Inhibition of phage phi 29 assembly by antisense oligonucleotides targeting viral pRNA essential for DNA packaging.


:A sensitive and efficient system for the functional assay of antisense oligonucleotides (oligos) was developed based on an in vitro viral assembly system. A 120-base RNA (pRNA), which indispensably participates in bacteriophage phi 29 DNA packaging, was the target for antisense action. Antisense oligos bound to pRNA, as revealed by a slower electrophoretic mobility of pRNA/oligo complexes in comparison with native pRNA. Infectious viruses were assembled in vitro with synthetic pRNA and DNA, as well as with viral proteins produced from cloned genes. Up to 10(7) plaque-forming units per milliliter were obtained in the absence of antisense oligos, while as few as zero plaques were detected in the presence of certain antisense oligos. A 1-base mismatch greatly influenced the inhibitory effect of the antisense oligos, but this 1-based mismatch was not important when the mismatch was placed at the end of the oligo. Five oligos did not bind pRNA or inhibit the assembly of the virion, suggesting that the RNA sequences complementary to these oligos are nonessential or buried internally in the RNA. Viral assembly was strongly inhibited by antisense oligos P15 and P10, targeting either the 5'- or the 3'-end of the pRNA, respectively. Viral assembly was also strongly inhibited by oligo P6, targeting an internal region, residues 75-91, of pRNA. Oligo P6 inhibited DNA packaging activity by blocking the binding of pRNA to the procapsid, while P10 and P15 inhibited DNA packaging activity but did not block the binding of pRNA to the procapsid, suggesting that in addition to the reported internal domain for procapsid binding, pRNA contains another domain at the paired 5'/3'-ends with a yet to be defined role in DNA translocation.






Zhang C,Garver K,Guo P




Has Abstract


1995-08-20 00:00:00
















  • N-linked glycosylation of Gn (but not Gc) is important for Crimean Congo hemorrhagic fever virus glycoprotein localization and transport.

    abstract::The mature Gn glycoprotein of Crimean Congo hemorrhagic fever (CCHF) virus contains two predicted glycosylation sites (557N and 755N). Of these, N-glycans are added only at 557N, as evidenced by abrogation of Gn-glycosylation by mutation of 557N but not 755N site. Mutational block of Gn-glycosylation at 557N did not s...


    pub_type: 杂志文章


    authors: Erickson BR,Deyde V,Sanchez AJ,Vincent MJ,Nichol ST

    更新日期:2007-05-10 00:00:00

  • Effect of receptor binding domain mutations on receptor binding and transmissibility of avian influenza H5N1 viruses.

    abstract::Although H5N1 influenza viruses have been responsible for hundreds of human infections, these avian influenza viruses have not fully adapted to the human host. The lack of sustained transmission in humans may be due, in part, to their avian-like receptor preference. Here, we have introduced receptor binding domain mut...


    pub_type: 杂志文章


    authors: Maines TR,Chen LM,Van Hoeven N,Tumpey TM,Blixt O,Belser JA,Gustin KM,Pearce MB,Pappas C,Stevens J,Cox NJ,Paulson JC,Raman R,Sasisekharan R,Katz JM,Donis RO

    更新日期:2011-04-25 00:00:00

  • Intracellular factors, but not virus receptor levels, influence the age-related outcome of DHBV infection of ducks.

    abstract::Previous serological studies of experimental infection with duck hepatitis B virus (DHBV) have shown that the outcome of infection depends largely on the age of the duck at the time of inoculation. To examine the hypothesis that decreased susceptibility with increased age might be due to the loss of the virus receptor...


    pub_type: 杂志文章


    authors: Qiao M,Gowans EJ,Burrell CJ

    更新日期:1992-02-01 00:00:00

  • Analysis of the virulence modulating region of potato spindle tuber viroid (PSTVd) by site-directed mutagenesis.

    abstract::A series of nucleotide substitutions (G46----C; C47----A; C315----U; U317----C) were introduced into the virulence modulating region of the intermediate strain of potato spindle tuber viroid (PSTVd) in order to examine their effect upon viroid infectivity and pathogenicity with the presence of all four mutations resul...


    pub_type: 杂志文章


    authors: Hammond RW

    更新日期:1992-04-01 00:00:00

  • Phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus adjacent to the RNA binding site affects viral RNA replication.

    abstract::Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In this paper, we demonstrate that p33 is phosphorylated in vivo and in vitro by ...


    pub_type: 杂志文章


    authors: Shapka N,Stork J,Nagy PD

    更新日期:2005-12-05 00:00:00

  • Construction of chimeric arteriviruses reveals that the ectodomain of the major glycoprotein is not the main determinant of equine arteritis virus tropism in cell culture.

    abstract::The recent development of arterivirus full-length cDNA clones makes possible the construction of chimeric arteriviruses for fundamental and applied studies. Using an equine arteritis virus (EAV) infectious cDNA clone, we have engineered chimeras in which the ectodomains of the two major envelope proteins, the glycopro...


    pub_type: 杂志文章


    authors: Dobbe JC,van der Meer Y,Spaan WJ,Snijder EJ

    更新日期:2001-09-30 00:00:00

  • The SARS-CoV ferret model in an infection-challenge study.

    abstract::Phase I human clinical studies involving therapeutics for emerging and biodefense pathogens with low incidence, such as the severe acute respiratory syndrome coronavirus (SARS-CoV), requires at a minimum preclinical evaluation of efficacy in two well-characterized and robust animal models. Thus, a ferret SARS-CoV mode...


    pub_type: 杂志文章


    authors: Chu YK,Ali GD,Jia F,Li Q,Kelvin D,Couch RC,Harrod KS,Hutt JA,Cameron C,Weiss SR,Jonsson CB

    更新日期:2008-04-25 00:00:00

  • An anti-phosphoinositide-specific monoclonal antibody that neutralizes HIV-1 infection of human monocyte-derived macrophages.

    abstract::HIV-1 entry into cells requires the interaction of both HIV-1 envelope proteins and membrane lipids. We investigated the mechanism of neutralization of HIV-1 infection of primary monocyte-derived macrophages (MDM) by a murine monoclonal antibody (mAb) WR321. WR321 specifically binds phosphatidylinositol-4-phosphate an...


    pub_type: 杂志文章


    authors: Jobe O,Peachman KK,Matyas GR,Asher LV,Alving CR,Rao M

    更新日期:2012-09-01 00:00:00

  • Analysis of the autophosphorylation activity of transformation defective mutants of avian erythroblastosis virus.

    abstract::The v-erb B protein of avian erythroblastosis virus (AEV) possesses an associated protein kinase activity in vitro. Analysis of temperature-sensitive mutants, and nonconditional host range mutants of AEV demonstrated that there was no simple correlation between this autophosphorylation activity and the transformation ...


    pub_type: 杂志文章


    authors: Hayman MJ,Kitchener G,Knight J,McMahon J,Watson R,Beug H

    更新日期:1986-04-15 00:00:00

  • Analysis of proviruses integrated in Fli-1 and Evi-1 regions in Cas-Br-E MuLV-induced non-T-, non-B-cell leukemias.

    abstract::The DNAs of the Cas-Br-E MuLV-induced leukemias always contain somatically acquired mink cell focus-forming (MCF) recombinant proviruses. MCF recombinants could be involved during leukemogenesis at both preleukemic times and in late-stage tumors. Among the Cas-Br-E-induced non-T-, non-B-cell leukemias, viral integrati...


    pub_type: 杂志文章


    authors: Bergeron D,Poliquin L,Houde J,Barbeau B,Rassart E

    更新日期:1992-12-01 00:00:00

  • Epstein-Barr virus BRLF1 inhibits transcription of IRF3 and IRF7 and suppresses induction of interferon-beta.

    abstract::Activation of interferon regulatory factors (IRFs) 3 and 7 is essential for the induction of Type I interferons (IFN) and innate antiviral responses, and herpesviruses have evolved mechanisms to evade such responses. We previously reported that Epstein-Barr virus BZLF1, an immediate-early (IE) protein, inhibits the fu...


    pub_type: 杂志文章


    authors: Bentz GL,Liu R,Hahn AM,Shackelford J,Pagano JS

    更新日期:2010-06-20 00:00:00

  • Detection of the readthrough protein of barley yellow dwarf virus.

    abstract::The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) rais...


    pub_type: 杂志文章


    authors: Cheng SL,Domier LL,D'Arcy CJ

    更新日期:1994-08-01 00:00:00

  • A critical Sp1 element in the rhesus rhadinovirus (RRV) Rta promoter confers high-level activity that correlates with cellular permissivity for viral replication.

    abstract::KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...


    pub_type: 杂志文章


    authors: DeMaster LK,Rose TM

    更新日期:2014-01-05 00:00:00

  • Fiber genes of adenoviruses with tropism for the eye and the genital tract.

    abstract::We have characterized the fibergenes of adenovirus type 19p (Ad19p), Ad19a, and Ad37 by sequencing. The fiber genes of Ad19a and Ad37 are identical and only five amino acids differ comparing Ad19a/Ad37 with Ad19p. Based on the translated sequences we calculated the isoelectrical points (Ips) and found that the fiber k...


    pub_type: 杂志文章


    authors: Arnberg N,Mei Yf,Wadell G

    更新日期:1997-01-06 00:00:00

  • Therapeutic immunisation with COPV early genes by epithelial DNA delivery.

    abstract::Following challenge with COPV (canine oral papillomavirus), DNA plasmids encoding COPV L1, E1 or E2 protein were delivered into oral mucosal and cutaneous sites in beagles using particle-mediated immunotherapeutic delivery (PMID). Two weeks post-challenge, a priming dose of 8 microg DNA was delivered followed by a boo...


    pub_type: 杂志文章


    authors: Moore RA,Walcott S,White KL,Anderson DM,Jain S,Lloyd A,Topley P,Thomsen L,Gough GW,Stanley MA

    更新日期:2003-09-30 00:00:00

  • Lack of chicken adaptation of newly emergent Eurasian H5N8 and reassortant H5N2 high pathogenicity avian influenza viruses in the U.S. is consistent with restricted poultry outbreaks in the Pacific flyway during 2014-2015.

    abstract::In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian clade H5 highly pathogenic avian influenza (HPAI) virus, initially affecting mainly wild birds and few backyard and commercial poultry premises. To better model the outbreak, the pathogenesis and transmission dynamics of representative Eu...


    pub_type: 杂志文章


    authors: Bertran K,Swayne DE,Pantin-Jackwood MJ,Kapczynski DR,Spackman E,Suarez DL

    更新日期:2016-07-01 00:00:00

  • A novel type D simian retrovirus naturally infecting the Indian Hanuman langur (Semnopithecus entellus).

    abstract::As a simian species, the langurs are not known to harbor simian retroviruses, except for one report on a simian Type D endogenous retrovirus from the spectacled langur (Trachypithecus obscurus) from Malaysia. The present report describes for the first time natural infection of the common Hanuman langur (Semnopithecus ...


    pub_type: 杂志文章


    authors: Nandi JS,Bhavalkar-Potdar V,Tikute S,Raut CG

    更新日期:2000-11-10 00:00:00

  • Comprehensive virome analysis reveals the complexity and diversity of the viral spectrum in pediatric patients diagnosed with severe and mild hand-foot-and-mouth disease.

    abstract::The management of hand-foot-and-mouth disease(HFMD) epidemic is difficult due to the frequent emergence of non-EV71 and non-CVA16 enteroviruses and some cases testing negative for HFMD-associated causative agents. To clarify the virus spectrum of mild and severe HFMD, a comprehensive virome analysis of 238 samples was...


    pub_type: 杂志文章


    authors: Wang C,Zhou S,Xue W,Shen L,Huang W,Zhang Y,Li X,Wang J,Zhang H,Ma X

    更新日期:2018-05-01 00:00:00

  • A new hepatitis B virus variant in a chronic carrier with multiple episodes of viral reactivation and acute hepatitis.

    abstract::To examine whether there are HBV variants not yet described which cannot express HBe due to a mutation in the pre-C region, and, if they exist, whether they cause a particular course of infection and disease, we analyzed the HBV genomes of a HBs/anti-HBe positive chronic carrier who had several episodes of acute reexa...


    pub_type: 杂志文章


    authors: Raimondo G,Schneider R,Stemler M,Smedile V,Rodino G,Will H

    更新日期:1990-11-01 00:00:00

  • Epitope mapping and use of epitope-specific antisera to characterize the VP5* binding site in rotavirus SA11 NSP4.

    abstract::Rotavirus (RV) is the leading cause of infantile gastroenteritis worldwide. RV nonstructural protein 4 (NSP4), the first characterized viral enterotoxin, is a 28-kDa glycoprotein that has pleiotropic functions in RV infection and pathogenesis. NSP4 has multiple forms enabling it to perform its different functions. Dis...


    pub_type: 杂志文章


    authors: Hyser JM,Zeng CQ,Beharry Z,Palzkill T,Estes MK

    更新日期:2008-03-30 00:00:00

  • Tomato spotted wilt Tospovirus genome reassortment and genome segment-specific adaptation.

    abstract::A system to associate specific genome segments with viral phenotypes and to study factors influencing genome reassortment was developed for tomato spotted wilt Tospovirus (TSWV). Reassortant isolates were generated by co-inoculating a TSWV isolate, TSWV-D, with TSWV-10 or TSWV-MD. The parental origin of each genome se...


    pub_type: 杂志文章


    authors: Qiu WP,Geske SM,Hickey CM,Moyer JW

    更新日期:1998-04-25 00:00:00

  • Phylogenetic analysis of 49 newly derived HIV-1 group O strains: high viral diversity but no group M-like subtype structure.

    abstract::We assess the genetic relationships between 49 HIV-1 group O strains from 24 and 25 patients living in Cameroon and France, respectively. Strains were sequenced in four genomic regions: gag (p24) and three env regions (C2-V3, gp41, and for 22 C2-gp41). In each of the genomic regions analyzed, the genetic diversity amo...


    pub_type: 杂志文章


    authors: Roques P,Robertson DL,Souquière S,Damond F,Ayouba A,Farfara I,Depienne C,Nerrienet E,Dormont D,Brun-Vézinet F,Simon F,Mauclère P

    更新日期:2002-10-25 00:00:00

  • Glycoprotein D of HSV-1 is dependent on tegument protein UL16 for packaging and contains a motif that is differentially required for syncytia formation.

    abstract::Glycoprotein D (gD) of herpes simplex virus type 1 (HSV-1) plays a key role in multiple events during infection including virus entry, cell-to-cell spread, and virus-induced syncytia formation. Here, we provide evidence that an arginine/lysine cluster located at the transmembrane-cytoplasm interface of gD critically c...


    pub_type: 杂志文章


    authors: Carmichael JC,Starkey J,Zhang D,Sarfo A,Chadha P,Wills JW,Han J

    更新日期:2019-01-15 00:00:00

  • Effects of host-dependent glycosylation of hemagglutinin on receptor-binding properties on H1N1 human influenza A virus grown in MDCK cells and in embryonated eggs.

    abstract::There is growing evidence that the receptor-binding characteristics of influenza viruses are affected by the host-dependent glycosylation of viral hemagglutinin (HA). To better understand these effects, we propagated two variants of the human influenza virus USSR/90/77 (which differed by the mutation Asn131 reversible...


    pub_type: 杂志文章


    authors: Gambaryan AS,Marinina VP,Tuzikov AB,Bovin NV,Rudneva IA,Sinitsyn BV,Shilov AA,Matrosovich MN

    更新日期:1998-08-01 00:00:00

  • Alphavirus production is inhibited in neurofibromin 1-deficient cells through activated RAS signalling.

    abstract::Virus-host interactions essential for alphavirus pathogenesis are poorly understood. To address this shortcoming, we coupled retrovirus insertional mutagenesis and a cell survival selection strategy to generate clonal cell lines broadly resistant to Sindbis virus (SINV) and other alphaviruses. Resistant cells had sign...


    pub_type: 杂志文章


    authors: Kolokoltsova OA,Domina AM,Kolokoltsov AA,Davey RA,Weaver SC,Watowich SJ

    更新日期:2008-07-20 00:00:00

  • Attenuating mutations in the E2 glycoprotein gene of Venezuelan equine encephalitis virus: construction of single and multiple mutants in a full-length cDNA clone.

    abstract::Attenuated mutants of Venezuelan equine encephalitis virus (VEE) were isolated by selection for rapid penetration of cultured cells (R. E. Johnston and J. F. Smith, 1988, Virology 162, 437-443). Sequence analysis of these mutants identified candidate attenuating mutations at four loci in the VEE E2 glycoprotein gene: ...


    pub_type: 杂志文章


    authors: Davis NL,Powell N,Greenwald GF,Willis LV,Johnson BJ,Smith JF,Johnston RE

    更新日期:1991-07-01 00:00:00

  • Latent murine cytomegalovirus infection in macrophages.

    abstract::In this study we show that macrophages (Mphi) are latently infected with murine cytomegalovirus (MCMV). After clearance of acute MCMV infection, the predominant form of chronic infection in Balb mice is latency rather than persistence. Peritoneal exudate cells (PECs) from latently infected Balb mice (3-9 months postin...


    pub_type: 杂志文章


    authors: Pollock JL,Presti RM,Paetzold S,Virgin HW 4th

    更新日期:1997-01-06 00:00:00

  • Productive vs non-productive infection by cell-free varicella zoster virus of human neurons derived from embryonic stem cells is dependent upon infectious viral dose.

    abstract::Varicella Zoster virus (VZV) productively infects humans causing varicella upon primary infection and herpes zoster upon reactivation from latency in neurons. In vitro studies using cell-associated VZV infection have demonstrated productive VZV-infection, while a few recent studies of human neurons derived from stem c...


    pub_type: 杂志文章


    authors: Sloutskin A,Kinchington PR,Goldstein RS

    更新日期:2013-09-01 00:00:00

  • Synthesis of a full-length infectious cDNA clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses.

    abstract::A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35S promoter in an agroinfection vector. The biological activity o...


    pub_type: 杂志文章


    authors: Prüfer D,Wipf-Scheibel C,Richards K,Guilley H,Lecoq H,Jonard G

    更新日期:1995-12-01 00:00:00

  • Identification and functional characterization of the left origin of lytic replication of murine gammaherpesvirus 68.

    abstract::Murine gammaherpesvirus 68 (MHV-68) replicates robustly in cell culture, providing a model for studying viral genome replication during de novo infection of tumor-associated herpesviruses. We have previously identified a 1.25-kb origin of lytic replication (oriLyt) for MHV-68. To further investigate the molecular mech...


    pub_type: 杂志文章


    authors: Gong D,Qi J,Arumugaswami V,Sun R,Deng H

    更新日期:2009-05-10 00:00:00