers, the entire JSRV proviral genome or its 3' part ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9728
更新日期:1999-06-05 00:00:00
abstract::Successful HIV-1 infection requires a number of specific stages leading to integration of the provirus. We previously suggested that members of the VPAC neuroendocrine receptor family may play a role in HIV-1 infection. We now show that stimulation of the VPAC2 receptor with specific agonists provides strong resistanc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.12.012
更新日期:2007-05-25 00:00:00
abstract::The 5' end of the subgenomic RNA of rubella virus was determined by primer extension. The Maxam-Gilbert sequence ladder of the primer extension product contained a determinable sequence which was colinear with the complement of the sequence of the genomic RNA through nucleotide 3325 from the 3' end of the genomic RNA ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90422-4
更新日期:1989-01-01 00:00:00
abstract::The influenza A virus nucleoprotein previously expressed in Escherichia coli after fusion to 32 heterologous amino acids has now been purified and tested for its ability to form complexes with RNA in vitro. By using a simple filter binding assay, we show that ribonucleoprotein (RNP) complexes form readily with single-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90419-3
更新日期:1987-02-01 00:00:00