Abstract:
:A procedure was developed for the purification of the pea enation mosaic virus (PEMV) using differential and rate zonal centrifugation in sucrose density gradients. Infectivity and serological data indicated that the highest virus concentration occurs in infected peas (Pisum sativum) 10-12 days after mechanical inoculation when the plants are grown at 16-22 degrees . Tests with several buffer salts at various concentrations and pH values showed that 0.1-0.3 M acetate, pH 6.0, was the most suitable for extraction of virus and maintaining its infectivity. Approximately 0.3 mg of virus could be obtained per gram of infected plant material. Infectivity was found to be associated with two nucleoprotein components with sedimentation coefficients of 106 +/- 2 and 122 +/- 1.5 S, which separated during density gradient centrifugation but which were not present in similarly treated healthy tissue. The slower sedimenting component appeared to be more infectious than the bulk of the virus material, which sedimented somewhat more rapidly. Both components were composed of small polyhedral particles approximately 36 micron in diameter as revealed by electron microscopy. In addition to the two infectious components, one other which was antigenically incomplete and of low molecular weight occurs in freshly extracted sap and the supernatant of ultracentrifuged homogenates of infected peas.
journal_name
Virologyjournal_title
Virologyauthors
Izadpanah K,Shepherd RJdoi
10.1016/0042-6822(66)90060-2subject
Has Abstractpub_date
1966-03-01 00:00:00pages
463-76issue
3eissn
0042-6822issn
1096-0341pii
0042-6822(66)90060-2journal_volume
28pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Tobacco plants transformed with the P1 and P2 replicase genes of alfalfa mosaic virus (AIMV) have been shown to produce functional replicase proteins, permitting their infection with AIMV inocula lacking the genome segments encoding P1 and P2, respectively. To see whether expression of a mutant P2 protein would interf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1106
更新日期:1995-03-10 00:00:00
abstract::The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of int...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90494-c
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND:When antiretroviral therapy does not fully suppress HIV replication, suboptimal levels of antiretrovirals can select for antiretroviral resistant variants of HIV. These variants may exhibit reduced replication capacity and result in lower viral loads in blood. Our study evaluated whether antiretroviral resis...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.007
更新日期:2009-11-25 00:00:00
abstract::Resting peripheral blood mononuclear cells (PBMC) or purified T-cells can be induced to proliferate when cocultured in vitro with fixed HTLV-infected T-cells. This process of HTLV-dependent cellular activation and induction of proliferation has been considered distinctive because of an apparent independence from conve...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0389
更新日期:1996-07-15 00:00:00
abstract::HIV infects macrophages and microglia in the central nervous system (CNS). Mechanisms that enhance HIV macrophage/microglial tropism are not well understood. Here, we identify an HIV Env variant in the V4 region of gp120, Asp 386 (D386), that eliminates an N-linked glycosylation site at position 386, enhances viral re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.05.029
更新日期:2007-10-10 00:00:00
abstract::Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral ge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.12.057
更新日期:2011-03-30 00:00:00
abstract::We present a case of sexual transmission of HIV-1 predicted to have CXCR4-tropism during male-to-male sexual exposure. Phylogenetic analyses exclude cell-free virus in the seminal plasma of the source partner and possibly point to the seminal cells as the origin of the transmission event. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.010
更新日期:2012-12-05 00:00:00
abstract::Stress granule (SG)-like antiviral granules (AVG) had been found in some vaccinia virus infection conditions and shown to repress translation. Similar RNA granules are also associated with translational inhibition and poxvirus restriction mediated by the host restriction factor SAMD9, but their function is less clear....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.01.007
更新日期:2019-03-01 00:00:00
abstract::Most adenoviral vectors use in gene therapy protocols derive from species C. However, expression of the primary receptor (human Coxsackievirus and Adenovirus receptor, hCAR) for these AdV is variable on cancer cells. In vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therap...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.023
更新日期:2004-04-10 00:00:00
abstract::Human nectin-1 (HveC, Prr1), a member of the immunoglobulin superfamily and a receptor for the entry of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1), binds to viral gD. For HSV-1, HSV-2, and PRV, the gD-binding region of nectin-1 has been localized to the N-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0989
更新日期:2001-07-05 00:00:00
abstract::The hepatitis B virus core open reading frame with and without the precore domain was expressed in insect cells using a baculovirus expression system. Precore antigen was not properly processed in insect cells and was present in highly insoluble cytoplasmic aggregates. Core antigen without the precore domain formed co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90247-o
更新日期:1990-05-01 00:00:00
abstract::Three distinct species of nucleocapsids of equine herpesvirus type-1, designated as either light (L), intermediate (I), or heavy (H) on the basis of their densities in Renografin-76 density gradients (Perdue et al. 1975), were characterized with respect to their amino acid content, DNA content, and role in the maturat...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::Feline foamy virus (FeFV) belongs to the group of spumaretroviruses that contain in addition to gag, pol, and env accessory genes collectively called bel genes. Primate FVs have been shown to utilize internal promoters in addition to the 5' LTR promoters. In contrast to other known retroviruses, the FV pol genes are e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9113
更新日期:1998-05-10 00:00:00
abstract::Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identifie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1147
更新日期:1993-03-01 00:00:00
abstract::We previously showed that reduced infectivity of HIV with incompletely processed capsid-spacer protein 1 (CA-SP1) is rescued by cellular activation or increased expression of HSP90AB1, a member of the cytosolic heat shock protein 90 family. Here we show that HSP90AB1 is present in HIV virions and that HSP90AB1, but no...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.005
更新日期:2013-02-05 00:00:00
abstract::Infection with herpes simplex virus leads to amplification of SV40 DNA in various SV40-transformed cells. In earlier studies with the SV40-transformed hamster cell line Elona two different types of DNA amplification could be identified: (i) Bidirectional overreplication of chromosomally integrated SV40 DNA expanding i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90586-2
更新日期:1989-12-01 00:00:00
abstract::RNA copies, synthesized in vitro, of the virulent satellite (RNA C) of turnip crinkle virus (TCV) infect plants when coinoculated with helper virus RNA. RNA C is a small linear RNA of about 355 bases which intensifies TCV symptoms in infected plants. Full-length cDNA copies of the satellite were inserted in an express...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90445-4
更新日期:1987-01-01 00:00:00
abstract::The genome of the sigma rhabdovirus of Drosophila melanogaster consists of six genes in the order 3' N-2-3-4-G-L 5'. The nucleotide sequences of the N and of the fourth genes were determined from cDNA clones. Each gene contained a single long open reading frame encoding polypeptides with predicted MW of 50 and 25 kDa,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1177
更新日期:1994-04-01 00:00:00
abstract::Rabies continues to poses serious threats to the public health in many countries. The development of novel inexpensive, safe and effective vaccines has become a high priority for rabies control worldwide. We previously generated a novel recombinant rabies vaccine by cloning rabies virus glycoprotein into a chimpanzee ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.07.022
更新日期:2019-10-01 00:00:00
abstract::The single-stranded DNA-binding protein LEF-3 of Autographa californica multinucleocapsid nucleopolyhedrovirus consists of 385 amino acid residues, forms oligomers, and promotes Mg2+-independent unwinding of DNA duplexes and annealing of complementary DNA strands. Partial proteolysis revealed that the DNA-binding doma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.027
更新日期:2006-03-15 00:00:00
abstract::The DNAs of the Cas-Br-E MuLV-induced leukemias always contain somatically acquired mink cell focus-forming (MCF) recombinant proviruses. MCF recombinants could be involved during leukemogenesis at both preleukemic times and in late-stage tumors. Among the Cas-Br-E-induced non-T-, non-B-cell leukemias, viral integrati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90241-g
更新日期:1992-12-01 00:00:00
abstract::Lymphoid cell lines were derived from three avian leukosis virus (ALV)-induced lymphomas. These cell lines contained proviral DNA sequences integrated upstream from the c-myc proto-oncogene, expressed increased levels of c-myc RNA, and were tumorigenic in syngeneic animals. While cell surface immunoglobulin (IgM) was ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90312-5
更新日期:1985-07-15 00:00:00
abstract::Hepatitis B virus synthesizes multiple spliced RNAs that can be reverse transcribed into viral DNA. We thoroughly characterized the contribution of spliced RNAs to DNA synthesis in transfected cultures of Huh7 and HepG2 cells. We found that up to 50% of DNA within intracellular capsids is derived from five spliced RNA...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.021
更新日期:2008-09-15 00:00:00
abstract::It is now well recognized that mutations in the hepatitis B virus (HBV) genome occur during the natural course of chronic viral infection. Regions of the viral genome that are frequently affected by such mutations, rearrangements, and/or deletions generally involve the precore promoter, precore, and core as well as th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8594
更新日期:1997-07-07 00:00:00
abstract::N-tropic murine leukemia viruses have been observed in connection with radiation-induced osteosarcomagenesis in BALB/c mice. We have investigated the bone disease-inducing potential of molecularly cloned, BALB/c-derived N-tropic viruses in the random-bred NMRI mouse strain. The germ-line virus and an exogenous virus i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90171-m
更新日期:1990-12-01 00:00:00
abstract::How the H7N9 avian influenza virus gained the distinct ability to infect humans is unclear. Pigs are an important host in influenza virus ecology because they are susceptible to infection with both avian and human influenza viruses and are often involved in interspecies transmission. Here, we passaged one avian isolat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.01.016
更新日期:2014-03-01 00:00:00
abstract::In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian clade 2.3.4.4 H5 highly pathogenic avian influenza (HPAI) virus, initially affecting mainly wild birds and few backyard and commercial poultry premises. To better model the outbreak, the pathogenesis and transmission dynamics of representative Eu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.019
更新日期:2016-07-01 00:00:00
abstract::The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) rais...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1427
更新日期:1994-08-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is a major cause of bronchiolitis and pneumonia in infants and pneumonia virus of mice (PVM) causes similar disease. BALB/c mice are highly susceptible, while C57BL/6 mice are more resistant to PVM. IL-12 was significantly more up-regulated in response to PVM infection in BALB/c than ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.020
更新日期:2016-10-01 00:00:00
abstract::We have previously adapted an experimental system which supports autonomous replication of human papillomavirus-11 (HPV-11) genomic DNA in a human squamous carcinoma cell line (SCC-4) following electroporation of linearized viral DNA. This system allows evaluation of HPV-11 transcription in the context of replicating ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0298
更新日期:1996-06-01 00:00:00