Molecular cloning and characterization of MSRV-related sequences associated with retrovirus-like particles.

abstract：:The Human Papillomavirus type-16 (HPV-16) E6 and E7 oncogenes are selectively retained and expressed in cervical carcinomas, and expression of E6 and E7 is sufficient to immortalize human cervical epithelial cells. Expression of the epidermal growth factor receptor (EGFR) is often increased in cervical dysplasia and c...

journal_title：Virology

authors： Woodworth CD,Diefendorf LP,Jette DF,Mohammed A,Moses MA,Searleman SA,Stevens DA,Wilton KM,Mondal S

更新日期：2011-12-05 00:00:00

abstract：:Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...

journal_title：Virology

authors： Sato H,Watanabe S,Furuno A,Yoshiike K

更新日期：1989-05-01 00:00:00

abstract：:We expressed the carboxy-terminal portion of the E2 open reading frame (ORF)-encoded protein of human papillomavirus type 16 (HPV-16) and purified it to near homogeneity. Using DNase I footprinting techniques, we show that like the homologous protein from bovine papillomavirus type 1 (BPV-1), HPV-18, HPV-11, it binds ...

journal_title：Virology

authors： Bedrosian CL,Bastia D

更新日期：1990-02-01 00:00:00

abstract：:The Psu (Polarity suppression) protein of satellite bacteriophage P4 was first characterized as an anti-terminator of transcription termination in Escherichia coli. Psu is also a structural component of mature P4 capsids, where it is present as a decoration protein. Psu is located externally on the capsid surface, and...

journal_title：Virology

authors： Isaksen ML,Dokland T,Lindqvist BH

更新日期：1993-06-01 00:00:00

abstract：:A class of disulfide constrained peptides containing a core motif FPWG was identified from a screen of phage displayed library using the HCV RNA-dependent RNA polymerase (NS5B) as a bait. Surface plasmon resonance studies showed that three highly purified synthetic constrained peptides bound to immobilized NS5B with e...

journal_title：Virology

authors： Amin A,Zaccardi J,Mullen S,Olland S,Orlowski M,Feld B,Labonte P,Mak P

更新日期：2003-08-15 00:00:00

abstract：:The mechanism of vaccinia virus-induced selective inhibition of host cell protein synthesis was studied in a nonpermissive (Chinese hamster ovary, CHO) and in a permissive mouse cell line ( L cells). Small polyadenylated RNAs obtained from uninfected and infected cells were fractionated into six size classes by polyac...

journal_title：Virology

authors： Su MJ,Bablanian R

更新日期：1990-12-01 00:00:00

abstract：:A resonance energy transfer assay of membrane fusion developed by P. S. Uster and D. W. Deamer (Biochemistry 24, 1-8 (1985)) was used in a study of influenza haemagglutinin-mediated fusion. The characteristics of fusion and haemolysis by X-31 (H3N2) virus, a number of mutants of X-31 which fuse membranes at higher pH,...

journal_title：Virology

authors： Wharton SA,Skehel JJ,Wiley DC

更新日期：1986-02-01 00:00:00

abstract：:The V3 loop of the human immunodeficiency virus type 1 (HIV-1) gp120 exterior envelope glycoprotein (Env) becomes exposed after CD4 binding and contacts the coreceptor to mediate viral entry. Prior to CD4 engagement, a hydrophobic patch located at the tip of the V3 loop stabilizes the non-covalent association of gp120...

journal_title：Virology

authors： Bowder D,Hollingsead H,Durst K,Hu D,Wei W,Wiggins J,Medjahed H,Finzi A,Sodroski J,Xiang SH

更新日期：2018-08-01 00:00:00

abstract：:The coat protein of alfalfa mosaic virus has both structural and regulating functions. The latter is evident from the fact that the genomic RNAs of the virus, although they are of messenger polarity, cannot start an infection cycle in the absence of cost protein. The reason could be that the coat protein is needed for...

journal_title：Virology

authors： De Graaff M,Man in't Veld MR,Jaspars EM

更新日期：1995-04-20 00:00:00

abstract：:The objective of this study was to identify porcine reproductive and respiratory syndrome virus (PRRSV)-encoded proteins that are responsible for the inhibition of TNF-α expression and the mechanism(s) involved in this phenomenon. Using a TNF-α promoter reporter system, the non-structural protein 1 (Nsp1) was found to...

journal_title：Virology

authors： Subramaniam S,Kwon B,Beura LK,Kuszynski CA,Pattnaik AK,Osorio FA

更新日期：2010-10-25 00:00:00

abstract：:Vesicular stomatitis virus (VSV) isolated from two independently established lines of persistently infected mouse L cells expressed an altered phenotype of RNA synthesis at 37 degrees, the temperature at which the persistently infected cultures were maintained (T.K. Frey and J.S. Youngner, 1982, J. Virol. 44, 167-174)...

journal_title：Virology

authors： Frey TK,Youngner JS

更新日期：1984-07-15 00:00:00

abstract：:La Crosse (LAC) virions exposed to different pHs (7.3, 6.2, and 5.4) and temperatures (37 degrees, 20 degrees, and 4 degrees) were preserved in thin layers of vitreous ice and observed by electron cryomicroscopy. Our results indicate that, at lower pH, virus particles interact with each other to form aggregates. In so...

journal_title：Virology

authors： Wang GJ,Hewlett M,Chiu W

更新日期：1991-09-01 00:00:00

abstract：:Multiple nucleoside resistance involves specific mutational patterns of the HIV-1 pol gene that are independent of the classic mutations conferring resistance to individual dideoxynucleosides. These include a cluster of five mutations in the reverse-transcriptase (RT) coding region (A62V, V75I, F77L, F116Y, and Q151M)...

journal_title：Virology

authors： Tamalet C,Yahi N,Tourrès C,Colson P,Quinson AM,Poizot-Martin I,Dhiver C,Fantini J

更新日期：2000-05-10 00:00:00

abstract：:To further our understanding of structure-function relationships within the multifunctional adenovirus DNA binding protein (DBP) a more diverse collection of mutants is necessary. DBP-expressing cell lines (gmDBP) were previously constructed that complemented DBP-negative mutants for viral growth. However, they did no...

journal_title：Virology

authors： Brough DE,Cleghon V,Klessig DF

更新日期：1992-10-01 00:00:00

abstract：:The molecular host response to hepatitis C virus (HCV) infection was examined by isolation of HCV-induced genes from a cDNA library constructed from chimpanzee liver during the acute phase of hepatitis C. Two cDNA clones, 130-7 and 130-51, were obtained by differential hybridization with cDNA probes prepared from poly...

journal_title：Virology

authors： Kato T,Esumi M,Yamashita S,Abe K,Shikata T

更新日期：1992-10-01 00:00:00

abstract：:Purified glycophorin (predominantly type A) from human erythrocytes was found to effectively inhibit reovirus hemagglutination (HA) in contrast to other glycoproteins such as fetuin or ovalbumin. Glycophorin was also a potent inhibitor of reovirus and protein sigma 1 binding to mouse L fibroblasts. Glycophorin pretrea...

journal_title：Virology

authors： Paul RW,Lee PW

更新日期：1987-07-01 00:00:00

abstract：:Little is known about the molecular determinants causing and sustaining viral persistent infections at the cellular level. We found that Drosophila cells persistently infected (PI) with Flock House virus (FHV) invariably harbor defective viral RNAs, which are replicated by the FHV RNA-dependent RNA polymerase. Some de...

journal_title：Virology

authors： Jovel J,Schneemann A

更新日期：2011-10-10 00:00:00

abstract：:Human CD4 was expressed on a range of mammalian cell lines. CD4+ non-primate cells, derived from rat, hamster, mink, cat, and rabbit, bind recombinant gp120 of human immunodeficiency virus type 1 (HIV-1) but are resistant to HIV-1 infection. CD4 expression on various human, rhesus, and African green monkey cell lines ...

journal_title：Virology

authors： Clapham PR,Blanc D,Weiss RA

更新日期：1991-04-01 00:00:00

abstract：:Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutraliz...

journal_title：Virology

authors： Yu B,Dong J,Wang C,Zhan Y,Zhang H,Wu J,Kong W,Yu X

更新日期：2013-03-15 00:00:00

abstract：:Infectious bronchitis has remained one of the most difficult to control diseases in poultry since it was first described in 1931. Previous studies demonstrated that primary CD8(+) T lymphocytes collected at 10 days post-infection (p.i.) are important in controlling acute infection. To further investigate the role of m...

journal_title：Virology

authors： Pei J,Briles WE,Collisson EW

更新日期：2003-02-15 00:00:00

abstract：:H4N8 subtype avian influenza viruses were isolated from shorebirds in eastern Hokkaido. All the isolates shared >99.7% nucleotide homology, and all the viral genes except for PB1 were highly related to those of A/red-necked stint/Australia/1/04. Thus, the isolates were regarded as PB1 reassortants. The most similar PB...

journal_title：Virology

authors： Bui VN,Ogawa H,Xininigen,Karibe K,Matsuo K,Awad SS,Minoungou GL,Yoden S,Haneda H,Ngo LH,Tamaki S,Yamamoto Y,Nakamura K,Saito K,Watanabe Y,Runstadler J,Huettmann F,Happ GM,Imai K

更新日期：2012-02-05 00:00:00

abstract：:The Gardner (GA) and Snyder-Theilen (ST) isolates of feline sarcoma virus (FeSV) represent genetic recombinants between feline leukemia virus (FeLV) and transformation-specific sequences (v-fes gene) of cat cellular origin. A related transforming gene (v-fps), common to the Fujinami, PRC II, and UR 1 strains of avian ...

journal_title：Virology

authors： Groffen J,Heisterkamp N,Shibuya M,Hanafusa H,Stephenson JR

更新日期：1983-03-01 00:00:00

abstract：:Gene transfer into human hematopoietic stem cells using Ad5 is inefficient due to lack of the primary receptor CAR and the secondary receptors alphavbeta3 integrin and alphavbeta5 integrin, and due to the high seroprevalence of Ad5 antibodies in most adults, resulting in diminished gene transduction. In the present st...

journal_title：Virology

authors： Mei YF,Segerman A,Lindman K,Hörnsten P,Wahlin A,Wadell G

更新日期：2004-10-25 00:00:00

abstract：:We report the discovery of Nylanderia fulva virus 1 (NfV-1), the first virus identified and characterized from the ant, Nylanderia fulva. The NfV-1 genome (GenBank accession KX024775) is 10,881 nucleotides in length, encoding one large open reading frame (ORF). Helicase, protease, RNA-dependent RNA polymerase, and jel...

journal_title：Virology

authors： Valles SM,Oi DH,Becnel JJ,Wetterer JK,LaPolla JS,Firth AE

更新日期：2016-09-01 00:00:00

abstract：:Vaccinia topoisomerase provides a model system for structure-function analysis of the type IB topoisomerase family. Here we performed an alanine scan of eight positions in the beta4 and beta5 strands of the N-terminal domain (Leu57, Ile58, Phe59, Val60, Gly61, Ser62, Gln69 and Gly73) and eight positions in the alpha8-...

journal_title：Virology

authors： Tian L,Shuman S

更新日期：2007-03-15 00:00:00

abstract：:The sequences of 189 residues from the 3' terminus of three RNAs of one strain and two RNAs of another strain of tomato aspermy virus have been determined; there was almost complete sequence homology between the RNAs. A base-paired transfer RNA-like structure is proposed for tomato aspermy virus RNAs which is similar ...

journal_title：Virology

authors： Wilson PA,Symons RH

更新日期：1981-07-15 00:00:00

abstract：:A new strain of wound tumor virus (WTV) has been isolated from a periwinkle plant (Catharanthus roseus) that was among several used as bait plants in a blueberry field. The 12 segments of double-stranded RNA of the viral genome were isolated directly from infected tissue and found to have mobilities through agarose ge...

journal_title：Virology

authors： Hillman BI,Anzola JV,Halpern BT,Cavileer TD,Nuss DL

更新日期：1991-12-01 00:00:00

abstract：:An improved purification procedure for human astrovirus serotype 2 (H-Ast2) has facilitated the isolation of a neutralizing monoclonal antibody (PL-2) directed against one of the major structural proteins (VP26) of H-Ast2. A minor component (VP29) of the virus particles is also recognized by PL-2 antibody. Immunofluor...

journal_title：Virology

authors： Sanchez-Fauquier A,Carrascosa AL,Carrascosa JL,Otero A,Glass RI,Lopez JA,San Martin C,Melero JA

更新日期：1994-06-01 00:00:00

abstract：:Infection of mice with coxsackievirus B4 results within days in a severe acute necrotizing pancreatitis, which resolves completely within weeks. Gelatinase B or matrix metalloproteinase 9 (MMP-9) has previously been shown to be involved in several models of pancreatitis, but its role in virus-induced pancreatitis has ...

journal_title：Virology

authors： De Palma AM,Verbeken E,Van Aelst I,Van den Steen PE,Opdenakker G,Neyts J

更新日期：2008-12-05 00:00:00

abstract：:Polyomavirus (PyV) infection induces protective T-cell-independent (TI) IgM and IgG responses in T-cell-deficient (TCR beta x delta-/-) mice. In this study, we show that PyV is a TI -2 antigen: B cells with a mutated Bruton's tyrosine kinase (Xid mutants) do not respond to PyV with antibody secretion in the absence of...

journal_title：Virology

authors： Szomolanyi-Tsuda E,Brien JD,Dorgan JE,Garcea RL,Woodland RT,Welsh RM

更新日期：2001-02-15 00:00:00