Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A.


:In a previous study on the replication of Kunjin virus using immunoelectron microscopy (E. G. Westaway, J. M. Mackenzie, M. T. Kenney, M. K. Jones, and A. A. Khromykh, 1997, J. Virol. 71, 6650-6661), NS1 and NS3 were found associated with double-stranded RNA (dsRNA) within vesicle packets (VP) in infected Vero cells, suggesting that these induced membrane structures may be the cytoplasmic sites of RNA replication. NS2B and NS3 (comprising the virus-encoded protease) were colocalized within distinct paracrystalline (PC) or convoluted membranes (CM), also induced in the cytoplasm, suggesting that these membranes are the sites of proteolytic cleavage. In this study we found by immunofluorescence (IF) that the small hydrophobic nonstructural proteins NS2A and NS4A were located in discrete foci in the cytoplasm of infected cells at both 16 and 24 h postinfection, partially coincident with dsRNA foci. In cryosections of infected cells at 24 h, NS2A was located by immunogold labeling primarily within VP, associated with labeled dsRNA. NS2A fused to glutathione S-transferase (GST) bound strongly to the 3' untranslated region of Kunjin RNA and also to the proposed replicase components NS3 and NS5 in cell lysates. NS4A was localized by immunogold labeling within a majority of the virus-induced membranes, including VP, CM, and PC. GST-NS4A bound weakly to the 3' untranslated region of Kunjin RNA but was bound to NS4A strongly and to most of the other viral nonstructural proteins, including NS3 and NS5. Taken together the results indicate that the flavivirus replication complex includes NS2A and NS4A in the VP in addition to the previously identified NS1 and NS3.






Mackenzie JM,Khromykh AA,Jones MK,Westaway EG




Has Abstract


1998-06-05 00:00:00
















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    abstract::An Autographa californica nuclear polyhedrosis virus gene encoding an occlusion-derived virus (ODV) envelope protein of 56 kDa was identified and sequenced. Transcription initiates from a conserved baculovirus late motif (ATAAG) with transcripts detected from 16 through 72 hr p.i. The protein is detected in infected c...


    pub_type: 杂志文章


    authors: Braunagel SC,Elton DM,Ma H,Summers MD

    更新日期:1996-03-01 00:00:00

  • Resistance and susceptibility of bovine cells expressing herpesviral glycoprotein D homologs to herpesviral infections.

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    pub_type: 杂志文章


    authors: Chase CC,Lohff C,Letchworth GJ 3rd

    更新日期:1993-05-01 00:00:00

  • Hantaan/Andes virus DNA vaccine elicits a broadly cross-reactive neutralizing antibody response in nonhuman primates.

    abstract::At least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (HFRS); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (HPS). The most prevalent and lethal hantaviruses associated with HFRS and HPS ...


    pub_type: 杂志文章


    authors: Hooper JW,Custer DM,Smith J,Wahl-Jensen V

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  • Synergism between bovine papillomavirus type 4 and the flavonoid quercetin in cell transformation in vitro.

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    pub_type: 杂志文章


    authors: Pennie WD,Campo MS

    更新日期:1992-10-01 00:00:00

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    abstract::In a plantar cyst composed of the wall of the squamous cell layer and the horny inner substance in the lower dermis, we found characteristic pathological changes, such as cytoplasmic eosinophilic inclusions and vacuolated structure, and, immunohistochemically, the papillomavirus capsid antigen. The human papillomaviru...


    pub_type: 杂志文章


    authors: Matsukura T,Iwasaki T,Kawashima M

    更新日期:1992-09-01 00:00:00

  • Pseudotyped REV/SRV retroviruses reveal restrictions to infection and host range within members of the same receptor interference group.

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    authors: Koo HM,Parthasarathi S,Ron Y,Dougherty JP

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  • Natural variants of human adenovirus type 3 provide evidence for relative genome stability across time and geographic space.

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    pub_type: 杂志文章


    authors: Mahadevan P,Seto J,Tibbetts C,Seto D

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  • Superinfection exclusion by Citrus tristeza virus does not correlate with the production of viral small RNAs.

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    pub_type: 杂志文章


    authors: Folimonova SY,Harper SJ,Leonard MT,Triplett EW,Shilts T

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  • The minute virus of mice exploits different endocytic pathways for cellular uptake.

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    pub_type: 杂志文章


    authors: Garcin PO,Panté N

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    authors: Satyanarayana T,Gowda S,Mawassi M,Albiach-Martí MR,Ayllón MA,Robertson C,Garnsey SM,Dawson WO

    更新日期:2000-12-05 00:00:00

  • Structure of the bacteriophage lambda cohesive end site: bent DNA on both sides of the site, cosN, at which terminase introduces nicks during chromosome maturation.

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    pub_type: 杂志文章


    authors: Yeo A,Kosturka LD,Feiss M

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    pub_type: 杂志文章


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  • Single-cell RNA sequencing reveals cell type-specific HPV expression in hyperplastic skin lesions.

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    pub_type: 杂志文章


    authors: Devitt K,Hanson SJ,Tuong ZK,McMeniman E,Soyer HP,Frazer IH,Lukowski SW

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    authors: Davis C,Segev-Amzaleg N,Rotem I,Mincberg M,Amir N,Sivan S,Gitelman I,Tal J

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    authors: Zhao X,Fay J,Lambkin H,Schwartz S

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  • Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica.

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    authors: Mizutani T,Sayama Y,Nakanishi A,Ochiai H,Sakai K,Wakabayashi K,Tanaka N,Miura E,Oba M,Kurane I,Saijo M,Morikawa S,Ono S

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  • Molecular studies on bromovirus capsid protein. VII. Selective packaging on BMV RNA4 by specific N-terminal arginine residuals.

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    authors: Choi YG,Rao AL

    更新日期:2000-09-15 00:00:00

  • Human herpesvirus type 8 variants circulating in Europe, Africa and North America in classic, endemic and epidemic Kaposi's sarcoma lesions during pre-AIDS and AIDS era.

    abstract::Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatro...


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    authors: Tornesello ML,Biryahwaho B,Downing R,Hatzakis A,Alessi E,Cusini M,Ruocco V,Katongole-Mbidde E,Loquercio G,Buonaguro L,Buonaguro FM

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  • Antigenic analyses of influenza virus haemagglutinins with different receptor-binding specificities.

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    authors: Daniels RS,Douglas AR,Skehel JJ,Wiley DC,Naeve CW,Webster RG,Rogers GN,Paulson JC

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  • The majority of simian immunodeficiency virus/mne circle junctions result from ligation of unintegrated viral DNA ends that are aberrant for integration.

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  • The expressed VP4 protein of bluetongue virus binds GTP and is the candidate guanylyl transferase of the virus.

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    pub_type: 杂志文章


    authors: Le Blois H,French T,Mertens PP,Burroughs JN,Roy P

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  • The RING finger domain of the varicella-zoster virus open reading frame 61 protein is required for its transregulatory functions.

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    pub_type: 杂志文章


    authors: Moriuchi H,Moriuchi M,Cohen JI

    更新日期:1994-11-15 00:00:00

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    pub_type: 杂志文章


    authors: Zhang C,Pei X,Wang Z,Jia S,Guo S,Zhang Y,Li W

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    pub_type: 杂志文章


    authors: Rajan D,Chinnadurai R,O'Keefe EL,Boyoglu-Barnum S,Todd SO,Hartert TV,Galipeau J,Anderson LJ

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    pub_type: 杂志文章


    authors: Taylor EB,Chinchar VG,Quiniou SMA,Wilson M,Bengtén E

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  • V3 sequences of paired HIV-1 isolates from blood and cerebrospinal fluid cluster according to host and show variation related to the clinical stage of disease.

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    pub_type: 杂志文章


    authors: Keys B,Karis J,Fadeel B,Valentin A,Norkrans G,Hagberg L,Chiodi F

    更新日期:1993-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Pandrea I,Onanga R,Kornfeld C,Rouquet P,Bourry O,Clifford S,Telfer PT,Abernethy K,White LT,Ngari P,Müller-Trutwin M,Roques P,Marx PA,Simon F,Apetrei C

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    pub_type: 杂志文章


    authors: Oppi C,Fiorucci G,Ferrantini M,Battistini A,Belardelli F

    更新日期:1986-04-30 00:00:00