Abstract:
:Expression of foreign peptides on the surface of cowpea mosaic virus particles leads to the creation of chimaeras with a variety of phenotypes and yields. Two factors were shown to be particularly significant in determining the properties of a given chimaera: the length of the inserted sequence and its isoelectric point. The deleterious effect of high isoelectric point on the ability of chimeras to produce a systemic infection occurs irrespective of the site of insertion of the peptide. Ultrastructural analysis of tissue infected with chimaeras with different phenotypes showed that all produced particles with a tendency to aggregate, irrespective of the size or isoelectric point of the insert. Host range and transmission studies revealed that the expression of a foreign peptide did not (1) alter the virus host range, (2) increase the rate of transmission by beetles or through seed, or (3) change the insect vector specificity. These findings have implications for both the utility and the biosafety of Cowpea mosaic virus-based chimaeras.
journal_name
Virologyjournal_title
Virologyauthors
Porta C,Spall VE,Findlay KC,Gergerich RC,Farrance CE,Lomonossoff GPdoi
10.1016/s0042-6822(03)00140-5subject
Has Abstractpub_date
2003-05-25 00:00:00pages
50-63issue
1eissn
0042-6822issn
1096-0341pii
S0042682203001405journal_volume
310pub_type
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