Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A.

abstract：:An ideal system to investigate individual determinants of the replication process of (+)-strand RNA viruses is a cell-free extract that supports viral protein and RNA synthesis in a synchronized manner. Here, we applied a translation/replication system based on cytoplasmic extracts of Nicotiana tabacum cells to Tomato...

journal_title：Virology

authors： Gursinsky T,Schulz B,Behrens SE

更新日期：2009-08-01 00:00:00

abstract：:The role of the nucleocapsid protein of HIV-1 Gag in virus assembly was investigated using Gag truncation mutants, a nucleocapsid deletion mutant, and point mutations in the nucleocapsid region of Gag, in transfected COS cells, and in stable T-cell lines. Consistent with previous investigations, a truncation containin...

journal_title：Virology

authors： Dawson L,Yu XF

更新日期：1998-11-10 00:00:00

abstract：:Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin...

journal_title：Virology

authors： Whitbeck JC,Foo CH,Ponce de Leon M,Eisenberg RJ,Cohen GH

更新日期：2009-03-15 00:00:00

abstract：:To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...

journal_title：Virology

authors： Andino Fde J,Grayfer L,Chen G,Chinchar VG,Edholm ES,Robert J

更新日期：2015-11-01 00:00:00

abstract：:Traditional virus inoculation of plants involves mechanical rubbing of leaves, whereas in nature viruses like Tomato bushy stunt virus (TBSV) are often infected via the roots. A method was adapted to compare leaf versus root inoculation of Nicotiana benthamiana and tomato with transcripts of wild-type TBSV (wtTBSV), a...

journal_title：Virology

authors： Manabayeva SA,Shamekova M,Park JW,Ding XS,Nelson RS,Hsieh YC,Omarov RT,Scholthof HB

更新日期：2013-05-10 00:00:00

abstract：:Currently, little is known about the ankyrin/F-box protein B4. Here, we report that B4R-null viruses exhibited reduced plaque size in tissue culture, and decreased ability to spread, as assessed by multiple-step growth analysis. Electron microscopy indicated that B4R-null viruses still formed mature and extracellular ...

journal_title：Virology

authors： Burles K,Irwin CR,Burton RL,Schriewer J,Evans DH,Buller RM,Barry M

更新日期：2014-05-01 00:00:00

abstract：:A non-optimal receptor-binding specificity of avian influenza viruses is believed to hamper their replication in humans; however, the magnitude of this restriction remains undefined. Here we generated recombinant viruses, R1 and R2, that differed solely by two amino acids in the receptor-binding site of their hemagglu...

journal_title：Virology

authors： Matrosovich M,Matrosovich T,Uhlendorff J,Garten W,Klenk HD

更新日期：2007-05-10 00:00:00

abstract：:Temperate coliphage P2 integrates its genome into the host chromosome upon lysogenization via a site-specific recombination event mediated by an integrase belonging to the complex family of tyrosine recombinases. The host integration site attB (BOB') is localized in the end of the cyaR gene and shares 27 nucleotides w...

journal_title：Virology

authors： Sylwan L,Frumerie C,Haggård-Ljungquist E

更新日期：2010-09-01 00:00:00

abstract：:The P68 protein kinase (referred to as P68 based on its M(r) of 68,000 in human cells) is a serine/threonine kinase induced by interferon treatment and activated by dsRNAs. The kinase is under tight controls in virus-infected cells since once activated, it phosphorylates its natural substrate eukaryotic initiation fac...

journal_title：Virology

authors： Barber GN,Tomita J,Garfinkel MS,Meurs E,Hovanessian A,Katze MG

更新日期：1992-12-01 00:00:00

abstract：:Polyomavirus (PyV) infection induces protective T-cell-independent (TI) IgM and IgG responses in T-cell-deficient (TCR beta x delta-/-) mice. In this study, we show that PyV is a TI -2 antigen: B cells with a mutated Bruton's tyrosine kinase (Xid mutants) do not respond to PyV with antibody secretion in the absence of...

journal_title：Virology

authors： Szomolanyi-Tsuda E,Brien JD,Dorgan JE,Garcea RL,Woodland RT,Welsh RM

更新日期：2001-02-15 00:00:00

abstract：:Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...

journal_title：Virology

authors： Portetelle D,Couez D,Bruck C,Kettmann R,Mammerickx M,Van der Maaten M,Brasseur R,Burny A

更新日期：1989-03-01 00:00:00

abstract：:Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a hi...

journal_title：Virology

authors： Zhang W,Bouwman KM,van Beurden SJ,Ordonez SR,van Eijk M,Haagsman HP,Verheije MH,Veldhuizen EJA

更新日期：2017-09-01 00:00:00

abstract：:Plasmids containing the E1 region of Ad12 DNA can transform certain rodent cells into oncogenic cells. To study the role of the E1a subregion in the process of oncogenic transformation, Ad12 region E1 mutants carrying deletions in the E1a region were constructed. Deletion mutants pR7 and pR8 affect only the 13 S mRNA ...

journal_title：Virology

authors： Bos JL,Jochemsen AG,Bernards R,Schrier PI,van Ormondt H,van der Eb AJ

更新日期：1983-09-01 00:00:00

abstract：:The existence and genetic make-up of most primate retroviruses was revealed by studies of bushmeat and fecal samples from unhabituated primate communities. For these, detailed data on intra- and within-species contact rates are generally missing, which makes identification of factors influencing transmission a challen...

journal_title：Virology

authors： Gogarten JF,Akoua-Koffi C,Calvignac-Spencer S,Leendertz SA,Weiss S,Couacy-Hymann E,Koné I,Peeters M,Wittig RM,Boesch C,Hahn BH,Leendertz FH

更新日期：2014-07-01 00:00:00

abstract：:Coordinated expression of viral genes in primary infection is essential for successful infection of host cells. We examined the expression profiles of Kaposi's sarcoma-associated herpesvirus (KSHV) transcripts in productive primary infection of primary human umbilical vein endothelial cells by whole-genome reverse-tra...

journal_title：Virology

authors： Yoo SM,Zhou FC,Ye FC,Pan HY,Gao SJ

更新日期：2005-12-05 00:00:00

abstract：:Recombination is a driving force for the emergence, evolution and virulence/epidemics of viruses, comprising the Enterovirus genus of the Picornaviridae family, important for human and animal health. By analyzing 2949 complete genomes/coding sequences, we provide a thorough and up-to-date overview of the genome-wide p...

journal_title：Virology

authors： Nikolaidis M,Mimouli K,Kyriakopoulou Z,Tsimpidis M,Tsakogiannis D,Markoulatos P,Amoutzias GD

更新日期：2019-01-02 00:00:00

abstract：:Chlorella viruses or chloroviruses form polysaccharide fibers on the cell wall of host Chlorella cells after infection. Such polysaccharides are either hyaluronan synthesized by virus-encoded hyaluronan synthase (HAS) or chitin synthesized by viral chitin synthase (CHS). Some chloroviruses synthesize both hyaluronan (...

journal_title：Virology

authors： Mohammed Ali AM,Kawasaki T,Yamada T

更新日期：2005-11-10 00:00:00

abstract：:The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Vir...

journal_title：Virology

authors： Meylan PR,Spina CA,Richman DD,Kornbluth RS

更新日期：1993-03-01 00:00:00

abstract：:The details of intracellular transport pathways for glycosylated proteins remain incompletely described. We previously described a mutant Rous sarcoma virus envelope glycoprotein (gp), mu 26, with an altered membrane-spanning domain that was targeted to lysosomes after traversing the trans-Golgi. This mutant protein w...

journal_title：Virology

authors： Johnston PB,Dong JY,Hunter E

更新日期：1995-01-10 00:00:00

abstract：:An antiserum was raised against a synthetic peptide corresponding to the 18 C-terminal amino acids of a putative 16K protein encoded by the 3'-terminal open reading frame of tobacco rattle virus (TRV) RNA-1. This antiserum was used to demonstrate expression of the 16K cistron in vivo. TRV-infected tobacco protoplasts ...

journal_title：Virology

authors： Angenent GC,Verbeek HB,Bol JF

更新日期：1989-04-01 00:00:00

abstract：:The human apolipoprotein B mRNA editing enzyme catalytic peptide-like 3 (APOBEC3; A3) family of proteins (A3A-H) are known to restrict various retroviruses and retroelements, but the full complement of rhesus macaque A3 proteins remains unclear. We report the isolation and characterization of the hA3A homologue from r...

journal_title：Virology

authors： Schmitt K,Guo K,Algaier M,Ruiz A,Cheng F,Qiu J,Wissing S,Santiago ML,Stephens EB

更新日期：2011-10-10 00:00:00

abstract：:The adenovirus (Ad) single-stranded DNA binding protein (DBP) is a multifunctional protein. It is thought to consist of two domains, the amino-terminal domain involved in host-range determination and the carboxyl-terminal domain functioning in DNA replication and DNA binding. We have determined the nucleotide sequence...

journal_title：Virology

authors： Vos HL,van der Lee FM,Reemst AM,van Loon AE,Sussenbach JS

更新日期：1988-03-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in con...

journal_title：Virology

authors： Kelleher CA,Paterson RK,Dreyfus DH,Streib JE,Xu JW,Takase K,Jones JF,Gelfand EW

更新日期：1995-04-20 00:00:00

abstract：:Infected cell protein (ICP) 27 is an essential herpes simplex virus type 1 (HSV-1) phosphoprotein required for optimal viral DNA and early or late gene synthesis. Three slow-migrating immunoreactive species were detected using multiple anti-ICP27 antibodies following HSV-1 infection of HEp-2 and Vero cells in the pres...

journal_title：Virology

authors： Sanfilippo CM,Lombardozzi RC,Chirimuuta FN,Blaho JA

更新日期：2004-07-01 00:00:00

abstract：:The terminal sequences of both genomic dsRNA segments of different strains of infectious bursal disease virus were determined. Analysis of the linear sequences revealed in the 5'-noncoding region of both segments the presence of a 32-nucleotide sequence with motifs conserved between segments and serotypes. The 3'-nonc...

journal_title：Virology

authors： Mundt E,Müller H

更新日期：1995-05-10 00:00:00

abstract：:The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...

journal_title：Virology

authors： Mohammadzadeh N,Follack TB,Love RP,Stewart K,Sanche S,Chelico L

更新日期：2019-01-15 00:00:00

abstract：:Multibranched peptides (SPCs) derived either from the fusion protein (gp41) sequence or from the cleavage sequence of the human immunodeficiency virus type 1 envelope were chemically synthesized and tested for their ability to inhibit both syncytium formation and HIV production in CD4+ cells. The gp41-derived SPCs had...

journal_title：Virology

authors： Sabatier JM,Mabrouk K,Moulard M,Rochat H,Van Rietschoten J,Fenouillet E

更新日期：1996-09-15 00:00:00

abstract：:Syncytia formation has been considered important for cell-to-cell spread and pathogenesis of many viruses. As a syncytium forms, individual nuclei often congregate together, allowing close contact of nuclear membranes and possibly fusion to occur. However, there is currently no reported evidence of nuclear membrane fu...

journal_title：Virology

authors： Wang W,Yang L,Huang X,Fu W,Pan D,Cai L,Ye J,Liu J,Xia N,Cheng T,Zhu H

更新日期：2017-12-01 00:00:00

abstract：:The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode on...

journal_title：Virology

authors： Rice NR,Stephens RM,Burny A,Gilden RV

更新日期：1985-04-30 00:00:00

abstract：:Retroviruses can be grouped by viral interference measurements into classes which use common cell surface receptors. We previously tested a large number of isolates of mink cell focus-inducing (MCF) murine leukemia viruses (MuLVs), and reported that all of them share a distinct receptor on NIH/3T3 cells (A. Rein, Viro...

journal_title：Virology

authors： Rein A,Schultz A

更新日期：1984-07-15 00:00:00