当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Virion stability and aphid vector transmissibility of Cucumber mosaic virus mutants.

Virion stability and aphid vector transmissibility of Cucumber mosaic virus mutants.

Abstract：

:The physical stability of virions of Cucumber mosaic virus (CMV) mutants was investigated to determine if relative stability correlated with efficiency of aphid transmission. Virion stability was evaluated by a urea disruption assay and by testing the infectivity of virus following purifications. All viruses were infectious when purified using a low salt buffer without organic solvent, whereas two of seven viruses were less stable and inactivated following purification with a high salt buffer and chloroform. These two viruses were both reassortants derived from the spontaneous transmission-defective mutant CMV-M (F1F2M3 and F1F2M3-L129P). F1F2M3 was relatively unstable, being disrupted between 0 and 1 M urea versus the wild-type CMV-Fny (F1F2M3) that was destabilized at 3-4 M urea. Modifications of F1F2M3 at three amino acid positions (129, 162, 168), singly or in combination, increased the relative stability of virions. A second class of transmission-defective CMVs with engineered mutations in the betaH-betaI surface loop of the CMV-Fny capsid protein (CP) exhibited near wild-type levels of stability. Lastly, a single Pro to Leu substitution at CP position 129 of CMV-Fny (F1F2M3-P129L) conferred the induction of necrosis in tobacco plants and reduced aphid transmissibility, but did not markedly alter the physical stability of virions. Thus, only among CMV-M derivatives harboring the CP mutation of Thr to Ala at position 162 were increases in stability correlated with restoration of transmissibility by the aphid Aphis gossypii.

journal_name

Virology

journal_title

Virology

authors

Ng JC,Josefsson C,Clark AJ,Franz AW,Perry KL

doi

10.1016/j.virol.2004.11.021

subject

Has Abstract

pub_date

2005-02-05 00:00:00

pages

397-405

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(04)00783-4

journal_volume

332

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • JNK phosphorylation, induced during dengue virus infection, is important for viral infection and requires the presence of cholesterol.

    abstract：:Infection with a broad diversity of viruses often activates host cell signaling pathways including the mitogen-activated protein kinase pathway. The present study established that dengue virus infection of human macrophages activates Jun NH(2)-terminal kinase (JNK) and the p38 MAPKs pathways. The activation was observ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.10.019

    authors： Ceballos-Olvera I,Chávez-Salinas S,Medina F,Ludert JE,del Angel RM

    更新日期：2010-01-05 00:00:00

  • Cytosine deamination and selection of CpG suppressed clones are the two major independent biological forces that shape codon usage bias in coronaviruses.

    abstract：:Using the complete genome sequences of 19 coronavirus genomes, we analyzed the codon usage bias, dinucleotide relative abundance and cytosine deamination in coronavirus genomes. Of the eight codons that contain CpG, six were markedly suppressed. The mean NNU/NNC ratio of the six amino acids using either NNC or NNU as ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.08.010

    authors： Woo PC,Wong BH,Huang Y,Lau SK,Yuen KY

    更新日期：2007-12-20 00:00:00

  • Glycosylation within an antigenic site on the HN glycoprotein of Newcastle disease virus interferes with its role in the promotion of membrane fusion.

    abstract：:The binding of monoclonal antibodies to antigenic site 3 on the hemagglutinin-neuraminidase (HN) glycoprotein of Newcastle disease virus neutralizes viral infectivity and prevents syncytium formation by a mechanism other than the prevention of viral attachment. The virus can escape neutralization by these antibodies b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1506

    authors： Deng R,Wang Z,Glickman RL,Iorio RM

    更新日期：1994-10-01 00:00:00

  • Susceptibility to infection by the human immunodeficiency virus (HIV) correlates with T4 expression in a parental monocytoid cell line and its subclones.

    abstract：:The monocytoid tumor cell line U-937 and five derived subclones were infected with the HTLV-IIIB isolate of the human immunodeficiency virus (HIV). Susceptibility to infection and sensitivity to the cytopathic effects correlated with the expression of the T4 antigen on the cell surface. On the basis of these character...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90278-9

    authors： Asjö B,Ivhed I,Gidlund M,Fuerstenberg S,Fenyö EM,Nilsson K,Wigzell H

    更新日期：1987-04-01 00:00:00

  • Analysis of the SH3-binding region of HIV-1 nef: partial functional defects introduced by mutations in the polyproline helix and the hydrophobic pocket.

    abstract：:An SH3-binding domain within the Nef protein of primate lentiviruses has been reported to be important to viral replication and infectivity and dispensable for CD4 downregulation, but its precise role remains unclear. This study investigates the effects of mutations in both the polyproline helix and in the hydrophobic...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9897

    authors： Craig HM,Pandori MW,Riggs NL,Richman DD,Guatelli JC

    更新日期：1999-09-15 00:00:00

  • Genetic analysis of bipartite geminivirus tissue tropism.

    abstract：:The bipartite geminiviruses bean golden mosaic virus (BGMV), cabbage leaf curl virus (CabLCV), and tomato golden mosaic virus (TGMV) exhibit differential tissue tropism in Nicotiana benthamiana. In systemically infected leaves, BGMV remains largely confined to vascular-associated cells (phloem-limited), whereas CabLCV...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1205

    authors： Qin Y,Petty IT

    更新日期：2001-12-20 00:00:00

  • Isolation and preliminary characterization of temperature-sensitive mutants of human cytomegalovirus.

    abstract：:Thirteen temperature-sensitive (ts) mutants of human cytomegalovirus (HCMV) have been isolated after mutagenesis with nitrosoguanidine or ultraviolet light. Four mutants defective in viral DNA synthesis at nonpermissive temperature (DNA-) were classified into two separate complementation groups. The other nine HCMV ts...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90257-1

    authors： Dion M,Hamelin C

    更新日期：1987-05-01 00:00:00

  • Filamentous phage morphogenetic signal sequence and orientation of DNA in the virion and gene-V protein complex.

    abstract：:The circular single-stranded viral DNA (ssDNA) of filamentous phage is oriented in the virus with a hairpin forming sequence called mos at the leading end of the virion-the end that emerges from the cell first. In the experiments that originally defined mos it was also found to enhance virion production 100-fold, thou...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90066-9

    authors： Bauer M,Smith GP

    更新日期：1988-11-01 00:00:00

  • SYNCRIP (synaptotagmin-binding, cytoplasmic RNA-interacting protein) is a host factor involved in hepatitis C virus RNA replication.

    abstract：:Hepatitis C virus (HCV) RNA replication requires viral nonstructural proteins as well as cellular factors. Recently, a cellular protein, synaptotagmin-binding, cytoplasmic RNA-interacting protein (SYNCRIP), also known as NSAP1, was found to bind HCV RNA and enhance HCV IRES-dependent translation. We investigate whethe...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.01.018

    authors： Liu HM,Aizaki H,Choi KS,Machida K,Ou JJ,Lai MM

    更新日期：2009-04-10 00:00:00

  • The role of proximal and distal sequence variations in the presentation of an immunodominant CTL epitope encoded by the ecotropic AK7 MuLV.

    abstract：:An emv-14-derived, replication-competent ecotropic murine leukemia virus [MuLV], designated AK7, was previously cloned from the AKXL-5 recombinant inbred mouse strain and partially characterized. While genetically encoding for an envelope-derived immunodominant CTL epitope [KSPWFTTL] located in the transmembrane regio...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8747

    authors： Kim V,Green WR

    更新日期：1997-09-29 00:00:00

  • Dispensability of 3' tRNA-like sequence for packaging cowpea chlorotic mottle virus genomic RNAs.

    abstract：:The 3' ends of three genomic RNAs (gRNAs) of cowpea chlorotic mottle virus (CCMV) terminate in a highly conserved tRNA-like structure (3'TLS). To examine the intrinsic role played the 3'TLS in packaging, the competence of each gRNA lacking the 3' TLS (DeltaTLS-gRNA) to interact with dissociated coat protein (CP) subun...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.12.009

    authors： Annamalai P,Rao AL

    更新日期：2005-02-20 00:00:00

  • Effect of exogenous interferon and an interferon inducer on western equine encephalitis virus disease in a hamster model.

    abstract：:Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infecti...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.10.031

    authors： Julander JG,Siddharthan V,Blatt LM,Schafer K,Sidwell RW,Morrey JD

    更新日期：2007-04-10 00:00:00

  • Molecular studies on bromovirus capsid protein. VII. Selective packaging on BMV RNA4 by specific N-terminal arginine residuals.

    abstract：:An arginine-rich RNA-binding motif (ARM) found at the N-proximal region of Brome mosaic virus (BMV) coat protein (CP) adopts alpha-helical conformation and shares homology with CPs of plant and insect RNA viruses, HIV-Rev and Tat proteins, bacterial antiterminators, and ribosomal splicing factors. The ARM of BMV CP, c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0513

    authors： Choi YG,Rao AL

    更新日期：2000-09-15 00:00:00

  • Effects of tumor necrosis factor on viral replication and pulmonary inflammation during acute mouse adenovirus type 1 respiratory infection.

    abstract：:CD8 T cells contribute to effective clearance of mouse adenovirus type 1 (MAV-1) and to virus-induced pulmonary inflammation. We characterized effects of a CD8 T cell effector, TNF, on MAV-1 pathogenesis. TNF inhibited MAV-1 replication in vitro. TNF deficiency or immunoneutralization had no effect on lung viral loads...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.05.004

    authors： Pant K,Chandrasekaran A,Chang CJ,Vageesh A,Popkov AJ,Weinberg JB

    更新日期：2020-08-01 00:00:00

  • The E5 gene of HPV-16 enhances keratinocyte immortalization by full-length DNA.

    abstract：:Human papillomavirus type 16 (HPV-16) is the most common papillomavirus genome found in human cervical cancers and its DNA is capable of immortalizing primary keratinocytes in vitro. When expressed by their native promoter, two separate HPV-16 oncogenes, E6 and E7, cooperate to immortalize primary human keratinocytes....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0475

    authors： Stöppler MC,Straight SW,Tsao G,Schlegel R,McCance DJ

    更新日期：1996-09-01 00:00:00

  • Hepatitis C virus core protein inhibits apoptosis via enhanced Bcl-xL expression.

    abstract：:Previous studies indicated that hepatitis C virus core protein influences cellular apoptosis. However, the precise mechanisms of the effects are not fully understood. Therefore, in this study, we examined the mechanisms of the effects on cell apoptosis by core protein, using transiently transfected and magnetically co...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1371

    authors： Otsuka M,Kato N,Taniguchi H,Yoshida H,Goto T,Shiratori Y,Omata M

    更新日期：2002-04-25 00:00:00

  • Receptor usage of a newly emergent adenovirus type 14.

    abstract：:Recently, cases of severe respiratory illness in military and civilian populations have been associated with a new genomic variant of adenovirus (Ad) serotype 14, designated Ad14a. Compared to the Ad14 reference strain (de Wit), this new virus had a deletion of two amino acid residues in the fiber protein knob. Here w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.02.034

    authors： Wang H,Tuve S,Erdman DD,Lieber A

    更新日期：2009-05-10 00:00:00

  • Antigenic and genetic characterization of Sindbis virus monoclonal antibody escape mutants which define a pathogenesis domain on glycoprotein E2.

    abstract：:The Sindbis virus mutant SB-RL, in contrast to its parent, Sindbis strain AR339 (SB), is attenuated in neonatal mice, has an increased rate of penetration in tissue culture cells, and is more sensitive to neutralization by E2-specific monoclonal antibodies (MCAbs) R6 and R13. These phenotypic differences are controlle...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90184-s

    authors： Pence DF,Davis NL,Johnston RE

    更新日期：1990-03-01 00:00:00

  • Multiple origins of the complementary defective genomes of RF and origin proximal sequences of GS, two human papovavirus isolates.

    abstract：:It has previously been shown that the genome of RF virus, a variant of the human papovavirus, BK, consists of two DNA species, one (R1a) with a deletion corresponding to the early and the other (R2) with a deletion corresponding to the late region of BKV (A. Pater, M.M. Pater, and G. di Mayorca (1980). J. Virol. 36, 4...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90509-3

    authors： Pater A,Pater MM,Chang LS,Slawin K,Di Mayorca G

    更新日期：1983-12-01 00:00:00

  • Varicella-zoster virus infection of human sensory neurons.

    abstract：:Primary varicella-zoster virus (VZV) infection of humans may result in latent infection of sensory neurons in the peripheral nervous system. To examine the interaction of VZV with the sensory neuron we infected immunochemically defined human neurons with cell-associated VZV. Utilizing double-label immunofluorescence t...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90141-8

    authors： Wigdahl B,Rong BL,Kinney-Thomas E

    更新日期：1986-07-30 00:00:00

  • Small RNA-based interactions between rice and the viruses which cause the tungro disease.

    abstract：:Rice tungro disease is caused by a complex of two viruses, Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). To examine the RNAi-based defence response in rice during tungro disease, we characterized the virus-derived small RNAs and miRNAs by Deep Sequencing. We found that, while 21 nt/22 nt...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.07.022

    authors： Zarreen F,Kumar G,Johnson AMA,Dasgupta I

    更新日期：2018-10-01 00:00:00

  • Characterization of west nile virus persistent infections in genetically resistant and susceptible mouse cells. II. Generation of temperature-sensitive mutants.

    abstract：:Long-term persistent infections were established with the flavivirus, West Nile virus (WNV), strain E101, in embryofibroblast cultures derived from susceptible C3H/HE and congenic-resistant C3H/RV mice. Cultures were initially maintained by weekly subculture at 37 degrees, but at passage 6 sister cultures were shifted...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90454-4

    authors： Brinton MA,Davis J,Schaefer D

    更新日期：1985-01-15 00:00:00

  • Altered dinucleotide content within the latently transcribed regions of the DNA of alpha herpes viruses--implications for latent RNA expression and DNA structure.

    abstract：:The alphaherpesviruses establish latent infections in sensory neuronal cells, during which a number of latency-associated RNA transcripts (LATs) are produced. The reasons for the production of the LATs, however, and the mechanism by which the LAT region of the genome remains active during latency are unknown. Here we ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1267

    authors： Coffin RS,Howard MK,Latchman DS

    更新日期：1995-06-01 00:00:00

  • HIV induces homing of resting T lymphocytes to lymph nodes.

    abstract：:In vivo infection with human immunodeficiency virus type 1 (HIV-1) leads to gradual depletion of CD4+ T lymphocytes from the peripheral blood and later from the lymphoid organs. The mechanism of CD4 cell depletion is not known. HIV can only replicate in dividing lymphocytes, but greater than 98% of the lymphocytes in ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8397

    authors： Wang L,Robb CW,Cloyd MW

    更新日期：1997-02-17 00:00:00

  • A model system for regulation of chronic HIV-1 infection in peripheral B lymphocytes.

    abstract：:B-HIV1, an oligoclone of immortalized cells derived from human peripheral B lymphocytes infected in vitro with the TIIIB isolate of HIV-1, produces low levels of replication-competent HIV when propagated in 1% serum, but increases production > or = 5-fold after phorbol myristate acetate (PMA) exposure. Electron micros...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1499

    authors： Laurence J,Grimison B,Rodriguez-Alfageme C,Astrin SM

    更新日期：1993-10-01 00:00:00

  • Q-mediated late gene transcription of bacteriophage lambda: RNA start point and RNase III processing sites in vivo.

    abstract：:The location of the RNA start point of in vivo Q-activated late gene RNA of bacteriophage lambda has been determined to be identical to the start point of in vitro 6 S RNA. The 6 S RNA is made early in infection and is efficiently antiterminated by Q. Two RNase III cut sites are located within Q-dependent RNA sequence...

    journal_title：Virology

    pub_type： 杂志文章

    doi：

    authors： Daniels DL,Subbarao MN,Blattner FR,Lozeron HA

    更新日期：1988-12-01 00:00:00

  • Identification of three genes nonessential for growth in cell culture near the right terminus of the unique sequences of long component of herpes simplex virus 1.

    abstract：:We report the construction of herpes simplex virus 1 recombinants from which all or part of the coding sequences of four open reading frames have been deleted. In recombinants R7101 and R7108, the BamHI D' fragment containing the coding sequences of the dUTPase gene and the promoter-regulatory domain of a late gene wa...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90534-x

    authors： Barker DE,Roizman B

    更新日期：1990-08-01 00:00:00

  • HPV-18 immortalization of human keratinocytes.

    abstract：:The oncogenic potential of human papillomavirus type 18 which is found in a significant number of cervical and penile cancer biopsies was tested in primary human keratinocytes derived from neonatal foreskin. Viral DNA and a gene for resistance to neomycin were introduced into these cells by calcium phosphate transfect...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90247-x

    authors： Kaur P,McDougall JK

    更新日期：1989-11-01 00:00:00

  • Immunogenicity of ORFV-based vectors expressing the rabies virus glycoprotein in livestock species.

    abstract：:The parapoxvirus Orf virus (ORFV) encodes several immunomodulatory proteins (IMPs) that modulate host-innate and pro-inflammatory responses and has been proposed as a vaccine delivery vector for use in animal species. Here we describe the construction and characterization of two recombinant ORFV vectors expressing the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.08.027

    authors： Martins M,Joshi LR,Rodrigues FS,Anziliero D,Frandoloso R,Kutish GF,Rock DL,Weiblen R,Flores EF,Diel DG

    更新日期：2017-11-01 00:00:00

  • High levels of SIVmnd-1 replication in chronically infected Mandrillus sphinx.

    abstract：:Viral loads were investigated in SIVmnd-1 chronically infected mandrills and the results were compared with those previously observed in other nonpathogenic natural SIV infections. Four naturally and 11 experimentally SIVmnd-1-infected mandrills from a semi-free-ranging colony were studied during the chronic phase of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.08.015

    authors： Pandrea I,Onanga R,Kornfeld C,Rouquet P,Bourry O,Clifford S,Telfer PT,Abernethy K,White LT,Ngari P,Müller-Trutwin M,Roques P,Marx PA,Simon F,Apetrei C

    更新日期：2003-12-05 00:00:00