当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Large-scale analysis of influenza A virus nucleoprotein sequence conservation reveals potential drug-target sites.

Large-scale analysis of influenza A virus nucleoprotein sequence conservation reveals potential drug-target sites.

Abstract：

:The nucleoprotein (NP) of the influenza A virus encapsidates the viral RNA and participates in the infectious life cycle of the virus. The aims of this study were to find the degree of conservation of NP among all virus subtypes and hosts and to identify conserved binding sites, which may be utilised as potential drug target sites. The analysis of conservation based on 4430 amino acid sequences identified high conservation in known functional regions as well as novel highly conserved sites. Highly variable clusters identified on the surface of NP may be associated with adaptation to different hosts and avoidance of the host immune defence. Ligand binding potential overlapping with high conservation was found in the tail-loop binding site and near the putative RNA binding region. The results provide the basis for developing antivirals that may be universally effective and have a reduced potential to induce resistance through mutations.

journal_name

Virology

journal_title

Virology

authors

Kukol A,Hughes DJ

doi

10.1016/j.virol.2014.01.023

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

40-7

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(14)00036-1

journal_volume

454-455

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • The ecology of primate retroviruses - an assessment of 12 years of retroviral studies in the Taï national park area, Côte d׳Ivoire.

    abstract：:The existence and genetic make-up of most primate retroviruses was revealed by studies of bushmeat and fecal samples from unhabituated primate communities. For these, detailed data on intra- and within-species contact rates are generally missing, which makes identification of factors influencing transmission a challen...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.05.012

    authors： Gogarten JF,Akoua-Koffi C,Calvignac-Spencer S,Leendertz SA,Weiss S,Couacy-Hymann E,Koné I,Peeters M,Wittig RM,Boesch C,Hahn BH,Leendertz FH

    更新日期：2014-07-01 00:00:00

  • Mutations within the primer binding site of the human immunodeficiency virus type 1 define sequence requirements essential for reverse transcription.

    abstract：:The primer binding site (PBS) is involved in two stages during the reverse transcription of the retroviral RNA genome. In the early stage, the PBS provides complementary sequences through which tRNA(Lys,3) binds the viral RNA genome to initiate minus-strand DNA synthesis; in the later stages, complementarity between t...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0317

    authors： Wakefield JK,Morrow CD

    更新日期：1996-06-15 00:00:00

  • Use of chimeric nectin-1(HveC)-related receptors to demonstrate that ability to bind alphaherpesvirus gD is not necessarily sufficient for viral entry.

    abstract：:Human nectin-1 (HveC, Prr1), a member of the immunoglobulin superfamily and a receptor for the entry of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1), binds to viral gD. For HSV-1, HSV-2, and PRV, the gD-binding region of nectin-1 has been localized to the N-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.0989

    authors： Geraghty RJ,Fridberg A,Krummenacher C,Cohen GH,Eisenberg RJ,Spear PG

    更新日期：2001-07-05 00:00:00

  • Rapid in vivo induction of HIV-specific CD8+ cytotoxic T lymphocytes by a 15-amino acid unmodified free peptide from the immunodominant V3-loop of GP120.

    abstract：:Efforts to generate a vaccine to prevent infection by human immunodeficiency virus (HIV) have focused on inducing neutralizing antibodies. However, cytotoxic T-lymphocyte (CTL) responses are a major immune defense mechanism required for recovery from many different virus infections. Since CTL epitopes can be defined b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90504-i

    authors： Sastry KJ,Nehete PN,Venkatnarayanan S,Morkowski J,Platsoucas CD,Arlinghaus RB

    更新日期：1992-06-01 00:00:00

  • Foot-and-mouth disease virus (FMDV) with a stable FLAG epitope in the VP1 G-H loop as a new tool for studying FMDV pathogenesis.

    abstract：:Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and g...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.11.001

    authors： Lawrence P,Pacheco JM,Uddowla S,Hollister J,Kotecha A,Fry E,Rieder E

    更新日期：2013-02-05 00:00:00

  • Identification of residues on human receptor DPP4 critical for MERS-CoV binding and entry.

    abstract：:Middle East respiratory syndrome coronavirus (MERS-CoV) infects host cells through binding the receptor binding domain (RBD) on its spike glycoprotein to human receptor dipeptidyl peptidase 4 (hDPP4). Here, we report identification of critical residues on hDPP4 for RBD binding and virus entry through analysis of a pan...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.10.006

    authors： Song W,Wang Y,Wang N,Wang D,Guo J,Fu L,Shi X

    更新日期：2014-12-01 00:00:00

  • Structural basis for the dimerization and substrate recognition specificity of porcine epidemic diarrhea virus 3C-like protease.

    abstract：:Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, has caused significant damage to the Asian and American pork industries. Coronavirus 3C-like protease (3CL(pro)), which is involved in the processing of viral polyproteins for viral replication, is an appealing antiviral drug target. Here,...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.04.018

    authors： Ye G,Deng F,Shen Z,Luo R,Zhao L,Xiao S,Fu ZF,Peng G

    更新日期：2016-07-01 00:00:00

  • Phylogenetic analysis of the N8 neuraminidase gene of influenza A viruses.

    abstract：:Phylogenetic analysis of the N8 neuraminidase (NA) genes from 18 influenza A viruses, representing equine and avian hosts in different geographic locations, revealed three major lineages: (i) currently circulating equine 2 viruses; (ii) avian viruses isolated in the Eurasian region, including A/Equine/Jilin/1/89, a re...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1196

    authors： Saito T,Kawaoka Y,Webster RG

    更新日期：1993-04-01 00:00:00

  • Sequence variability and function of measles virus 3' and 5' ends and intercistronic regions.

    abstract：:Sequences critical for the activity of the measles virus (MV) RNA polymerase in transcription and replication were analyzed using a MV genomic cDNA library containing overlapping clones encompassing the entire MV genome. Clones corresponding to the 3' and 5' ends of the MV genome were identified and sequenced, and the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90564-8

    authors： Crowley JC,Dowling PC,Menonna J,Silverman JI,Schuback D,Cook SD,Blumberg BM

    更新日期：1988-06-01 00:00:00

  • Cytotoxic T lymphocytes directed against MAIDS-associated tumors and cells from mice infected by the LP-BM5 MAIDS defective retrovirus.

    abstract：:The LP-BM5 retrovirus, a complex containing ecotropic helper, recombinant MCF, and defective retroviruses, causes an immunodeficiency-termed mouse AIDS (MAIDS). Many disease features of MAIDS resemble those of AIDS, including terminal B cell lymphomas. Previously we generated from MAIDS-susceptible C57BL/6 mice cytoly...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1189

    authors： Green WR,Crassi KM,Schwarz DA,Green KA

    更新日期：1994-04-01 00:00:00

  • Nucleotide and amino acid sequence analysis of the rotavirus nonstructural RNA-binding protein NS35.

    abstract：:NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1059

    authors： Patton JT,Salter-Cid L,Kalbach A,Mansell EA,Kattoura M

    更新日期：1993-02-01 00:00:00

  • Friend murine leukemia virus and spleen focus-forming virus expression in highly malignant interferon-sensitive and interferon-resistant Friend leukemia cells.

    abstract：:Analysis of expression of the Friend murine leukemia virus (F-MuLV) and of the spleen focus forming virus (SFFV) has been undertaken in highly malignant interferon (IFN)-sensitive (745) and IFN-resistant (3Cl-8) Friend leukemia cells (FLC), serially passaged intraperitoneally in DBA/2 mice. In vivo passaged 745 cells,...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90304-1

    authors： Oppi C,Fiorucci G,Ferrantini M,Battistini A,Belardelli F

    更新日期：1986-04-30 00:00:00

  • Identification and characterization of the repetitive DNA element in the genome of insect iridescent virus type 6.

    abstract：:The genome of the Chilo iridescent virus (CIV) was analyzed for existence of repetitive DNA sequences by DNA-DNA hybridization using a defined and complete gene library of the viral genome (209 kbp) and by heteroduplex mapping. These experiments revealed the presence of repetitive DNA elements in the CIV genome, which...

    journal_title：Virology

    pub_type： 杂志文章

    doi：

    authors： Fischer M,Schnitzler P,Delius H,Darai G

    更新日期：1988-12-01 00:00:00

  • Substantial envelope-specific CD8 T-cell immunity fails to control SIV disease.

    abstract：:It is unknown which HIV proteins to target by vaccination in order to generate the most effective CD8 T-cell immunity. We recently immunized SIV(mac251)-infected pigtail macaques with Gag peptides or a cocktail of peptides spanning all SIV proteins, including SIV Env. High-level SIV Env-specific CD8 T-cell responses w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.11.030

    authors： Peut V,Kent SJ

    更新日期：2009-02-05 00:00:00

  • Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission.

    abstract：:Tandemers 2MG, 2MG3, 3MG and 4MG are derivatives of the potent anti-HIV-1 microbicide candidate griffithsin (GRFT). We compared these compounds anti-HIV-1 activity to GRFT using the viruses CAP206.08 and CAAN5342.A2 that have decreased sensitivity to this lectin. The 2MG and 2MG3 tandemers had similar activity to GRFT...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.01.017

    authors： Alexandre K,Malatji K,Mulaudzi T

    更新日期：2020-05-01 00:00:00

  • In vitro processing of human immunodeficiency virus type 1 Gag virus-like particles.

    abstract：:Human immunodeficiency virus (HIV) Gag proteins are assembled into virus particles and then cleaved by the virion-associated HIV protease. Concomitant with Gag processing, doughnut-like HIV particles (the immature form) are converted to particles containing condensed cores (the mature form). Here we describe the in vi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0370

    authors： Morikawa Y,Shibuya M,Goto T,Sano K

    更新日期：2000-07-05 00:00:00

  • Pirates of the Caudovirales.

    abstract：:Molecular piracy is a biological phenomenon in which one replicon (the pirate) uses the structural proteins encoded by another replicon (the helper) to package its own genome and thus allow its propagation and spread. Such piracy is dependent on a complex web of interactions between the helper and the pirate that occu...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2012.10.028

    authors： Christie GE,Dokland T

    更新日期：2012-12-20 00:00:00

  • Amplification of the chromosome 20q region is associated with expression of HPV-16 E7 in human airway and anogenital epithelial cells.

    abstract：:To study the role of human papillomavirus (HPV) infection in the development of genetic instability, we transduced normal human airway and anogenital epithelial cells with various combinations of HPV-16 E6, E7, and the reverse transcriptase component of telomerase (hTERT). Cell lines generated by co-expression of E7 w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.06.027

    authors： Klingelhutz AJ,Qian Q,Phillips SL,Gourronc FA,Darbro BW,Patil SR

    更新日期：2005-09-30 00:00:00

  • Editing the human cytomegalovirus genome with the CRISPR/Cas9 system.

    abstract：:Human Cytomegalovirus (HCMV) is an opportunistic pathogen that causes substantial disease in neonates and immunocompromised individuals. Reverse genetic analysis of the HCMV genome is a powerful tool to dissect the roles that various viral genes play during infection. However, genetic engineering of HCMV is hampered b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.01.021

    authors： King MW,Munger J

    更新日期：2019-03-01 00:00:00

  • Mason-Pfizer monkey virus Gag proteins interact with the human sumo conjugating enzyme, hUbc9.

    abstract：:Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00348-9

    authors： Weldon RA Jr,Sarkar P,Brown SM,Weldon SK

    更新日期：2003-09-15 00:00:00

  • Uncoating of turnip yellow mosaic virus RNA in vivo.

    abstract：:Following mechanical inoculation of leaves with turnip yellow mosaic virus (TYMV), a significant proportion of the retained inoculum is uncoated within 45 sec, and the process is more or less complete after 2 min. At least 80-90% of the uncoating takes place in the epidermis. The application of virus to the intact lea...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90274-0

    authors： Matthews RE,Witz J

    更新日期：1985-07-30 00:00:00

  • Measles virus persistence in an immortalized murine macrophage cell line.

    abstract：:Persistent infection with the Edmonston strain of measles virus (MV) has been established in IC-21 cells, an immortalized murine macrophage cell line. Persistence was established immediately without syncytia formation or cytopathic effects. MV was expressed in the majority of the cells as evidenced by immunofluorescen...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1047

    authors： Goldman MB,Buckthal DJ,Picciotto S,O'Bryan TA,Goldman JN

    更新日期：1995-02-20 00:00:00

  • Coxsackievirus-mediated hyperglycemia is enhanced by reinfection and this occurs independent of T cells.

    abstract：:The induction of autoimmunity by viruses has been hypothesized to occur by a number of mechanisms. Coxsackievirus B4 (CB4) induces hyperglycemia in SJL mice resembling diabetes in humans. While virus is effectively cleared within 2 weeks, hyperglycemia does not appear until about 8-12 weeks postinfection at a time whe...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00462-8

    authors： Horwitz MS,Ilic A,Fine C,Rodriguez E,Sarvetnick N

    更新日期：2003-09-30 00:00:00

  • The latency-associated nuclear antigen tethers the Kaposi's sarcoma-associated herpesvirus genome to host chromosomes in body cavity-based lymphoma cells.

    abstract：:Viruses that establish latent infection must maintain their DNA in the host nucleus through many cellular generations. Here we identify a novel mechanism by which the gammaherpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) may achieve this persistence in latently infected body cavity-based lymphoma (BCBL) cell...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9999

    authors： Cotter MA 2nd,Robertson ES

    更新日期：1999-11-25 00:00:00

  • Characterization of antigenic determinants in the core antigen of the hepatitis C virus.

    abstract：:Antibodies to the hepatitis C virus (HCV) core protein are present in the majority of patients with chronic HCV infection. To characterize the corresponding determinants, synthetic peptides and various deletion clones of the core gene expressed in Escherichia coli were used to test human anti-core positive sera or rab...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1666

    authors： Goeser T,Müller HM,Ye J,Pfaff E,Theilmann L

    更新日期：1994-12-01 00:00:00

  • High-level expression of the measles virus nucleocapsid protein by using a replication-deficient adenovirus vector: induction of an MHC-1-restricted CTL response and protection in a murine model.

    abstract：:Replication-deficient adenovirus (Ad) vectors provide an efficient technology for direct DNA delivery to cells both in vitro and in vivo. We have inserted the measles virus nucleoprotein (N) gene under the control of the strong constitutive CMV major IE promoter into an Ad type 5 E1- vector to produce the recombinant ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1362

    authors： Fooks AR,Schadeck E,Liebert UG,Dowsett AB,Rima BK,Steward M,Stephenson JR,Wilkinson GW

    更新日期：1995-07-10 00:00:00

  • A nucleotide sequence rearrangement distinguishes two isolates of satellite tobacco ringspot virus RNA.

    abstract：:Several strains of tobacco ringspot virus (TobRV) support the replication and encapsidation of satellite tobacco ringspot virus RNA (STobRV RNA). We have compared the nucleotide sequences of four STobRV RNAs, each initially associated with a different isolate of TobRV. A STobRV RNA from a geranium isolate of TobRV and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90049-3

    authors： Buzayan JM,McNinch JS,Schneider IR,Bruening G

    更新日期：1987-09-01 00:00:00

  • Normal replication of vesicular stomatitis virus without C proteins.

    abstract：:The expression of two small basic proteins (C and C') encoded by a second open reading frame of the New Jersey serotype of vesicular stomatitis virus (VSV) P gene was reported previously (Spiropoulou and Nichol, J. Virol., 67, 3103-3110, 1993). Here we found that the Indiana serotype virus also expressed C and C' prot...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0066

    authors： Kretzschmar E,Peluso R,Schnell MJ,Whitt MA,Rose JK

    更新日期：1996-02-15 00:00:00

  • Equine herpesvirus type 1 pUL56 modulates innate responses of airway epithelial cells.

    abstract：:Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.05.023

    authors： Soboll Hussey G,Ashton LV,Quintana AM,Van de Walle GR,Osterrieder N,Lunn DP

    更新日期：2014-09-01 00:00:00

  • TCR triggering transcriptionally downregulates CCR5 expression on rhesus macaque CD4(+) T-cells with no measurable effect on susceptibility to SIV infection.

    abstract：:Studies using transformed human cell lines suggest that most SIV strains use CCR5 as co-receptor. Our analysis of primary rhesus macaque CD4(+) T-cell clones revealed marked differences in susceptibility to SIV(mac)239 infection. We investigated whether different levels of CCR5 expression account for clonal difference...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.10.005

    authors： Minang JT,Trivett MT,Barsov EV,Del Prete GQ,Trubey CM,Thomas JA,Gorelick RJ,Piatak M Jr,Ott DE,Ohlen C

    更新日期：2011-01-05 00:00:00