Deletion of 55 open reading frames from the termini of vaccinia virus.

Abstract:

:Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the left and the right termini of the vaccinia genome. We report here the engineered deletion within a single vaccinia virus of 32.7 kb of DNA (including 38 ORFS) from the left terminus and 14.9 kb of DNA (including 17 ORFS) from the right terminus.

journal_name

Virology

journal_title

Virology

authors

Perkus ME,Goebel SJ,Davis SW,Johnson GP,Norton EK,Paoletti E

doi

10.1016/0042-6822(91)90047-f

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

406-10

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

180

pub_type

杂志文章

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