当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A.

Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A.

Abstract：

:In a previous study on the replication of Kunjin virus using immunoelectron microscopy (E. G. Westaway, J. M. Mackenzie, M. T. Kenney, M. K. Jones, and A. A. Khromykh, 1997, J. Virol. 71, 6650-6661), NS1 and NS3 were found associated with double-stranded RNA (dsRNA) within vesicle packets (VP) in infected Vero cells, suggesting that these induced membrane structures may be the cytoplasmic sites of RNA replication. NS2B and NS3 (comprising the virus-encoded protease) were colocalized within distinct paracrystalline (PC) or convoluted membranes (CM), also induced in the cytoplasm, suggesting that these membranes are the sites of proteolytic cleavage. In this study we found by immunofluorescence (IF) that the small hydrophobic nonstructural proteins NS2A and NS4A were located in discrete foci in the cytoplasm of infected cells at both 16 and 24 h postinfection, partially coincident with dsRNA foci. In cryosections of infected cells at 24 h, NS2A was located by immunogold labeling primarily within VP, associated with labeled dsRNA. NS2A fused to glutathione S-transferase (GST) bound strongly to the 3' untranslated region of Kunjin RNA and also to the proposed replicase components NS3 and NS5 in cell lysates. NS4A was localized by immunogold labeling within a majority of the virus-induced membranes, including VP, CM, and PC. GST-NS4A bound weakly to the 3' untranslated region of Kunjin RNA but was bound to NS4A strongly and to most of the other viral nonstructural proteins, including NS3 and NS5. Taken together the results indicate that the flavivirus replication complex includes NS2A and NS4A in the VP in addition to the previously identified NS1 and NS3.

journal_name

Virology

journal_title

Virology

authors

Mackenzie JM,Khromykh AA,Jones MK,Westaway EG

doi

10.1006/viro.1998.9156

subject

Has Abstract

pub_date

1998-06-05 00:00:00

pages

203-15

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(98)99156-5

journal_volume

245

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Identification and analysis of an Autographa californica nuclear polyhedrosis virus structural protein of the occlusion-derived virus envelope: ODV-E56.

    abstract：:An Autographa californica nuclear polyhedrosis virus gene encoding an occlusion-derived virus (ODV) envelope protein of 56 kDa was identified and sequenced. Transcription initiates from a conserved baculovirus late motif (ATAAG) with transcripts detected from 16 through 72 hr p.i. The protein is detected in infected c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0097

    authors： Braunagel SC,Elton DM,Ma H,Summers MD

    更新日期：1996-03-01 00:00:00

  • Resistance and susceptibility of bovine cells expressing herpesviral glycoprotein D homologs to herpesviral infections.

    abstract：:Bovine cell lines individually expressing two related herpesviral proteins, pseudorabies virus glycoprotein 50 and herpes simplex virus type 1 glycoprotein D, were examined for their susceptibility/resistance to infection with several alphaherpesviruses. Cell lines expressing gp50 or gD-1 resisted plaque formation by ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1269

    authors： Chase CC,Lohff C,Letchworth GJ 3rd

    更新日期：1993-05-01 00:00:00

  • Hantaan/Andes virus DNA vaccine elicits a broadly cross-reactive neutralizing antibody response in nonhuman primates.

    abstract：:At least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (HFRS); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (HPS). The most prevalent and lethal hantaviruses associated with HFRS and HPS ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.11.035

    authors： Hooper JW,Custer DM,Smith J,Wahl-Jensen V

    更新日期：2006-03-30 00:00:00

  • Synergism between bovine papillomavirus type 4 and the flavonoid quercetin in cell transformation in vitro.

    abstract：:Bovine papillomavirus type 4 (BPV-4) morphologically transforms primary bovine cells in vitro only in the presence of an activated ras gene. The transformed cells are capable of anchorage-independent growth, but are not immortal and are incapable of inducing tumors in nude mice, suggesting that other events are needed...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90926-g

    authors： Pennie WD,Campo MS

    更新日期：1992-10-01 00:00:00

  • Molecular cloning of a novel human papillomavirus (type 60) from a plantar cyst with characteristic pathological changes.

    abstract：:In a plantar cyst composed of the wall of the squamous cell layer and the horny inner substance in the lower dermis, we found characteristic pathological changes, such as cytoplasmic eosinophilic inclusions and vacuolated structure, and, immunohistochemically, the papillomavirus capsid antigen. The human papillomaviru...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)91254-r

    authors： Matsukura T,Iwasaki T,Kawashima M

    更新日期：1992-09-01 00:00:00

  • Pseudotyped REV/SRV retroviruses reveal restrictions to infection and host range within members of the same receptor interference group.

    abstract：:The reticuloendotheliosis virus (REV) group of retroviruses and type D simian retroviruses (SRV) belong to the same receptor interference group. The cellular receptor for these viruses has not yet been identified. In order to study the distribution of the receptor and to identify a receptor negative cell line, vector ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1651

    authors： Koo HM,Parthasarathi S,Ron Y,Dougherty JP

    更新日期：1994-11-15 00:00:00

  • Natural variants of human adenovirus type 3 provide evidence for relative genome stability across time and geographic space.

    abstract：:Human adenovirus type 3 (HAdV-B3) has an apparently stable genome yet remains a major circulating and problematic respiratory pathogen. Comparisons of the prototype genome to genomes from three current field strains, including two isolated from epidemics, and a laboratory strain, yielded small-scale nucleotide variati...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.10.052

    authors： Mahadevan P,Seto J,Tibbetts C,Seto D

    更新日期：2010-02-05 00:00:00

  • Superinfection exclusion by Citrus tristeza virus does not correlate with the production of viral small RNAs.

    abstract：:Superinfection exclusion (SIE), a phenomenon in which a preexisting viral infection prevents a secondary infection with the same or closely related virus, has been described for different viruses, including important pathogens of humans, animals, and plants. Several mechanisms acting at various stages of the viral lif...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.08.031

    authors： Folimonova SY,Harper SJ,Leonard MT,Triplett EW,Shilts T

    更新日期：2014-11-01 00:00:00

  • The minute virus of mice exploits different endocytic pathways for cellular uptake.

    abstract：:The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epith...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.02.054

    authors： Garcin PO,Panté N

    更新日期：2015-08-01 00:00:00

  • Closterovirus encoded HSP70 homolog and p61 in addition to both coat proteins function in efficient virion assembly.

    abstract：:Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0638

    authors： Satyanarayana T,Gowda S,Mawassi M,Albiach-Martí MR,Ayllón MA,Robertson C,Garnsey SM,Dawson WO

    更新日期：2000-12-05 00:00:00

  • Structure of the bacteriophage lambda cohesive end site: bent DNA on both sides of the site, cosN, at which terminase introduces nicks during chromosome maturation.

    abstract：:Packaging of lambda DNA is mediated by the phage-encoded enzyme, terminase, which acts at a site termed cos. cos consists of cosB, the site where terminase binds lambda DNA, and cosN, the site where nicks are introduced to generate the cohesive ends of virion DNA. cos contains multiple binding sites for gpNu1, the sma...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90085-6

    authors： Yeo A,Kosturka LD,Feiss M

    更新日期：1990-01-01 00:00:00

  • Viral metagenomics in Brazilian Pekin ducks identifies two gyrovirus, including a new species, and the potentially pathogenic duck circovirus.

    abstract：:Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our under...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.05.013

    authors： Cibulski S,Weber MN,de Sales Lima FE,Lima DA,Fernandes Dos Santos H,Teixeira TF,Varela APM,Tochetto C,Mayer FQ,Roehe PM

    更新日期：2020-09-01 00:00:00

  • Single-cell RNA sequencing reveals cell type-specific HPV expression in hyperplastic skin lesions.

    abstract：:Human Papillomavirus infection is highly prevalent worldwide. While most types of HPV cause benign warts, some high-risk types are known to cause cervical cancer, as well as cancer of the oral cavity and head and neck. Persistent cutaneous HPV infection can be particularly problematic in patients with chronic immunosu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.08.007

    authors： Devitt K,Hanson SJ,Tuong ZK,McMeniman E,Soyer HP,Frazer IH,Lukowski SW

    更新日期：2019-11-01 00:00:00

  • Coxsackievirus-induced chronic inflammatory myopathy: virus variants distinguish between acute cytopathic effects and pathogenesis of chronic disease.

    abstract：:Infection with the Tucson strain of coxsackievirus B1 (CVB1T) causes the development of chronic inflammatory myopathy (CIM) and hind limb weakness in susceptible strains of mice. In this study, a panel of six plaque-purified viruses exhibiting either small or large plaque phenotypes was derived from parental CVB1T and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8592

    authors： Tam PE,Messner RP

    更新日期：1997-06-23 00:00:00

  • The P4 promoter of the parvovirus minute virus of mice is developmentally regulated in transgenic P4-LacZ mice.

    abstract：:Activation of the minute virus of mice (MVM) P4 promoter is a key step in the life cycle of the virus and is completely dependent on host transcription factors. Since transcription-factor composition varies widely in different cell types, there is the possibility that only some cell types in the host organism have the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(02)00020-x

    authors： Davis C,Segev-Amzaleg N,Rotem I,Mincberg M,Amir N,Sivan S,Gitelman I,Tal J

    更新日期：2003-02-15 00:00:00

  • Red clover necrotic mosaic virus replication proteins accumulate at the endoplasmic reticulum.

    abstract：:Red clover necrotic mosaic virus (RCNMV) encodes N-terminally overlapping proteins of 27 and 88 kDa (p27 and p88) known to be required for replication. Green fluorescent protein (GFP) fusions were used to visualize the location of p27 and p88 within Nicotiana benthamiana cells. GFP:p27 fusions localized to the endopla...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.12.006

    authors： Turner KA,Sit TL,Callaway AS,Allen NS,Lommel SA

    更新日期：2004-03-15 00:00:00

  • Identification of a 17-nucleotide splicing enhancer in HPV-16 L1 that counteracts the effect of multiple hnRNP A1-binding splicing silencers.

    abstract：:Human papillomavirus type 16 (HPV-16) infections can in rare cases persist and cause lesions that may progress to cervical cancer. Cells in the lesions are not permissive for virus production, nor are cervical cancer cells. The intracellular environment is such that it prevents production of the highly immunogenic, vi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.08.002

    authors： Zhao X,Fay J,Lambkin H,Schwartz S

    更新日期：2007-12-20 00:00:00

  • Novel DNA virus isolated from samples showing endothelial cell necrosis in the Japanese eel, Anguilla japonica.

    abstract：:Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral ge...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.12.057

    authors： Mizutani T,Sayama Y,Nakanishi A,Ochiai H,Sakai K,Wakabayashi K,Tanaka N,Miura E,Oba M,Kurane I,Saijo M,Morikawa S,Ono S

    更新日期：2011-03-30 00:00:00

  • Molecular studies on bromovirus capsid protein. VII. Selective packaging on BMV RNA4 by specific N-terminal arginine residuals.

    abstract：:An arginine-rich RNA-binding motif (ARM) found at the N-proximal region of Brome mosaic virus (BMV) coat protein (CP) adopts alpha-helical conformation and shares homology with CPs of plant and insect RNA viruses, HIV-Rev and Tat proteins, bacterial antiterminators, and ribosomal splicing factors. The ARM of BMV CP, c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0513

    authors： Choi YG,Rao AL

    更新日期：2000-09-15 00:00:00

  • Human herpesvirus type 8 variants circulating in Europe, Africa and North America in classic, endemic and epidemic Kaposi's sarcoma lesions during pre-AIDS and AIDS era.

    abstract：:Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.12.005

    authors： Tornesello ML,Biryahwaho B,Downing R,Hatzakis A,Alessi E,Cusini M,Ruocco V,Katongole-Mbidde E,Loquercio G,Buonaguro L,Buonaguro FM

    更新日期：2010-03-15 00:00:00

  • Antigenic analyses of influenza virus haemagglutinins with different receptor-binding specificities.

    abstract：:Monoclonal antibodies were used to compare the antigenicities of the haemagglutinins of two receptor binding mutants of X-31 (H3N2) influenza virus. The mutants which differed from each other in recognizing sialic acid in either alpha 2-6 linkage or alpha 2-3 linkage to galactose also differed exclusively at residue 2...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90158-2

    authors： Daniels RS,Douglas AR,Skehel JJ,Wiley DC,Naeve CW,Webster RG,Rogers GN,Paulson JC

    更新日期：1984-10-15 00:00:00

  • The majority of simian immunodeficiency virus/mne circle junctions result from ligation of unintegrated viral DNA ends that are aberrant for integration.

    abstract：:SIV/Mne circle junctions were amplified by the polymerase chain reaction (PCR) and cloned in a bacterial plasmid. Sequence analysis of clones isolated from 11 independent PCRs reveals that the start site for plus DNA synthesis is 5' ACTG. . ., and thus an asymmetric cleavage must occur during viral integration. In add...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1329

    authors： Randolph CA,Champoux JJ

    更新日期：1993-06-01 00:00:00

  • The expressed VP4 protein of bluetongue virus binds GTP and is the candidate guanylyl transferase of the virus.

    abstract：:A minor core protein, VP4, of bluetongue virus serotype 10 (BTV-10) has been synthesized in insect cells infected with a genetically manipulated recombinant baculovirus. When insect cells were coinfected by this recombinant virus and a recombinant baculovirus expressing the two major core proteins (VP3 and VP7) of the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90600-t

    authors： Le Blois H,French T,Mertens PP,Burroughs JN,Roy P

    更新日期：1992-08-01 00:00:00

  • The RING finger domain of the varicella-zoster virus open reading frame 61 protein is required for its transregulatory functions.

    abstract：:Varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein transactivates expression of VZV promoters. VZV ORF61 is functionally homologous to herpes simplex virus type 1 (HSV-1)-infected cell protein 0 (ICP0); however, amino acid sequence homology of these two proteins is limited to the RING finger domains, a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1639

    authors： Moriuchi H,Moriuchi M,Cohen JI

    更新日期：1994-11-15 00:00:00

  • The Rice stripe virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana.

    abstract：:The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis i...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.01.007

    authors： Zhang C,Pei X,Wang Z,Jia S,Guo S,Zhang Y,Li W

    更新日期：2012-04-10 00:00:00

  • Protective role of Indoleamine 2,3 dioxygenase in Respiratory Syncytial Virus associated immune response in airway epithelial cells.

    abstract：:RSV is a major cause of severe lower respiratory infection in infants and young children. With no vaccine yet available, it is important to clarify mechanisms of disease pathogenesis. Since indoleamine-2,3-dioxygenase (IDO) is an immunomodulatory enzyme and is upregulated with RSV infection, we studied it in vivo duri...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.09.007

    authors： Rajan D,Chinnadurai R,O'Keefe EL,Boyoglu-Barnum S,Todd SO,Hartert TV,Galipeau J,Anderson LJ

    更新日期：2017-12-01 00:00:00

  • Cloning and characterization of antiviral cytotoxic T lymphocytes in channel catfish, Ictalurus punctatus.

    abstract：:To determine the role of piscine anti-viral cytotoxic cells, we analyzed the response of channel catfish to Ictalurid herpesvirus 1, commonly designated channel catfish virus (CCV). Peripheral blood leukocytes (PBL) from catfish immunized with MHC-matched, CCV-infected G14D cells (G14D-CCV) showed marked lysis of G14D...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.11.014

    authors： Taylor EB,Chinchar VG,Quiniou SMA,Wilson M,Bengtén E

    更新日期：2020-01-15 00:00:00

  • V3 sequences of paired HIV-1 isolates from blood and cerebrospinal fluid cluster according to host and show variation related to the clinical stage of disease.

    abstract：:The spread of HIV-1 to the nervous system occurs early during infection in a large number of asymptomatic virus carriers. In order to address the question whether the brain is targeted by a group of HIV-1 variants with increased neurotropic properties we have obtained 20 paired isolates from the blood and cerebrospina...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1503

    authors： Keys B,Karis J,Fadeel B,Valentin A,Norkrans G,Hagberg L,Chiodi F

    更新日期：1993-10-01 00:00:00

  • High levels of SIVmnd-1 replication in chronically infected Mandrillus sphinx.

    abstract：:Viral loads were investigated in SIVmnd-1 chronically infected mandrills and the results were compared with those previously observed in other nonpathogenic natural SIV infections. Four naturally and 11 experimentally SIVmnd-1-infected mandrills from a semi-free-ranging colony were studied during the chronic phase of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.08.015

    authors： Pandrea I,Onanga R,Kornfeld C,Rouquet P,Bourry O,Clifford S,Telfer PT,Abernethy K,White LT,Ngari P,Müller-Trutwin M,Roques P,Marx PA,Simon F,Apetrei C

    更新日期：2003-12-05 00:00:00

  • Friend murine leukemia virus and spleen focus-forming virus expression in highly malignant interferon-sensitive and interferon-resistant Friend leukemia cells.

    abstract：:Analysis of expression of the Friend murine leukemia virus (F-MuLV) and of the spleen focus forming virus (SFFV) has been undertaken in highly malignant interferon (IFN)-sensitive (745) and IFN-resistant (3Cl-8) Friend leukemia cells (FLC), serially passaged intraperitoneally in DBA/2 mice. In vivo passaged 745 cells,...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90304-1

    authors： Oppi C,Fiorucci G,Ferrantini M,Battistini A,Belardelli F

    更新日期：1986-04-30 00:00:00