Abstract:
:3H-labelled complementary DNA (cDNA) was reverse transcribed from the RNA of three biologically distinct isolates of bean yellow mosaic virus (BYMV-G, Q, and S) by the random primer method of Taylor et al. [Biochim. Biophys. Acta 442, 324-330 (1976)]. Excess virus RNA was hybridized with each of the cDNAs and percentage hybrid formation was assayed using the single-strand-specific nuclease S1, The R0t (1/2) values obtained for the homologous hybridization reactions (1.2 x 10(-2) mol sec liter(-1)) showed that all three BYMV-RNAs were unique species, without detectable zones of reiteration. Reciprocal hybridizations between isolates G, Q, and S have shown that each was significantly different from the other. A comparison of these three isolates with a selection of other potyviruses showed that the relationship between them was closer than that observed between any of them and the other potyviruses tested.
journal_name
Virologyjournal_title
Virologyauthors
Abu-Samah N,Randles JWdoi
10.1016/0042-6822(81)90074-xsubject
Has Abstractpub_date
1981-04-30 00:00:00pages
436-44issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(81)90074-Xjournal_volume
110pub_type
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