The non-nucleoside reverse transcriptase inhibitor efavirenz stimulates replication of human immunodeficiency virus type 1 harboring certain non-nucleoside resistance mutations.

abstract：:While the 2009 pandemic H1N1 virus has become established in the human population as a seasonal influenza virus, continued adaptation may alter viral virulence. Here, we passaged a 2009 pandemic H1N1 virus (A/Changchun/01/2009) in mice. Serial passage in mice generated viral variants with increased virulence. Adapted ...

journal_title：Virology

authors： Yu Z,Cheng K,Sun W,Zhang X,Li Y,Wang T,Wang H,Zhang Q,Xin Y,Xue L,Zhang K,Huang J,Yang S,Qin C,Wilker PR,Yue D,Chen H,Gao Y,Xia X

更新日期：2015-12-01 00:00:00

abstract：:We cloned the complete complementary DNA of an isolate of the hepatitis C virus, HCV-S1, into a tetracycline-inducible expression vector and stably transfected it into two human hepatoma cell lines, Huh7 and HepG2. Twenty-six Huh7 and two HepG2-positive clones were obtained after preliminary screening. Two Huh7 (SH-7 ...

journal_title：Virology

authors： Lim SP,Soo HM,Tan YH,Brenner S,Horstmann H,MacKenzie JM,Ng ML,Lim SG,Hong Wj

更新日期：2002-11-10 00:00:00

abstract：:The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...

journal_title：Virology

authors： Rota PA,Maes RK,Ruyechan WT

更新日期：1986-10-15 00:00:00

abstract：:In a previous paper (G. Winkler, V. B. Randolph, G. R. Cleaves, T. E. Ryan, and V. Stollar, 1988, Virology 162, 187-196) we showed that the newly synthesized dengue-2 virus nonstructural protein, NS1, exists briefly as a monomer and then undergoes dimerization. We demonstrate here that the dimerization of NS1 is assoc...

journal_title：Virology

authors： Winkler G,Maxwell SE,Ruemmler C,Stollar V

更新日期：1989-07-01 00:00:00

abstract：:The subgroup II luteovirus barley yellow dwarf virus-RPV (BYDV-RPV) acts as a helper virus for a satellite RNA (satRPV RNA). The subgroup II luteovirus beet western yellows virus (BWYV) and the ST9-associated RNA (ST9a RNA), a BWYV-associated RNA that encodes a polymerase similar to those of subgroup I luteoviruses, w...

journal_title：Virology

authors： Rasochová L,Passmore BK,Falk BW,Miller WA

更新日期：1997-05-12 00:00:00

abstract：:Lymphoid cell lines were derived from three avian leukosis virus (ALV)-induced lymphomas. These cell lines contained proviral DNA sequences integrated upstream from the c-myc proto-oncogene, expressed increased levels of c-myc RNA, and were tumorigenic in syngeneic animals. While cell surface immunoglobulin (IgM) was ...

journal_title：Virology

authors： Baba TW,Giroir BP,Humphries EH

更新日期：1985-07-15 00:00:00

abstract：:Loading of most endogenous peptides on major histocompatibility complex class I molecules is conditional on their transport into the endoplasmic reticulum (ER) by the peptide transporter TAP. We describe an HSV-2/1 cross-reactive cytotoxic T-cell (CTL) epitope that is processed in a TAP1-independent manner in vivo fol...

journal_title：Virology

authors： Paliard X,Doe B,Selby MJ,Hartog K,Lee AY,Burke RL,Walker CM

更新日期：2001-03-30 00:00:00

abstract：:Molecular piracy is a biological phenomenon in which one replicon (the pirate) uses the structural proteins encoded by another replicon (the helper) to package its own genome and thus allow its propagation and spread. Such piracy is dependent on a complex web of interactions between the helper and the pirate that occu...

journal_title：Virology

authors： Christie GE,Dokland T

更新日期：2012-12-20 00:00:00

abstract：:The N-terminal domains of the NS1 protein of influenza B virus (NS1B protein) and the NS1 protein of influenza A virus (NS1A protein) share one function: binding double-stranded RNA (dsRNA). Here we show that the N-terminal domain of the NS1B protein possesses an additional function that is not shared by its NS1A coun...

journal_title：Virology

authors： Yuan W,Aramini JM,Montelione GT,Krug RM

更新日期：2002-12-20 00:00:00

abstract：:Foot-and-mouth-disease (FMD) remains the most infectious livestock disease worldwide. Although commercially available inactivated or adenovirus-vectored-vaccines (Ad5-FMD) are effective, they require 5-7 days to induce protection. Therefore, new control strategies that stimulate rapid immune responses are needed. Expr...

journal_title：Virology

authors： Diaz-San Segundo F,Montiel NA,Sturza DF,Perez-Martin E,Hickman D,Ramirez-Medina E,Grubman MJ,de Los Santos T

更新日期：2016-12-01 00:00:00

abstract：:Major efforts have been put forth for the development of effective rotavirus vaccines including transgenic plant vaccines. Previous studies have reported that rotavirus VP7 maintains its neutralizing immunity when it is transformed into the potato genome. The present study was aimed at investigating the hereditary sta...

journal_title：Virology

authors： Li JT,Fei L,Mou ZR,Wei J,Tang Y,He HY,Wang L,Wu YZ

更新日期：2006-12-05 00:00:00

abstract：:Genetic exchanges constitute a significant means by which bacteriophages acquire novel characteristics. Phages of Lactococcus lactis occupy a particular niche, the dairy factory environment, where their populations are subjected to constant changes. Little is known about the mechanisms of evolution that lead to the ge...

journal_title：Virology

authors： Bouchard JD,Moineau S

更新日期：2000-04-25 00:00:00

abstract：:Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...

journal_title：Virology

authors： Struthers JK,Swanepoel R,Shepherd SP

更新日期：1984-04-15 00:00:00

abstract：:Hemorrhagic fever viruses are associated with rapidly progressing severe disease with high case fatality, making them of public health and biothreat importance. Effective antivirals are not available for most of the members of this diverse group of viruses. A broad spectrum strategy for antiviral development would be ...

journal_title：Virology

authors： Spiropoulou CF,Ranjan P,Pearce MB,Sealy TK,Albariño CG,Gangappa S,Fujita T,Rollin PE,Nichol ST,Ksiazek TG,Sambhara S

更新日期：2009-09-15 00:00:00

abstract：:We described a simple method to introduce site-specific mutations into region E3 of adenovirus (Ad). Mutations are made in cloned Ad2 EcoRI-D (map position 76-83), then ligated between Ad5 EcoRI-A (map position 0-76) and EcoRI-B (map position 83-100) to complete the viral genome. We have used this method to isolate a ...

journal_title：Virology

authors： Wold WS,Deutscher SL,Takemori N,Bhat BM,Magie SC

更新日期：1986-01-15 00:00:00

abstract：:The v-Rel oncoprotein of the Rev-T retrovirus interacts with a number of cellular proteins in transformed chicken spleen cells including p40/I kappa B alpha, p68c-Rel, hsc70, and the p124 and p115 precursors for the p50 and p52 subunits of NF kappa B. Here we report that v-Rel associates with at least three other cell...

journal_title：Virology

authors： Xu X,Gélinas C

更新日期：1995-03-10 00:00:00

abstract：:By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and pa...

journal_title：Virology

authors： Hantz O,Baginski I,Fourel I,Chemin I,Trepo C

更新日期：1992-09-01 00:00:00

abstract：:Bovine herpesvirus 1 (BHV-1) gene expression was examined by RNA blot hybridization using clones representing immediate-early, early, and late genes. An immediate-early protein gene probe hybridized with two transcripts, 3.4 and 5.8 kb, expressed by infected cells in the presence of cycloheximide (CH). During infectio...

journal_title：Virology

authors： Seal BS,Whetstone CA,Zamb TJ,Bello LJ,Lawrence WC

更新日期：1992-05-01 00:00:00

abstract：:Mutants of the influenza virus A/seal/Mass/1/80 (H7N7) are described which contain an insertion of 60 nucleotides in the hemagglutinin (HA) gene, derived most probably by recombination between the HA gene and the nucleoprotein gene of the same virus. The nonhomologous RNA recombination resulted in an enhanced hemagglu...

journal_title：Virology

authors： Orlich M,Gottwald H,Rott R

更新日期：1994-10-01 00:00:00

abstract：:Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribo...

journal_title：Virology

authors： Milner JJ,Jackson AO

更新日期：1979-08-01 00:00:00

abstract：:Evidence is presented for the glycosylation of the cauliflower mosaic virus (CaMV) capsid polypeptide. Fluorescent derivatives of concanavalin A (Con A) were shown to bind specifically to the dissociated CaMV capsid polypeptides in polyacrylamide gels. Con A also bound specifically to intact CaMV, so inhibiting the at...

journal_title：Virology

authors： Du Plessis DH,Smith P

更新日期：1981-03-01 00:00:00

abstract：:The simian hemorrhagic fever virus (SHFV) genome differs from those of other members of the family Arterivirus in encoding two adjacent sets of four minor structural protein open reading frames (ORFs). A stable, full-length, infectious SHFV-LVR cDNA clone was constructed. Virus produced from this clone had replication...

journal_title：Virology

authors： Vatter HA,Di H,Donaldson EF,Baric RS,Brinton MA

更新日期：2014-08-01 00:00:00

abstract：:The baculovirus-insect cell system is used routinely for foreign glycoprotein production, but the precise nature of the N-glycosylation pathway in this system remains unclear. Some studies indicate that these cells cannot process N-linked oligosaccharides to complex forms containing outer-chain galactose and sialic ac...

journal_title：Virology

authors： Jarvis DL,Finn EE

更新日期：1995-10-01 00:00:00

abstract：:We have investigated a novel strategy for coexpressing two genes from a retroviral vector. The 5' nontranslated leader region of at least some picornavirus RNAs contains a sequence that can act as an internal ribosome entry site allowing initiation of translation at a downstream AUG codon in a 5' cap-independent manne...

journal_title：Virology

authors： Koo HM,Brown AM,Kaufman RJ,Prorock CM,Ron Y,Dougherty JP

更新日期：1992-02-01 00:00:00

abstract：:Thirteen viable insertion mutants of vaccinia virus have been constructed. These mutants, containing coding sequences of the herpes simplex virus thymidine kinase (HSV-TK) gene, were generated by marker transfer via in vivo recombination. The mutants were identified using a replica filter plating technique by in situ ...

journal_title：Virology

authors： Perkus ME,Panicali D,Mercer S,Paoletti E

更新日期：1986-07-30 00:00:00

abstract：:To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...

journal_title：Virology

authors： Andino Fde J,Grayfer L,Chen G,Chinchar VG,Edholm ES,Robert J

更新日期：2015-11-01 00:00:00

abstract：:The P68 protein kinase (referred to as P68 based on its M(r) of 68,000 in human cells) is a serine/threonine kinase induced by interferon treatment and activated by dsRNAs. The kinase is under tight controls in virus-infected cells since once activated, it phosphorylates its natural substrate eukaryotic initiation fac...

journal_title：Virology

authors： Barber GN,Tomita J,Garfinkel MS,Meurs E,Hovanessian A,Katze MG

更新日期：1992-12-01 00:00:00

abstract：:We have used cells infected with the HIV-1 molecular clone HX10 to study the binding of monoclonal antibodies (mAbs) to different epitopes within the extracellular domain of the HIV-1 transmembrane glycoprotein gp41. Gp41 mAb binding to the infected cells at 4 degrees was variable but weaker than the binding of an ant...

journal_title：Virology

authors： Sattentau QJ,Zolla-Pazner S,Poignard P

更新日期：1995-01-10 00:00:00

abstract：:Human parvovirus B19 (B19) infects human erythroid lineage cells. Accumulating evidence also shows that B19 is detectable in nonerythroid lineage cells in vivo, but the mechanism of infection is still not clear. In this study, we explored the mode of B19 infection of human monocytic cell line U937. An in vitro infecti...

journal_title：Virology

authors： Munakata Y,Kato I,Saito T,Kodera T,Ishii KK,Sasaki T

更新日期：2006-02-05 00:00:00

abstract：:Binding of simian virus 40 (SV40) large T antigen to human and calf thymus topoisomerase I (topo I) was readily detected by using modified enzyme-linked immunosorbent assays and immunoblots. In addition to WT T antigen, binding could also be readily demonstrated with T antigen fragments from the amino-terminal region ...

journal_title：Virology

authors： Simmons DT,Melendy T,Usher D,Stillman B

更新日期：1996-08-15 00:00:00