Abstract:
:The binding of monoclonal antibodies to antigenic site 3 on the hemagglutinin-neuraminidase (HN) glycoprotein of Newcastle disease virus neutralizes viral infectivity and prevents syncytium formation by a mechanism other than the prevention of viral attachment. The virus can escape neutralization by these antibodies by the addition of an N-glycan at a site introduced by a D287N mutation in HN. The variant has significantly reduced ability to induce fusion from within, the mode of fusion promoted by the viral glycoproteins deposited on the cell surface late in infection. Conversely, and unlike the parent virus, the variant has acquired the ability to promote fusion from without, the mode of fusion directly mediated by input virions at high multiplicity. This finding is consistent with different roles for the HN protein in virion-cell and cell-cell fusion. D287N-mutated HN with its additional N-glycan shows a markedly reduced ability, compared to wild-type HN, to complement the viral fusion protein in the promotion of fusion in a BHK cell transient expression system. This confirms that the addition of an N-glycan in HN antigenic site 3 and the deficiency in syncytium formation are causally related. Moreover, no alteration in cell surface expression, hemadsorption, or neuraminidase activity was detected in the mutated protein. This monoclonal antibody-selected mutation suggests that a fusion-related function, secondary to receptor recognition, may be defined by the globular head of the HN spike. However, D287C or D287S-mutated HN is as effective as the wild-type protein in the promotion of fusion in the coexpression system. This suggests that the diminished fusogenicity of the D287N-mutated protein is probably due to a more global effect of glycosylation in site 3 rather than an alteration at the amino acid level.
journal_name
Virologyjournal_title
Virologyauthors
Deng R,Wang Z,Glickman RL,Iorio RMdoi
10.1006/viro.1994.1506subject
Has Abstractpub_date
1994-10-01 00:00:00pages
17-26issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71506-6journal_volume
204pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::An essentially complete DNA copy of the ambisense S RNA species of Punta Toro (PT) phlebovirus (T. Ihara, H. Akashi, and D.H.L. Bishop, 1984, Virology 136, 293-306) has been inserted in either orientation into Autographa californica nuclear polyhedrosis baculovirus (AcNPV) in lieu of the 5' coding region of the AcNPV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90276-5
更新日期:1987-04-01 00:00:00
abstract::The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species termed RNA-1 and RNA-2. RNA-2 is required for infection of whole plants but is dispensable for infection and virion formation in protoplasts. We have used full-length cDNA clones of RNA-1 and -2 from which infec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1004
更新日期:1993-01-01 00:00:00
abstract::Infectious pancreatic necrosis is an important viral disease of salmonid fish reared in hatchery. Its etiological agent, IPNV, showed a high degree of antigenic heterogeneity. Up to 10 serotypes and 2 serogroups were proposed. Yet, very little is known about genomic variations among viruses of different origin. In ord...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1441
更新日期:1993-08-01 00:00:00
abstract::The influenza RNA polymerase is known to be important in pathogenicity and adaptation of avian influenza viruses to mammalian hosts. However, the molecular mechanisms responsible are only partly understood. Here we investigated the role of the polymerase in two different, closely related, H5N1 influenza viruses - a hi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.036
更新日期:2010-05-25 00:00:00
abstract::All tissue culture systems for propagating HBV employed so far make use of tandemly arranged HBV genomes usually under the control of strong foreign promoters. Thus these systems are helpful for virus production but are of limited value in the investigation of the regulation of HBV replication or of the extent to whic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0049
更新日期:1996-02-01 00:00:00
abstract::A reporting replicon of West Nile virus (WN) was used to distinguish between the function of the 3' untranslated region (UTR) in viral translation and RNA replication. Deletions of various regions of the 3' UTR of the replicon did not significantly affect viral translation, but abolished RNA replication. A systematic ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.022
更新日期:2005-01-20 00:00:00
abstract::A class of disulfide constrained peptides containing a core motif FPWG was identified from a screen of phage displayed library using the HCV RNA-dependent RNA polymerase (NS5B) as a bait. Surface plasmon resonance studies showed that three highly purified synthetic constrained peptides bound to immobilized NS5B with e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00313-1
更新日期:2003-08-15 00:00:00
abstract::In order to establish whether the poor growth of the two fastidious adenoviruses types 40 and 41 (Ad40 and Ad41) in HeLa cells is due to a reduced trans-activation by the early region 1A (E1A), we have determined the trans-activating effect of this region on the expression of the chloramphenicol acetyltransferase (CAT...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90080-8
更新日期:1987-09-01 00:00:00
abstract::Viruses that establish latent infection must maintain their DNA in the host nucleus through many cellular generations. Here we identify a novel mechanism by which the gammaherpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) may achieve this persistence in latently infected body cavity-based lymphoma (BCBL) cell...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9999
更新日期:1999-11-25 00:00:00
abstract::Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are local...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.12.012
更新日期:2014-02-01 00:00:00
abstract::Plasmids containing the E1 region of Ad12 DNA can transform certain rodent cells into oncogenic cells. To study the role of the E1a subregion in the process of oncogenic transformation, Ad12 region E1 mutants carrying deletions in the E1a region were constructed. Deletion mutants pR7 and pR8 affect only the 13 S mRNA ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90178-2
更新日期:1983-09-01 00:00:00
abstract::Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.027
更新日期:2007-07-05 00:00:00
abstract::Sulfation is a common post-translational modification of secreted and membrane proteins, with the sulfate attached to tyrosine residues or to glycan side-chains. I have shown that varicella-zoster virus (VZV) envelope glycoproteins gpI, gpII, and gpIII can be labeled with [35S]sulfate. The predominant VZV glycoprotein...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1576
更新日期:1993-11-01 00:00:00
abstract::ARGONAUTEs (notably AGO1 and AGO2) are effectors of plant antiviral RNA silencing. AGO1 was shown to be required for the temperature-dependent symptom recovery of Nicotiana benthamiana plants infected with tomato ringspot virus (isolate ToRSV-Rasp1) at 27 °C. In this study, we show that symptom recovery from isolate T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.08.016
更新日期:2018-11-01 00:00:00
abstract::RNase protection analysis of mRNAs for Epstein-Barr virus (EBV) genes activated early in the induction of the productive cycle in Akata cells has confirmed that BZLF1 and BRLF1 behave as immediate early genes. These are likely to be the only virus immediate early genes since analysis of three other transcripts previou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1070
更新日期:1993-02-01 00:00:00
abstract::The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography em...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90331-x
更新日期:1987-03-01 00:00:00
abstract::Ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (HSV-2) DNA sequences. Two vulvar tumors and one vulvar dysplastic tissue were found to contain DNA sequences homologous to the BglII O fragment (coordinates...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90179-0
更新日期:1986-11-01 00:00:00
abstract::Highly pathogenic alphaviruses display complex glycans on their surface. These glycans play a crucial role in viral pathogenesis by facilitating glycan-host interaction during viral entry which can be targeted. Various studies have reported antiviral activity of lectins that bind to the glycans present on the surface ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.10.009
更新日期:2019-01-02 00:00:00
abstract::The long terminal repeat (LTR) of mouse mammary tumor virus (MMTV) encodes a protein which functions as a superantigen. The BALB/cV strain differs from other exogenous MMTVs antigenically, biochemically, on the basis of restriction fragment analysis, and by the specificity of its superantigen for V beta 2+ T cells. In...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1480
更新日期:1993-09-01 00:00:00
abstract::We described a simple method to introduce site-specific mutations into region E3 of adenovirus (Ad). Mutations are made in cloned Ad2 EcoRI-D (map position 76-83), then ligated between Ad5 EcoRI-A (map position 0-76) and EcoRI-B (map position 83-100) to complete the viral genome. We have used this method to isolate a ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90412-5
更新日期:1986-01-15 00:00:00
abstract::Infection with the Tucson strain of coxsackievirus B1 (CVB1T) causes the development of chronic inflammatory myopathy (CIM) and hind limb weakness in susceptible strains of mice. In this study, a panel of six plaque-purified viruses exhibiting either small or large plaque phenotypes was derived from parental CVB1T and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8592
更新日期:1997-06-23 00:00:00
abstract::Efavirenz resistance during HIV-1 treatment failure is usually associated with the reverse transcriptase mutation K103N. L100I, V108I, or P225H can emerge after K103N and increase its level of efavirenz resistance. K103N + L100I is the most drug-resistant of the double mutants but is the least common clinically. We hy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.05.021
更新日期:2006-09-15 00:00:00
abstract::Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deep...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.023
更新日期:2015-11-01 00:00:00
abstract::Mutational analyses were carried out to investigate whether the nuclear inclusion protein a (NIa) C-terminal amino acids of turnip mosaic potyvirus play any roles in the cis-cleavage between NIa and NIb. The processing rate of the NIa-NIb junction sequence was decreased significantly by either V240D or Q243A mutation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8938
更新日期:1998-02-01 00:00:00
abstract::In 2006, an exotic reassortant orbivirus, epizootic hemorrhagic disease virus serotype 6 (EHDV-6) [strain (Indiana)], was first detected in the United States. To characterize the reassortment configuration of this virus and to conclusively determine the parental virus of each RNA segment, the complete genome of EHDV-6...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.12.004
更新日期:2012-03-01 00:00:00
abstract::To better understand the origin of human T-cell leukemia virus type l (HTLV-l) in South America, we conducted a phylogenetic study on 27 new HTLV-ls in Brazil. These were obtained from Brazilians of various ethnic origins, such as Japanese immigrants, whites, blacks and mulattos. We amplified and sequenced proviral DN...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1999-08-15 00:00:00
abstract::The herpes simplex virus single-stranded DNA-binding protein, ICP8, localizes initially to structures in the nucleus called prereplicative sites. As replication proceeds, these sites mature into large globular structures called replication compartments. The details of what signals or proteins are involved in the redis...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00108-9
更新日期:2003-05-10 00:00:00
abstract::The envelope protein of yellow fever virus 17D (YFV-17D) contains a solvent-exposed RGD motif, which has led to the suggestion that integrins may function as cellular receptors for YFV-17D. We found that mutating the RGD motif to RGE had no effect on viral titers, whereas changing RGD to TGD, TGE, TAD, TAE, or RGS led...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0026
更新日期:1999-12-05 00:00:00
abstract::3H-labelled complementary DNA (cDNA) was reverse transcribed from the RNA of three biologically distinct isolates of bean yellow mosaic virus (BYMV-G, Q, and S) by the random primer method of Taylor et al. [Biochim. Biophys. Acta 442, 324-330 (1976)]. Excess virus RNA was hybridized with each of the cDNAs and percenta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90074-x
更新日期:1981-04-30 00:00:00
abstract::The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.11.004
更新日期:2019-01-15 00:00:00