Abstract:
:The transcription/replication activity of ribonucleoproteins derived from influenza A primary isolates of human (A/Paris/908/97) or avian origin (A/Mallard/Marquenterre/MZ237/83, A/Hong Kong/156/97) was compared upon reconstitution in mammalian or avian cells, using viral-like reporter RNAs synthesized under the control of the human and chicken RNA polymerase I promoters, respectively. In avian cells, transcription/replication activities were in the same range with all ribonucleoproteins tested. In human cells, ribonucleoproteins derived from A/Mallard/Marquenterre/MZ237/83 showed reduced transcription/replication activity and reduced NP binding to the PB1-PB2-PA complex (P) or to the isolated PB2 subunit, as compared to the ribonucleoproteins derived from A/Paris/908/97. Both defects were restored when PB2 residue Glu-627 was changed to a Lys. Ribonucleoproteins derived from the human A/Hong Kong/156/97 H5N1 isolate showed efficient NP-P interaction in human cells, and high levels of activity which were determined mostly by the PB2 and PA proteins. Our data suggest that PB2 might play a pivotal role in molecular interactions involving both the viral nucleoprotein and cellular proteins.
journal_name
Virologyjournal_title
Virologyauthors
Labadie K,Dos Santos Afonso E,Rameix-Welti MA,van der Werf S,Naffakh Ndoi
10.1016/j.virol.2006.12.027subject
Has Abstractpub_date
2007-06-05 00:00:00pages
271-82issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00934-2journal_volume
362pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The complete genomes of 30 Soybean mosaic virus (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.007
更新日期:2009-10-10 00:00:00
abstract::Virions of southern bean mosaic virus (SBMV) show two distinct sensitivity patterns upon heating. Infectivity loss and capsid denaturation occur concurrently if virions are exposed at 50-55 degrees in 0.1 M sodium phosphate buffer, pH 7.5, or 0.1 M glycine-phosphate buffer, pH 9.0. Contrastingly, virions are inactivat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90437-2
更新日期:1981-01-30 00:00:00
abstract::Human papillomavirus type 27 (HPV-27) and HPV-2c are isolates of the same virus as judged by genomic sequence identity. In a 400-bp stretch of the long control region and the E6 and L1 genes no changes were found between HPV-27 and HPV-2c whereas 55 changes were found between HPV-27 and HPV-2a. This justifies the elev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1307
更新日期:1994-06-01 00:00:00
abstract::West Nile virus (WNV) is a flavivirus that swept rapidly across North America in 1999, declined in prevalence, and then resurged in 2012. To date, no vaccine is available to prevent infection in the human population. Herpes simplex virus (HSV) replication-defective vaccine vectors induce a durable immunity characteriz...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.006
更新日期:2016-09-01 00:00:00
abstract::The effect of rev (art/trs) gene on the level of HIV-1 envelope (env) expression using recombinant adenovirus was investigated. Recombinant adenoviruses expressing either the envelope or the rev gene of the human immunodeficiency virus type 1 (HIV-1) were constructed by inserting the gene into an expression cassette. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90438-w
更新日期:1990-04-01 00:00:00
abstract::Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.05.023
更新日期:2014-09-01 00:00:00
abstract::Foot-and-mouth-disease (FMD) remains the most infectious livestock disease worldwide. Although commercially available inactivated or adenovirus-vectored-vaccines (Ad5-FMD) are effective, they require 5-7 days to induce protection. Therefore, new control strategies that stimulate rapid immune responses are needed. Expr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.09.027
更新日期:2016-12-01 00:00:00
abstract::We have studied somatically murine leukemia proviral integrations in primary N-methyl-N-nitrosourea (MNU)-induced thymic lymphomas of AKR/J mice. The majority of MNU-induced lymphomas contain newly acquired murine leukemia proviral sequences. In contrast to spontaneous AKR/J lymphomas which contain multiple integratio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90638-1
更新日期:1988-05-01 00:00:00
abstract::We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had similar error rates for single nucleotide substitutions; however, PFV RT did not appear to make errors at specific hotspots, like HIV-1 RT. In a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.05.034
更新日期:2007-10-25 00:00:00
abstract::The HSV-1 genome is composed of two unique regions (UL and US) flanked by inverted repeats. During the course of DNA replication the two unique regions UL and US invert relative to one another. In this report we present evidence that cleavage is not necessary for genomic inversion to occur. We isolated and characteriz...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0668
更新日期:1996-12-15 00:00:00
abstract::Turnip leaves infected with turnip crinkle virus accumulate a 35-kd polypeptide which comigrates with the major protein from isolated virions. RNA from TCV virions directs the synthesis of a number of polypeptides in vitro including a 35-kd protein which is immunoprecipitable with antiserum to virus particles. Transla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90326-9
更新日期:1982-04-15 00:00:00
abstract::Mouse LTK- cells (H-2k) were transfected with a series of recombinant plasmids consisting of the herpes simplex virus type 1 thymidine kinase (TK) gene linked to fragments of SV40 DNA coding for portions of SV40 T antigen in pBR322, and TK+ transformants (LTK+) were selected in HAT medium. The TK+ transformants were a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90259-3
更新日期:1983-07-30 00:00:00
abstract::Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis. As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.022
更新日期:2018-02-01 00:00:00
abstract::In this study, we present enzymatic differences found between recombinant RTs derived from feline leukemia virus and feline immunodeficiency virus. Firstly, FIV RT showed low steady state K(m) values for dNTPs compared to FeLV RT. Consistent with this, FIV RT synthesized DNA more efficiently than FeLV RT at low dNTP c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.02.010
更新日期:2005-04-25 00:00:00
abstract::In an attempt to develop an in vitro human cytomegalovirus (HCMV) latency model system, the growth characteristics of HCMV in a human thyroid papillary carcinoma cell line (TPC-1) were examined. When TPC-1 cultures preheated at 40.5 degrees for 48 hr were infected with HCMV and incubated at a supraoptimal temperature ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90171-1
更新日期:1987-11-01 00:00:00
abstract::The major and minor nucleoprotein components in turnip yellow mosaic virus (TYMV) preparations have been fractionated by four cycles of CsCl gradient equilibrium centrifugations. RNAs isolated from the various fractions were translated in a rabbit reticulocyte cell-free system. Three classes of RNAs were studied: geno...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90170-3
更新日期:1979-07-15 00:00:00
abstract::The physical stability of virions of Cucumber mosaic virus (CMV) mutants was investigated to determine if relative stability correlated with efficiency of aphid transmission. Virion stability was evaluated by a urea disruption assay and by testing the infectivity of virus following purifications. All viruses were infe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.11.021
更新日期:2005-02-05 00:00:00
abstract::Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In this paper, we demonstrate that p33 is phosphorylated in vivo and in vitro by ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.08.006
更新日期:2005-12-05 00:00:00
abstract::Human cytomegalovirus (HCMV) was recently shown to encode a large number of spliced mRNAs. While the nuclear export of unspliced viral transcripts has been extensively studied, the role of host mRNA export factors in HCMV mRNA trafficking remains poorly defined. We found that the eIF4AIII RNA helicase, a component of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.12.009
更新日期:2016-02-01 00:00:00
abstract::Foot-and-mouth disease virus protease 3C is essential for the processing of the viral precursor polyprotein. It is shown here to also inhibit gene expression in baby hamster kidney cells after transient expression from transfected cDNA fragments. Protease 3C could not be detected by indirect immunofluorescence in cont...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90090-e
更新日期:1990-02-01 00:00:00
abstract::H5 and H7 avian influenza viruses can become highly pathogenic in chickens after interspecies transmission. These viruses have transmitted directly to humans from birds in Eurasia and Africa (H5N1), the Netherlands (H7N7), and Canada (H7N3). Here we report antigenic, sequence, and phylogenetic analyses of H7N3 viruses...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.008
更新日期:2009-08-01 00:00:00
abstract::Coxsackievirus B3 (CVB3) is nonenveloped and has a single-stranded positive-sense RNA genome. CVB3 induces myocarditis and ultimately dilated cardiomyopathy. Although there are mounting evidences of an interaction between CVB3 particles and the cellular receptors, coxsackievirus and adenovirus receptor (CAR) and decay...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.12.010
更新日期:2005-03-01 00:00:00
abstract::Alpha human papillomaviruses (HPVs) are among the most common sexually transmitted agents of which a subset causes cervical neoplasia and cancer in humans. Alpha-PVs have also been identified in non-human primates although few studies have systematically characterized such types. We cloned and characterized 10 distinc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.012
更新日期:2009-10-25 00:00:00
abstract::Equid herpesvirus-1 (EHV-1) outbreaks continue despite widely used vaccination. We demonstrated previously that an ORF1/ORF71 gene deletion mutant of the EHV-1 strain Ab4 (Ab4ΔORF1/71) is less virulent than its parent Ab4 virus. Here, we describe the Ab4 challenge infection evaluating protection induced by the Ab4ΔORF...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.03.014
更新日期:2019-05-01 00:00:00
abstract::V-CATH, a cathepsin L-like cysteine protease encoded by the baculovirus Autographa californica M nucleopolyhedrovirus, has been shown to play an essential role in host liquefaction. Similar to cellular cathepsin L, V-CATH is synthesized as an inactive proenzyme and is activated by cleavage of the propeptide. Previous ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1378
更新日期:2002-05-10 00:00:00
abstract::The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.01.022
更新日期:2017-04-01 00:00:00
abstract::Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identifie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1147
更新日期:1993-03-01 00:00:00
abstract::Adenovirus 12 (Ad12), but not adenovirus 2 or 5, induces metaphase chromosome fragility at four specific loci in humans: RNU1, RNU2, PSU1, and RN5S. As each of these sites corresponds to a tandemly repeated multigene family encoding a small, abundant structural RNA, we proposed that Ad12 hinders metaphase chromatin co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9512
更新日期:1999-02-01 00:00:00
abstract::Nucleotide sequence analysis of the genome of the baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) identified 18 homologues of the Autographa californica NPV (AcNPV) lefs (late expression factor genes). These BmNPV lefs showed high (73-98%) amino acid sequence identities to AcNPV lefs and were localized to s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8457
更新日期:1997-03-31 00:00:00
abstract::The transmembrane envelope (TM) proteins of retroviruses are used as antigen in diagnostic immunoassays and they represent a conserved target for neutralizing antibodies. To analyze the situation in infections with the feline foamy virus (FFV), its recombinant TM protein was produced and used for ELISA and Western blo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.01.023
更新日期:2011-04-10 00:00:00