Abstract:
:Human herpesvirus 8 (HHV-8) has been causally linked to Kaposi's sarcoma (KS). There is significant homology between some HHV-8 genes and cellular genes including D-type cyclin (vCYC), G protein coupled receptor (vGCR), macrophage inflammatory proteins (vMIP-I, vMIP-II), bcl-2 (vBCL2), interferon regulatory factor-1 (vIRF1), interleukin-6 (vIL6), and complement-binding protein (vCBP). In this study, we analyzed expression of these viral homologs and HIV-1 Tat by reverse-transcriptase polymerase chain reaction (RT-PCR) coupled with Southern blot hybridization in AIDS-KS (AKS) tissue, classic KS tissue(CKS), and peripheral blood mononuclear cells, and phorbol ester (TPA)-treated and untreated HHV-8 positive lymphoma cells (BCBL1). While vCYC (AKS 6 of 6; CKS 3 of 3), vMIP-I (AKS 5 of 6, CKS 3 of 3), vBCL2 (AKS 6 of 6; CKS 3 of 3), and vIRF1 (AKS 5 of 6, CKS 3 of 3) transcripts were detected in both AKS and CKS, vGCR and HIV-1 Tat were expressed only in AKS samples (vGCR: AKS 3 of 6, CKS 0 of 3; Tat: AKS 4 of 6, CKS 0 of 3). vMIPII, vCBP, and vIL6 expression were not detected in any KS samples. Since vGCR expression is limited to AKS, it is possible that vGCR is activated by HIV-1 Tat. These results suggest that HIV-1 Tat may contribute to AKS pathogenesis through the tumorigenic and angiogenic effects of vGCR.
journal_name
Virologyjournal_title
Virologyauthors
Yen-Moore A,Hudnall SD,Rady PL,Wagner RF Jr,Moore TO,Memar O,Hughes TK,Tyring SKdoi
10.1006/viro.1999.0125subject
Has Abstractpub_date
2000-02-15 00:00:00pages
247-51issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(99)90125-3journal_volume
267pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Mouse hepatitis virus strain A59 (MHV-A59) encodes within the 22-kb gene 1 a large polyprotein containing three proteinase domains with proven or predicted cysteine catalytic residues. E64d, a specific, irreversible inhibitor of cysteine (thiol) proteinases, inhibits the processing of the gene 1 polyprotein. Specifica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1123
更新日期:1995-04-01 00:00:00
abstract::Tobacco mosaic virus (TMV) is widespread in China and causes considerable economic losses to tobacco production. The molecular epidemiology of this virus is, however, poorly understood. In this study, we sequenced the genomes of 51 TMV isolates from five tobacco-producing regions in China and investigated the dispersa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.12.001
更新日期:2019-02-01 00:00:00
abstract::Many tumors, including cervical carcinoma, show dysregulated expression of the microRNA processing machinery, specifically DROSHA and DICER. Some cervical cancers exhibit chromosome 5p amplifications and DROSHA is the most significantly upregulated transcript and is observed in all tumors with 5p gain. DROSHA and DICE...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.04.022
更新日期:2017-07-01 00:00:00
abstract::Of five globally important VP7 (G) serotypes (G1-4 and 9) of group A rotaviruses (the single most important etiologic agents of infantile diarrhea worldwide), G9 continues to attract considerable attention because of its unique natural history. Serotype G9 rotavirus was isolated from a child with diarrhea first in the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.11.006
更新日期:2005-02-05 00:00:00
abstract::Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.01.027
更新日期:2007-07-05 00:00:00
abstract::Inflammasome activation is gaining recognition as an important mechanism for protection during viral infection. Here, we investigate whether Rift Valley fever virus, a negative-strand RNA virus, can induce inflammasome responses and IL-1β processing in immune cells. We have determined that RVFV induces NLRP3 inflammas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.11.015
更新日期:2014-01-20 00:00:00
abstract::3H-labelled complementary DNA (cDNA) was reverse transcribed from the RNA of three biologically distinct isolates of bean yellow mosaic virus (BYMV-G, Q, and S) by the random primer method of Taylor et al. [Biochim. Biophys. Acta 442, 324-330 (1976)]. Excess virus RNA was hybridized with each of the cDNAs and percenta...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90074-x
更新日期:1981-04-30 00:00:00
abstract::It has been suggested that, in addition to viral proteins, host proteins are involved in RNA virus replication. In this study the RNA helicase domain of the Tobacco mosaic virus (TMV) replicase proteins was used as bait in the yeast two-hybrid system to identify tobacco proteins with a putative role in TMV replication...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1332
更新日期:2002-04-10 00:00:00
abstract::HIV-1 Rev escorts unspliced viral mRNAs out of the nucleus of infected cells, which allows formation of infectious HIV-1 virions. We have identified a putative DEAD box (Asp-Glu-Ala-Asp) RNA helicase, DDX1, as a cellular co-factor of Rev, through yeast and mammalian two-hybrid systems using the N-terminal motif of Rev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.09.039
更新日期:2004-12-20 00:00:00
abstract::A full-length cDNA clone of the U1 (common) strain of tobacco mosaic virus (TMV) was constructed, and highly infectious transcripts were produced in vitro using bacteriophage T7 RNA polymerase. Frameshift mutations designed to cause premature termination of translation were introduced into either the 30-kDa movement p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90475-q
更新日期:1991-03-01 00:00:00
abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0002
更新日期:1996-01-01 00:00:00
abstract::Adenovirus vectors are known to induce certain genes and impact innate response networks, but a broad understanding of this process and its mechanisms is currently lacking. For this reason, we chose to investigate and characterize Ad innate immunity using homogeneous, primary MEF cells replete with all the elements of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.041
更新日期:2007-02-20 00:00:00
abstract::The P1 plasmid partition system depends on ParA-ParB proteins acting on centromere-like parS sites for a faithful plasmid segregation during the Escherichia coli cell cycle. In vivo we placed parS into host E. coli chromosome and on a Sop(+) F plasmid and found that the stability of a P1 plasmid deleted for parA-parB ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.09.027
更新日期:2011-12-20 00:00:00
abstract::Recently, we reported that in vitro and in vivo persistent infection of neurons by lymphocytic choriomeningitis virus (LCMV) downregulated GAP43 expression, a protein involved in neuronal plasticity associated with learning and memory. Here, we investigated the transcriptional and posttranscriptional events involved. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8458
更新日期:1997-04-14 00:00:00
abstract::Antisera with Fiji disease virus (FDV) protein-specific and double-stranded (ds) RNA-specific antibodies were prepared by injecting rabbits with highly purified preparations of FDV cores. Electron microscopic examination of negatively stained core preparations disclosed the presence of icosahedral particles, about 54 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(79)90495-1
更新日期:1979-06-01 00:00:00
abstract::The recent development of arterivirus full-length cDNA clones makes possible the construction of chimeric arteriviruses for fundamental and applied studies. Using an equine arteritis virus (EAV) infectious cDNA clone, we have engineered chimeras in which the ectodomains of the two major envelope proteins, the glycopro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1074
更新日期:2001-09-30 00:00:00
abstract::We compared the outcome of different cellular and viral factors on the regulation of the HPV-16 early viral gene expression in trophoblastic and cervical cancer cells. A high variability of the long control (LCR) activity was observed, prompting us to evaluate the role of secreted factors in the control of the early g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.12.056
更新日期:2011-03-30 00:00:00
abstract::Previous research has indicated that the adenovirus protein complex named RID, derived from the E3 transcription unit, functions to remove the receptors named Fas/Apo1/CD95 (Fas) and epidermal growth factor receptor (EGFR) from the surface of cells. (The RID complex is composed of the RIDalpha and RIDbeta polypeptides...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1580
更新日期:2002-09-15 00:00:00
abstract::Mixed infection of nonpermissive bacteria by amber mutant and wild-type T4 phage reduces the burst size in a manner that depends on the nature of the gene product eliminated by the mutation. While the genotypic proportions produced by such mixedly infected cells were directly proportional to those in the starting mixt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90011-4
更新日期:1984-08-01 00:00:00
abstract::Many viruses utilize molecular chaperone heat shock protein 90 (Hsp90) for protein folding and stabilization, however, the role of Hsp90 in herpesvirus lifecycle is obscure. Here, we provide evidence that Hsp90 participates in pseudorabies virus (PRV) replication. Viral growth kinetics assays show that Hsp90 inhibitor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.10.013
更新日期:2021-01-15 00:00:00
abstract::Previously, we studied the envelope (E) gene of dengue virus and reported that dengue-3 virus is present as a quasispecies. To investigate the extent of intrahost sequence variation of other dengue viral genes, we examined in this study the capsid (C) gene and the nonstructural gene, NS2B, derived directly from plasma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1635
更新日期:2002-11-10 00:00:00
abstract::Repression of human papillomavirus (HPV) E6 and E7 oncogenes in established cervical carcinoma cell lines causes senescence due to reactivation of cellular tumor suppressor pathways. Here, we determined whether ongoing expression of HPV16 or HPV18 oncogenes is required for the proliferation of primary human cervical c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.10.012
更新日期:2012-01-05 00:00:00
abstract::Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90287-3
更新日期:1984-04-15 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) glycoprotein B (gB) is a multifunctional glycoprotein required for infectivity; it is thought to promote fusion of the viral envelope with the cell membrane and entry of virions into cells. To map the antigenic and functional domains on gB, we constructed amino terminal derivatives lacki...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90102-5
更新日期:1989-09-01 00:00:00
abstract::Segment 7 (M) of the cold-adapted live influenza A virus vaccine plays a primary role in the ability of this virus to interfere with the replication of wild-type influenza A viruses. This conclusion is based on several lines of evidence. Single gene reassortant viruses derived by crossing influenza A/Ann Arbor/6/60 (H...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90011-y
更新日期:1991-01-01 00:00:00
abstract::The influenza RNA polymerase is known to catalyse three distinct copying activities: (i) transcription of minus-sense virion RNA (vRNA) into mRNA, (ii) transcription of vRNA into full-length complementary RNA (cRNA), and (iii) transcription of cRNA to vRNA. Ever since the discovery of the conserved 13 and 12 long sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90079-5
更新日期:1992-01-01 00:00:00
abstract::The synthesis of linear duplex replicative structures (monomers, head-to-head, and tail-to-tail dimers) is an important hallmark of the productive phase of the adeno-associated virus (AAV) life cycle. These structures are generated by a strand-displacement replication mechanism and believed to be a reservoir for singl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0504
更新日期:2000-09-30 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68) is used as a model to study gammaherpesvirus pathogenesis both in tissue culture systems and in vivo. We used a gene-trapping approach to get insight into cellular factors involved in MHV68 infection. By generating a library of gene-trapped CHO cells, we were able to isolate several ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.12.004
更新日期:2008-04-10 00:00:00
abstract::Mouse mammary tumour virus (MMTV) encodes a viral superantigen (Sag) and a negative acting factor (Naf) which share parts of their coding sequence. Using 2-dimensional gel electrophoresis (2D-DIGE), we could show that at least 10 different cellular proteins were differentially expressed in Naf positive cells. Also, lu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.029
更新日期:2006-03-01 00:00:00
abstract::The noncapsid protein expressed from ORF-206 of turnip yellow mosaic virus (TYMV) is autocatalytically processed by a papain-like protease, producing N-terminal 150-kDa and C-terminal 70-kDa proteins. By introducing two methionine residues near the N-terminus of the 70-kDa protein, we have obtained N-terminal amino ac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0131
更新日期:1996-03-01 00:00:00