Characterization of a novel alphaherpesvirus associated with fatal infections of domestic rabbits.

abstract：:Mixed infections of tobacco protoplasts with Cowpea chlorotic mottle virus (CCMV) and brome mosaic virus (BMV) have been studied. Fluorescent antibody staining showed that capsids of both viruses were made in doubly infected protoplasts but only infectious BMV could be recovered, i.e., BMV dominated in mixed infection...

journal_title：Virology

authors： Watts JW,Dawson JR

更新日期：1980-09-01 00:00:00

abstract：:The 5'-terminal noncoding region sequences were determined for the genome RNAs of seven strains of St. Louis encephalitis virus (SLEV) and one strain of West Nile virus (WNV) using a single synthetic cDNA primer complementary to the 5'-terminus of the coding region of a strain of WNV RNA. The 5'-terminal sequences obt...

journal_title：Virology

authors： Brinton MA,Dispoto JH

更新日期：1988-02-01 00:00:00

abstract：:A system to associate specific genome segments with viral phenotypes and to study factors influencing genome reassortment was developed for tomato spotted wilt Tospovirus (TSWV). Reassortant isolates were generated by co-inoculating a TSWV isolate, TSWV-D, with TSWV-10 or TSWV-MD. The parental origin of each genome se...

journal_title：Virology

authors： Qiu WP,Geske SM,Hickey CM,Moyer JW

更新日期：1998-04-25 00:00:00

abstract：:Ultraviolet absorption spectra and difference spectra were determined for barley stripe mosaic virus (BSMV) virions and protein and for tobacco mosaic virus (TMV) virions and protein in buffer and in sodium dodecyl sulfate (SDS). The absorbance at 260 nm of BSMV virions disrupted in SDS was 92% of that of intact virio...

journal_title：Virology

authors： Brakke MK

更新日期：1979-10-15 00:00:00

abstract：:Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CR...

journal_title：Virology

authors： Nasseri M,Wettstein FO

更新日期：1987-12-01 00:00:00

abstract：:The 682-nt satellite DNA (sat-DNA) of Tomato leaf curl virus (TLCV) depends on the helper virus for its replication. In contrast to the strict specificity that exists in each geminivirus for its cognate replication associated protein (Rep), TLCV sat-DNA can utilize Rep encoded by distinct geminiviruses. We have used a...

journal_title：Virology

authors： Lin B,Akbar Behjatnia SA,Dry IB,Randles JW,Rezaian MA

更新日期：2003-01-20 00:00:00

abstract：:The role of the matrix (MA) domain of simian immunodeficiency virus (SIV) and bovine leukaemia virus (BLV) Gag in the assembly of virus-like particles (VLP) in insect cells has been investigated. Wild-type SIV and BLV Gag assembled to form discrete VLP structures typical of many retroviruses analysed by similar system...

journal_title：Virology

authors： Kakker NK,Mikhailov MV,Jones IM,Roy P

更新日期：2002-07-20 00:00:00

abstract：:Infection with the Tucson strain of coxsackievirus B1 (CVB1T) causes the development of chronic inflammatory myopathy (CIM) and hind limb weakness in susceptible strains of mice. In this study, a panel of six plaque-purified viruses exhibiting either small or large plaque phenotypes was derived from parental CVB1T and...

journal_title：Virology

authors： Tam PE,Messner RP

更新日期：1997-06-23 00:00:00

abstract：:We have examined FIV vif function in the context of molecular clone pF34 (GenBank Accession No. M25729). Rabbit antisera directed against the translation product of the vif gene identified a 29-kDa protein in tissue culture cells infected with FIV-pF34; this protein was not present in cultures of uninfected cells. Thu...

journal_title：Virology

authors： Shacklett BL,Luciw PA

更新日期：1994-11-01 00:00:00

abstract：:In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage mat...

journal_title：Virology

authors： Behnisch W,Schmieger H

更新日期：1985-07-30 00:00:00

abstract：:A sensitive and efficient system for the functional assay of antisense oligonucleotides (oligos) was developed based on an in vitro viral assembly system. A 120-base RNA (pRNA), which indispensably participates in bacteriophage phi 29 DNA packaging, was the target for antisense action. Antisense oligos bound to pRNA, ...

journal_title：Virology

authors： Zhang C,Garver K,Guo P

更新日期：1995-08-20 00:00:00

abstract：:Packaging of lambda DNA is mediated by the phage-encoded enzyme, terminase, which acts at a site termed cos. cos consists of cosB, the site where terminase binds lambda DNA, and cosN, the site where nicks are introduced to generate the cohesive ends of virion DNA. cos contains multiple binding sites for gpNu1, the sma...

journal_title：Virology

authors： Yeo A,Kosturka LD,Feiss M

更新日期：1990-01-01 00:00:00

abstract：:Retroperitoneal fibromatosis herpesvirus (RFHV), the macaque homolog of the human rhadinovirus, Kaposi's sarcoma-associated herpesvirus (KSHV), was first identified in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS. We cloned and sequenced the ORF73 latency-associated nuclear antigen (LAN...

journal_title：Virology

authors： Burnside KL,Ryan JT,Bielefeldt-Ohmann H,Gregory Bruce A,Thouless ME,Tsai CC,Rose TM

更新日期：2006-10-10 00:00:00

abstract：:Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteritis, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing c...

journal_title：Virology

authors： Ismail TM,Yamanaka MK,Saliki JT,el-Kholy A,Mebus C,Yilma T

更新日期：1995-04-20 00:00:00

abstract：:Bombyx mori nucleopolyhedrovirus (BmNPV) orf11 (bm11) is a highly conserved gene with unknown function. It is homologous to AcMNPV orf19. In this study, a bm11 knockout virus was constructed and its role was investigated. Expression analysis indicated that bm11 is a late gene and confocal microscopy analysis demonstra...

journal_title：Virology

authors： Wang H,Xu W,Kong X,Fan Y,Wu X

更新日期：2019-01-15 00:00:00

abstract：:The yeast retrotransposon, Ty1, produces a macromolecular structure known as a virus-like particle (VLP) as an essential part of its replication cycle. The Ty1 Gag-like structural protein TYA, p1-440, alone is capable of directing assembly of the VLP. In order to determine the TYA sequences required for assembly, we h...

journal_title：Virology

authors： Brookman JL,Stott AJ,Cheeseman PJ,Adamson CS,Holmes D,Cole J,Burns NR

更新日期：1995-09-10 00:00:00

abstract：:The Crinivirus, Lettuce infectious yellows virus (LIYV) has a bipartite, positive-sense ssRNA genome. LIYV RNA 1 encodes replication-associated proteins while RNA 2 encodes proteins needed for other aspects of the LIYV life cycle. LIYV RNA 1 ORF 2 encodes P34, a trans enhancer for RNA 2 accumulation. Here we show that...

journal_title：Virology

authors： Wang J,Stewart LR,Kiss Z,Falk BW

更新日期：2010-07-20 00:00:00

abstract：:Classical swine fever virus (CSFV) is a positive-sense RNA virus with a high degree of genetic variability among isolates. High diversity is also found in virulence, with strains covering the complete spectrum from avirulent to highly virulent. The underlying genetic determinants are far from being understood. Since R...

journal_title：Virology

authors： Töpfer A,Höper D,Blome S,Beer M,Beerenwinkel N,Ruggli N,Leifer I

更新日期：2013-03-30 00:00:00

abstract：:Bluetongue virus (BTV) VP1 protein, a component of the viral RNA-directed RNA polymerase, but not the VP4 or VP6 proteins, was specifically incorporated into baculovirus expressed BTV core-like particles (composed of VP3 and VP7) and BTV virus-like particles (composed of VP2, VP3, VP5, and VP7). The VP1 protein has be...

journal_title：Virology

authors： Loudon PT,Roy P

更新日期：1991-02-01 00:00:00

abstract：:Herpes simplex virus 1 (HSV-1) is a widespread pathogen that persists for life, replicating in surface tissues and establishing latency in peripheral ganglia. Increasingly, molecular studies of latency use cultured neuron models developed using recombinant viruses such as HSV-1 GFP-US11, a derivative of strain Patton ...

journal_title：Virology

authors： Pourchet A,Copin R,Mulvey MC,Shopsin B,Mohr I,Wilson AC

更新日期：2017-12-01 00:00:00

abstract：:The V3 loop of HIV-1 gp120 is considered occluded on many primary viruses. However, virus sensitivity to neutralization by different V3 mAbs often varies, indicating that access to V3 is not restricted equally for all antibodies. Here, we have sought to gain a better understanding of these restrictions by determining ...

journal_title：Virology

authors： Pantophlet R,Wrin T,Cavacini LA,Robinson JE,Burton DR

更新日期：2008-11-25 00:00:00

abstract：:In this study, we analyzed the replication and budding sites of severe acute respiratory syndrome coronavirus (SARS-CoV) at early time points of infection. We detected cytoplasmic accumulations containing the viral nucleocapsid protein, viral RNA and the non-structural protein nsp3. Using EM techniques, we found that ...

journal_title：Virology

authors： Stertz S,Reichelt M,Spiegel M,Kuri T,Martínez-Sobrido L,García-Sastre A,Weber F,Kochs G

更新日期：2007-05-10 00:00:00

abstract：:Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...

journal_title：Virology

authors： Najjar JA,Gentsch JR,Nathanson N,Gonzalez-Scarano F

更新日期：1985-07-30 00:00:00

abstract：:Eight rhesus macaques inoculated with molecularly cloned SIVmac239 developed viremia and virus-binding antibodies, but only one (macaque 2D) developed neutralizing antibodies to the virus. Viremia persisted in macaque 2D even in the presence of neutralizing antibodies. Neutralizing antibodies in the plasma collected f...

journal_title：Virology

authors： Joag SV,Anderson MG,Clements JE,McEntee MF,Sharma DP,Adams RJ,Narayan O

更新日期：1993-08-01 00:00:00

abstract：:IL-17-producing (Th17) and regulatory T (Treg) cells have been well established in the pathogenesis of many inflammatory diseases. To assess whether Th17 and Treg were altered in acute virus-induced myocarditis (AVMC) mice, we assessed Th17/Treg functions on different levels in AVMC. It was shown that the expression o...

journal_title：Virology

authors： Xie Y,Chen R,Zhang X,Chen P,Liu X,Xie Y,Yu Y,Yang Y,Zou Y,Ge J,Chen H

更新日期：2011-12-05 00:00:00

abstract：:Neutralization epitopes of duck hepatitis B virus (DHBV) have been previously mapped within the N-terminal portion of the pre-S protein using monoclonal antibodies. However, the immune response of ducks to this region is not well characterized at the amino acid level. To this end, we have immunized adult Pekin ducks w...

journal_title：Virology

authors： Chassot S,Lambert V,Kay A,Godinot C,Trepo C,Cova L

更新日期：1994-04-01 00:00:00

abstract：:A resonance energy transfer assay of membrane fusion developed by P. S. Uster and D. W. Deamer (Biochemistry 24, 1-8 (1985)) was used in a study of influenza haemagglutinin-mediated fusion. The characteristics of fusion and haemolysis by X-31 (H3N2) virus, a number of mutants of X-31 which fuse membranes at higher pH,...

journal_title：Virology

authors： Wharton SA,Skehel JJ,Wiley DC

更新日期：1986-02-01 00:00:00

abstract：:Previous studies have shown that the transmembrane (TM) domain of the subtype B Vpu enhances virion release from cells and some studies have shown that this domain may form an oligomeric structure with properties of an ion channel. To date, no studies have been performed to assess the role of this domain in virus path...

journal_title：Virology

authors： Hout DR,Gomez ML,Pacyniak E,Gomez LM,Inbody SH,Mulcahy ER,Culley N,Pinson DM,Powers MF,Wong SW,Stephens EB

更新日期：2005-08-15 00:00:00

abstract：:Essential host cofactors in retrovirus replication bind cis-acting sequences in the 5'untranslated region (UTR). Although host RBPs are crucial to all aspects of virus biology, elucidating their roles in replication remains a challenge to the field. Here RNA affinity-coupled-proteomics generated a comprehensive, unbia...

journal_title：Virology

authors： Stake M,Singh D,Singh G,Marcela Hernandez J,Kaddis Maldonado R,Parent LJ,Boris-Lawrie K

更新日期：2015-12-01 00:00:00

abstract：:Guinea-Bissau in West Africa has the highest prevalence of human immunodeficiency virus (HIV)-2 infection in the world, but recently the HIV-1 prevalence increased rapidly with the subsequent appearance of HIV-1 and HIV-2 dual infections. Information about the genetic subtypes of HIV in the region is limited. Therefor...

journal_title：Virology

authors： Andersson S,Norrgren H,Dias F,Biberfeld G,Albert J

更新日期：1999-09-30 00:00:00