Sulfated polymers inhibit the interaction of human cytomegalovirus with cell surface heparan sulfate.

abstract：:We examined the native plant host Solanum nigrum as reservoir of genetic diversity of begomoviruses that cause the tomato yellow leaf curl disease (TYLCD) emerging in southern Spain. Presence of isolates of all the species and strains found associated with TYLCD in this area was demonstrated. Mixed infections were com...

journal_title：Virology

authors： García-Andrés S,Monci F,Navas-Castillo J,Moriones E

更新日期：2006-07-05 00:00:00

abstract：:Homologous, subgenomic fragments of the viral LCR and E6/E7 transforming genes of HPV-18 and HPV-16 were amplified from several primary cervical, penile, and vulvar tumors and cloned into a pUC-18-derived vector. When assayed by a quantitative transformation assay using primary human keratinocytes, the subgenomic regi...

journal_title：Virology

authors： Villa LL,Schlegel R

更新日期：1991-03-01 00:00:00

abstract：:The oncogenic potential of human papillomavirus type 18 which is found in a significant number of cervical and penile cancer biopsies was tested in primary human keratinocytes derived from neonatal foreskin. Viral DNA and a gene for resistance to neomycin were introduced into these cells by calcium phosphate transfect...

journal_title：Virology

authors： Kaur P,McDougall JK

更新日期：1989-11-01 00:00:00

abstract：:A full-length cDNA clone of the U1 (common) strain of tobacco mosaic virus (TMV) was constructed, and highly infectious transcripts were produced in vitro using bacteriophage T7 RNA polymerase. Frameshift mutations designed to cause premature termination of translation were introduced into either the 30-kDa movement p...

journal_title：Virology

authors： Holt CA,Beachy RN

更新日期：1991-03-01 00:00:00

abstract：:Methods currently used for HIV-1 viral load measurements are very sensitive, but cannot distinguish between infectious and noninfectious particles. Here we describe the development of a novel, sensitive, and highly reproducible method that allows rapid isolation and quantification of infectious particles from patient ...

journal_title：Virology

authors： Rusert P,Fischer M,Joos B,Leemann C,Kuster H,Flepp M,Bonhoeffer S,Günthard HF,Trkola A

更新日期：2004-08-15 00:00:00

abstract：:The mature flavivirus capsid protein (virion C) is commonly thought to be free in the cytoplasm of infected cells and to form a nucleocapsid-like complex with genomic RNA in mature virus particles. There is little sequence conservation among flavivirus virion C proteins, but they are similar in size (e.g., 99 amino ac...

journal_title：Virology

authors： Markoff L,Falgout B,Chang A

更新日期：1997-06-23 00:00:00

abstract：:Activation of the minute virus of mice (MVM) P4 promoter is a key step in the life cycle of the virus and is completely dependent on host transcription factors. Since transcription-factor composition varies widely in different cell types, there is the possibility that only some cell types in the host organism have the...

journal_title：Virology

authors： Davis C,Segev-Amzaleg N,Rotem I,Mincberg M,Amir N,Sivan S,Gitelman I,Tal J

更新日期：2003-02-15 00:00:00

abstract：:We have examined the nuclear and cytoplasmic distribution of the latency-associated transcripts (LATs) of HSV-1. During latency these transcripts accumulate in the nuclei of neurons in the peripheral and central nervous system of infected animals. However, our Northern blot analyses demonstrate that the 2-kb LAT is fo...

journal_title：Virology

authors： Nicosia M,Zabolotny JM,Lirette RP,Fraser NW

更新日期：1994-11-01 00:00:00

abstract：:Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...

journal_title：Virology

authors： Volkman LE,Zaal KJ

更新日期：1990-03-01 00:00:00

abstract：:A non-optimal receptor-binding specificity of avian influenza viruses is believed to hamper their replication in humans; however, the magnitude of this restriction remains undefined. Here we generated recombinant viruses, R1 and R2, that differed solely by two amino acids in the receptor-binding site of their hemagglu...

journal_title：Virology

authors： Matrosovich M,Matrosovich T,Uhlendorff J,Garten W,Klenk HD

更新日期：2007-05-10 00:00:00

abstract：:Unlike cell lines transformed with Polyomaviruses, transformants derived by focus formation or colony formation in agarose medium after transfer into rat fibroblast cells (FR3T3 line) of Bovine Papilloma Type 1 (BPV1) DNA were consistently observed to grow poorly in suspension and to remain highly serum dependent for ...

journal_title：Virology

authors： Grisoni M,Meneguzzi G,de Lapeyrière O,Binétruy B,Rassoulzadegan M,Cuzin F

更新日期：1984-06-01 00:00:00

abstract：:Red sea bream iridovirus (RSIV) of the Iridoviridae family is a causative agent of lethal infections in many cultured marine fish species in southwestern Japan. RSIV-induced apoptosis was divided as follows: (1). cell shrinkage and rounding at the early apoptotic stage, (2). cell enlargement at the middle apoptotic st...

journal_title：Virology

authors： Imajoh M,Sugiura H,Oshima S

更新日期：2004-05-01 00:00:00

abstract：:Published ultraviolet (uv) inactivation data and in vitro transcription studies have suggested that vesicular stomatitis virus (VSV) leader RNA was solely responsible for the inhibition of host cell RNA synthesis by this virus. Since no protein product is encoded in leader RNA, this conclusion implied that no protein ...

journal_title：Virology

authors： Poirot MK,Schnitzlein WM,Reichmann ME

更新日期：1985-01-15 00:00:00

abstract：:Five revertants of a linker-scanning mutation adjacent to the stem-loop V attenuation determinant (X472) in the 5' noncoding region of poliovirus RNA were independently isolated from neuroblastoma cells and contained RNAs with seven nucleotide changes in the pyrimidine-rich region. Generation of the identical rare sec...

journal_title：Virology

authors： Stewart SR,Semler BL

更新日期：1999-11-25 00:00:00

abstract：:The surface of the mature dengue virus (DENV) particle consists of 90 envelope (E) protein dimers that mediate both receptor binding and fusion. The E protein ectodomain can be divided into three structural domains designated ED1, ED2, and ED3, of which ED3 contains the critical and dominant virus-specific neutralizat...

journal_title：Virology

authors： Gromowski GD,Barrett AD

更新日期：2007-09-30 00:00:00

abstract：:In this study, we show how the red spotted grouper nervous necrosis virus (RGNNV) causes loss of mitochondrial membrane potential and promotes host secondary apoptotic necrosis. RGNNV viral proteins such as protein alpha (42 kDa) and protein A (110 kDa) were quickly expressed between 12 h and 24 h postinfection (p.i.)...

journal_title：Virology

authors： Chen SP,Yang HL,Her GM,Lin HY,Jeng MF,Wu JL,Hong JR

更新日期：2006-04-10 00:00:00

abstract：:The location of the RNA start point of in vivo Q-activated late gene RNA of bacteriophage lambda has been determined to be identical to the start point of in vitro 6 S RNA. The 6 S RNA is made early in infection and is efficiently antiterminated by Q. Two RNase III cut sites are located within Q-dependent RNA sequence...

journal_title：Virology

authors： Daniels DL,Subbarao MN,Blattner FR,Lozeron HA

更新日期：1988-12-01 00:00:00

abstract：:The technique of cDNA polymerase chain reaction was used to study the heterogeneity of adenovirus type 12 early region 1A mRNAs in infected and transformed cells. In addition to 13 S and 12 S mRNAs, three transcripts of 10 S, 9.5 S, and 9 S could be isolated from infected HeLa cells. A fourth transcript of 11 S was fo...

journal_title：Virology

authors： Brockmann D,Tries B,Esche H

更新日期：1990-12-01 00:00:00

abstract：:Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases. The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells a...

journal_title：Virology

authors： Shafti-Keramat S,Schellenbacher C,Handisurya A,Christensen N,Reininger B,Brandt S,Kirnbauer R

更新日期：2009-10-10 00:00:00

abstract：:It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...

journal_title：Virology

authors： Stanley J,Goldbach R,Van Kammen A

更新日期：1980-10-15 00:00:00

abstract：:Experiments with monoclonal antibodies raised against two laboratory strains of Sindbis virus, SB and SIN, suggested the existence of a strain-specific neutralizing antigenic site (E2-b) on the E2 glycoprotein. A comparison of monoclonal antibody binding patterns and E2 glycoprotein gene sequences of six laboratory st...

journal_title：Virology

authors： Davis NL,Pence DF,Meyer WJ,Schmaljohn AL,Johnston RE

更新日期：1987-11-01 00:00:00

abstract：:The P150 and P90 replicase proteins of rubella virus (RUBV), a plus-strand RNA Togavirus, produce a unique cytoplasmic fiber network resembling microtubules. Pharmacological and mutagenic approaches were used to determine if these fibers functioned in virus replication. The pharmacological approach revealed that micro...

journal_title：Virology

authors： Matthews JD,Tzeng WP,Frey TK

更新日期：2010-10-25 00:00:00

abstract：:Although v-src, the oncogene of Rous sarcoma virus, has been shown to be capable of inducing lethal tumors at visceral sites distal to the primary tumor mass, the mechanisms opposing visceral tumor formation remain to be elucidated. In the present study, we show that visceral tumors, many of which represent a metastas...

journal_title：Virology

authors： Halpern MS,Wisner TW,Brown IP,England JM

更新日期：1993-11-01 00:00:00

abstract：:The role that interferon-gamma (IFNgamma) plays during herpetic stromal keratitis (HSK) has not been definitively determined. In primary HSK most reports suggest that IFNgamma may help control viral replication and contribute to corneal pathology. However, its role in recurrent HSK has not been directly addressed. The...

journal_title：Virology

authors： Keadle TL,Alexander DE,Leib DA,Stuart PM

更新日期：2008-10-10 00:00:00

abstract：:Nuclear Matrix and MARs have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear. Here, we show that >98% of HIV sequences contain consensus MAR element in their promoter. We show that SMAR1 binds to the LTR MAR and reinforces t...

journal_title：Virology

authors： Sreenath K,Pavithra L,Singh S,Sinha S,Dash PK,Siddappa NB,Ranga U,Mitra D,Chattopadhyay S

更新日期：2010-04-25 00:00:00

abstract：:Bovine herpesvirus 5 (BHV-5) is a neurovirulent alpha-herpesvirus that causes fatal encephalitis in calves. We previously demonstrated that deletion of a glycine-rich epitope in the gE ectodomain dramatically reduced BHV-5 neurovirulence. To investigate the role of gE cytoplasmic tail sequences in the neuropathogenesi...

journal_title：Virology

authors： Al-Mubarak A,Simon J,Coats C,Okemba JD,Burton MD,Chowdhury SI

更新日期：2007-09-01 00:00:00

abstract：:While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...

journal_title：Virology

authors： Cristillo AD,Wang S,Caskey MS,Unangst T,Hocker L,He L,Hudacik L,Whitney S,Keen T,Chou TH,Shen S,Joshi S,Kalyanaraman VS,Nair B,Markham P,Lu S,Pal R

更新日期：2006-03-01 00:00:00

abstract：:The longest hepatitis B virus transcript is a 3.9-kb mRNA whose function remained unclear. In this study, we wished to identify the translation products and physiological role of this viral transcript. This transcript initiates from the X promoter region ignoring the inefficient and noncanonical viral polyadenylation ...

journal_title：Virology

authors： Doitsh G,Shaul Y

更新日期：2003-05-10 00:00:00

abstract：:Spontaneous proteolysis of influenza virus M1 protein during crystallisation has defined an N-terminal domain of amino acids 1--164. Full-length M1, the N-terminal domain, and the C-terminal part of M1 (residues 165--252) were produced in Escherichia coli. In vitro tests showed that only full-length M1 and its N-termi...

journal_title：Virology

authors： Baudin F,Petit I,Weissenhorn W,Ruigrok RW

更新日期：2001-03-01 00:00:00

abstract：:Some satellite (sat-) and defective interfering (DI) RNAs associated with plant viruses intensify or ameliorate the symptoms of the virus. We recently demonstrated that the TCV coat protein (CP) is involved in symptom modulation by sat-RNA C. Two additional subviral RNAs have now been tested for effect of the CP on sy...

journal_title：Virology

authors： Kong Q,Oh JW,Carpenter CD,Simon AE

更新日期：1997-11-24 00:00:00