Evidence for spontaneous circle formation in the replication of the satellite RNA of tobacco ringspot virus.

Abstract:

:Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not enzymically-catalyzed in vitro circularization reaction is characteristic of unit-length sTobRV RNA of the less abundant, (-) polarity. We mutated sTobRV RNA at two sites that are distant from each other in the polyribonucleotide chain. A third form of the sTobRV RNA was mutated at both sites. Multimeric forms of the one-site mutants of sTobRV(+)RNA and sTobRV(-)RNA showed, respectively, undiminished and slightly diminished self-cleavage, whereas the spontaneous circularization of each one-site-mutated, unit-length sTobRV(-)RNA was greatly reduced, compared to the reactions of wild-type sTobRV RNA and the two-site mutant. The two-site mutant and the wild-type sTobRV RNAs replicated with similar efficiency. They reduced the titer of, and severity of, symptoms induced by coinoculated tobacco ringspot virus (TobRV). When coinoculated with TobRV, neither one-site mutant increased or provided protection against TobRV. Rather, each induced a substantial accumulation of what is apparently an endogenous form of sTobRV RNA. Our results are consistent with the formation of circular sTobRV(-)RNA as an essential step in sTobRV RNA replication.

journal_name

Virology

journal_title

Virology

authors

Van Tol H,Buzayan JM,Bruening G

doi

10.1016/0042-6822(91)90005-v

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

23-30

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

180

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Immunological detection of proteins associated with the Epstein-Barr virus nuclear antigen 2A.

    abstract::The Epstein-Barr virus nuclear antigen 2A (EBNA-2A) has been strongly implicated in the EBV-mediated B-cell transformation process. Since EBNA-2A might exert this function through interaction with proteins of the infected cell, we studied the association of EBNA-2A with cellular proteins. Immunoprecipitation of EBNA-2...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1406

    authors: Grässer FA,Sauder C,Haiss P,Hille A,König S,Göttel S,Kremmer E,Leinenbach HP,Zeppezauer M,Mueller-Lantzsch N

    更新日期:1993-08-01 00:00:00

  • Guinea pig cytomegalovirus GP84 is a functional homolog of the human cytomegalovirus (HCMV) UL84 gene that can complement for the loss of UL84 in a chimeric HCMV.

    abstract::The guinea pig cytomegalovirus (GPCMV) co-linear gene and potential functional homolog of HCMV UL84 (GP84) was investigated. The GP84 gene had delayed early transcription kinetics and transient expression studies of GP84 protein (pGP84) demonstrated that it targeted the nucleus and co-localized with the viral DNA poly...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.10.028

    authors: McGregor A,Choi KY,Schleiss MR

    更新日期:2011-02-05 00:00:00

  • Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences.

    abstract::The RNA genomes of cytopathogenic bovine viral diarrhea virus (BVDV) isolates contain insertions highly homologous to cellular sequences. For two of them the insert was identified as ubiquitin coding sequence. The genome of BVDV Osloss contains exactly one ubiquitin gene monomer. In the case of BVDV CP1 the cellular i...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(91)90074-l

    authors: Meyers G,Tautz N,Dubovi EJ,Thiel HJ

    更新日期:1991-02-01 00:00:00

  • Specific restrictions in the progression of Venezuelan equine encephalitis virus-induced disease resulting from single amino acid changes in the glycoproteins.

    abstract::The pathogenesis of Venezuelan equine encephalitis virus (VEE) was examined in the mouse model using V3000, a virus derived from a molecular clone of the Trinidad donkey strain of VEE. These results were compared in parallel experiments with avirulent mutants of VEE derived by site-directed mutagenesis of the clone. A...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1022

    authors: Grieder FB,Davis NL,Aronson JF,Charles PC,Sellon DC,Suzuki K,Johnston RE

    更新日期:1995-02-01 00:00:00

  • Human herpesvirus type 8 variants circulating in Europe, Africa and North America in classic, endemic and epidemic Kaposi's sarcoma lesions during pre-AIDS and AIDS era.

    abstract::Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatro...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2009.12.005

    authors: Tornesello ML,Biryahwaho B,Downing R,Hatzakis A,Alessi E,Cusini M,Ruocco V,Katongole-Mbidde E,Loquercio G,Buonaguro L,Buonaguro FM

    更新日期:2010-03-15 00:00:00

  • The role of nucleocapsid of HIV-1 in virus assembly.

    abstract::The role of the nucleocapsid protein of HIV-1 Gag in virus assembly was investigated using Gag truncation mutants, a nucleocapsid deletion mutant, and point mutations in the nucleocapsid region of Gag, in transfected COS cells, and in stable T-cell lines. Consistent with previous investigations, a truncation containin...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9374

    authors: Dawson L,Yu XF

    更新日期:1998-11-10 00:00:00

  • Enhanced infectivity of modified bluetongue virus particles for two insect cell lines and for two Culicoides vector species.

    abstract::Previous studies (Mertens et al., Virology 157, 375-386, 1987) have shown that removal of the outer capsid layer from bluetongue virus (BTV) significantly reduces (approximately x 10(-4)) the infectivity of the resultant core particle for mammalian cells (BHK 21 cells). In contrast, the studies reported here, using a ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0153

    authors: Mertens PP,Burroughs JN,Walton A,Wellby MP,Fu H,O'Hara RS,Brookes SM,Mellor PS

    更新日期:1996-03-15 00:00:00

  • Endoplasmic reticulum export and vesicle formation of the movement protein of Chinese wheat mosaic virus are regulated by two transmembrane domains and depend on the secretory pathway.

    abstract::The 37K protein of Chinese wheat mosaic virus (CWMV) belongs to the 30K superfamily of plant virus movement proteins. CWMV 37K trans-complemented the cell-to-cell spread of a movement-defective Potato virus X. CWMV 37K fused to enhanced green fluorescent protein localized to plasmodesmata and formed endoplasmic reticu...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2012.10.024

    authors: Andika IB,Zheng S,Tan Z,Sun L,Kondo H,Zhou X,Chen J

    更新日期:2013-01-20 00:00:00

  • Regulation of human papillomavirus type 16 early gene expression in trophoblastic and cervical cells.

    abstract::We compared the outcome of different cellular and viral factors on the regulation of the HPV-16 early viral gene expression in trophoblastic and cervical cancer cells. A high variability of the long control (LCR) activity was observed, prompting us to evaluate the role of secreted factors in the control of the early g...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.12.056

    authors: Weyn C,Vanderwinden JM,Rasschaert J,Englert Y,Fontaine V

    更新日期:2011-03-30 00:00:00

  • The high levels of p53 present in adenovirus early region 1-transformed human cells do not cause up-regulation of MDM2 expression.

    abstract::The cellular protein MDM2 can bind to the tumor suppressor gene product p53 and abrogate its transcriptional activity. In addition, p53 can regulate expression of the mdm2 gene. We and others have previously shown that p53 is present at high levels in adenovirus-transformed cells which express the larger E1B protein. ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1349

    authors: Grand RJ,Lecane PS,Owen D,Grant ML,Roberts S,Levine AJ,Gallimore PH

    更新日期:1995-07-10 00:00:00

  • Properties of a new virus belonging to nodaviridae found in larval striped jack (Pseudocaranx dentex) with nervous necrosis.

    abstract::Spherical virus particles were purified from larval striped jack (Pseudocaranx dentex) with nervous necrosis. The virus consists of nonenveloped particles, about 25 nm in diameter, and contains two single-stranded, positive-sense RNA molecules with molecular weights of 1.01 x 10(6)Da (RNA 1) and 0.49 x 10(6)Da (RNA 2)...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(92)90329-n

    authors: Mori K,Nakai T,Muroga K,Arimoto M,Mushiake K,Furusawa I

    更新日期:1992-03-01 00:00:00

  • African swine fever virus EP153R open reading frame encodes a glycoprotein involved in the hemadsorption of infected cells.

    abstract::The open reading frame EP153R, located within the EcoRI E' fragment of the African swine fever (ASF) virus genome, is predicted to encode a membrane protein of 153 amino acids that presents significant homology to the N-terminal region of several CD44 molecules. EP153R contains multiple putative sites for N-glycosylat...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1999.0080

    authors: Galindo I,Almazán F,Bustos MJ,Viñuela E,Carrascosa AL

    更新日期:2000-01-20 00:00:00

  • CfMNPV blocks AcMNPV-induced apoptosis in a continuous midgut cell line.

    abstract::Morphological and molecular changes produced by Autographa californica nuclear polyhedrosis virus (AcMNPV) infection in a permissive cell line, IPLB-SF-21AE (SF-21), of Spodoptera frugiperda and a nonpermissive cell line, FPMI-CF-203 (CF-203), of Choristoneura fumiferana are described. CF-203 cells inoculated with AcM...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0411

    authors: Palli SR,Caputo GF,Sohi SS,Brownwright AJ,Ladd TR,Cook BJ,Primavera M,Arif BM,Retnakaran A

    更新日期:1996-08-01 00:00:00

  • The role of the PI3K-Akt signal transduction pathway in Autographa californica multiple nucleopolyhedrovirus infection of Spodoptera frugiperda cells.

    abstract::Many viruses activate the phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway, thereby modulating diverse downstream signaling pathways associated with antiapoptosis, proliferation, cell cycling, protein synthesis and glucose metabolism, in order to augment their replication. To date, the role of the PI3K-Akt p...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2009.06.007

    authors: Xiao W,Yang Y,Weng Q,Lin T,Yuan M,Yang K,Pang Y

    更新日期:2009-08-15 00:00:00

  • Tumorigenicity of adenovirus-transformed cells: region E1A of adenovirus 12 confers resistance to natural killer cells.

    abstract::Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12E1AB virus (containing E1A and E1B...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(85)90143-6

    authors: Sawada Y,Föhring B,Shenk TE,Raska K Jr

    更新日期:1985-12-01 00:00:00

  • Phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus adjacent to the RNA binding site affects viral RNA replication.

    abstract::Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In this paper, we demonstrate that p33 is phosphorylated in vivo and in vitro by ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2005.08.006

    authors: Shapka N,Stork J,Nagy PD

    更新日期:2005-12-05 00:00:00

  • Flanders virus replication and protein synthesis.

    abstract::The prototype strain of Flanders virus (61-7484) has been adapted to grow in Vero cell cultures and a plaque assay has been developed. Electron micrographs of infected cells revealed rod-shaped virions budding in clusters from the plasma membrane, consistent with the tentative classification of Flanders virus as a mem...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(88)90275-9

    authors: Boyd KR,Whitaker-Dowling P

    更新日期:1988-04-01 00:00:00

  • Domain structure, intracellular trafficking, and beta2-microglobulin binding of a major histocompatibility complex class I homolog encoded by molluscum contagiosum virus.

    abstract::The MC80R gene of molluscum contagiosum virus (MCV) type 1 encodes a major histocompatibility complex (MHC) class I homolog that lacks several amino-acid residues critical for peptide binding by MHC molecules, contains an unusually long N-terminal hydrophobic domain possibly derived by triplication of a signal peptide...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9390

    authors: Senkevich TG,Moss B

    更新日期:1998-10-25 00:00:00

  • Red clover necrotic mosaic virus replication proteins accumulate at the endoplasmic reticulum.

    abstract::Red clover necrotic mosaic virus (RCNMV) encodes N-terminally overlapping proteins of 27 and 88 kDa (p27 and p88) known to be required for replication. Green fluorescent protein (GFP) fusions were used to visualize the location of p27 and p88 within Nicotiana benthamiana cells. GFP:p27 fusions localized to the endopla...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2003.12.006

    authors: Turner KA,Sit TL,Callaway AS,Allen NS,Lommel SA

    更新日期:2004-03-15 00:00:00

  • In vitro mutational and inhibitory analysis of the cis-acting translational elements within the 5' untranslated region of coxsackievirus B3: potential targets for antiviral action of antisense oligomers.

    abstract::The 5' untranslated region (5'UTR) of coxsackievirus B3 (CVB3) RNA forms a highly ordered secondary structure that has been implicated in controlling initiation of viral translation by internal ribosomal entry. To test this hypothesis, synthetic bicistronic RNAs, with all or part of the 5'UTR in the intercistronic spa...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.8366

    authors: Yang D,Wilson JE,Anderson DR,Bohunek L,Cordeiro C,Kandolf R,McManus BM

    更新日期:1997-02-03 00:00:00

  • Cytoplasmic R-peptide of murine leukemia virus envelope protein negatively regulates its interaction with the cell surface receptor.

    abstract::Cytoplasmic tails of envelope (Env) glycoproteins of many retroviruses inhibit their membrane fusion activity. The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechan...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2019.04.005

    authors: Kubo Y,Izumida M,Togawa K,Zhang F,Hayashi H

    更新日期:2019-06-01 00:00:00

  • Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus.

    abstract::The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two poly...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(92)90094-6

    authors: Liu DX,Inglis SC

    更新日期:1992-01-01 00:00:00

  • Identification of the essential cysteine and histidine residues of the turnip yellow mosaic virus protease.

    abstract::The nonstructural protein expressed from ORF-206 of turnip yellow mosaic virus is proteolytically processed to produce N-terminal 150-kDa and C-terminal 70-kDa proteins. Through the use of linker insertion and deletion analyses coupled to in vitro translation, we have delimited the protease domain to residues 731-885 ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1017

    authors: Bransom KL,Dreher TW

    更新日期:1994-01-01 00:00:00

  • Sequence complementarity of sonchus yellow net virus RNA with RNA isolated from the polysomes of infected tobacco.

    abstract::Polyribosomal RNA from tobacco infected with sonchus yellow net virus (SYNV) contained sequences which hybridized to 125I-labeled SYNV RNA and which were complementary to 80 to 100% of the viral RNA genome. The poly(A)-containing RNA from polyribosomes was complementary to over 90% of the viral genome but the polyribo...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(79)90375-1

    authors: Milner JJ,Jackson AO

    更新日期:1979-08-01 00:00:00

  • Membrane binding properties of EBV gp110 C-terminal domain; evidences for structural transition in the membrane environment.

    abstract::Gp110 of Epstein-Barr virus (EBV) mainly localizes on nuclear/ER membranes and plays a role in the assembly of EBV nucleocapsid. The C-terminal tail domain (gp110 CTD) is essential for the function of gp110 and the nuclear/ER membranes localization of gp110 is ruled by its C-terminal unique nuclear localization signal...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2008.06.031

    authors: Park SJ,Seo MD,Lee SK,Lee BJ

    更新日期:2008-09-30 00:00:00

  • The three subunits of the polymerase and the nucleoprotein of influenza B virus are the minimum set of viral proteins required for expression of a model RNA template.

    abstract::The genes encoding the nucleoprotein, PB1, PB2, and PA proteins of the influenza virus strain B/Panamá/45/90 have been cloned under control of the T7 RNA polymerase promoter of plasmid pGEM-3. Transfection of the recombinant plasmids obtained into mammalian cells, which had been infected with a vaccinia virus encoding...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1997.8682

    authors: Jambrina E,Bárcena J,Uez O,Portela A

    更新日期:1997-09-01 00:00:00

  • Identification of a set of proteins (C' and C) encoded by the bicistronic P gene of the Indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral RNA polymerase.

    abstract::Previous work (C.F. Spiropoulou and S.T. Nichol, 1993, J. Virol. 67, 3103-3110) has demonstrated the existence in cells infected with the New Jersey serotype of vesicular stomatitis virus (VSV) of two small carboxy-coterminal proteins encoded by the P mRNA. These proteins have been named C' and C. We are interested in...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0202

    authors: Peluso RW,Richardson JC,Talon J,Lock M

    更新日期:1996-04-15 00:00:00

  • Select resistance-associated mutations in blood are associated with lower CSF viral loads and better neuropsychological performance.

    abstract:BACKGROUND:When antiretroviral therapy does not fully suppress HIV replication, suboptimal levels of antiretrovirals can select for antiretroviral resistant variants of HIV. These variants may exhibit reduced replication capacity and result in lower viral loads in blood. Our study evaluated whether antiretroviral resis...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2009.08.007

    authors: Hightower GK,Letendre SL,Cherner M,Gibson SA,Ellis RJ,Wolfson TJ,Gamst AC,Ignacio CC,Heaton RK,Grant I,Richman DD,Smith DM,HNRC Group.

    更新日期:2009-11-25 00:00:00

  • Internalization and trafficking mechanisms of coxsackievirus B3 in HeLa cells.

    abstract::Coxsackievirus B3 (CVB3) is nonenveloped and has a single-stranded positive-sense RNA genome. CVB3 induces myocarditis and ultimately dilated cardiomyopathy. Although there are mounting evidences of an interaction between CVB3 particles and the cellular receptors, coxsackievirus and adenovirus receptor (CAR) and decay...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2004.12.010

    authors: Chung SK,Kim JY,Kim IB,Park SI,Paek KH,Nam JH

    更新日期:2005-03-01 00:00:00

  • Pathogenesis of the slow disease of the central nervous system associated with wild mouse virus. III. Role of input virus and MCF recombinants in disease.

    abstract::Inbred mice bearing the FV-1n marker when inoculated at birth with an ecotropic murine leukemia virus (WM 1504-E) obtained from wild mice develop a progressive central nervous system (CNS) disease manifested by hindlimb paralysis and incoordination that begins by 6 to 7 weeks of age. Studies of infected SWR/J mice at ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(83)90326-4

    authors: Oldstone MB,Jensen F,Elder J,Dixon FJ,Lampert PW

    更新日期:1983-07-15 00:00:00