当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Effect of 5' and 3' terminal sequences, overall length, and coding capacity on the accumulation of defective RNAs associated with broad bean mottle bromovirus in planta.

Effect of 5' and 3' terminal sequences, overall length, and coding capacity on the accumulation of defective RNAs associated with broad bean mottle bromovirus in planta.

Abstract：

:Broad bean mottle bromovirus (BBMV) was shown to accumulate RNA2-derived defective interfering (DI) RNAs [Romero et al., Virology 194, 576-584 (1993); Pogany et al., Virology 212, 574-586 (1995)]. In this work, we utilize three sets of BBMV RNA2-derived artificial DI RNA constructs to determine factors that affect the accumulation of defective RNAs in planta. One set of deletion constructs was used to localize sequences required for efficient accumulation within the 5' 883 nt and the 3' 387 nt of the DI RNAs. The second set had a gradually increasing size of 3' nested deletions to determine the minimal length required for efficient DI RNA accumulation. The smallest DI RNA still accumulating in plants was found to be 1712 nt long. The third set consisted of frameshift mutants which showed that at least 64.4% of BBMV DI RNA sequences must have the 5' portion of the 2a open reading frame to ensure efficient accumulation. The importance of these factors in the selection of DI RNAs is discussed.

journal_name

Virology

journal_title

Virology

authors

Pogany J,Romero J,Bujarski JJ

doi

10.1006/viro.1996.8377

subject

Has Abstract

pub_date

1997-02-17 00:00:00

pages

236-43

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(96)98377-4

journal_volume

228

pub_type

杂志文章

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