Abstract:
:We have analyzed the limiting factors involved in the induction of human immunodeficiency virus type 1 (HIV-1) provirus expression by tumor necrosis factor-alpha (TNF-alpha), phorbol-12-myristate-13-acetate (PMA), and bryostatin-1 in T-cells (ACH-2) and monocytes (U1). We have demonstrated that, while there is a correlation among the increase of 9.2-kilodalton (kDa) HIV-1 RNA, the increase of viral proteins (p24) in the cells, and the release of HIV-1 virions into the medium, there is no direct correlation between the levels of induced NF-kappa B binding proteins and the expression of HIV-1 provirus. The presence of nuclear NF-kappa B-specific proteins appears to be essential only for the initiation of viral replication, since the HIV-1 transcripts could be detected in TNF-alpha or bryostatin-1-stimulated cells also at later times postinduction, times when no NF-kappa B proteins could be detected in the nucleus. The uv crosslinking of DNA and proteins has shown that TNF-alpha, PMA, and bryostatin-1 induce different sets of NF-kappa B binding proteins with distinct kinetics of binding.
journal_name
Virologyjournal_title
Virologyauthors
Vlach J,Pitha PMdoi
10.1016/0042-6822(92)90295-zsubject
Has Abstractpub_date
1992-03-01 00:00:00pages
63-72issue
1eissn
0042-6822issn
1096-0341journal_volume
187pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The RNA dependent RNA polymerase of Sendai virus consists of a complex of the large (L) and phosphoprotein (P) subunits where L is thought to be responsible for all the catalytic activities necessary for viral RNA synthesis. We previously showed that the L protein forms an oligomer [Smallwood, S., Cevik, B., Moyer, S....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.032
更新日期:2007-06-20 00:00:00
abstract::The influenza RNA polymerase is known to be important in pathogenicity and adaptation of avian influenza viruses to mammalian hosts. However, the molecular mechanisms responsible are only partly understood. Here we investigated the role of the polymerase in two different, closely related, H5N1 influenza viruses - a hi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.036
更新日期:2010-05-25 00:00:00
abstract::The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90094-6
更新日期:1992-01-01 00:00:00
abstract::Transgenic pigs are currently the most favored potential source of organs for xenotransplantation. Like all mammalian species they all harbor endogenous retroviruses in their genome. These porcine endogenous retroviruses (PERVs) are produced from several primary cells and cell lines and are able to infect human cells....
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0149
更新日期:2000-03-01 00:00:00
abstract::Tomato spotted wilt tospovirus (TSWV) is the type member of the plant-infecting viruses of the genus Tospovirus in the family Bunyaviridae. The three TSWV RNAs are encapsidated with nucleocapsid (N) protein to form ribonucleoprotein (RNP) which serves as the template for viral transcription and replication. Regions of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9223
更新日期:1998-08-15 00:00:00
abstract::Tick-borne flaviviruses are maintained in nature in an enzootic cycle involving a tick vector and a vertebrate host. Thus, the virus replicates in two disparate hosts, each providing selective pressures that can influence virus replication and pathogenicity. To identify viral determinants associated with replication i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.030
更新日期:2008-11-25 00:00:00
abstract::Poliovirus procapsids, isolated and partially purified from infected HeLa cells, were found to dissociate into 14 S pentamers following freezing at -20 degrees overnight. Unlike pentameric subunits isolated directly from virus-infected cells or those obtained by alkaline treatment of procapsids, the "freeze-dissociate...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90465-x
更新日期:1987-08-01 00:00:00
abstract::Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90005-v
更新日期:1991-01-01 00:00:00
abstract::In a previous study on the replication of Kunjin virus using immunoelectron microscopy (E. G. Westaway, J. M. Mackenzie, M. T. Kenney, M. K. Jones, and A. A. Khromykh, 1997, J. Virol. 71, 6650-6661), NS1 and NS3 were found associated with double-stranded RNA (dsRNA) within vesicle packets (VP) in infected Vero cells, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9156
更新日期:1998-06-05 00:00:00
abstract::West Nile virus (WNV) is a mosquito-borne flavivirus that causes febrile illness, encephalitis, and occasionally death in humans. The envelope protein is the main component of the WNV virion surface, and domain III of the envelope protein (EIII) is both a putative receptor binding domain and a target of highly specifi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.05.024
更新日期:2016-09-01 00:00:00
abstract::Here we address the role of RIG-I and TLR3 in differential cytokine responses against Simian Virus 5 (SV5) and two distinct cytokine inducing SV5 mutants. IFN-beta and IL-6 secretion was induced by infection with P/V-CPI-, an SV5 mutant with P/V substitutions, and were reduced by either siRNA-mediated knockdown of RIG...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.014
更新日期:2010-02-05 00:00:00
abstract::The spike glycoprotein (G protein) of rabies virus (CVS strain) expressed in HeLa cells from cloned cDNA mediated membrane fusion after exposure to pHs of 6.1 or below. Chemical crosslinking showed that the rabies G protein, like the vesicular stomatitis virus (VSV) G protein, could be crosslinked to dimers and trimer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90539-n
更新日期:1991-12-01 00:00:00
abstract::NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1059
更新日期:1993-02-01 00:00:00
abstract::Unlike cell lines transformed with Polyomaviruses, transformants derived by focus formation or colony formation in agarose medium after transfer into rat fibroblast cells (FR3T3 line) of Bovine Papilloma Type 1 (BPV1) DNA were consistently observed to grow poorly in suspension and to remain highly serum dependent for ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90196-x
更新日期:1984-06-01 00:00:00
abstract::In this study, we investigated the autoimmune response in rabbit ovaries following infection with a recombinant myxoma virus expressing rabbit zona pellucida protein B (MV-ZPB). A specific IgG antibody response to ZPB was elicited in the serum of infected rabbits and the antibody strongly bound to the zona pellucida o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.10.021
更新日期:2004-01-20 00:00:00
abstract::Expression of adenosine deaminase acting on RNA1 (ADAR1) is driven by alternative promoters. Promoter PA, activated by interferon (IFN), produces transcripts that encode the inducible p150 ADAR1 protein, whereas PB specifies the constitutively expressed p110 protein. We show using Stat1(-/-), Stat2(-/-) and IRF9(-/-) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.08.001
更新日期:2015-11-01 00:00:00
abstract::West Nile virus (WNV) has become firmly established in northeastern US, reemerging every summer since its introduction into North America in 1999. To determine whether WNV overwinters locally or is reseeded annually, we examined the patterns of viral lineage persistence and replacement in Connecticut over 10 consecuti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.06.006
更新日期:2011-08-15 00:00:00
abstract::The effect of measles virus (MV) infection on mRNA expression and protein synthesis of cytokines in human malignant glioma cell lines (D-54 and U-251) was investigated. Primary MV infections led in both cell lines to the induction of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), interferon-beta (IFN-beta), and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1363
更新日期:1993-07-01 00:00:00
abstract::The X protein (pX) of hepatitis B virus (HBV) is a general transcription regulator and directly associated with the transcription machinery. pX cannot bind DNA directly but interacts with cellular factors that bind the regulatory elements. There is an accumulation of evidence concerning different activities exerted by...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1356
更新日期:1994-07-01 00:00:00
abstract::KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.10.013
更新日期:2014-01-05 00:00:00
abstract::HCV culture in vitro results in massive cell death, which suggests the presence of HCV-induced cytopathic effects. Therefore, we investigated its mechanisms and viral nucleotide sequences involved in this effect using HCV-JFH1 cell culture and a newly developed HCV plaque assay technique. The plaque assay developed cy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.09.019
更新日期:2008-02-05 00:00:00
abstract::Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90375-1
更新日期:1979-08-01 00:00:00
abstract::The function of a substantial percentage of the putative protein-coding open reading frames (ORFs) in viral genomes is unknown. As their sequence is not similar to that of proteins of known function, the function of these ORFs cannot be assigned on the basis of sequence similarity. Methods complement or in combination...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.10.020
更新日期:2005-01-05 00:00:00
abstract::H7N9 influenza A first caused human infections in early 2013 in China. Virus genetics, histories of patient exposures to poultry, and previous experimental studies suggest the source of the virus is a domestic avian species, such as chickens. In order to better understand the ecology of this H7N9 in chickens, we evalu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.01.013
更新日期:2015-03-01 00:00:00
abstract::HIV-1 variants resistant to small molecule CCR5 inhibitors such as vicriviroc (VVC) have modified Env complexes that can use both the inhibitor-bound and -free forms of the CCR5 co-receptor to enter target cells. However, entry via the inhibitor-CCR5 complex is inefficient in some, but not all, cell types, particularl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.02.044
更新日期:2009-05-10 00:00:00
abstract::Beet necrotic yellow vein virus (BNYVV) has a quadripartite plus-strand RNA genome in which the two smallest genome components, RNA 3 and 4, are not necessary for virus multiplication in leaves. Infectious transcripts of BNYVV RNA 3 and 4 have already been described (V. Ziegler-Graff, S. Bouzoubaa, I. Jupin, H. Guille...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90131-1
更新日期:1989-09-01 00:00:00
abstract::The DNAs from the granulosis viruses of Plodia interpunctella and Pieris rapae were clearly distinguishable based on restriction endonuclease fragment patterns. By sizing single- and double-digestion products approximate molecular weights of 72 x 10(2) and 75 x 10(6), respectively, were estimated for the viral genomes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90357-8
更新日期:1980-07-30 00:00:00
abstract::Callitrichid hepatitis (CH) is a highly fatal, rodent-borne zoonosis of New World primates (family Callitrichidae) caused by lymphocytic choriomeningitis virus (LCMV). It is unclear whether virulence in Callitrichidae is associated with specific genetic or phylogenetic markers of the virus as only a partial S RNA sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0909
更新日期:2001-06-05 00:00:00
abstract::Mutants of the influenza virus A/seal/Mass/1/80 (H7N7) are described which contain an insertion of 60 nucleotides in the hemagglutinin (HA) gene, derived most probably by recombination between the HA gene and the nucleoprotein gene of the same virus. The nonhomologous RNA recombination resulted in an enhanced hemagglu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1555
更新日期:1994-10-01 00:00:00
abstract::The complete nucleotide sequence of apple stem grooving virus (ASGV) genome has been determined. The genome is 6496 nucleotides in length excluding a 3'-terminal poly(A) tail and contains two overlapping open reading frames (ORFs). ORF1 begins at nucleotide position 37 and is terminated at position 6341, encoding a pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90170-t
更新日期:1992-11-01 00:00:00