Analyses of mutations selected by passaging a chimeric flavivirus identify mutations that alter infectivity and reveal an interaction between the structural proteins and the nonstructural glycoprotein NS1.


:We previously described a single-cycle dengue vaccine (RepliVAX D2) engineered from a capsid (C) gene-deleted West Nile virus (WNV) expressing dengue virus serotype 2 (DENV2) prM/E genes in place of the corresponding WNV genes. That work demonstrated that adaptation of RepliVAX D2 to grow in WNV C-expressing cells resulted in acquisition of non-synonymous mutations in the DENV2 prM/E and WNV NS2A/NS3 genes. Here we demonstrate that the prM/E mutations increase the specific infectivity of chimeric virions and the NS2A/NS3 mutations independently enhance packaging. Studies with the NS2A mutant demonstrated that it was unable to produce a larger form of NS1 (NS1'), suggesting that the mutation had been selected to eliminate a ribosomal frame-shift "slippage site" in NS2A. Evaluation of a synonymous mutation at this slippage site confirmed that genomes that failed to make NS1' were packaged more efficiently than WT genomes supporting a role for NS1/NS1' in orchestrating virion assembly.






Winkelmann ER,Widman DG,Suzuki R,Mason PW




Has Abstract


2011-12-20 00:00:00
















  • IL-12p40 gene-deficient BALB/c mice exhibit lower weight loss, reduced lung pathology and decreased sensitization to allergen in response to infection with pneumonia virus of mice.

    abstract::Respiratory syncytial virus (RSV) is a major cause of bronchiolitis and pneumonia in infants and pneumonia virus of mice (PVM) causes similar disease. BALB/c mice are highly susceptible, while C57BL/6 mice are more resistant to PVM. IL-12 was significantly more up-regulated in response to PVM infection in BALB/c than ...


    pub_type: 杂志文章


    authors: Shrivastava P,Sarkar I,Atanley E,Gomis S,van Drunen Littel-van den Hurk S

    更新日期:2016-10-01 00:00:00

  • Reinitiation at the lambda DNA origin accompanies the host SOS response.

    abstract::Abnormal reinitiation of replication from lambda origins has previously been found during infection in the presence of caffeine or cis-diamminedichloroplatinum II (cis-Pt) or when lambda infects a P2 lysogen. It was further shown that the reinitiations arising from cis-Pt treatment took place during the SOS response i...


    pub_type: 杂志文章


    authors: Schnös M,Inman RB

    更新日期:1987-06-01 00:00:00

  • The molecular biology of swinepox virus. II. The infectious cycle.

    abstract::Studies based on low-stringency hybridizations of radiolabeled swinepox virus (SPV) DNA to Southern blots containing DNA of representative members of the Orthopoxvirus, Leporipoxvirus, and Avipoxvirus genera and the Entomopoxvirus subfamily have revealed no DNA homology at this level of resolution. Antigenic relatedne...


    pub_type: 杂志文章


    authors: Massung RF,Moyer RW

    更新日期:1991-01-01 00:00:00

  • Nonhomologous recombination between the hemagglutinin gene and the nucleoprotein gene of an influenza virus.

    abstract::Mutants of the influenza virus A/seal/Mass/1/80 (H7N7) are described which contain an insertion of 60 nucleotides in the hemagglutinin (HA) gene, derived most probably by recombination between the HA gene and the nucleoprotein gene of the same virus. The nonhomologous RNA recombination resulted in an enhanced hemagglu...


    pub_type: 杂志文章


    authors: Orlich M,Gottwald H,Rott R

    更新日期:1994-10-01 00:00:00

  • Molecular analysis of coxsackievirus A16 reveals a new genetic group of enteroviruses.

    abstract::Coxsackievirus A16 (CAV16) a member of the Enterovirus genus of Picornaviridae, is associated with hand-foot-and-mouth disease, a febrile papulovesicular rash of childhood. We have determined the complete nucleotide sequence of the genome of the prototype strain of CAV16 which consists of 7413 nucleotides plus the pol...


    pub_type: 杂志文章


    authors: Pöyry T,Hyypiä T,Horsnell C,Kinnunen L,Hovi T,Stanway G

    更新日期:1994-08-01 00:00:00

  • Characterization of monoclonal antibodies to the Autographa californica nuclear polyhedrosis virus.

    abstract::Mouse hybridomas which produce monoclonal antibodies to the Autographa californica nuclear polyhedrosis virus (AcNPV) have been prepared and the protein to which each is directed has been determined. The antibody produced by clones 3F3 and 5E9 specifically bound polyhedron protein of MW 32,000 when used in immunoadsor...


    pub_type: 杂志文章


    authors: Roberts PL,Naser W

    更新日期:1982-10-30 00:00:00

  • Construction and characterisation of a full-length infectious molecular clone from a fast replicating, X4-tropic HIV-1 CRF02.AG primary isolate.

    abstract::Based on our previous analysis of HIV-1 isolates from Cameroon, we constructed a full-length infectious molecular clone from a primary isolate belonging to the CRF02.AG group of recombinant viruses which dominate the HIV-epidemic in West and Central Africa. The virus derived by transfection of the proviral clone pBD6-...


    pub_type: 杂志文章


    authors: Tebit DM,Zekeng L,Kaptué L,Kräusslich HG,Herchenröder O

    更新日期:2003-09-01 00:00:00

  • Influenza virus polymerase confers independence of the cellular cap-binding factor eIF4E for viral mRNA translation.

    abstract::The influenza virus mRNAs are structurally similar to cellular mRNAs nevertheless; the virus promotes selective translation of viral mRNAs despite the inhibition of host cell protein synthesis. The infection proceeds normally upon functional impairment of eIF4E cap-binding protein, but requires functional eIF4A helica...


    pub_type: 杂志文章


    authors: Yángüez E,Rodriguez P,Goodfellow I,Nieto A

    更新日期:2012-01-20 00:00:00

  • Molecular epidemiology of betanodavirus-sequence analysis strategies and quasispecies influence outbreak source attribution.

    abstract::The quasispecies structure of nervous necrosis virus (NNV) was determined to investigate an outbreak of viral nervous necrosis disease at a barramundi (Lates calcarifer) hatchery. A traditional epidemiological investigation indicated horizontal transmission of infection between two cohorts of fish. However, variation ...


    pub_type: 杂志文章


    authors: Hick P,Gore K,Whittington R

    更新日期:2013-02-05 00:00:00

  • Genomic organization of lettuce necrotic yellows rhabdovirus.

    abstract::We have mapped the genome of lettuce necrotic yellows virus (LNYV), the type member of the genus cytorhabdovirus of the family Rhabdoviridae. We have cloned and sequenced all intergenic regions and the 3' leader and 5' trailer of the negative-sense, single-stranded RNA genome of LNYV. The LNYV genome appears to contai...


    pub_type: 杂志文章


    authors: Wetzel T,Dietzgen RG,Dale JL

    更新日期:1994-05-01 00:00:00

  • Analysis of the roles of bluetongue virus outer capsid proteins VP2 and VP5 in determination of virus serotype.

    abstract::Analyses of reassortant and parental strains of BTV serotypes 3 and 10, in serum neutralization tests, confirmed the major role of outer capsid protein VP2 in determination of virus serotype and its involvement in serum neutralization. However, a reassortant BTV strain (R70), containing protein VP5 derived from BTV 3 ...


    pub_type: 杂志文章


    authors: Mertens PP,Pedley S,Cowley J,Burroughs JN,Corteyn AH,Jeggo MH,Jennings DM,Gorman BM

    更新日期:1989-06-01 00:00:00

  • Circular dimers of a lambda DNA in infected, nonlysogenic Escherichia coli.

    abstract::Covalently closed circular dimers of phage lambda DNA have been found in Escherichia coli infected with lambda. These dimers can be formed by either the lambda Red or Int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda O and P genes or by joining of the cohesive ends. Dimers...


    pub_type: 杂志文章


    authors: Freifelder D,Baran N,Folkmanis A,Freifelder DL

    更新日期:1977-09-01 00:00:00

  • Primary structure of brome mosaic virus coat protein.

    abstract::The amino acid sequence of BMV (brome mosaic virus) coat protein is presented and compared with the sequence derived from RNA structure. Apart from removal of the N-terminal methionine residue and acetylation of the following serine residue, no post-translational modifications occur. ...


    pub_type: 杂志文章


    authors: Moosic JP,McKean DJ,Shih DS,Kaesberg P

    更新日期:1983-09-01 00:00:00

  • Characterization of the integration site of the CMV mtr in a tumor cell line.

    abstract::Previous studies have shown that a 558-bp fragment of human cytomegalovirus (CMV) DNA contained within pCM4127 and designated CMV mtr can morphologically transform rodents cells in vitro. By cotransformation with pCM4127 and a plasmid conferring G418 resistance, pSV2neo, morphologically transformed NIH3T3 cell lines w...


    pub_type: 杂志文章


    authors: Buonaguro FM,McDougall JK,Galloway DA

    更新日期:1987-01-01 00:00:00

  • Genetic variation in rotavirus serotype 4 subtypes.

    abstract::Serotype 4 rotavirus strains have been classified into two antigenic "subtypes" by a solid phase immune electron microscopy technique in which cross-absorbed animal polyclonal immune sera are used as the source of antibodies. The sequences of the gene encoding the outer capsid glycoprotein VP7 from a single serotype 4...


    pub_type: 杂志文章


    authors: Green KY,Sarasini A,Qian Y,Gerna G

    更新日期:1992-05-01 00:00:00

  • Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus.

    abstract::The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two poly...


    pub_type: 杂志文章


    authors: Liu DX,Inglis SC

    更新日期:1992-01-01 00:00:00

  • Progress and Perspectives on HIV-1 microbicide development.

    abstract::The majority of HIV-1 infections occur via sexual intercourse. Women are the most affected by the epidemic, particularly in developing countries, due to their socio-economic dependence on men and the fact that they are often victims of gender based sexual violence. Despite significant efforts that resulted in the redu...


    pub_type: 杂志文章,评审


    authors: Alexandre KB,Mufhandu HT,London GM,Chakauya E,Khati M

    更新日期:2016-10-01 00:00:00

  • Functional similarities between HIV-1 Tat and DNA sequence-specific transcriptional activators.

    abstract::The Tat regulatory protein encoded by human immunodeficiency virus type 1 (HIV-1) induces high levels of transcription from the viral long terminal repeat (LTR) promoter element after interacting with a promoter proximal RNA target sequence. In the wild-type HIV-1 LTR, this activation is facilitated by the synergistic...


    pub_type: 杂志文章


    authors: Madore SJ,Cullen BR

    更新日期:1995-02-01 00:00:00

  • Differential dependence of the infectivity of HIV-1 group O isolates on the cellular protein cyclophilin A.

    abstract::The cellular protein Cyclophilin A (Cyp A) is packaged into human immunodeficiency virus type 1 (HIV-1) particles through a specific interaction with the capsid domain of the Gag polyprotein. Inhibition of Cyp A incorporation by mutagenesis or cyclosporin treatment severely affects infectivity of all HIV-1 M subtypes ...


    pub_type: 杂志文章


    authors: Wiegers K,Kräusslich HG

    更新日期:2002-03-15 00:00:00

  • Double infection of tobacco protoplasts with brome mosaic virus and cowpea chlorotic mottle virus.

    abstract::Mixed infections of tobacco protoplasts with Cowpea chlorotic mottle virus (CCMV) and brome mosaic virus (BMV) have been studied. Fluorescent antibody staining showed that capsids of both viruses were made in doubly infected protoplasts but only infectious BMV could be recovered, i.e., BMV dominated in mixed infection...


    pub_type: 杂志文章


    authors: Watts JW,Dawson JR

    更新日期:1980-09-01 00:00:00

  • Induction of an antiviral state by interferon in the absence of elevated levels of 2,5-oligo(A) synthetase and eIF-2 kinase.

    abstract::A series of clones has been derived from an interferon-resistant murine cell line, Ltk- aprt-, and their antiviral properties have been characterized. In the parental Ltk- aprt- line interferon is unable to establish antiviral properties or to increase the levels of 2,5-oligo(A) synthetase, the 2,5-oligo(A)-activated ...


    pub_type: 杂志文章


    authors: Lewis JA

    更新日期:1988-01-01 00:00:00

  • Susceptibility to infection by the human immunodeficiency virus (HIV) correlates with T4 expression in a parental monocytoid cell line and its subclones.

    abstract::The monocytoid tumor cell line U-937 and five derived subclones were infected with the HTLV-IIIB isolate of the human immunodeficiency virus (HIV). Susceptibility to infection and sensitivity to the cytopathic effects correlated with the expression of the T4 antigen on the cell surface. On the basis of these character...


    pub_type: 杂志文章


    authors: Asjö B,Ivhed I,Gidlund M,Fuerstenberg S,Fenyö EM,Nilsson K,Wigzell H

    更新日期:1987-04-01 00:00:00

  • Homogeneity of Powassan virus populations in naturally infected Ixodes scapularis.

    abstract::Powassan virus (POWV, Flaviviridae: Flavivirus) is the sole North American member of the tick-borne encephalitis complex and consists of two distinct lineages that are maintained in ecologically discrete enzootic transmission cycles. The underlying genetic mechanisms that lead to niche partitioning in arboviruses are ...


    pub_type: 杂志文章


    authors: Brackney DE,Brown IK,Nofchissey RA,Fitzpatrick KA,Ebel GD

    更新日期:2010-07-05 00:00:00

  • Immunity to rotavirus in T cell deficient mice.

    abstract::Rotavirus infection was studied in adult nude mice (BALB/c background), alpha beta or gamma delta and alpha beta/gamma delta T cell receptor (TCR) knockout (-/-) mice (C57BL/6 and C57BL/6 x 129 backgrounds), and SCID mice (C57BL/6 background). The gamma delta TCR -/- mice cleared infection just like control mice. All ...


    pub_type: 杂志文章


    authors: Franco MA,Greenberg HB

    更新日期:1997-11-24 00:00:00

  • Antigenic variation of molecularly cloned SIVmac239 during persistent infection in a rhesus macaque.

    abstract::Eight rhesus macaques inoculated with molecularly cloned SIVmac239 developed viremia and virus-binding antibodies, but only one (macaque 2D) developed neutralizing antibodies to the virus. Viremia persisted in macaque 2D even in the presence of neutralizing antibodies. Neutralizing antibodies in the plasma collected f...


    pub_type: 杂志文章


    authors: Joag SV,Anderson MG,Clements JE,McEntee MF,Sharma DP,Adams RJ,Narayan O

    更新日期:1993-08-01 00:00:00

  • Clinical isolates of dengue virus with distinctive susceptibility to nitric oxide radical induce differential gene responses in THP-1 cells.

    abstract::In the present study, 10 clinical isolates of dengue virus were selected according to their susceptibility to the inhibitory effect of nitric oxide radical, NO. Five of them are nitric oxide-susceptible viruses while the other five are nitric oxide-resistant viruses. These isolates were investigated to identify geneti...


    pub_type: 杂志文章


    authors: Ubol S,Chareonsirisuthigul T,Kasisith J,Klungthong C

    更新日期:2008-07-05 00:00:00

  • Analysis of Hantaan virus RNA: evidence for a new genus of bunyaviridae.

    abstract::Hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family Bunyaviridae. Nucleocapsid RNA from Hantaan virions and a control bunyavirus were examined for ribonuclease A (RNase A) se...


    pub_type: 杂志文章


    authors: Schmaljohn CS,Dalrymple JM

    更新日期:1983-12-01 00:00:00

  • Influenza virus genome C4 promoter/origin attenuates its transcription and replication activity by the low polymerase recognition activity.

    abstract::A natural variation is observed at position 4 of the 3'-end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus...


    pub_type: 杂志文章


    authors: Jiang H,Zhang S,Wang Q,Wang J,Geng L,Toyoda T

    更新日期:2010-12-20 00:00:00

  • Unsupervised learning techniques reveal heterogeneity in memory CD8+ T cell differentiation following acute, chronic and latent viral infections.

    abstract::CD8+ T lymphocytes are critical for the control of gammaherpesvirus latency. To determine how memory CD8+ T cells generated during latency differ from those primed during acute or chronic viral infection, we adoptively transferred naive P14 CD8+ T cells into uninfected recipients, and examined surface proteins, cytoki...


    pub_type: 杂志文章


    authors: Liu M,Barton ES,Jennings RN,Oldenburg DG,Whirry JM,White DW,Grayson JM

    更新日期:2017-09-01 00:00:00

  • A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus.

    abstract::The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that o...


    pub_type: 杂志文章


    authors: Truong HM,Lu Z,Kutish GF,Galeota J,Osorio FA,Pattnaik AK

    更新日期:2004-08-01 00:00:00