Sequence complementarity of sonchus yellow net virus RNA with RNA isolated from the polysomes of infected tobacco.

Abstract:

:Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribosomal RNA lacking poly(A) hybridized to approximately 40-60% of the genome. The kinetics of hybridization of all three fractions are best explained by the presence of a single abundance class of viral-complementary RNA. However, titration hybridization of poly(A)+ RNA to an excess of SYNV RNA suggested that viral-complementary sequences which contain poly(A) may vary in concentration over a factor of about fivefold. About 1.5 to 4.6% of the fraction containing poly(A), 0.02 to 0.06% of the fraction lacking poly(A) and 0.04 to 0.18% of the total polyribosomal RNA was complementary to viral RNA as estimated from the kinetics of hybridization. The viral complementary RNA(vcRNA) was heterogeneous in size with a modal sedimentation coefficient of 12 S and a profile in sucrose density gradients similar to the polyadenylated polyribosomal RNA.

journal_name

Virology

journal_title

Virology

authors

Milner JJ,Jackson AO

doi

10.1016/0042-6822(79)90375-1

subject

Has Abstract

pub_date

1979-08-01 00:00:00

pages

90-9

issue

1

eissn

0042-6822

issn

1096-0341

pii

0042-6822(79)90375-1

journal_volume

97

pub_type

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