Inhibition of phage phi 29 assembly by antisense oligonucleotides targeting viral pRNA essential for DNA packaging.


:A sensitive and efficient system for the functional assay of antisense oligonucleotides (oligos) was developed based on an in vitro viral assembly system. A 120-base RNA (pRNA), which indispensably participates in bacteriophage phi 29 DNA packaging, was the target for antisense action. Antisense oligos bound to pRNA, as revealed by a slower electrophoretic mobility of pRNA/oligo complexes in comparison with native pRNA. Infectious viruses were assembled in vitro with synthetic pRNA and DNA, as well as with viral proteins produced from cloned genes. Up to 10(7) plaque-forming units per milliliter were obtained in the absence of antisense oligos, while as few as zero plaques were detected in the presence of certain antisense oligos. A 1-base mismatch greatly influenced the inhibitory effect of the antisense oligos, but this 1-based mismatch was not important when the mismatch was placed at the end of the oligo. Five oligos did not bind pRNA or inhibit the assembly of the virion, suggesting that the RNA sequences complementary to these oligos are nonessential or buried internally in the RNA. Viral assembly was strongly inhibited by antisense oligos P15 and P10, targeting either the 5'- or the 3'-end of the pRNA, respectively. Viral assembly was also strongly inhibited by oligo P6, targeting an internal region, residues 75-91, of pRNA. Oligo P6 inhibited DNA packaging activity by blocking the binding of pRNA to the procapsid, while P10 and P15 inhibited DNA packaging activity but did not block the binding of pRNA to the procapsid, suggesting that in addition to the reported internal domain for procapsid binding, pRNA contains another domain at the paired 5'/3'-ends with a yet to be defined role in DNA translocation.






Zhang C,Garver K,Guo P




Has Abstract


1995-08-20 00:00:00
















  • Monoclonal antibodies specific for the avian sarcoma virus transforming protein pp60src.

    abstract::Monoclonal antibodies to avian sarcoma/leukosis virus-coded transforming, structural, and polymerase proteins have been developed. In this first communication, a monoclonal antibody to pp60src will be characterized. The antibody can bind to pp60src in radioimmunoassays, precipitates this antigen in protein kinase test...


    pub_type: 杂志文章


    authors: Tanaka T,Kurth R

    更新日期:1984-02-01 00:00:00

  • Increase in the relative fitness of a plant virus RNA associated with its recombinant nature.

    abstract::A recombination event at the 3' end of RNA 3 in a pseudorecombinant virus (C1C2T3) having RNAs 1 and 2 from TrK7-cucumber mosaic virus (CMV) and RNA 3 from P-tomato aspermy virus (TAV) was detected by ribonuclease protection assay (RPA). Sequence analysis of the 3' end of RNA 3 of C1C2T3 and of its parental CMV and TA...


    pub_type: 杂志文章


    authors: Fernández-Cuartero B,Burgyán J,Aranda MA,Salánki K,Moriones E,García-Arenal F

    更新日期:1994-09-01 00:00:00

  • Activation of protein kinase R by hepatitis C virus RNA-dependent RNA polymerase.

    abstract::Hepatitis C virus (HCV) was shown to activate protein kinase R (PKR), which inhibits expression of interferon (IFN) and IFN-stimulated genes by controlling the translation of newly transcribed mRNAs. However, it is unknown exactly how HCV activates PKR. To address the molecular mechanism(s) of PKR activation mediated ...


    pub_type: 杂志文章


    authors: Suzuki R,Matsuda M,Shimoike T,Watashi K,Aizaki H,Kato T,Suzuki T,Muramatsu M,Wakita T

    更新日期:2019-03-01 00:00:00

  • Characterization of multiple molecular interactions between human cytomegalovirus (HCMV) and human immunodeficiency virus type 1 (HIV-1).

    abstract::In transient gene expression assays we observed an increase in expression of the bacterial chloramphenicol acetyl-transferase (CAT) gene, under the transcriptional control of the HIV-1 LTR (pLTR-CAT), when this plasmid was cotransfected into Vero or MRC-5 cells with a plasmid containing either the HCMV immediate early...


    pub_type: 杂志文章


    authors: Rando RF,Srinivasan A,Feingold J,Gonczol E,Plotkin S

    更新日期:1990-05-01 00:00:00

  • A comparison of viruses infecting two different Chlorella-like green algae.

    abstract::Five plaque-forming viruses (Pbi viruses) of the unicellular, eukaryotic, exsymbiotic Chlorella-like green alga strain Pbi were isolated from fresh water collected in Germany. The viruses were compared to two previously characterized plaque-forming viruses (NC64A viruses) of Chlorella strain NC64A. The Pbi viruses do ...


    pub_type: 杂志文章


    authors: Reisser W,Burbank DE,Meints SM,Meints RH,Becker B,Van Etten JL

    更新日期:1988-11-01 00:00:00

  • A system for the analysis of BKV non-coding control regions: application to clinical isolates from an HIV/AIDS patient.

    abstract::The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BKV exists as archetype virus and rearranged variants, w...


    pub_type: 杂志文章


    authors: Broekema NM,Abend JR,Bennett SM,Butel JS,Vanchiere JA,Imperiale MJ

    更新日期:2010-11-25 00:00:00

  • Evidence for spontaneous circle formation in the replication of the satellite RNA of tobacco ringspot virus.

    abstract::Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...


    pub_type: 杂志文章


    authors: Van Tol H,Buzayan JM,Bruening G

    更新日期:1991-01-01 00:00:00

  • Genomic cartography of varicella-zoster virus: a complete genome-based analysis of strain variability with implications for attenuation and phenotypic differences.

    abstract::In order to gain a better perspective on the true variability of varicella-zoster virus (VZV) and to catalogue the location and number of differences, 11 new complete genome sequences were compared with those previously in the public domain (18 complete genomes in total). Three of the newly sequenced genomes were deri...


    pub_type: 杂志文章


    authors: Tyler SD,Peters GA,Grose C,Severini A,Gray MJ,Upton C,Tipples GA

    更新日期:2007-03-15 00:00:00

  • Identification of four distinct neutralizing epitopes on bluetongue virus serotype 10 using neutralizing monoclonal antibodies and neutralization-escape variants.

    abstract::Neutralizing sites on bluetongue virus serotype 10 (BTV-10) were investigated with a panel of seven neutralizing monoclonal antibodies (MAbs). Each MAb was coupled to agarose beads and tested against the other MAbs and a nonneutralizing control MAb in a competitive immueprecipitation assay. In addition, neutralization...


    pub_type: 杂志文章


    authors: Heidner HW,Rossitto PV,MacLachlan NJ

    更新日期:1990-06-01 00:00:00

  • Residues in the PB2 and PA genes contribute to the pathogenicity of avian H7N3 influenza A virus in DBA/2 mice.

    abstract::Replication and transmission of avian influenza virus in humans poses a pandemic threat. The molecular determinants that facilitate this process are not well understood. We used DBA/2 mice to identify viral factors that mediate the difference in pathogenesis between a virulent (H7N3) and a non-virulent (H7N9) avian in...


    pub_type: 杂志文章


    authors: DesRochers BL,Chen RE,Gounder AP,Pinto AK,Bricker T,Linton CN,Rogers CD,Williams GD,Webby RJ,Boon AC

    更新日期:2016-07-01 00:00:00

  • NS3 protein of hepatitis C virus regulates cyclooxygenase-2 expression through multiple signaling pathways.

    abstract::Hepatitis C virus (HCV) causes chronic hepatitis, which often results in the development of liver cirrhosis and hepatocellular carcinoma (HCC) worldwide. In this study, we demonstrated that the non-structural protein NS3 of HCV enhances cyclooxygenase-2 (COX-2) gene promoter activity, COX-2 mRNA expression, COX-2 prot...


    pub_type: 杂志文章


    authors: Lu L,Wei L,Peng G,Mu Y,Wu K,Kang L,Yan X,Zhu Y,Wu J

    更新日期:2008-02-05 00:00:00

  • Sequence-specific methylation inhibits the activity of the Epstein-Barr virus LMP 1 and BCR2 enhancer-promoter regions.

    abstract::We reported earlier that variable expression of the Epstein-Barr virus (EBV) encoded membrane protein LMP 1 in nasopharyngeal carcinoma and the host-cell-phenotype-dependent activity of the BCR2 promoter (one of the possible initiator sites for transcripts of Epstein-Barr nuclear antigens) in Burkitt's lymphoma (BL) l...


    pub_type: 杂志文章


    authors: Minarovits J,Hu LF,Minarovits-Kormuta S,Klein G,Ernberg I

    更新日期:1994-05-01 00:00:00

  • Cell lines derived from avian lymphomas exhibit two distinct phenotypes.

    abstract::Lymphoid cell lines were derived from three avian leukosis virus (ALV)-induced lymphomas. These cell lines contained proviral DNA sequences integrated upstream from the c-myc proto-oncogene, expressed increased levels of c-myc RNA, and were tumorigenic in syngeneic animals. While cell surface immunoglobulin (IgM) was ...


    pub_type: 杂志文章


    authors: Baba TW,Giroir BP,Humphries EH

    更新日期:1985-07-15 00:00:00

  • The eIF4AIII RNA helicase is a critical determinant of human cytomegalovirus replication.

    abstract::Human cytomegalovirus (HCMV) was recently shown to encode a large number of spliced mRNAs. While the nuclear export of unspliced viral transcripts has been extensively studied, the role of host mRNA export factors in HCMV mRNA trafficking remains poorly defined. We found that the eIF4AIII RNA helicase, a component of ...


    pub_type: 杂志文章


    authors: Ziehr B,Lenarcic E,Cecil C,Moorman NJ

    更新日期:2016-02-01 00:00:00

  • Deletion and duplication mutations in the C-terminal nonconserved region of Sindbis virus nsP3: effects on phosphorylation and on virus replication in vertebrate and invertebrate cells.

    abstract::Little is known concerning the function of the C-terminal nonconserved region of Sindbis virus nsP3. In this report, we created a number of in-frame deletions and duplications (from 12 to 159 residues) in this region and examined their effects on Sindbis virus replication. Sindbis RNA transcripts containing these muta...


    pub_type: 杂志文章


    authors: Lastarza MW,Grakoui A,Rice CM

    更新日期:1994-07-01 00:00:00

  • Infection, replication, and cytopathology of human papillomavirus type 31 in trophoblasts.

    abstract::Human papillomavirus (HPV) DNA is preferentially found in spontaneous abortions, specifically residing in trophoblasts, and transfected HPV-16 DNA replicates and produces progeny in 3A trophoblasts in culture. In this study 3A trophoblasts were shown to display both HPV receptors and infection by HPV-31b and HPV-6 vir...


    pub_type: 杂志文章


    authors: You H,Liu Y,Agrawal N,Prasad CK,Chiriva-Internati M,Lowery CL,Kay HH,Hermonat PL

    更新日期:2003-11-25 00:00:00

  • Glycosylation of the cauliflower mosaic virus capsid polypeptide.

    abstract::Evidence is presented for the glycosylation of the cauliflower mosaic virus (CaMV) capsid polypeptide. Fluorescent derivatives of concanavalin A (Con A) were shown to bind specifically to the dissociated CaMV capsid polypeptides in polyacrylamide gels. Con A also bound specifically to intact CaMV, so inhibiting the at...


    pub_type: 杂志文章


    authors: Du Plessis DH,Smith P

    更新日期:1981-03-01 00:00:00

  • Rhesus angiotensin converting enzyme 2 supports entry of severe acute respiratory syndrome coronavirus in Chinese macaques.

    abstract::Angiotensin converting enzyme 2 (ACE2) is the receptor that severe acute respiratory syndrome coronavirus (SARS-CoV) utilizes for target cell entry and, therefore, plays an important role in SARS pathogenesis. Since Chinese rhesus (rh) macaques do not usually develop SARS after SARS-CoV infection, it has been suggeste...


    pub_type: 杂志文章


    authors: Chen Y,Liu L,Wei Q,Zhu H,Jiang H,Tu X,Qin C,Chen Z

    更新日期:2008-11-10 00:00:00

  • HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin.

    abstract::HIV-1 Vpu antagonizes the block to particle release mediated by CD317 (BST-2/HM1.24/Tetherin) via incompletely understood mechanisms. Vpu and CD317 partially reside in cholesterol-rich lipid rafts where HIV-1 budding preferentially occurs. Here we find that lipid raft association of ectopically expressed or endogenous...


    pub_type: 杂志文章


    authors: Fritz JV,Tibroni N,Keppler OT,Fackler OT

    更新日期:2012-03-01 00:00:00

  • A convenient in vivo cap donor delivery system to investigate the cap snatching of plant bunyaviruses.

    abstract::Like their animal-infecting counterparts, plant bunyaviruses use capped RNA leaders cleaved from host cellular mRNAs to prime viral genome transcription in a process called cap-snatching, but in vivo systems to investigate the details of this process are lacking for them. Here, we report that Rice stripe tenuivirus (R...


    pub_type: 杂志文章


    authors: Lin W,Wu R,Qiu P,Jing Jin,Yang Y,Wang J,Lin Z,Zhang J,Wu Z,Du Z

    更新日期:2020-01-02 00:00:00

  • Exosomes in human semen restrict HIV-1 transmission by vaginal cells and block intravaginal replication of LP-BM5 murine AIDS virus complex.

    abstract::Exosomes are membranous extracellular nanovesicles secreted by diverse cell types. Exosomes from healthy human semen have been shown to inhibit HIV-1 replication and to impair progeny virus infectivity. In this study, we examined the ability of healthy human semen exosomes to restrict HIV-1 and LP-BM5 murine AIDS viru...


    pub_type: 杂志文章


    authors: Madison MN,Jones PH,Okeoma CM

    更新日期:2015-08-01 00:00:00

  • Interaction of HCMV UL84 with C/EBPalpha transcription factor binding sites within oriLyt is essential for lytic DNA replication.

    abstract::Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the cis-acting oriLyt region and requires six core replication proteins along with UL84 and IE2. Although UL84 is thought to be the replication initiator protein, little is known about its interaction with oriLyt. We have now performed chromatin immuno...


    pub_type: 杂志文章


    authors: Kagele D,Gao Y,Smallenburg K,Pari GS

    更新日期:2009-09-15 00:00:00

  • Construction and characterization of an infectious clone generated from Chikungunya virus SL11131 strain.

    abstract::Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell l...


    pub_type: 杂志文章


    authors: Suzuki Y,Tanaka A,Maeda Y,Emi A,Fujioka Y,Sakaguchi S,Vasudevan SG,Kobayashi T,Lim CK,Takasaki T,Wu H,Nakano T

    更新日期:2021-01-02 00:00:00

  • Antigenic analyses of influenza virus haemagglutinins with different receptor-binding specificities.

    abstract::Monoclonal antibodies were used to compare the antigenicities of the haemagglutinins of two receptor binding mutants of X-31 (H3N2) influenza virus. The mutants which differed from each other in recognizing sialic acid in either alpha 2-6 linkage or alpha 2-3 linkage to galactose also differed exclusively at residue 2...


    pub_type: 杂志文章


    authors: Daniels RS,Douglas AR,Skehel JJ,Wiley DC,Naeve CW,Webster RG,Rogers GN,Paulson JC

    更新日期:1984-10-15 00:00:00

  • Sequencing approach to analyze the role of quasispecies for classical swine fever.

    abstract::Classical swine fever virus (CSFV) is a positive-sense RNA virus with a high degree of genetic variability among isolates. High diversity is also found in virulence, with strains covering the complete spectrum from avirulent to highly virulent. The underlying genetic determinants are far from being understood. Since R...


    pub_type: 杂志文章


    authors: Töpfer A,Höper D,Blome S,Beer M,Beerenwinkel N,Ruggli N,Leifer I

    更新日期:2013-03-30 00:00:00

  • Glycoprotein C-dependent attachment of herpes simplex virus to susceptible cells leading to productive infection.

    abstract::Herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. Evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein C (gC) and one independent of that glycoprotein. Thus, whereas th...


    pub_type: 杂志文章


    authors: Campadelli-Fiume G,Stirpe D,Boscaro A,Avitabile E,Foá-Tomasi L,Barker D,Roizman B

    更新日期:1990-09-01 00:00:00

  • Effects of tumor necrosis factor on viral replication and pulmonary inflammation during acute mouse adenovirus type 1 respiratory infection.

    abstract::CD8 T cells contribute to effective clearance of mouse adenovirus type 1 (MAV-1) and to virus-induced pulmonary inflammation. We characterized effects of a CD8 T cell effector, TNF, on MAV-1 pathogenesis. TNF inhibited MAV-1 replication in vitro. TNF deficiency or immunoneutralization had no effect on lung viral loads...


    pub_type: 杂志文章


    authors: Pant K,Chandrasekaran A,Chang CJ,Vageesh A,Popkov AJ,Weinberg JB

    更新日期:2020-08-01 00:00:00

  • Location, size, and complexity of epitopes on the coat protein of beet necrotic yellow vein virus studied by means of synthetic overlapping peptides.

    abstract::Five regions on the coat protein of BNYVV which had been shown previously to be involved in the formation of continuous epitopes were further analyzed by means of synthetic overlapping peptides. It was found that at least some of these regions may encompass several overlapping epitopes (or parts thereof). Four monoclo...


    pub_type: 杂志文章


    authors: Commandeur U,Koenig R,Manteuffel R,Torrance L,Lüddecke P,Frank R

    更新日期:1994-01-01 00:00:00

  • Apoptosis correlates with immune activation in intestinal lymphoid tissue from macaques acutely infected by a highly enteropathic simian immunodeficiency virus, SIVsmmPBj14.

    abstract::The PBj14 isolate of simian immunodeficiency virus, SIVsmmPBj14, induces an acutely lethal disease in experimentally inoculated pigtailed macaques, that is characterized by severe enteropathy and extensive immune activation, particularly within gut-associated lymphoid tissue (GALT). Experiments were conducted to deter...


    pub_type: 杂志文章


    authors: Gummuluru S,Novembre FJ,Lewis M,Gelbard HA,Dewhurst S

    更新日期:1996-11-01 00:00:00

  • Generation and maintenance of Listeria-specific CD8+ T cell responses in perforin-deficient mice chronically infected with LCMV.

    abstract::Disruption of the perforin gene results in primary immunodeficiency and an increased susceptibility to opportunistic pathogens. Perforin-deficient (PKO) mice fail to clear primary lymphocytic choriomeningitis virus (LCMV) Armstrong, resulting in persistent infection and functional exhaustion of virus-specific CD8+ T c...


    pub_type: 杂志文章


    authors: Humphreys TD,Khanolkar A,Badovinac VP,Harty JT

    更新日期:2008-01-20 00:00:00