Abstract:
:Five revertants of a linker-scanning mutation adjacent to the stem-loop V attenuation determinant (X472) in the 5' noncoding region of poliovirus RNA were independently isolated from neuroblastoma cells and contained RNAs with seven nucleotide changes in the pyrimidine-rich region. Generation of the identical rare second-site mutations suggests the existence of a replicase-dependent mutagenesis mechanism during poliovirus replication. Enzymatic structure probing of the mutated pyrimidine-rich domain identified secondary structure changes between stem-loops V and VI. A consensus secondary structure model is presented for wild-type stem-loops V and VI and the pyrimidine-rich region located in the 5' noncoding region of poliovirus RNA. A pyrimidine-rich region mutant (X472-R4N) produced large plaques in neuroblastoma cells and small plaques in HeLa cells, but the plaque size differences were not due to cell-type differences in viral translation or RNA replication. Release of X472-R4N from HeLa cells was 10-fold lower than release from neuroblastoma cells, which may explain the small plaque phenotype of X472-R4N in HeLa cells. Wild-type poliovirus was also released more efficiently from neuroblastoma cells (approximately 4-fold increase compared with release from HeLa cells), indicating that poliovirus neurotropism may be influenced by the cell-type efficiency of virus release. Thermal treatment increased the levels of infectious X472-R4N virions but not wild-type virus particles; thus RNA sequence and structural changes in the mutated 5' noncoding region of X472-R4N may have altered RNA-protein interactions necessary for virus infectivity.
journal_name
Virologyjournal_title
Virologyauthors
Stewart SR,Semler BLdoi
10.1006/viro.1999.9981subject
Has Abstractpub_date
1999-11-25 00:00:00pages
385-97issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(99)99981-6journal_volume
264pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Bipartite geminiviruses are small, plant-infecting viruses with genomes composed of circular, single-stranded DNA molecules, designated A and B. Although they are closely related genetically, individual bipartite geminiviruses frequently exhibit host-specific adaptation. Two such viruses are bean golden mosaic virus (...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0620
更新日期:2000-11-25 00:00:00
abstract::Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90037-8
更新日期:1989-03-01 00:00:00
abstract::We previously analyzed hybrids of Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV) that contained CMV RNA2 with the 3'-terminal sequence from TAV RNA2. In this article, we scrutinized the RNA3 molecules in these hybrid viruses by Northern hybridization and RT-PCR and found some recombinant CMV RNA3 molecules...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00050-8
更新日期:2003-02-01 00:00:00
abstract::The guinea pig cytomegalovirus (GPCMV) co-linear gene and potential functional homolog of HCMV UL84 (GP84) was investigated. The GP84 gene had delayed early transcription kinetics and transient expression studies of GP84 protein (pGP84) demonstrated that it targeted the nucleus and co-localized with the viral DNA poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.10.028
更新日期:2011-02-05 00:00:00
abstract::The yeast retrotransposon, Ty1, produces a macromolecular structure known as a virus-like particle (VLP) as an essential part of its replication cycle. The Ty1 Gag-like structural protein TYA, p1-440, alone is capable of directing assembly of the VLP. In order to determine the TYA sequences required for assembly, we h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1454
更新日期:1995-09-10 00:00:00
abstract::During Herpes simplex virus envelopment, capsids, tegument polypeptides, and membrane proteins assemble at the site of budding and a cellular lipid bilayer becomes refashioned into a spherical envelope. Though the molecular interactions driving these events are poorly understood, several lines of evidence suggest that...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.08.023
更新日期:2003-12-05 00:00:00
abstract::The complete nucleotide sequence of the RSV cpts-248/404 live attenuated vaccine candidate was determined from cloned cDNA and was compared to that of the RSV A2/HEK7 wild-type, cold-passaged cp-RSV, and cpts-248 virus, which constitute the series of progenitor viruses. RSV cpts-248/404 is more attenuated and more tem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0618
更新日期:1996-11-15 00:00:00
abstract::Mammalian type C retroviruses can be detected by their induction of foci of cell transformation in S+I - cells. We have noted that certain subtypes of the mouse xenotropic type C retrovirus (MuLV) inhibit this cell alteration. This inhibition, associated with intact virions, is irreversible and gives the infected cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90044-4
更新日期:1987-09-01 00:00:00
abstract::African swine fever virus (ASFV) growth and plaque formation were inhibited by phosphonoacetic acid (PAA) concentrations of 200 micrograms/ml or more. One spontaneous mutant and two mutants isolated from mutagenized virus were resistant to PAA inhibition and showed practically normal viral DNA synthesis in the presenc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.9953
更新日期:1995-12-01 00:00:00
abstract::Langat (LGT) flavivirus, derived from infectious full-length cDNA clone 636, was investigated for its apoptotic activities in mouse neuroblastoma (Neuro-2a) and simian kidney (Vero and LLC-MK(2)) cells. The hallmark of apoptosis, cleavage of cellular DNA, was observed 48 h after infection of Vero, LLC-MK(2), and Neuro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0980
更新日期:2001-08-01 00:00:00
abstract::The tegument of herpes simplex virus contains proteins important for the efficient initiation of infection. L-particles are noninfectious virion-related particles that lack the nucleocapsid but do contain tegument and envelope. Their ability to deliver functional proteins into cells was compared to that of virions by ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90906-6
更新日期:1992-10-01 00:00:00
abstract::In this study, we show how the red spotted grouper nervous necrosis virus (RGNNV) causes loss of mitochondrial membrane potential and promotes host secondary apoptotic necrosis. RGNNV viral proteins such as protein alpha (42 kDa) and protein A (110 kDa) were quickly expressed between 12 h and 24 h postinfection (p.i.)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.052
更新日期:2006-04-10 00:00:00
abstract::RNase protection analysis of mRNAs for Epstein-Barr virus (EBV) genes activated early in the induction of the productive cycle in Akata cells has confirmed that BZLF1 and BRLF1 behave as immediate early genes. These are likely to be the only virus immediate early genes since analysis of three other transcripts previou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1070
更新日期:1993-02-01 00:00:00
abstract::The monoclonal antibody 2D5 neutralized vaccinia virus by preventing penetration of the virus and reacting with VP23-29K. The conformation of the VP23-29K was maintained by a disulfide bond(s), and the 2D5mAb reacted stronger with the nonreduced 23-kDa form than with the reduced 29-kDa form. We selected several escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0337
更新日期:1996-06-15 00:00:00
abstract::A mutant (Tbts7) of alfalfa mosaic virus, the coat protein of which is unable to activate the viral genome (the RNA species 1, 2, and 3, which need some coat protein for infectivity) at 30 degrees , can be rescued at this temperature by adding to the inoculum wild-type RNA 3 (the genome part that contains the coat pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90528-1
更新日期:1982-02-01 00:00:00
abstract::Five regions on the coat protein of BNYVV which had been shown previously to be involved in the formation of continuous epitopes were further analyzed by means of synthetic overlapping peptides. It was found that at least some of these regions may encompass several overlapping epitopes (or parts thereof). Four monoclo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1031
更新日期:1994-01-01 00:00:00
abstract::While several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed to be monocytes which are poorly infected directly, but are highly susce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.07.010
更新日期:2011-09-15 00:00:00
abstract::Human papillomavirus (HPV) is a family of viruses divided into five genera: alpha, beta, gamma, mu, and nu. There is an ongoing discussion about whether beta genus HPVs (β-HPVs) contribute to cutaneous squamous cell carcinoma (cSCC). The data presented here add to this conversation by determining how a β-HPV E6 protei...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.07.016
更新日期:2020-10-01 00:00:00
abstract::The V3 region of the HIV-1 envelope (Env) glycoprotein gp120 is a key functional domain yet it exhibits distinct mutational patterns across subtypes. Here an invariant residue (Ile 309) was replaced with Leu in 7 subtype C patient-derived Envs from recent infection and 4 related neutralizing antibody escape variants t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.04.010
更新日期:2010-08-15 00:00:00
abstract::A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we dem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.09.028
更新日期:2004-01-05 00:00:00
abstract::Naturally occurring mutations in Human T-cell Leukemia Virus Type 1 (HTLV-1) Tax protein lead to loss of recognition by cytotoxic T-lymphocytes. Most of these mutations also abolish or severely impair the transactivation function of Tax. Ninety percent of the rex gene, which encodes the viral regulator of mRNA splicin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8789
更新日期:1997-10-27 00:00:00
abstract::We have previously adapted an experimental system which supports autonomous replication of human papillomavirus-11 (HPV-11) genomic DNA in a human squamous carcinoma cell line (SCC-4) following electroporation of linearized viral DNA. This system allows evaluation of HPV-11 transcription in the context of replicating ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0298
更新日期:1996-06-01 00:00:00
abstract::The amino-terminal domain of influenza A virus matrix protein (residues 1-164) was crystallized at pH 7 into a new crystal form in space group P1. This packing of the protein implies that M1(1-164) was monomeric in solution when it crystallized. Otherwise, the structure of the M1 fragment in the pH 7 crystals was the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0727
更新日期:2001-01-20 00:00:00
abstract::Open reading frame 89 (ORF89) is one of the three genes that are believed to be involved in the latent infection of white spot syndrome virus (WSSV). Here, we report the structure and functional characterization of ORF89. cDNA sequencing, 5' RLM-RACE, and 3' RLM-RACE showed that ORF89 gene is transcribed into an unspl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.05.005
更新日期:2004-07-20 00:00:00
abstract::Varicella zoster virus (VZV) is a neurotropic alphaherpesvirus that, following primary infection (varicella), establishes latency in sensory, autonomic, sympathetic and parasympathetic neurons, where it remains until reactivation (zoster). VZV-specific cell-mediated immune responses maintain VZV latency; thus, immunos...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.06.013
更新日期:2018-09-01 00:00:00
abstract::New sequences have been obtained by successive overlapping RT-PCR extensions from the pol region of a retroviral RNA (multiple sclerosis-associated retroviral element, MSRV) amplified in retrovirus-like particles from patients with multiple sclerosis. gag and pol sequences are related to type C oncoviruses, whereas th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9792
更新日期:1999-07-20 00:00:00
abstract::The transcription/replication activity of ribonucleoproteins derived from influenza A primary isolates of human (A/Paris/908/97) or avian origin (A/Mallard/Marquenterre/MZ237/83, A/Hong Kong/156/97) was compared upon reconstitution in mammalian or avian cells, using viral-like reporter RNAs synthesized under the contr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.12.027
更新日期:2007-06-05 00:00:00
abstract::Human infections with highly pathogenic H5N1 avian influenza viruses continue to occur in many parts of the world and pose a considerable public health threat. With the use of animal models, the identification of virulence determinants has been instrumental in improving our understanding of how these viruses cause sev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.02.005
更新日期:2011-05-10 00:00:00
abstract::Sodium taurocholate cotransporting polypeptide (NTCP) is a functional receptor for human hepatitis B virus (HBV) and its satellite virus Hepatitis D virus (HDV). Physiologically, NTCP is responsible for the majority of sodium-dependent bile acids uptake by hepatocytes. The p.Ser267Phe (S267F) variant of NTCP is a sing...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.07.006
更新日期:2018-09-01 00:00:00
abstract::We used a mouse nasal model of herpes simplex virus 2 (HSV-2) infection to examine the biological properties of HSV-2 wild-type (wt), TK-negative, and replication-defective strains in vivo. Nasal septa tissue is the major site of wt viral replication post intranasal (i.n.) inoculation. The HSV-2 strain 186 syn(+)-1 wt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0628
更新日期:2000-12-05 00:00:00