An Autographa californica nucleopolyhedrovirus lef-2 mutant: consequences for DNA replication and very late gene expression.

Abstract:

:In order to define factors involved in very late Autographa californica nucleopolyhedrovirus (AcMNPV) gene function, random mutagenesis of a baculovirus recombinant (AcUW1.lacZ) by 5'-bromodeoxyuridine treatment was performed. Five viruses were selected with deficiencies in very late gene expression. These were characterized by complementation analysis. One mutant virus, VLD1, was found to be completely deficient in very late gene function. This virus could be complemented by a helper virus to express the very late genes, suggesting that the mutant virus was defective in an activator of very late gene expression. Further studies revealed that the replication of VLD1 was temporally delayed when compared to wild-type virus. The mutation in VLD1 was mapped to a subfragment of the EcoRI-I region of the AcMNPV genome between 0 and 5 map units. Sequence analysis revealed the presence of point mutations in ORF2 and in lef-2. Further mapping experiments demonstrated that only replacement of the point mutation in lef-2 with a wild-type sequence could restore VLD1 to a normal phenotype. Previous studies have suggested that the lef-2 gene product is involved in DNA replication. This was investigated by comparison of DNA replication in wild-type- and VLD1-infected cells. It was found that the mutation in the lef-2 gene of VLD1 did not have an effect on DNA replication. It is proposed that lef-2 may play a dual role, both in DNA replication and very late gene expression.

journal_name

Virology

journal_title

Virology

authors

Merrington CL,Kitts PA,King LA,Possee RD

doi

10.1006/viro.1996.0121

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

338-48

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(96)90121-X

journal_volume

217

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Begomovirus genetic diversity in the native plant reservoir Solanum nigrum: Evidence for the presence of a new virus species of recombinant nature.

    abstract::We examined the native plant host Solanum nigrum as reservoir of genetic diversity of begomoviruses that cause the tomato yellow leaf curl disease (TYLCD) emerging in southern Spain. Presence of isolates of all the species and strains found associated with TYLCD in this area was demonstrated. Mixed infections were com...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2006.02.028

    authors: García-Andrés S,Monci F,Navas-Castillo J,Moriones E

    更新日期:2006-07-05 00:00:00

  • Virological and molecular characterization of a simian human immunodeficiency virus (SHIV) encoding the envelope and reverse transcriptase genes from HIV-1.

    abstract::Simian-human immunodeficiency virus encoding both reverse transcriptase (RT) and envelope genes of HIV-1 (RT Env SHIV) is important for evaluating biomedical prevention modalities for HIV/AIDS. We describe virological characterization of a clade B RT Env SHIV following infection of macaques via multiple routes. In viv...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2012.05.034

    authors: Pal R,Galmin L,Pereira LE,Li B,Zhang J,Li D,Francis J,McNicholl JM,Weiss DE,Smith JM

    更新日期:2012-10-10 00:00:00

  • Transcriptional activity of human papillomavirus type 31b enhancer is regulated through synergistic interaction of AP1 with two novel cellular factors.

    abstract::Transcription of human papillomaviruses (HPV) is regulated by enhancer sequences located in the upstream regulatory region. The factors regulating expression of one of the high risk genital HPV types, HPV 31b, were investigated using transient expression and protein binding assays. A region of 262 base pairs in length...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1390

    authors: Kyo S,Tam A,Laimins LA

    更新日期:1995-08-01 00:00:00

  • Physical characterization of the genome of feline herpesvirus-1.

    abstract::The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(86)90439-3

    authors: Rota PA,Maes RK,Ruyechan WT

    更新日期:1986-10-15 00:00:00

  • Does human papillomavirus-negative condylomata exist?

    abstract::Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deep...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2015.07.023

    authors: Arroyo Mühr LS,Bzhalava D,Lagheden C,Eklund C,Johansson H,Forslund O,Dillner J,Hultin E

    更新日期:2015-11-01 00:00:00

  • Influence of ORF2 on host cell tropism of feline immunodeficiency virus.

    abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0002

    authors: Waters AK,De Parseval AP,Lerner DL,Neil JC,Thompson FJ,Elder JH

    更新日期:1996-01-01 00:00:00

  • Characterization of human papillomavirus-11 mRNAs expressed in the context of autonomously replicating viral genomes.

    abstract::We have previously adapted an experimental system which supports autonomous replication of human papillomavirus-11 (HPV-11) genomic DNA in a human squamous carcinoma cell line (SCC-4) following electroporation of linearized viral DNA. This system allows evaluation of HPV-11 transcription in the context of replicating ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0298

    authors: Renaud KJ,Cowsert LM

    更新日期:1996-06-01 00:00:00

  • Sulfated polymers inhibit the interaction of human cytomegalovirus with cell surface heparan sulfate.

    abstract::Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, heparin) and copolymers of acrylic acid with vinylalcohol sulfate have proved to be potent inhibitors of human cytomegalovirus (CMV) infectivity in vitro. Sulfated alpha-cyclodextrins are only weak inhibitors of CMV. A close correlation was found...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(92)90680-n

    authors: Neyts J,Snoeck R,Schols D,Balzarini J,Esko JD,Van Schepdael A,De Clercq E

    更新日期:1992-07-01 00:00:00

  • Studies on the glycosylation of flavivirus E proteins and the role of carbohydrate in antigenic structure.

    abstract::The glycosylation pattern of several flavivirus E proteins as well as the role of carbohydrate in biological functions and the antigenic structure of tick-borne encephalitis (TBE) virus were investigated by the use of specific endoglycosidases. Endoglycosidase F digestion revealed the presence of a single asparagine-l...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(87)90460-0

    authors: Winkler G,Heinz FX,Kunz C

    更新日期:1987-08-01 00:00:00

  • Bayesian phylodynamic analysis reveals the dispersal patterns of tobacco mosaic virus in China.

    abstract::Tobacco mosaic virus (TMV) is widespread in China and causes considerable economic losses to tobacco production. The molecular epidemiology of this virus is, however, poorly understood. In this study, we sequenced the genomes of 51 TMV isolates from five tobacco-producing regions in China and investigated the dispersa...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2018.12.001

    authors: Gao F,Liu X,Du Z,Hou H,Wang X,Wang F,Yang J

    更新日期:2019-02-01 00:00:00

  • The DNA of bacteriophage 643: isolation and properties of the DNA of a bacteriophage infecting lactic Streptococci.

    abstract::The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 X 10(6). The four species obse...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(84)90406-9

    authors: Lyttle DJ,Petersen GB

    更新日期:1984-03-01 00:00:00

  • Genetic analysis of bipartite geminivirus tissue tropism.

    abstract::The bipartite geminiviruses bean golden mosaic virus (BGMV), cabbage leaf curl virus (CabLCV), and tomato golden mosaic virus (TGMV) exhibit differential tissue tropism in Nicotiana benthamiana. In systemically infected leaves, BGMV remains largely confined to vascular-associated cells (phloem-limited), whereas CabLCV...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2001.1205

    authors: Qin Y,Petty IT

    更新日期:2001-12-20 00:00:00

  • Transcriptional regulation of the open reading frame 35 encoded by Kaposi's sarcoma-associated herpesvirus.

    abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is a member of the Gammaherpesvirinae and is causally associated with Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. The KSHV genome encodes over 85 genes; the function of some is entirely unknown. We have characterized the transcriptio...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2007.08.023

    authors: Masa SR,Lando R,Sarid R

    更新日期:2008-02-05 00:00:00

  • Large-scale analysis of influenza A virus nucleoprotein sequence conservation reveals potential drug-target sites.

    abstract::The nucleoprotein (NP) of the influenza A virus encapsidates the viral RNA and participates in the infectious life cycle of the virus. The aims of this study were to find the degree of conservation of NP among all virus subtypes and hosts and to identify conserved binding sites, which may be utilised as potential drug...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2014.01.023

    authors: Kukol A,Hughes DJ

    更新日期:2014-04-01 00:00:00

  • Identification of four distinct neutralizing epitopes on bluetongue virus serotype 10 using neutralizing monoclonal antibodies and neutralization-escape variants.

    abstract::Neutralizing sites on bluetongue virus serotype 10 (BTV-10) were investigated with a panel of seven neutralizing monoclonal antibodies (MAbs). Each MAb was coupled to agarose beads and tested against the other MAbs and a nonneutralizing control MAb in a competitive immueprecipitation assay. In addition, neutralization...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(90)90041-o

    authors: Heidner HW,Rossitto PV,MacLachlan NJ

    更新日期:1990-06-01 00:00:00

  • Different immunological requirements for protection against acute versus persistent Friend retrovirus infections.

    abstract::The propensity of retroviruses to rapidly establish persistent infections poses a formidable problem in vaccination strategies. In the current study, we use a live attenuated vaccine to study protection against acute and persistent Friend virus infections in mice. Adoptive transfers of immune CD8(+) T cells combined w...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2000.0356

    authors: Dittmer U,Hasenkrug KJ

    更新日期:2000-06-20 00:00:00

  • The Potyviridae P1a leader protease contributes to host range specificity.

    abstract::The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the viral polyprotein, and is closely related to potyviral P1 protease. For its proteolytic activity, P1a requires a still unknown ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2014.12.013

    authors: Shan H,Pasin F,Valli A,Castillo C,Rajulu C,Carbonell A,Simón-Mateo C,García JA,Rodamilans B

    更新日期:2015-02-01 00:00:00

  • Sequence comparison of JSRV with endogenous proviruses: envelope genotypes and a novel ORF with similarity to a G-protein-coupled receptor.

    abstract::Ovine pulmonary carcinoma, a contagious lung cancer of sheep, is caused by the oncogenic jaagsiekte sheep retrovirus (JSRV) that is closely related to a family of endogenous sheep retroviral sequences (ESRVs). By using exogenous virus-specific U3 oligonucleotide primers, the entire JSRV proviral genome or its 3' part ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1999.9728

    authors: Bai J,Bishop JV,Carlson JO,DeMartini JC

    更新日期:1999-06-05 00:00:00

  • Limited susceptibility of mice to Usutu virus (USUV) infection and induction of flavivirus cross-protective immunity.

    abstract::Flaviviruses are RNA viruses that constitute a worrisome threat to global human and animal health. In Europe, West Nile virus (WNV) outbreaks have dramatically increased in number and severity in recent years, with dozens of human and horse deaths and a high avian mortality across the continent. Besides WNV, the only ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2015.03.020

    authors: Blázquez AB,Escribano-Romero E,Martín-Acebes MA,Petrovic T,Saiz JC

    更新日期:2015-08-01 00:00:00

  • Determination of a splice acceptor site of pX gene in HTLV-I infected cells.

    abstract::The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine e...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(85)90404-0

    authors: Okamoto T,Josephs SF,Kawanishi M,Wong-Staal F

    更新日期:1985-06-01 00:00:00

  • The effect of defective interfering RNAs on the accumulation of tomato bushy stunt virus proteins and implications for disease attenuation.

    abstract::Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNA...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1410

    authors: Scholthof KB,Scholthof HB,Jackson AO

    更新日期:1995-08-01 00:00:00

  • Potential length determiner and DNA injection protein is extruded from bacteriophage T4 tail tubes in vitro.

    abstract::Bacteriophage T4 tails contain a set of extended protein molecules in the central channel of the tail tube through which the DNA must exit during infection. Treatment of tails with guanidine hydrochloride separates the baseplates, leaving the tail tube and several specific tube-associated proteins. Methods were develo...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(86)90051-6

    authors: Duda RL,Gingery M,Eiserling FA

    更新日期:1986-06-01 00:00:00

  • Localization of multiple TMV encapsidation initiation sites on rbcL gene transcripts.

    abstract::TMV capsid protein reacts with and encapsidates many of the chloroplast DNA transcripts both in vivo and in vitro to form pseudovirions. We report on the encapsidation initiation reaction with one of the major RNA species found in in vivo formed pseudovirions, the mRNA for the chloroplast-encoded large subunit of ribu...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(89)90280-8

    authors: Atreya CD,Siegel A

    更新日期:1989-02-01 00:00:00

  • A cis-replication element functions in both orientations to enhance replication of Turnip crinkle virus.

    abstract::Turnip crinkle virus (TCV) (family Tombusviridae, genus Carmovirus) is a positive-sense RNA virus containing a 4054-base genome. Previous results indicated that insertion of Hairpin 4 (H4) into a TCV-associated satellite RNA enhanced replication 6-fold in vivo (Nagy, P., Pogany, J., Simon, A. E., 1999. EMBO J. 18:5653...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2006.03.051

    authors: Sun X,Simon AE

    更新日期:2006-08-15 00:00:00

  • A comparison of viruses infecting two different Chlorella-like green algae.

    abstract::Five plaque-forming viruses (Pbi viruses) of the unicellular, eukaryotic, exsymbiotic Chlorella-like green alga strain Pbi were isolated from fresh water collected in Germany. The viruses were compared to two previously characterized plaque-forming viruses (NC64A viruses) of Chlorella strain NC64A. The Pbi viruses do ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(88)90063-3

    authors: Reisser W,Burbank DE,Meints SM,Meints RH,Becker B,Van Etten JL

    更新日期:1988-11-01 00:00:00

  • Genetic characterization of an adapted pandemic 2009 H1N1 influenza virus that reveals improved replication rates in human lung epithelial cells.

    abstract::The 2009 influenza pandemic originated from a swine-origin H1N1 virus, which, although less pathogenic than anticipated, may acquire additional virulence-associated mutations in the future. To estimate the potential risk, we sequentially passaged the isolate A/Hamburg/04/2009 in A549 human lung epithelial cells. After...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2016.02.002

    authors: Wörmann X,Lesch M,Welke RW,Okonechnikov K,Abdurishid M,Sieben C,Geissner A,Brinkmann V,Kastner M,Karner A,Zhu R,Hinterdorfer P,Anish C,Seeberger PH,Herrmann A,Meyer TF,Karlas A

    更新日期:2016-05-01 00:00:00

  • Conserved aromatic residues of the hepatitis B virus Precore propeptide are involved in a switch between distinct dimeric conformations and essential in the formation of heterocapsids.

    abstract::The Hepatitis B virus Precore protein, present in the secretory pathway as the HBeAg precursor, can associate in the cytoplasm with the Core protein to form heterocapsids, likely to favor viral persistence. Core and Precore proteins share their primary sequence except for ten additional aminoacids at the N-terminus of...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2014.06.013

    authors: Duriez M,Thouard A,Bressanelli S,Rossignol JM,Sitterlin D

    更新日期:2014-08-01 00:00:00

  • Adenovirus transformation revertant resistant to retransformation by E1 but not by SV40-T and HPV16-E7 oncogenes.

    abstract::We have previously described a revertant cell line which expresses a dominant tumor suppressor phenotype to E1 but not to heterologous oncogenes such as c-myc, N-ras, or polyoma middle t (Sircar et al. (1988) Oncogene 3, 725-728). DNA tumor virus oncogenes have been suggested to transform cells via the common mechanis...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(92)90180-w

    authors: Sircar S,Horvath J,Roberge D,Diouri M,Weber JM

    更新日期:1992-11-01 00:00:00

  • The insertion domain of the duck hepatitis B virus core protein plays a role in nucleocapsid assembly.

    abstract::Synthesis of hepadnaviral DNA is dependent upon both the viral DNA polymerase and the viral core protein, the subunit of the nucleocapsids in which viral DNA synthesis takes place. In a study of natural isolates of duck hepatitis B virus (DHBV), we cloned full-length viral genomes from a puna teal. One of the clones f...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2006.06.004

    authors: Guo H,Aldrich CE,Saputelli J,Xu C,Mason WS

    更新日期:2006-09-30 00:00:00

  • Molecular cloning and mapping of rat cytomegalovirus DNA.

    abstract::The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty KpnI fragments were similarly cloned which, together with the HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragm...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(88)90155-9

    authors: Burns WH,Barbour GM,Sandford GR

    更新日期:1988-09-01 00:00:00