Abstract:
:Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the left and the right termini of the vaccinia genome. We report here the engineered deletion within a single vaccinia virus of 32.7 kb of DNA (including 38 ORFS) from the left terminus and 14.9 kb of DNA (including 17 ORFS) from the right terminus.
journal_name
Virologyjournal_title
Virologyauthors
Perkus ME,Goebel SJ,Davis SW,Johnson GP,Norton EK,Paoletti Edoi
10.1016/0042-6822(91)90047-fsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
406-10issue
1eissn
0042-6822issn
1096-0341journal_volume
180pub_type
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