Abstract:
:Influenza antiviral drugs that use protein inhibitors can lose their efficacy as resistant strains emerge. As an alternative strategy, we investigated the use of small interfering RNA molecules (siRNAs) by characterizing three siRNAs (M747, M776 and M832) targeting the influenza matrix 2 gene and three (NS570, NS595 and NS615) targeting the nonstructural protein 1 and 2 genes. We also re-examined two previously reported siRNAs, M331 and M950, which target the matrix 1 and 2 genes. Treatment with M331-, M776-, M832-, and M950-siRNAs attenuated influenza titer. M776-siRNA treated cells had 29.8% less infectious virus than cells treated with the previously characterized siRNA, M950. NS570-, NS595- and NS615-siRNAs reduced nonstructural protein 1 and 2 expression and enhanced type I interferon expression by 50%. Combination siRNA treatment attenuated 20.9% more infectious virus than single siRNA treatment. Our results suggest a potential use for these siRNAs as an effective anti-influenza virus therapy.
journal_name
Virologyjournal_title
Virologyauthors
McMillen CM,Beezhold DH,Blachere FM,Othumpangat S,Kashon ML,Noti JDdoi
10.1016/j.virol.2016.07.019subject
Has Abstractpub_date
2016-10-01 00:00:00pages
171-184eissn
0042-6822issn
1096-0341pii
S0042-6822(16)30188-Xjournal_volume
497pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.005
更新日期:2010-03-15 00:00:00
abstract::An SH3-binding domain within the Nef protein of primate lentiviruses has been reported to be important to viral replication and infectivity and dispensable for CD4 downregulation, but its precise role remains unclear. This study investigates the effects of mutations in both the polyproline helix and in the hydrophobic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9897
更新日期:1999-09-15 00:00:00
abstract::It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90239-1
更新日期:1980-10-15 00:00:00
abstract::The nucleoprotein (NP) of the influenza A virus encapsidates the viral RNA and participates in the infectious life cycle of the virus. The aims of this study were to find the degree of conservation of NP among all virus subtypes and hosts and to identify conserved binding sites, which may be utilised as potential drug...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.01.023
更新日期:2014-04-01 00:00:00
abstract::In order to define factors involved in very late Autographa californica nucleopolyhedrovirus (AcMNPV) gene function, random mutagenesis of a baculovirus recombinant (AcUW1.lacZ) by 5'-bromodeoxyuridine treatment was performed. Five viruses were selected with deficiencies in very late gene expression. These were charac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0121
更新日期:1996-03-01 00:00:00
abstract::Prior genetic analysis suggests that there may exist an interaction between the products of the vaccinia virus genes A18R, a putative negative transcription elongation factor, and G2R, a putative positive transcription elongation factor. In addition, affinity purification of polyhistidine-tagged G2R protein overexpres...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9166
更新日期:1998-06-05 00:00:00
abstract::We have mapped the genome of lettuce necrotic yellows virus (LNYV), the type member of the genus cytorhabdovirus of the family Rhabdoviridae. We have cloned and sequenced all intergenic regions and the 3' leader and 5' trailer of the negative-sense, single-stranded RNA genome of LNYV. The LNYV genome appears to contai...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1204
更新日期:1994-05-01 00:00:00
abstract::Hepatitis C virus (HCV) is the major causative agent of blood-borne non-A non-B hepatitis. The persistence of HCV infection is believed to reflect escape from the host immunosurveillance system by mutations in hypervariable region 1 (HVR1) of the envelope protein 2 (E2). Two envelope proteins of HCV, E1 and E2, have b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8516
更新日期:1997-04-28 00:00:00
abstract::Cells transformed with specific regions of the foot-and-mouth disease virus (FMDV) genome have been constructed and analyzed with respect to viability and susceptibility to FMDV infection. Constitutive expression of an active protease 3C under the control of the tk promoter has been documented by the ability of transf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1459
更新日期:1995-09-10 00:00:00
abstract::Neutralizing antibodies (Nabs) are thought to play an important role in prevention and control of HIV-1 infection and should be targeted by an AIDS vaccine. It is critical to understand how HIV-1 induces Nabs by analyzing viral sequences in both tested viruses and sera. Neutralization susceptibility to antibodies in a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.09.031
更新日期:2011-01-20 00:00:00
abstract::Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.027
更新日期:2009-10-25 00:00:00
abstract::We have previously demonstrated that the bZIP transcription factor CREB-2, also called ATF-4, trans-activates, in association with the viral protein Tax, the human T-cell leukemia virus type I (HTLV-I) promoter. In this study, we have examined whether CREB-2 acetylation affects transcriptional activation mediated by T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1501
更新日期:2002-08-01 00:00:00
abstract::The function of rice dwarf virus segment 11 and the corresponding segments of other phytoreoviruses is not yet determined. The amino acid sequence of Pns11, encoded by segment 11, contains a putative zinc finger and five flanking basic regions at the C-terminus. The full-length Pns11 protein and three truncated deriva...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8945
更新日期:1998-01-20 00:00:00
abstract::Tick-borne flaviviruses are maintained in nature in an enzootic cycle involving a tick vector and a vertebrate host. Thus, the virus replicates in two disparate hosts, each providing selective pressures that can influence virus replication and pathogenicity. To identify viral determinants associated with replication i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.030
更新日期:2008-11-25 00:00:00
abstract::Long-term non-progressive HIV infection, characterized by low but detectable viral load and stable CD4 counts in the absence of antiviral therapy, is observed in about 5% of HIV-infected patients. Here we identified four therapy naïve individuals who are strongly seropositive for HIV-1 but who lack evidence of detecta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.06.001
更新日期:2005-08-15 00:00:00
abstract::Recently, cases of severe respiratory illness in military and civilian populations have been associated with a new genomic variant of adenovirus (Ad) serotype 14, designated Ad14a. Compared to the Ad14 reference strain (de Wit), this new virus had a deletion of two amino acid residues in the fiber protein knob. Here w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.02.034
更新日期:2009-05-10 00:00:00
abstract::The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90380-6
更新日期:1986-07-15 00:00:00
abstract::Herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. Evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein C (gC) and one independent of that glycoprotein. Thus, whereas th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90396-9
更新日期:1990-09-01 00:00:00
abstract::The Nef protein of Simian immunodeficiency virus (SIV) associates with multiple T lymphocyte signaling proteins, including the T cell receptor (TCR) zeta chain. We demonstrate here that these interactions are conserved and highly specific. Nefs derived from genetically diverse strains of SIV (SIV(mac)239, SIV(smm)PBj,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1628
更新日期:2002-10-10 00:00:00
abstract::Mammalian type C retroviruses can be detected by their induction of foci of cell transformation in S+I - cells. We have noted that certain subtypes of the mouse xenotropic type C retrovirus (MuLV) inhibit this cell alteration. This inhibition, associated with intact virions, is irreversible and gives the infected cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90044-4
更新日期:1987-09-01 00:00:00
abstract::Fowlpox virus (FPV), type species of the Avipoxvirus genus, causes a slow-spreading pox disease of chickens. Following infection of mammalian cells there is no evidence of productive replication of FPV although cytopathic effects are induced and FPV recombinants have been shown to express foreign genes from vaccinia v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1608
更新日期:1993-11-01 00:00:00
abstract::Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are local...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.12.012
更新日期:2014-02-01 00:00:00
abstract::The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0368
更新日期:1996-07-01 00:00:00
abstract::The 682-nt satellite DNA (sat-DNA) of Tomato leaf curl virus (TLCV) depends on the helper virus for its replication. In contrast to the strict specificity that exists in each geminivirus for its cognate replication associated protein (Rep), TLCV sat-DNA can utilize Rep encoded by distinct geminiviruses. We have used a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1671
更新日期:2003-01-20 00:00:00
abstract::The incidence of head and neck squamous cell carcinomas (HNSCCs) is rising in developed countries. This is driven by an increase in HNSCCs caused by high-risk human papillomavirus (HPV) infections or HPV + HNSCCs. Compared to HNSCCs not caused by HPV (HPV- HNSCCs), HPV + HNSCCs are more responsive to therapy and assoc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.07.004
更新日期:2020-09-01 00:00:00
abstract::The pathogenesis of two Californian strains of myxoma virus (MSW and MSD) was examined in European rabbits (Oryctolagus cuniculus) that were either susceptible to myxomatosis (laboratory rabbits) or had undergone natural selection for genetic resistance to myxomatosis (Australian wild rabbits). MSW was highly lethal f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.007
更新日期:2006-04-25 00:00:00
abstract::The nucleotide sequence of the infectious laryngotracheitis virus (ILTV) gene encoding the 205K complex glycoprotein (gp205) was determined. The gene is contained within a 3-kb EcoRI restriction fragment mapping at approximately map coordinates 0.23 to 0.25 in the UL region of the ILTV genome and is transcribed from r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90859-a
更新日期:1991-09-01 00:00:00
abstract::HIV CRF07 B'/C is a strain circulating mainly in northwest region of China. The gp41 region of CRF07 is derived from a clade C virus. In order to compare the difference of CRF07 gp41 with that of typical clade B virus, we solved the crystal structure of the core region of CRF07 gp41. Compared with clade B gp41, CRF07 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.07.024
更新日期:2013-11-01 00:00:00
abstract::The influenza RNA polymerase is known to catalyse three distinct copying activities: (i) transcription of minus-sense virion RNA (vRNA) into mRNA, (ii) transcription of vRNA into full-length complementary RNA (cRNA), and (iii) transcription of cRNA to vRNA. Ever since the discovery of the conserved 13 and 12 long sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90079-5
更新日期:1992-01-01 00:00:00
abstract::The retroviral integrase (IN) is a virus-encoded enzyme that is essential for insertion of viral DNA into the host chromosome. In order to map and define the properties of a minimal functional domain for this unique viral enzyme, a series of N- and C-terminal deletions of both Rous sarcoma virus (RSV) and human immuno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80060-3
更新日期:1995-01-10 00:00:00