Abstract:
:Gene transfer into human hematopoietic stem cells using Ad5 is inefficient due to lack of the primary receptor CAR and the secondary receptors alphavbeta3 integrin and alphavbeta5 integrin, and due to the high seroprevalence of Ad5 antibodies in most adults, resulting in diminished gene transduction. In the present study, we screened six species (species A-F) of adenovirus, displaying different tropisms for interaction with CD34+ cells, at the level of virus attachment and expression. Virus particles were biotinylated and their binding capacity was determined by FACS analysis using streptavidin-FITC. Ad11p, Ad35, and Ad3 (species B) showed high binding affinity, while Ad7, Ad11a (species B), and Ad37 (species D) displayed intermediate affinity. Virions of Ad4 (species E), Ad5 (species C), Ad31 (species A), and Ad41 (species F) hardly bound to hematopoietic progenitor cells. Using a double-labeling system, we demonstrated that adenoviruses bind to quiescent CD34+ cells. Ad11p virions showed the highest affinity among the adenoviruses detected. We further confirmed that virus fiber-specific receptors were present on the hematopoietic progenitor cell surface, because both recombinant fiber of Ad11p and specific antiserum against rfiber could block virus attachment. The ability of the adenoviruses to infect hematopoietic cells was studied by immunofluorescence staining. The adenoviruses from species B and Ad37 showed higher infectivity than Ad31, Ad5, Ad4, and Ad41. Among the studied species B adenoviruses, Ad11p manifested a superior infectivity. Thus, we have confirmed that these cells have high-affinity receptors for species B:2 human adenovirus, Ad11p, and this virus may be used as candidate vector to target therapeutic genes to hematopoietic stem cells.
journal_name
Virologyjournal_title
Virologyauthors
Mei YF,Segerman A,Lindman K,Hörnsten P,Wahlin A,Wadell Gdoi
10.1016/j.virol.2004.07.018subject
Has Abstractpub_date
2004-10-25 00:00:00pages
198-207issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(04)00486-6journal_volume
328pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The nucleotide sequence of the infectious laryngotracheitis virus (ILTV) gene encoding the 205K complex glycoprotein (gp205) was determined. The gene is contained within a 3-kb EcoRI restriction fragment mapping at approximately map coordinates 0.23 to 0.25 in the UL region of the ILTV genome and is transcribed from r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90859-a
更新日期:1991-09-01 00:00:00
abstract::The protruding (P) domain of norovirus VP1 is responsible for immune recognition and host receptor interaction. Our previous studies have demonstrated that a modification of the ends of the P domain affects the conformation and/or function of the P protein. An expression of the P domain with or without the hinge, or w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.11.017
更新日期:2011-02-20 00:00:00
abstract::Cells transformed with specific regions of the foot-and-mouth disease virus (FMDV) genome have been constructed and analyzed with respect to viability and susceptibility to FMDV infection. Constitutive expression of an active protease 3C under the control of the tk promoter has been documented by the ability of transf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1459
更新日期:1995-09-10 00:00:00
abstract::Antiviral cytotoxic T-cells are critical for control of lymphocytic choriomeningitis virus (LCMV) infection in mice. In H-2b mice, the antiviral response is directed against three Db-restricted epitopes in the viral nucleoprotein (NP396-404) and glycoprotein (GP276-286 and GP33-41). Our present data revealed a clear h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8934
更新日期:1998-01-05 00:00:00
abstract::Hepatitis C virus (HCV) is efficient in the establishment of persistent infection. We have previously shown that HCV core protein inhibits T cell proliferation through its interaction with the complement receptor, gC1qR. Here we show that HCV core-induced inhibition of T cell proliferation involves a G(0)/G(1) cell cy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00419-7
更新日期:2003-09-15 00:00:00
abstract::Frequent occurrence of woodchuck hepatitis virus DNA (WHV DNA) integration into or in proximity to myc oncogenes and in the win locus of cellular genome in woodchuck hepatocellular carcinomas (HCC) has been described by several authors. We report a further cellular locus as a recurrent target for WHV integration in wo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.9936
更新日期:1995-12-01 00:00:00
abstract::The HIV-1 transframe protein p6 known to modulate HIV-1 protease activation has been suggested to interact with the viral pathogenicity factor Nef. However, a potential interaction site in p6 has not been mapped so far. To evaluate effects of p6 modification on viral replication in light of Nef function, clustered sub...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.01.042
更新日期:2009-04-25 00:00:00
abstract::A stem-loop structure, formed by a pair of inverted repeats during DNA replication, is a conserved feature at the origin of DNA replication among plant and animal viruses, bacteriophages and plasmids that replicate their genomes via the rolling-circle replication (RCR) mechanism. In this work, a head-to-tail tandem co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.017
更新日期:2007-06-20 00:00:00
abstract::The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography em...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90331-x
更新日期:1987-03-01 00:00:00
abstract::The close association between human papillomavirus type 16 and cervical cancer implies some role for the virus in the development of this cancer. It has been demonstrated that HPV-16 DNA sequences in the presence of activated ras are capable of transforming primary baby rat kidney cells. This communication describes t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90695-2
更新日期:1988-07-01 00:00:00
abstract::We have previously adapted an experimental system which supports autonomous replication of human papillomavirus-11 (HPV-11) genomic DNA in a human squamous carcinoma cell line (SCC-4) following electroporation of linearized viral DNA. This system allows evaluation of HPV-11 transcription in the context of replicating ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0298
更新日期:1996-06-01 00:00:00
abstract::Section of the sciatic nerve during the period of herpes simplex virus (HSV) latent infection was performed to evaluate residual latency in mouse dorsal root ganglion. In control mice without sciatic neurectomy, latency was present in 90-100%, while in those which underwent a neurectomy procedure, latent infection was...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90085-2
更新日期:1988-11-01 00:00:00
abstract::The 5' untranslated region (5'UTR) of coxsackievirus B3 (CVB3) RNA forms a highly ordered secondary structure that has been implicated in controlling initiation of viral translation by internal ribosomal entry. To test this hypothesis, synthetic bicistronic RNAs, with all or part of the 5'UTR in the intercistronic spa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8366
更新日期:1997-02-03 00:00:00
abstract::Studies were performed to induce cross-clade neutralizing antibodies (Abs) by testing various combinations of prime and boost constructs that focus the immune response on structurally-conserved epitopes in the V3 loop of HIV-1 gp120. Rabbits were immunized with gp120 DNA containing a V3 loop characterized by the GPGR ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.039
更新日期:2009-09-15 00:00:00
abstract::The circular single-stranded viral DNA (ssDNA) of filamentous phage is oriented in the virus with a hairpin forming sequence called mos at the leading end of the virion-the end that emerges from the cell first. In the experiments that originally defined mos it was also found to enhance virion production 100-fold, thou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90066-9
更新日期:1988-11-01 00:00:00
abstract::RNase protection analysis of mRNAs for Epstein-Barr virus (EBV) genes activated early in the induction of the productive cycle in Akata cells has confirmed that BZLF1 and BRLF1 behave as immediate early genes. These are likely to be the only virus immediate early genes since analysis of three other transcripts previou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1070
更新日期:1993-02-01 00:00:00
abstract::Mouse hepatitis virus (MHV) JHMV and its soluble receptor-resistant (srr) mutants, srr7, srr11, and srr18, grew and induced syncytia equally well in BHK-R1 cells expressing the MHVR1 receptor derived from MHV-susceptible BALB/c mice. In contrast, srr growth and syncytia formations were drastically reduced relative to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0409
更新日期:2000-07-20 00:00:00
abstract::Marek' disease virus serotype-1, also know as Gallid herpesvirus 2 (GaHV-2), elicits T-cell lymphomas in chickens. The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that Meq homodimers are not sufficient to induce lymphomas in chickens. In this s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.006
更新日期:2010-04-10 00:00:00
abstract::Two genetically distinct infectious recombinant virus clones (pMLV, constructed from Ingelvac PRRS MLV and pMN184, constructed from virulent strain MN184) were developed to study attenuation of contemporary porcine reproductive and respiratory syndrome virus (PRRSV) strain MN184. Two reciprocal chimeric clones (pMLVOR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.09.032
更新日期:2008-02-20 00:00:00
abstract::We measured the effects of non-nucleoside reverse transcriptase (RT) inhibitor-resistant mutations K101E+G190S, on replication fitness and EFV-resistance of HIV(NL4-3). K101E+G190S reduced fitness in the absence of EFV and increased EFV resistance, compared to either single mutant. Unexpectedly, K101E+G190S also repli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.018
更新日期:2010-07-05 00:00:00
abstract::During abortive infection of Go/G1-arrested primary baby mouse kidney (BMK) cell cultures with simian virus 40 (SV40), expression of the viral large T antigen is followed by a mitotic host response including the stimulation of host macromolecular synthesis and induction into the cell cycle of Go/G1-arrested cells. We ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90130-8
更新日期:1985-12-01 00:00:00
abstract::Although rodents are important reservoirs for RNA viruses, to date only one species of rodent coronavirus (CoV) has been identified. Herein, we describe a new CoV, denoted Lucheng Rn rat coronavirus (LRNV), and novel variants of two Betacoronavirus species termed Longquan Aa mouse coronavirus (LAMV) and Longquan Rl ra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.10.017
更新日期:2015-01-01 00:00:00
abstract::The P68 protein kinase (referred to as P68 based on its M(r) of 68,000 in human cells) is a serine/threonine kinase induced by interferon treatment and activated by dsRNAs. The kinase is under tight controls in virus-infected cells since once activated, it phosphorylates its natural substrate eukaryotic initiation fac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90242-h
更新日期:1992-12-01 00:00:00
abstract::Despite significant contributions of monocytes to HIV persistence, the genomic basis of HIV-infection of monocytes and its association with plasma viremia remain elusive. To understand HIV interactions with monocytes during disease progression, monocytic transcriptomes from long-term non-progressors (LTNP), HIV+ patie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.10.026
更新日期:2013-01-20 00:00:00
abstract::Tombusviruses are small, plus-sense, single-stranded RNA viruses of plants. RNA-dependent RNA polymerases (RdRp) of two tombusviruses, Tomato bushy stunt virus (TBSV) and Cucumber necrosis virus (CNV), have been partially purified from infected Nicotiana benthamiana plants. The obtained RdRp complexes are capable of d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0577
更新日期:2000-10-25 00:00:00
abstract::The measles virus (MV) phosphoprotein (P) and V proteins block the interferon (IFN) response by impeding phosphorylation of the signal transducer and activator of transcription 1 (STAT1) by the Janus kinase 1 (JAK1). We characterized how STAT1 mutants interact with P and JAK1 phosphorylation. Certain mutants of the li...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.06.019
更新日期:2013-09-01 00:00:00
abstract::Expression of alphavirus glycoproteins on the surface of infected cells leads to cell fusion after exposure to acidic pH. Two strains of Sindbis virus, AR339 (SV) and neuroadapted Sindbis virus (NSV), which differ in virulence for weanling mice, were found to differ in pH-dependent fusion. BHK-21 cells infected with S...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90178-5
更新日期:1989-04-01 00:00:00
abstract::Sequence heterogeneity of hepatitis B virus (HBV) is increasingly recognized to play a role in virus-host interaction. We have used a recently established method for HBV full-length genome amplification to search for novel types of HBV variants and to investigate further the sequence heterogeneity of HBV genome popula...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8898
更新日期:1997-12-22 00:00:00
abstract::The hemagglutinin of the 2009 pandemic H1N1 influenza virus is a derivative of and is antigenically related to classical swine but not to seasonal human H1N1 viruses. We compared the A/California/7/2009 (CA/7/09) virus recommended by the WHO as the reference virus for vaccine development, with two classical swine infl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.09.009
更新日期:2010-12-05 00:00:00
abstract::Dendrolimus punctatus tetravirus (DpTV) belongs to the genus omegatetravirus of the Alphatetraviridae family. Sequence analysis predicts that DpTV replicase contains a putative helicase domain (Hel). However, the helicase activity in alphatetraviruses has never been formally determined. In this study, we determined th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.08.045
更新日期:2012-11-25 00:00:00