当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Loss of the N-linked glycosylation site at position 386 in the HIV envelope V4 region enhances macrophage tropism and is associated with dementia.

Loss of the N-linked glycosylation site at position 386 in the HIV envelope V4 region enhances macrophage tropism and is associated with dementia.

Abstract：

:HIV infects macrophages and microglia in the central nervous system (CNS). Mechanisms that enhance HIV macrophage/microglial tropism are not well understood. Here, we identify an HIV Env variant in the V4 region of gp120, Asp 386 (D386), that eliminates an N-linked glycosylation site at position 386, enhances viral replication in macrophages, and is present at a higher frequency in AIDS patients with HIV-associated dementia (HAD) compared with non-HAD patients. D386 enhances HIV entry and replication in macrophages but not in microglia or peripheral blood mononuclear cells, possibly due to differential glycosylation in these cell types. A D386N mutation in the UK1br Env, which restores the N-linked glycan site, reduced neutralization sensitivity to the IgG1b12 (b12) monoclonal antibody, which recognizes a conserved neutralization epitope that overlaps the CD4 binding site. Molecular modeling suggested that loss of the glycan at position 386 increases exposure of the CD4 and b12 binding sites on gp120. Loss of a glycan at 386 was more frequent in Envs from HAD patients (26%; n=185) compared with non-HAD patients (7%; n=99; p<0.001). The most significant association of these Env variants with HAD was in blood or lymphoid tissue rather than brain. These findings suggest that increased exposure of the b12 epitope overlapping the CD4 binding site via elimination of a glycan at position 386 is associated with enhanced HIV macrophage tropism, and provide evidence that determinants of macrophage and microglia tropism are overlapping but distinct.

journal_name

Virology

journal_title

Virology

authors

Dunfee RL,Thomas ER,Wang J,Kunstman K,Wolinsky SM,Gabuzda D

doi

10.1016/j.virol.2007.05.029

subject

Has Abstract

pub_date

2007-10-10 00:00:00

pages

222-34

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(07)00385-6

journal_volume

367

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Differential expression of the HHV-8 vGCR cellular homolog gene in AIDS-associated and classic Kaposi's sarcoma: potential role of HIV-1 Tat.

    abstract：:Human herpesvirus 8 (HHV-8) has been causally linked to Kaposi's sarcoma (KS). There is significant homology between some HHV-8 genes and cellular genes including D-type cyclin (vCYC), G protein coupled receptor (vGCR), macrophage inflammatory proteins (vMIP-I, vMIP-II), bcl-2 (vBCL2), interferon regulatory factor-1 (...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0125

    authors： Yen-Moore A,Hudnall SD,Rady PL,Wagner RF Jr,Moore TO,Memar O,Hughes TK,Tyring SK

    更新日期：2000-02-15 00:00:00

  • Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus.

    abstract：:Prostaglandin E₂ (PGE₂) is an arachidonic acid (AA)-derived signaling molecule that can influence host immune responses to infection or vaccination. In this study, we investigated PGE₂ production in vitro by cells infected with the poxvirus vaccine strain, modified vaccinia Ankara virus (MVA). Human THP-1 cells, murin...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.03.019

    authors： Pollara JJ,Spesock AH,Pickup DJ,Laster SM,Petty IT

    更新日期：2012-07-05 00:00:00

  • Drosophila miR-956 suppression modulates Ectoderm-expressed 4 and inhibits viral replication.

    abstract：:Small non-coding microRNAs (miRNAs) can modulate the outcome of virus infection. Here we explore the role of miRNAs in insect-virus interactions, in vivo, using the natural Drosophila melanogaster-Drosophila C virus (DCV) model system. Comparison of the miRNA expression profiles in DCV-infected and uninfected flies sh...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.12.009

    authors： Monsanto-Hearne V,Tham ALY,Wong ZS,Asgari S,Johnson KN

    更新日期：2017-02-01 00:00:00

  • A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus.

    abstract：:The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.04.046

    authors： Truong HM,Lu Z,Kutish GF,Galeota J,Osorio FA,Pattnaik AK

    更新日期：2004-08-01 00:00:00

  • β-HPV 8E6 combined with TERT expression promotes long-term proliferation and genome instability after cytokinesis failure.

    abstract：:Human papillomavirus (HPV) is a family of viruses divided into five genera: alpha, beta, gamma, mu, and nu. There is an ongoing discussion about whether beta genus HPVs (β-HPVs) contribute to cutaneous squamous cell carcinoma (cSCC). The data presented here add to this conversation by determining how a β-HPV E6 protei...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.07.016

    authors： Dacus D,Riforgiate E,Wallace NA

    更新日期：2020-10-01 00:00:00

  • De novo reverse transcription of HTLV-1 following cell-to-cell transmission of infection.

    abstract：:Analogous to transmission of human T-cell leukemia virus type 1 (HTLV-1) in vivo, an in vitro cell-to-cell infection model was established by coculturing MT-2 cells as virus donors and HUT78 cells as recipients. At a donor:recipient ratio of 1:2, cell fusion occurred and a new round of HTLV-1 genome replication was in...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9111

    authors： Benovic S,Kok T,Stephenson A,McInnes J,Burrell C,Li P

    更新日期：1998-05-10 00:00:00

  • Reduction of soluble dipeptidyl peptidase 4 levels in plasma of patients infected with Middle East respiratory syndrome coronavirus.

    abstract：:Dipeptidyl peptidase 4 (DPP4) is a receptor for MERS-CoV. The soluble form of DPP4 (sDPP4) circulates systematically and can competitively inhibit MERS-CoV entry into host cells. Here, we measured the concentration of sDPP4 in the plasma and sputa of 14 MERS-CoV-infected patients of various degrees of disease severity...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.03.015

    authors： Inn KS,Kim Y,Aigerim A,Park U,Hwang ES,Choi MS,Kim YS,Cho NH

    更新日期：2018-05-01 00:00:00

  • The cowpox virus serpin SPI-3 complexes with and inhibits urokinase-type and tissue-type plasminogen activators and plasmin.

    abstract：:The orthopoxvirus serpin SPI-3 is N-glycosylated and suppresses fusion between infected cells. Although SPI-3 contains motifs conserved in inhibitory serpins, no proteinase inhibition by SPI-3 has been demonstrated, and mutations within the serpin reactive center loop (RCL) do not affect the ability to regulate cell f...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0377

    authors： Turner PC,Baquero MT,Yuan S,Thoennes SR,Moyer RW

    更新日期：2000-07-05 00:00:00

  • Packaging of DNA in cauliflower mosaic virus and bacteriophage SD studied with magnetic birefringence.

    abstract：:Magnetic birefringence measurements are reported on solutions of two double-stranded DNA containing viruses: icosahedral cauliflower mosaic virus (CaMV) and SD, an isometric bacteriophage with a short tail. Assuming the signal comes predominantly from the DNA, constraints can be put on its packing geometry. The anisot...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90232-1

    authors： Torbet J,Timmins PA,Lvov Y

    更新日期：1986-12-01 00:00:00

  • Persistent coinfection of T lymphocytes with HTLV-II and HIV and the role of syncytium formation in HIV-induced cytopathic effect.

    abstract：:We previously demonstrated a high permissiveness of HTLV-II-transformed T lymphocytes (C3) to human immunodeficiency virus (HIV) infection in vitro, and that this infection results in the lysis of cells (D.C. Montefiori and W.M. Mitchell (1986) Virology 155, 726-731). We now show that a small percentage of HIV-infecte...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90008-0

    authors： Montefiori DC,Mitchell WM

    更新日期：1987-10-01 00:00:00

  • Recruitment of activated IRF-3 and CBP/p300 to herpes simplex virus ICP0 nuclear foci: Potential role in blocking IFN-beta induction.

    abstract：:The host innate response to viral infection includes the production of interferons, which is dependent on the coordinated activity of multiple transcription factors. Herpes simplex virus 1 (HSV-1) has been shown to block efficient interferon expression by multiple mechanisms. We and others have demonstrated that HSV-1...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.10.028

    authors： Melroe GT,Silva L,Schaffer PA,Knipe DM

    更新日期：2007-04-10 00:00:00

  • The structure of adeno-associated virus serotype 3B (AAV-3B): insights into receptor binding and immune evasion.

    abstract：:Adeno-associated viruses (AAVs) are leading candidate vectors for human gene therapy. AAV serotypes have broad cellular tropism and use a variety of cellular receptors. AAV serotype 3 binds to heparan sulfate proteoglycan prior to cell entry and is serologically distinct from other serotypes. The capsid features that ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.03.027

    authors： Lerch TF,Xie Q,Chapman MS

    更新日期：2010-07-20 00:00:00

  • Identification of constrained peptides that bind to and preferentially inhibit the activity of the hepatitis C viral RNA-dependent RNA polymerase.

    abstract：:A class of disulfide constrained peptides containing a core motif FPWG was identified from a screen of phage displayed library using the HCV RNA-dependent RNA polymerase (NS5B) as a bait. Surface plasmon resonance studies showed that three highly purified synthetic constrained peptides bound to immobilized NS5B with e...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00313-1

    authors： Amin A,Zaccardi J,Mullen S,Olland S,Orlowski M,Feld B,Labonte P,Mak P

    更新日期：2003-08-15 00:00:00

  • RNA helicase MOV10 functions as a co-factor of HIV-1 Rev to facilitate Rev/RRE-dependent nuclear export of viral mRNAs.

    abstract：:Human immunodeficiency virus type 1 (HIV-1) exploits multiple host factors during its replication. The REV/RRE-dependent nuclear export of unspliced/partially spliced viral transcripts needs the assistance of host proteins. Recent studies have shown that MOV10 overexpression inhibited HIV-1 replication at various step...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.08.026

    authors： Huang F,Zhang J,Zhang Y,Geng G,Liang J,Li Y,Chen J,Liu C,Zhang H

    更新日期：2015-12-01 00:00:00

  • HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin.

    abstract：:HIV-1 Vpu antagonizes the block to particle release mediated by CD317 (BST-2/HM1.24/Tetherin) via incompletely understood mechanisms. Vpu and CD317 partially reside in cholesterol-rich lipid rafts where HIV-1 budding preferentially occurs. Here we find that lipid raft association of ectopically expressed or endogenous...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.12.008

    authors： Fritz JV,Tibroni N,Keppler OT,Fackler OT

    更新日期：2012-03-01 00:00:00

  • Nucleotide and deduced amino acid sequence of the influenza neuraminidase genes of two equine serotypes.

    abstract：:Equine influenza is caused by two serotypes of type A influenza virus, EIV-A1 and EIV-A2. The complete nucleotide sequence of the neuraminidase (NA) genes of both the A1 (N7 subtype) and A2 (N8 subtype) serotype has been determined following cloning of full-length viral NA cDNAs into pBR322. Analysis of the deduced am...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90207-2

    authors： Dale B,Brown R,Miller J,White RT,Air GM,Cordell B

    更新日期：1986-12-01 00:00:00

  • Palmitoylation of the cysteine-rich endodomain of the SARS-coronavirus spike glycoprotein is important for spike-mediated cell fusion.

    abstract：:The SARS-coronavirus (SARS-CoV) is the etiological agent of the severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. The cytoplasmic portion of the S glycoprotein contains four cysteine-rich amino acid cl...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.10.034

    authors： Petit CM,Chouljenko VN,Iyer A,Colgrove R,Farzan M,Knipe DM,Kousoulas KG

    更新日期：2007-04-10 00:00:00

  • Transcriptional activity of human papillomavirus type 31b enhancer is regulated through synergistic interaction of AP1 with two novel cellular factors.

    abstract：:Transcription of human papillomaviruses (HPV) is regulated by enhancer sequences located in the upstream regulatory region. The factors regulating expression of one of the high risk genital HPV types, HPV 31b, were investigated using transient expression and protein binding assays. A region of 262 base pairs in length...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1390

    authors： Kyo S,Tam A,Laimins LA

    更新日期：1995-08-01 00:00:00

  • Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission.

    abstract：:A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we dem...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.09.028

    authors： Wu L,Martin TD,Carrington M,KewalRamani VN

    更新日期：2004-01-05 00:00:00

  • An insertion mutation in the pol gene of Moloney murine leukemia virus results in temperature-sensitive pol maturation and viral replication.

    abstract：:An insertion mutation in the pol gene of Moloney murine leukemia virus (M-MuLV) was found to render the virus temperature-sensitive for replication. A provirus containing a 12-bp insertion at the boundary between the reverse transcriptase (RT) and integrase (IN) domains induced the formation of mutant virions containi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90428-5

    authors： Tanese N,Roth M,Epstein H,Goff SP

    更新日期：1989-06-01 00:00:00

  • Small amino acid changes in the V3 loop of human immunodeficiency virus type 2 determines the coreceptor usage for CXCR4 and CCR5.

    abstract：:HIV-2 GH-1 is a molecular clone derived from an AIDS patient from Ghana. In contrast to the prototypic molecular clone ROD, GH-1 exhibits a narrow range of target cell specificity. By an infectious assay using HeLa-CD4 cells stably transfected with an HIV-1 LTR-beta-galactosidase reporter gene and transiently expressi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0006

    authors： Isaka Y,Sato A,Miki S,Kawauchi S,Sakaida H,Hori T,Uchiyama T,Adachi A,Hayami M,Fujiwara T,Yoshie O

    更新日期：1999-11-10 00:00:00

  • A system for the analysis of BKV non-coding control regions: application to clinical isolates from an HIV/AIDS patient.

    abstract：:The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BKV exists as archetype virus and rearranged variants, w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.08.032

    authors： Broekema NM,Abend JR,Bennett SM,Butel JS,Vanchiere JA,Imperiale MJ

    更新日期：2010-11-25 00:00:00

  • Vaccinia virus-mediated inhibition of host protein synthesis involves neither degradation nor underphosphorylation of components of the cap-binding eukaryotic translation initiation factor complex eIF-4F.

    abstract：:Recent reports indicated that vaccinia virus late mRNAs contain a unique 5' poly(A) leader sequence and that the in vitro translation of these mRNAs may be relatively cap-independent. These observations led us to examine the possibility that the mechanism of inhibition of host protein synthesis by vaccinia virus, like...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90556-5

    authors： Schnierle BS,Moss B

    更新日期：1992-06-01 00:00:00

  • Replication of hepatitis B virus in a hepatocellular carcinoma.

    abstract：:Hepatitis B virus transcripts and DNA from paired samples of neoplastic and nonneoplastic liver tissue of HBsAg seropositive patients were analyzed. The data obtained support the view that transcription of integrated DNA is frequent, both in neoplastic as well as in nonneoplastic liver tissue. In the case of one patie...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90064-x

    authors： Loncarević IF,Schranz P,Zentgraf H,Liang XH,Herrmann G,Tang ZY,Schröder CH

    更新日期：1990-01-01 00:00:00

  • Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001: comparison with the South African strain SAAR-1776 and other flaviviruses.

    abstract：:Here we describe the complete genome sequences of two strains of Usutu virus (USUV), a mosquito-borne member of the genus Flavivirus in the Japanese encephalitis virus (JEV) serogroup. USUV was detected in Austria in 2001 causing a high mortality rate in blackbirds; the reference strain (SAAR-1776) was isolated in 195...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.08.005

    authors： Bakonyi T,Gould EA,Kolodziejek J,Weissenböck H,Nowotny N

    更新日期：2004-10-25 00:00:00

  • Transcriptive complex of Newcastle disease virus. II. Structural and functional assembly associated with the cytoskeletal framework.

    abstract：:The transcriptive complex, the nucleocapsid with the viral RNA-synthesizing activity, of Newcastle disease virus (NDV) contains three protein components, the major structural subunit (NP) and two associated proteins (P and L) involved in the RNA synthesis. We studied the pathway of these proteins from synthesis to ass...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90132-1

    authors： Hamaguchi M,Nishikawa K,Toyoda T,Yoshida T,Hanaichi T,Nagai Y

    更新日期：1985-12-01 00:00:00

  • High-affinity Rep-binding is not required for the replication of a geminivirus DNA and its satellite.

    abstract：:The 682-nt satellite DNA (sat-DNA) of Tomato leaf curl virus (TLCV) depends on the helper virus for its replication. In contrast to the strict specificity that exists in each geminivirus for its cognate replication associated protein (Rep), TLCV sat-DNA can utilize Rep encoded by distinct geminiviruses. We have used a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1671

    authors： Lin B,Akbar Behjatnia SA,Dry IB,Randles JW,Rezaian MA

    更新日期：2003-01-20 00:00:00

  • Analysis of the virulence modulating region of potato spindle tuber viroid (PSTVd) by site-directed mutagenesis.

    abstract：:A series of nucleotide substitutions (G46----C; C47----A; C315----U; U317----C) were introduced into the virulence modulating region of the intermediate strain of potato spindle tuber viroid (PSTVd) in order to examine their effect upon viroid infectivity and pathogenicity with the presence of all four mutations resul...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90468-5

    authors： Hammond RW

    更新日期：1992-04-01 00:00:00

  • Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus.

    abstract：:The lentivirus caprine arthritis-encephalitis virus (CAEV) is closely related by nucleotide sequence homology to visna virus and other sheep lentiviruses and shows less similarity to the other animal and human lentiviruses. The genomic organization of CAEV is very similar to that of visna virus and the South African o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90303-9

    authors： Saltarelli M,Querat G,Konings DA,Vigne R,Clements JE

    更新日期：1990-11-01 00:00:00

  • A cytopathic and a cell culture adapted hepatitis A virus strain differ in cell killing but not in intracellular membrane rearrangements.

    abstract：:Aside from a common gene organization shared with other picornaviruses, hepatitis A virus (HAV) is characterized by its slow-growth phenotype, the inability to shut off host macromolecular synthesis, and, in general, lack of cytopathic (cp) effects in permissive cell cultures. Nevertheless, several cp HAV strains have...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0070

    authors： Gosert R,Egger D,Bienz K

    更新日期：2000-01-05 00:00:00