Coronavirus protein processing and RNA synthesis is inhibited by the cysteine proteinase inhibitor E64d.

abstract：:We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-...

journal_title：Virology

authors： Green S,Kurane I,Pincus S,Paoletti E,Ennis FA

更新日期：1997-08-04 00:00:00

abstract：:Alternative splicing of pre-mRNAs plays a critical role in maximizing the coding capacity of the small parvovirus genome. The small-intron region of minute virus of mice (MVM) pre-mRNAs undergoes an unusual pattern of overlapping alternative splicing, using two donors, D1 and D2, and two acceptors, A1 and A2, within a...

journal_title：Virology

authors： Haut DD,Pintel DJ

更新日期：1999-05-25 00:00:00

abstract：:Human infection with H7N9 virus has provoked global public health concern due to the substantial morbidity and mortality. Neuraminidase inhibitors (NAIs) are used as first-line drugs to treat the infection. However, virus quasispecies can evolve rapidly under drug pressure, which may alter various biological character...

journal_title：Virology

authors： Ge Y,Chi Y,Min X,Zhao K,Wu B,Wu T,Zhu X,Shi Z,Zhu F,Cui L

更新日期：2019-10-01 00:00:00

abstract：:G1/S transition of the cell cycle is blocked when cells are cultured without cell adhesion, even if nutrients and serum are present. Shift experiments involving transfer from adhesion to suspension cultures have revealed that cell adhesion in the early half of G1 phase is required for induction of DNA synthesis. We fo...

journal_title：Virology

authors： Inoue H,Yamashita A,Hakura A

更新日期：1996-11-01 00:00:00

abstract：:A recombination event at the 3' end of RNA 3 in a pseudorecombinant virus (C1C2T3) having RNAs 1 and 2 from TrK7-cucumber mosaic virus (CMV) and RNA 3 from P-tomato aspermy virus (TAV) was detected by ribonuclease protection assay (RPA). Sequence analysis of the 3' end of RNA 3 of C1C2T3 and of its parental CMV and TA...

journal_title：Virology

authors： Fernández-Cuartero B,Burgyán J,Aranda MA,Salánki K,Moriones E,García-Arenal F

更新日期：1994-09-01 00:00:00

abstract：:We have studied the population dynamics in response to selective drug pressure of mixtures of wild-type and mutant HIV viruses exposed to either an inhibitor of the viral protease or a nonnucleoside allosteric inhibitor of the viral reverse transcriptase. In order to quantitate mutant virus present in a mixed populati...

journal_title：Virology

authors： Rayner MM,Cordova B,Jackson DA

更新日期：1997-09-15 00:00:00

abstract：:Bipartite geminiviruses are small, plant-infecting viruses with genomes composed of circular, single-stranded DNA molecules, designated A and B. Although they are closely related genetically, individual bipartite geminiviruses frequently exhibit host-specific adaptation. Two such viruses are bean golden mosaic virus (...

journal_title：Virology

authors： Petty IT,Carter SC,Morra MR,Jeffrey JL,Olivey HE

更新日期：2000-11-25 00:00:00

abstract：:Vitis vinifera cultivars Zinfandel-1A and Mission were found to harbor different grapevine yellow speckle viroid-1 (GYSVd-1) variants and characterized to define the relationship to yellow speckle (YS) and vein-banding (VB) diseases. Products from the left terminal (T1), pathogenic (P), and a portion of the central (C...

journal_title：Virology

authors： Szychowski JA,Credi R,Reanwarakorn K,Semancik JS

更新日期：1998-09-01 00:00:00

abstract：:We have used a microinjection approach to identify a domain of the simian virus 40 (SV40) structural proteins Vp2 and Vp3(Vp2/3) responsible for their nuclear transport. By using both synthetic peptides, containing small regions of Vp2/3 conjugated to bovine serum albumin (BSA), and beta-galactosidase-Vp3 fusion prote...

journal_title：Virology

authors： Clever J,Kasamatsu H

更新日期：1991-03-01 00:00:00

abstract：:Nipah virus (NiV) causes severe encephalitis in humans, with high mortality. NiV nonstructural C protein (NiV-C) is essential for its pathogenicity, but its functions are unclear. In this study, we focused on NiV-C trafficking in cells and found that it localizes predominantly in the cytoplasm but partly in the nucleu...

journal_title：Virology

authors： Horie R,Yoneda M,Uchida S,Sato H,Kai C

更新日期：2016-10-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in cont...

journal_title：Virology

authors： Mahmoud NF,Jasirwan C,Kanemoto S,Wakata A,Wang B,Hata Y,Nagamata S,Kawabata A,Tang H,Mori Y

更新日期：2016-03-01 00:00:00

abstract：:CD8 T cells play an important role in controlling Ebola infection and in mediating vaccine-induced protective immunity, yet little is known about antigenic targets in Ebola that are recognized by CD8 T cells. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mic...

journal_title：Virology

authors： Simmons G,Lee A,Rennekamp AJ,Fan X,Bates P,Shen H

更新日期：2004-01-05 00:00:00

abstract：:Epstein-Barr virus (EBV) is known to infect B cells and epithelial cells. We and others have shown that EBV can also infect a subset of thymocytes. Infection of thymocytes was accompanied by the appearance of linear EBV genome within 8 hr of infection. Circularization of the EBV genome was not detected. This is in con...

journal_title：Virology

authors： Kelleher CA,Paterson RK,Dreyfus DH,Streib JE,Xu JW,Takase K,Jones JF,Gelfand EW

更新日期：1995-04-20 00:00:00

abstract：:We demonstrate that the AL2 gene of Cabbage leaf curl virus (CaLCuV) activates the CP promoter in mesophyll and acts to derepress the promoter in vascular tissue, similar to that observed for Tomato golden mosaic virus (TGMV). Binding studies indicate that sequences mediating repression and activation of the TGMV and ...

journal_title：Virology

authors： Lacatus G,Sunter G

更新日期：2008-06-20 00:00:00

abstract：:Two types of dengue (DEN) 2 virus mutants were selected either by repeated exposure to acidic pH (acid mutant, AM), or by the addition of ammonium chloride to Aedes albopictus C6/36 cells prior to and during viral infection (fusion mutant, FM). Both mutants grew more slowly than the parent strain and induced smaller p...

journal_title：Virology

authors： Guirakhoo F,Hunt AR,Lewis JG,Roehrig JT

更新日期：1993-05-01 00:00:00

abstract：:A nonhemagglutinating mutant of a 1978 isolate of canine parvovirus (CPV) was derived after repeated passages in the NLFK feline kidney cell line. The mutant CPV was antigenically indistinguishable from wild-type virus when tested with 82 monoclonal antibodies, and it replicated in cat and dog cell lines in culture. S...

journal_title：Virology

authors： Parrish CR,Burtonboy G,Carmichael LE

更新日期：1988-03-01 00:00:00

abstract：:The molecular cloning of the DNA provirus of Mason-Pfizer monkey virus (M-PMV) is described. Fourteen independent clones of integrated M-PMV proviruses were isolated from a human embryo kidney cell line that had been previously derived from a single cell clone infected with M-PMV. Characterization of these clones for ...

journal_title：Virology

authors： Barker CS,Pickel J,Tainsky M,Hunter E

更新日期：1986-09-01 00:00:00

abstract：:Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the ...

journal_title：Virology

authors： Perkus ME,Goebel SJ,Davis SW,Johnson GP,Norton EK,Paoletti E

更新日期：1991-01-01 00:00:00

abstract：:Loss of T cell homeostasis usually precedes the onset of AIDS. We hypothesized that rapid progressors may be transmitted with HIV-1 that is particularly able to perturb T cell homeostasis. To this end, we have tested two transmitted, syncytium-inducing (SI) viral isolates from a rapid progressor in two thymus models. ...

journal_title：Virology

authors： Meissner EG,Duus KM,Gao F,Yu XF,Su L

更新日期：2004-10-10 00:00:00

abstract：:The expression of cytokines may influence the development of lymphoma in retrovirally infected animals in at least two ways: (1) cytokines in the tumor environment may stimulate the proliferation of tumor cells and/or (2) cytokines in the tumor environment may diminish the cell-mediated antitumor immune response. To e...

journal_title：Virology

authors： Beaty RM,Rulli K,Bost KL,Pantginis J,Lenz J,Levy LS

更新日期：1999-09-01 00:00:00

abstract：:Expression of foreign peptides on the surface of cowpea mosaic virus particles leads to the creation of chimaeras with a variety of phenotypes and yields. Two factors were shown to be particularly significant in determining the properties of a given chimaera: the length of the inserted sequence and its isoelectric poi...

journal_title：Virology

authors： Porta C,Spall VE,Findlay KC,Gergerich RC,Farrance CE,Lomonossoff GP

更新日期：2003-05-25 00:00:00

abstract：:In this study, we analyzed the replication and budding sites of severe acute respiratory syndrome coronavirus (SARS-CoV) at early time points of infection. We detected cytoplasmic accumulations containing the viral nucleocapsid protein, viral RNA and the non-structural protein nsp3. Using EM techniques, we found that ...

journal_title：Virology

authors： Stertz S,Reichelt M,Spiegel M,Kuri T,Martínez-Sobrido L,García-Sastre A,Weber F,Kochs G

更新日期：2007-05-10 00:00:00

abstract：:The sequence of Broadhaven (BRD) virus segment 5, the major genetic determinant of serotype, is 1658 nucleotides in length and contains a single open reading frame (ORF) having the coding capacity for a protein of Mr 52.5K. Comparison of the ORF of segment 5 of BRD virus with published sequences of bluetongue virus (B...

journal_title：Virology

authors： Moss SR,Fukusho A,Nuttall PA

更新日期：1990-11-01 00:00:00

abstract：:The mitotic spindle assembly checkpoint (SAC) ensures faithful chromosome segregation during mitosis by censoring kinetochore-microtubule interactions. It is frequently rendered dysfunctional during carcinogenesis causing chromosome missegregation and genomic instability. There are conflicting reports whether the HPV1...

journal_title：Virology

authors： Yu Y,Munger K

更新日期：2012-10-10 00:00:00

abstract：:Unusual Plum pox virus (PPV) isolates (named Tat isolates) were discovered on sour cherry (Prunus cerasus) in Russia. They failed to be recognized by RT-PCR using commonly employed primers specific to the strains C or CR (the only ones that proved able to infect sour cherry) as well as to the strains M and W. Some of ...

journal_title：Virology

authors： Chirkov S,Ivanov P,Sheveleva A,Zakubanskiy A,Osipov G

更新日期：2017-02-01 00:00:00

abstract：:Avarol is a sesquiterpenoid hydroquinone, which displays no inhibitory potencies on mammalian DNA polymerases alpha, beta, and gamma, on mammalian RNA polymerases I, II, and III, or on reverse transcriptases from Moloney murine leukemia virus (Mo-MuLV) and from HIV. For a further elucidation of the antiviral effect of...

journal_title：Virology

authors： Kuchino Y,Nishimura S,Schröder HC,Rottmann M,Müller WE

更新日期：1988-08-01 00:00:00

abstract：:Within immunocompromised populations, the JC polyomavirus is the cause of the often-fatal disease Progressive Multifocal Leukoencephalopathy (PML). JC virus encodes a protein, termed T-antigen (T-ag), which is essential for its replication and pathogenicity. Previous studies of JCV T-ag have, in general, used antibodi...

journal_title：Virology

authors： Grubman SA,Shin J,Phelan PJ,Gong A,Can H,Dilworth R,Kini SK,Gagnon D,Archambault J,Meinke G,Bohm A,Jefferson DM,Bullock PA

更新日期：2016-10-01 00:00:00

abstract：:While trying to generate a site-directed deletion in the ORF63 latency-associated gene of varicella-zoster virus (VZV) Oka, we constructed a virus with an unexpected rearrangement. The virus has a small deletion in both copies of ORF63 and two copies of a cassette inserted between ORFs 64/65 and 68/69 containing (a) t...

journal_title：Virology

authors： Ambagala AP,Krogmann T,Qin J,Pesnicak L,Cohen JI

更新日期：2010-04-10 00:00:00

abstract：:The role of the nucleocapsid protein of HIV-1 Gag in virus assembly was investigated using Gag truncation mutants, a nucleocapsid deletion mutant, and point mutations in the nucleocapsid region of Gag, in transfected COS cells, and in stable T-cell lines. Consistent with previous investigations, a truncation containin...

journal_title：Virology

authors： Dawson L,Yu XF

更新日期：1998-11-10 00:00:00

abstract：:Growth of clinical specimens of influenza viruses in eggs can result in the selection of antigenic variants distinct from corresponding viruses grown in mammalian tissue culture. To evaluate the contribution of host cell selection on the antigenic diversity of human influenza isolates, as seen in annual surveillance s...

journal_title：Virology

authors： Meyer WJ,Wood JM,Major D,Robertson JS,Webster RG,Katz JM

更新日期：1993-09-01 00:00:00