Cloning and expression of the nucleoprotein of peste des petits ruminants virus in baculovirus for use in serological diagnosis.

abstract：:A procedure was developed for the purification of the pea enation mosaic virus (PEMV) using differential and rate zonal centrifugation in sucrose density gradients. Infectivity and serological data indicated that the highest virus concentration occurs in infected peas (Pisum sativum) 10-12 days after mechanical inocul...

journal_title：Virology

authors： Izadpanah K,Shepherd RJ

更新日期：1966-03-01 00:00:00

abstract：:The response to influenza virus (IAV) infection and severity of disease is highly variable in humans. We hypothesized that one factor contributing to this variability is the presence of specific respiratory tract (RT) microbes. One such microbe is Streptococcus pneumoniae (Sp) that is carried asymptomatically in the R...

journal_title：Virology

authors： Wolf AI,Strauman MC,Mozdzanowska K,Williams KL,Osborne LC,Shen H,Liu Q,Garlick D,Artis D,Hensley SE,Caton AJ,Weiser JN,Erikson J

更新日期：2014-08-01 00:00:00

abstract：:Infection of rhesus macaques with attenuated SIVmac is, at present, the only strategy which confers significant protection from challenge with wild-type SIVmac grown in monkey PBMC. However, initial results suggest that the protective mechanism does not develop until late after "vaccination" (approx 10 months). As par...

journal_title：Virology

authors： Norley S,Beer B,Binninger-Schinzel D,Cosma C,Kurth R

更新日期：1996-05-01 00:00:00

abstract：:The prototype strain of Flanders virus (61-7484) has been adapted to grow in Vero cell cultures and a plaque assay has been developed. Electron micrographs of infected cells revealed rod-shaped virions budding in clusters from the plasma membrane, consistent with the tentative classification of Flanders virus as a mem...

journal_title：Virology

authors： Boyd KR,Whitaker-Dowling P

更新日期：1988-04-01 00:00:00

abstract：:The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...

journal_title：Virology

authors： Rota PA,Maes RK,Ruyechan WT

更新日期：1986-10-15 00:00:00

abstract：:Equine influenza is caused by two serotypes of type A influenza virus, EIV-A1 and EIV-A2. The complete nucleotide sequence of the neuraminidase (NA) genes of both the A1 (N7 subtype) and A2 (N8 subtype) serotype has been determined following cloning of full-length viral NA cDNAs into pBR322. Analysis of the deduced am...

journal_title：Virology

authors： Dale B,Brown R,Miller J,White RT,Air GM,Cordell B

更新日期：1986-12-01 00:00:00

abstract：:To determine if sequence variations observed in cosmopolitan HTLV-I interfered with viral recognition by neutralizing antibodies, we evaluated the neutralization potential of sera from persons infected by HTLV-I of this clade selected for amino acid changes in their eny glycoproteins. Each serum was used to neutralize...

journal_title：Virology

authors： Blanchard S,Astier-Gin T,Moynet D,Edouard E,Guillemain B

更新日期：1998-05-25 00:00:00

abstract：:Like human adenovirus type 5 (HAV5), bovine adenovirus type 3 (BAV3) early region 1 (E1) consists of E1A and E1B transcriptional units. In order to characterize BAV3 E1 proteins and to isolate a cell line of bovine origin that expresses BAV3 E1, polyclonal antibodies specific to E1A, E1B-157R, and E1B-420R were raised...

journal_title：Virology

authors： van Olphen AL,Tikoo SK,Mittal SK

更新日期：2002-03-30 00:00:00

abstract：:The v-erb B protein of avian erythroblastosis virus (AEV) possesses an associated protein kinase activity in vitro. Analysis of temperature-sensitive mutants, and nonconditional host range mutants of AEV demonstrated that there was no simple correlation between this autophosphorylation activity and the transformation ...

journal_title：Virology

authors： Hayman MJ,Kitchener G,Knight J,McMahon J,Watson R,Beug H

更新日期：1986-04-15 00:00:00

abstract：:Simian virus (SV40) T antigen shares many characteristics with adenovirus E1A which is known to induce apoptosis. To verify the potential of SV40 T antigen-mediated apoptosis, we stably expressed T antigen in immortalized human epithelial cells (Z172 and HaCaT). We found that SV40 T antigen could directly cause apopto...

journal_title：Virology

authors： Chen SL,Tsao YP,Chen YL,Huang SJ,Chang JL,Wu SF

更新日期：1998-05-10 00:00:00

abstract：:Infectious bronchitis virus (IBV) infection and associated illness may be dramatically modified by passive transfer of immune T lymphocytes. Lymphocytes collected 10 days postinfection were transferred to naive chicks before challenge with virus. As determined by respiratory illness and viral load, transfer of syngene...

journal_title：Virology

authors： Seo SH,Pei J,Briles WE,Dzielawa J,Collisson EW

更新日期：2000-03-30 00:00:00

abstract：:Rabies continues to poses serious threats to the public health in many countries. The development of novel inexpensive, safe and effective vaccines has become a high priority for rabies control worldwide. We previously generated a novel recombinant rabies vaccine by cloning rabies virus glycoprotein into a chimpanzee ...

journal_title：Virology

authors： Wang X,Fang Z,Xiong J,Yang K,Chi Y,Tang X,Ma L,Zhang R,Deng F,Lan K,Zhou D

更新日期：2019-10-01 00:00:00

abstract：:Antigenic variation among influenza B viruses is different from that of influenza A in several ways. Antigenic shift has not been observed, distinct antigenic variants of influenza B cocirculate, and antigenically similar viruses have been isolated many years apart. To study the mechanism of antigenic drift in influen...

journal_title：Virology

authors： Hovanec DL,Air GM

更新日期：1984-12-01 00:00:00

abstract：:Recent reports indicated that vaccinia virus late mRNAs contain a unique 5' poly(A) leader sequence and that the in vitro translation of these mRNAs may be relatively cap-independent. These observations led us to examine the possibility that the mechanism of inhibition of host protein synthesis by vaccinia virus, like...

journal_title：Virology

authors： Schnierle BS,Moss B

更新日期：1992-06-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the cis-acting oriLyt region and requires six core replication proteins along with UL84 and IE2. Although UL84 is thought to be the replication initiator protein, little is known about its interaction with oriLyt. We have now performed chromatin immuno...

journal_title：Virology

authors： Kagele D,Gao Y,Smallenburg K,Pari GS

更新日期：2009-09-15 00:00:00

abstract：:The simian hemorrhagic fever virus (SHFV) genome differs from those of other members of the family Arterivirus in encoding two adjacent sets of four minor structural protein open reading frames (ORFs). A stable, full-length, infectious SHFV-LVR cDNA clone was constructed. Virus produced from this clone had replication...

journal_title：Virology

authors： Vatter HA,Di H,Donaldson EF,Baric RS,Brinton MA

更新日期：2014-08-01 00:00:00

abstract：:In previous studies, a mutant (cp-RSV) of the RSV A2 strain derived from 52 serial cold passages in bovine embryonic tissue culture was highly attenuated in seropositive adults and children but caused upper respiratory tract disease in seronegative infants. We investigated the genetic basis for this attenuation phenot...

journal_title：Virology

authors： Connors M,Crowe JE Jr,Firestone CY,Murphy BR,Collins PL

更新日期：1995-04-20 00:00:00

abstract：:Feline foamy virus (FeFV) belongs to the group of spumaretroviruses that contain in addition to gag, pol, and env accessory genes collectively called bel genes. Primate FVs have been shown to utilize internal promoters in addition to the 5' LTR promoters. In contrast to other known retroviruses, the FV pol genes are e...

journal_title：Virology

authors： Bodem J,Löchelt M,Delius H,Flügel RM

更新日期：1998-05-10 00:00:00

abstract：:Recently, cases of severe respiratory illness in military and civilian populations have been associated with a new genomic variant of adenovirus (Ad) serotype 14, designated Ad14a. Compared to the Ad14 reference strain (de Wit), this new virus had a deletion of two amino acid residues in the fiber protein knob. Here w...

journal_title：Virology

authors： Wang H,Tuve S,Erdman DD,Lieber A

更新日期：2009-05-10 00:00:00

abstract：:After infection with mumps virus, cellular ribonucleic acid synthesis of a murine lymphoma cell line, EL4, was appreciably depressed. The inactivation of viral infectivity by ultraviolet irradiation or the treatment of cells with mouse interferon did not abolish the inhibiting effect, suggesting that virus replication...

journal_title：Virology

authors： Yamada A,Tsurudome M,Hishiyama M,Ito Y

更新日期：1984-06-01 00:00:00

abstract：:Influenza A H1N2 viruses, which emerged during 2001, are genetic reassortants between H1N1 and H3N2 subtype viruses which have cocirculated in the human population since 1977. They possess a H1 hemagglutinin antigenically and genetically similar to contemporary A/New Caledonia/20/99 (H1N1)-like viruses and seven genes...

journal_title：Virology

authors： Gregory V,Bennett M,Orkhan MH,Al Hajjar S,Varsano N,Mendelson E,Zambon M,Ellis J,Hay A,Lin YP

更新日期：2002-08-15 00:00:00

abstract：:The virulence of herpes simplex virus-1 (HSV-1) strains by the intraperitoneal (ip) route of injection in mice depends on the presence of an intact sequence in the HpaI DNA fragment P within coordinates 0.762 to 0.787. Deletion of the HpaI-P region (e.g., strain HFEM) abrogates the ability of the virus to infect mice ...

journal_title：Virology

authors： Becker Y,Hadar J,Tabor E,Ben-Hur T,Raibstein I,Rösen A,Darai G

更新日期：1986-03-01 00:00:00

abstract：:We have analyzed the limiting factors involved in the induction of human immunodeficiency virus type 1 (HIV-1) provirus expression by tumor necrosis factor-alpha (TNF-alpha), phorbol-12-myristate-13-acetate (PMA), and bryostatin-1 in T-cells (ACH-2) and monocytes (U1). We have demonstrated that, while there is a corre...

journal_title：Virology

authors： Vlach J,Pitha PM

更新日期：1992-03-01 00:00:00

abstract：:Lipid binding properties which are dependent on exposure to acid pH are an important mechanism for the cellular entry pathway for some enveloped viruses and for other macromolecules. Cell-to-cell fusion can be used to demonstrate this function. La Crosse virus, a member of the family Bunyaviridae, fused BHK-21 cells f...

journal_title：Virology

authors： Gonzalez-Scarano F,Pobjecky N,Nathanson N

更新日期：1984-01-15 00:00:00

abstract：:The M128L myxoma virus gene expresses a five-membrane spanning cell surface protein with significant amino acid homology to the cellular CD47 proteins. CD47, also called integrin-associated protein (IAP), is associated with the modulation of leukocyte adhesion, motility, activation, and phagocytosis. Creation of an M1...

journal_title：Virology

authors： Cameron CM,Barrett JW,Mann M,Lucas A,McFadden G

更新日期：2005-06-20 00:00:00

abstract：:The role of the matrix (MA) domain of simian immunodeficiency virus (SIV) and bovine leukaemia virus (BLV) Gag in the assembly of virus-like particles (VLP) in insect cells has been investigated. Wild-type SIV and BLV Gag assembled to form discrete VLP structures typical of many retroviruses analysed by similar system...

journal_title：Virology

authors： Kakker NK,Mikhailov MV,Jones IM,Roy P

更新日期：2002-07-20 00:00:00

abstract：:Vaccinia virus (VV) encodes a 37-kDa envelope protein (p37) that is palmitylated on cysteine residues 185186 of the 372-amino acid protein. We have previously reported on a loosely conserved consensus motif. Further analysis has identified a conserved consensus sequence, Hydro*AAC(C)A (Hydro* represents a hydrophobic ...

journal_title：Virology

authors： Hansen SG,Grosenbach DW,Hruby DE

更新日期：1999-02-01 00:00:00

abstract：:The structure and function of the equine herpesvirus type 1 (EHV-1) UL34 homologous protein were characterized. A UL34 protein-specific antiserum reacted with an M(r)28,000 protein that could not be detected in purified extracellular virions. Confocal laser scanning microscopy demonstrated that UL34 reactivity mainly ...

journal_title：Virology

authors： Neubauer A,Rudolph J,Brandmüller C,Just FT,Osterrieder N

更新日期：2002-09-01 00:00:00

abstract：:Pre-exposure prophylaxis (PrEP) strategies utilizing anti-retroviral drugs show considerable promise for HIV prevention. However there is insufficient pharmacokinetic (PK) data on drug concentrations required for protection at the relevant mucosal tissues where the infection is initiated. Here we evaluated the utility...

journal_title：Virology

authors： Veselinovic M,Yang KH,LeCureux J,Sykes C,Remling-Mulder L,Kashuba ADM,Akkina R

更新日期：2014-09-01 00:00:00

abstract：:Homologous, subgenomic fragments of the viral LCR and E6/E7 transforming genes of HPV-18 and HPV-16 were amplified from several primary cervical, penile, and vulvar tumors and cloned into a pUC-18-derived vector. When assayed by a quantitative transformation assay using primary human keratinocytes, the subgenomic regi...

journal_title：Virology

authors： Villa LL,Schlegel R

更新日期：1991-03-01 00:00:00