Cowpea mosaic virus-based chimaeras. Effects of inserted peptides on the phenotype, host range, and transmissibility of the modified viruses.


:Expression of foreign peptides on the surface of cowpea mosaic virus particles leads to the creation of chimaeras with a variety of phenotypes and yields. Two factors were shown to be particularly significant in determining the properties of a given chimaera: the length of the inserted sequence and its isoelectric point. The deleterious effect of high isoelectric point on the ability of chimeras to produce a systemic infection occurs irrespective of the site of insertion of the peptide. Ultrastructural analysis of tissue infected with chimaeras with different phenotypes showed that all produced particles with a tendency to aggregate, irrespective of the size or isoelectric point of the insert. Host range and transmission studies revealed that the expression of a foreign peptide did not (1) alter the virus host range, (2) increase the rate of transmission by beetles or through seed, or (3) change the insect vector specificity. These findings have implications for both the utility and the biosafety of Cowpea mosaic virus-based chimaeras.






Porta C,Spall VE,Findlay KC,Gergerich RC,Farrance CE,Lomonossoff GP




Has Abstract


2003-05-25 00:00:00
















  • Mapping of the tobacco vein mottling virus VPg cistron.

    abstract::The location of the cistron encoding the genome-linked protein (VPg) in the potyvirus tobacco vein mottling virus (TVMV) was investigated. Precipitation of 125I-labeled VPg with anti-tobacco etch virus 49K nuclear inclusion protein antiserum (which reacts with the NIa nuclear inclusion protein of TVMV) indicated that ...


    pub_type: 杂志文章


    authors: Shahabuddin M,Shaw JG,Rhoads RE

    更新日期:1988-04-01 00:00:00

  • Development of plaque assays for hepatitis C virus-JFH1 strain and isolation of mutants with enhanced cytopathogenicity and replication capacity.

    abstract::HCV culture in vitro results in massive cell death, which suggests the presence of HCV-induced cytopathic effects. Therefore, we investigated its mechanisms and viral nucleotide sequences involved in this effect using HCV-JFH1 cell culture and a newly developed HCV plaque assay technique. The plaque assay developed cy...


    pub_type: 杂志文章


    authors: Sekine-Osajima Y,Sakamoto N,Mishima K,Nakagawa M,Itsui Y,Tasaka M,Nishimura-Sakurai Y,Chen CH,Kanai T,Tsuchiya K,Wakita T,Enomoto N,Watanabe M

    更新日期:2008-02-05 00:00:00

  • In vivo priming and activation of memory cytotoxic T-lymphocytes (CTL) by a chimeric simian virus 40 T antigen expressing an eight amino acid residue herpes simplex virus gB CTL epitope.

    abstract::The simian virus 40 (SV40) large T antigen was used as an immunogenic vector to express a herpes simplex virus type 1 (HSV-1) glycoprotein B (gB), H-2Kb-restricted cytotoxic T lymphocyte (CTL) recognition epitope corresponding to amino acid residues 498-505. Immunization of naive, C57BL/6 mice with a cell line, B6/350...


    pub_type: 杂志文章


    authors: Bonneau RH,Fu TM,Tevethia SS

    更新日期:1993-12-01 00:00:00

  • The nucleotide sequence of apple stem grooving capillovirus genome.

    abstract::The complete nucleotide sequence of apple stem grooving virus (ASGV) genome has been determined. The genome is 6496 nucleotides in length excluding a 3'-terminal poly(A) tail and contains two overlapping open reading frames (ORFs). ORF1 begins at nucleotide position 37 and is terminated at position 6341, encoding a pr...


    pub_type: 杂志文章


    authors: Yoshikawa N,Sasaki E,Kato M,Takahashi T

    更新日期:1992-11-01 00:00:00

  • Histamine enhances HIV-1-induced modulation of dendritic cells to skew naïve T cell differentiation toward regulatory T cells.

    abstract::Altered cytokine profiles and imbalanced frequencies of CD4(+) T helper cell subsets are thought to be linked with HIV-1/AIDS pathogenesis, but the causes need to be further clarified. Histamine, a biogenic amine with many functions, shows enhancement in HIV-1 infected individuals, which are considered to link with di...


    pub_type: 杂志文章


    authors: Zhai RR,Jiang AP,Wang HB,Ma L,Ren XX,Jiang JF,Wu L,Wei JF,Wang JH

    更新日期:2013-08-01 00:00:00

  • Preliminary in vitro growth cycle and transmission studies of HIV-1 in an autologous primary cell assay of blood-derived macrophages and peripheral blood mononuclear cells.

    abstract::Recent interest focused on the dynamics of HIV-1 replication in primary monocytes/macrophages and T-lymphocytes of the immune system, as well as the standardization of virological and immunological in vitro assays with primary isolates, provided the impetus for these studies. These types of studies have never been per...


    pub_type: 杂志文章


    authors: Tsai WP,Conley SR,Kung HF,Garrity RR,Nara PL

    更新日期:1996-12-15 00:00:00

  • Characterization of the integration site of the CMV mtr in a tumor cell line.

    abstract::Previous studies have shown that a 558-bp fragment of human cytomegalovirus (CMV) DNA contained within pCM4127 and designated CMV mtr can morphologically transform rodents cells in vitro. By cotransformation with pCM4127 and a plasmid conferring G418 resistance, pSV2neo, morphologically transformed NIH3T3 cell lines w...


    pub_type: 杂志文章


    authors: Buonaguro FM,McDougall JK,Galloway DA

    更新日期:1987-01-01 00:00:00

  • U937 cells overexpressing bcl-xl are resistant to human immunodeficiency virus-1-induced apoptosis and human immunodeficiency virus-1 replication.

    abstract::Many viruses, including human immunodeficiency virus type 1 (HIV-1), induce apoptosis and are affected by cellular expression of antiapoptotic genes. We sought to examine the effect of antiapoptotic gene expression on HIV replication by transfecting the promyelomonocytic cell line U937 with the bcl-xl gene to obtain c...


    pub_type: 杂志文章


    authors: Marshall WL,Datta R,Hanify K,Teng E,Finberg RW

    更新日期:1999-03-30 00:00:00

  • Studies on the attenuation phenotype of polio vaccines: poliovirus RNA polymerase derived from Sabin type 1 sequence is temperature sensitive in the uridylylation of VPg.

    abstract::Determinants of temperature sensitivity and/or attenuation in Sabin type 1 poliovirus reside in the 5' NTR and coding sequences of the capsid proteins and viral RNA polymerase, 3D(pol). Previous studies have implicated at least two mutations in 3D(pol) of Sabin 1 vaccine strain [PV1(S)], including a Y73H change, as co...


    pub_type: 杂志文章


    authors: Paul AV,Mugavero J,Yin J,Hobson S,Schultz S,van Boom JH,Wimmer E

    更新日期:2000-06-20 00:00:00

  • In vitro exposure to highly cytopathic HIV-1 X4 strains increases expression of mucosa-associated integrins on CD4(+) T cells.

    abstract::To determine whether infection with HIV-1 strains of different tropisms would influence expression of the mucosa-associated integrins alpha 4 beta 7 and alpha E beta 7 or the lymph node homing receptor L-selectin on peripheral T lymphocytes, cells were infected with the CXCR4-tropic (X4)/syncytium-inducing (SI) HIV-1(...


    pub_type: 杂志文章


    authors: Cummins JE Jr,Bunn WJ,Hall SD,Donze HH,Mestecky J,Jackson S

    更新日期:2001-02-15 00:00:00

  • Lack of LTR and ENV genetic variation during bovine leukemia virus-induced leukemogenesis.

    abstract::Genetic variation of the Bovine Leukemia Virus (BLV) appears to be limited in vitro and during the latent phase of the disease. However, cells in tumors often harbor deleted proviruses that are defective for expression. In order to gain insight into the involvement of viral genetic variation during pathogenesis, the B...


    pub_type: 杂志文章


    authors: Willems L,Kerkhofs P,Burny A,Mammerickx M,Kettmann R

    更新日期:1995-01-10 00:00:00

  • The herpes simplex virus type 2 equivalent of the herpes simplex virus type 1 US7 gene and its flanking sequences.

    abstract::Nucleotide sequencing studies (D. J. McGeoch, A. Dolan, S. Donald, and F. Rixon, 1985, J. Mol. Biol. 181, 1-14) have indicated that herpes simplex virus type 1 (HSV-1) has a coding sequence, referred to as US7, between the genes for the glycoproteins D and E (gD and gE). Northern blot analysis and nucleotide sequencin...


    pub_type: 杂志文章


    authors: Hodgman TC,Minson AC

    更新日期:1986-08-01 00:00:00

  • Down-regulation of the internal ribosome entry site (IRES)-mediated translation of the hepatitis C virus: critical role of binding of the stem-loop IIId domain of IRES and the viral core protein.

    abstract::In a previous study, we observed that hepatitis C virus (HCV) core protein specifically inhibits translation initiated by an HCV internal ribosome entry site (IRES). To investigate the mechanism by which down-regulation of HCV translation occurs, a series of mutations were introduced into the IRES element, as well as ...


    pub_type: 杂志文章


    authors: Shimoike T,Koyama C,Murakami K,Suzuki R,Matsuura Y,Miyamura T,Suzuki T

    更新日期:2006-02-20 00:00:00

  • Gut microbiota is associated with protection against Marek's disease virus infection in chickens.

    abstract::Marek's Disease Virus (MDV) infects chickens via respiratory route and causes lymphomas in internal organs including gastrointestinal tract. MDV infection causes a shift in the gut microbiota composition. However, interactions between the gut microbiota and immune responses against MDV infection are not well understoo...


    pub_type: 杂志文章


    authors: Bavananthasivam J,Astill J,Matsuyama-Kato A,Taha-Abdelaziz K,Shojadoost B,Sharif S

    更新日期:2021-01-15 00:00:00

  • Protein composition of the vaccinia virus mature virion.

    abstract::The protein content of vaccinia virus mature virions, purified by rate zonal and isopycnic centrifugations and solubilized by SDS or a solution of urea and thiourea, was determined by the accurate mass and time tag technology which uses both tandem mass spectrometry and Fourier transform-ion cyclotron resonance mass s...


    pub_type: 杂志文章


    authors: Resch W,Hixson KK,Moore RJ,Lipton MS,Moss B

    更新日期:2007-02-05 00:00:00

  • The role of nucleocapsid of HIV-1 in virus assembly.

    abstract::The role of the nucleocapsid protein of HIV-1 Gag in virus assembly was investigated using Gag truncation mutants, a nucleocapsid deletion mutant, and point mutations in the nucleocapsid region of Gag, in transfected COS cells, and in stable T-cell lines. Consistent with previous investigations, a truncation containin...


    pub_type: 杂志文章


    authors: Dawson L,Yu XF

    更新日期:1998-11-10 00:00:00

  • HIV-1 inactivation by 4-vinylpyridine is enhanced by dissociating Zn(2+) from nucleocapsid protein.

    abstract::Selective inactivation of critical cysteine residues in human immunodeficiency virus type one (HIV-1) was observed after treatment with 4-vinylpyridine (4-VP), with and without the membrane-permeable metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN). Chromatographic analysis showed that cystein...


    pub_type: 杂志文章


    authors: Morcock DR,Thomas JA,Sowder RC 2nd,Henderson LE,Crise BJ,Gorelick RJ

    更新日期:2008-05-25 00:00:00

  • Polyomavirus hr-t mutant-specific induction of a G2/M cell-cycle arrest that is not overcome by the expression of middle T and/or small T.

    abstract::The polyomavirus hr-t class of mutants has served as a major prototype to study the function of middle T + small T in the virus lytic cycle, Biochem. Biophys. Acta 695 (2), 69-95). The properties of these middle T + small T defective mutants were defined by comparisons with "wild-type" strains reconstructed by marker ...


    pub_type: 杂志文章


    authors: Spink KM,Fluck MM

    更新日期:2003-03-15 00:00:00

  • Induction of a protective antibody response to foot and mouth disease virus in mice following oral or parenteral immunization with alfalfa transgenic plants expressing the viral structural protein VP1.

    abstract::The utilization of transgenic plants expressing recombinant antigens to be used in the formulation of experimental immunogens has been recently communicated. We report here the development of transgenic plants of alfalfa expressing the structural protein VP1 of foot and mouth disease virus (FMDV). The presence of the ...


    pub_type: 杂志文章


    authors: Wigdorovitz A,Carrillo C,Dus Santos MJ,Trono K,Peralta A,Gómez MC,Ríos RD,Franzone PM,Sadir AM,Escribano JM,Borca MV

    更新日期:1999-03-15 00:00:00

  • Identification and characterization of RNA duplex unwinding and ATPase activities of an alphatetravirus superfamily 1 helicase.

    abstract::Dendrolimus punctatus tetravirus (DpTV) belongs to the genus omegatetravirus of the Alphatetraviridae family. Sequence analysis predicts that DpTV replicase contains a putative helicase domain (Hel). However, the helicase activity in alphatetraviruses has never been formally determined. In this study, we determined th...


    pub_type: 杂志文章


    authors: Wang Q,Han Y,Qiu Y,Zhang S,Tang F,Wang Y,Zhang J,Hu Y,Zhou X

    更新日期:2012-11-25 00:00:00

  • Identification of major antigenic domains of duck hepatitis B virus pre-S protein by peptide scanning.

    abstract::Neutralization epitopes of duck hepatitis B virus (DHBV) have been previously mapped within the N-terminal portion of the pre-S protein using monoclonal antibodies. However, the immune response of ducks to this region is not well characterized at the amino acid level. To this end, we have immunized adult Pekin ducks w...


    pub_type: 杂志文章


    authors: Chassot S,Lambert V,Kay A,Godinot C,Trepo C,Cova L

    更新日期:1994-04-01 00:00:00

  • The genome-linked protein of cowpea mosaic virus is coded by RNA from the bottom component.

    abstract::It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...


    pub_type: 杂志文章


    authors: Stanley J,Goldbach R,Van Kammen A

    更新日期:1980-10-15 00:00:00

  • Virion-associated viral fibroblast growth factor stimulates cell motility.

    abstract::The Autographa californica M nucleopolyhedrovirus (AcMNPV) viral fibroblast growth factor (vFGF) has functional parallels to cellular FGFs. Deletion of the AcMNPV vfgf has no obvious phenotype in cell culture but delays the time of insect death. Here, we determined vFGF production during virus infection. vFGF was dete...


    pub_type: 杂志文章


    authors: Lehiy CJ,Martinez O,Passarelli AL

    更新日期:2009-12-05 00:00:00

  • Isolation of mutations of the phage Mu ner gene.

    abstract::A method was devised which allows the easy detection of mutations within the ner gene of Mu DNA. This method is based upon the observation that a transcriptional gene A-galK fusion containing the complete ner gene and the cts62 allele does not express the galK gene in an Escherichia coli strain lacking functional inte...


    pub_type: 杂志文章


    authors: König U,Kirschner P,Schumann W

    更新日期:1988-05-01 00:00:00

  • Specific cell surface requirements for the infection of CD4-positive cells by human immunodeficiency virus types 1 and 2 and by Simian immunodeficiency virus.

    abstract::Human CD4 was expressed on a range of mammalian cell lines. CD4+ non-primate cells, derived from rat, hamster, mink, cat, and rabbit, bind recombinant gp120 of human immunodeficiency virus type 1 (HIV-1) but are resistant to HIV-1 infection. CD4 expression on various human, rhesus, and African green monkey cell lines ...


    pub_type: 杂志文章


    authors: Clapham PR,Blanc D,Weiss RA

    更新日期:1991-04-01 00:00:00

  • Nucleotide sequence of the leader and nucleocapsid protein gene of mumps virus and epitope mapping with the in vitro expressed nucleocapsid protein.

    abstract::The nucleotide sequence of the leader and the gene encoding the nucleocapsid protein (NP) of mumps virus Miyahara strain have been determined. The leader sequence is 55 nucleotides in length and the NP gene is 1845 nucleotides in length, exclusive of poly(A). The NP gene codes for a protein of 549 amino acids, with a ...


    pub_type: 杂志文章


    authors: Tanabayashi K,Takeuchi K,Hishiyama M,Yamada A,Tsurudome M,Ito Y,Sugiura A

    更新日期:1990-07-01 00:00:00

  • Structure of the gene encoding the M1 protein of sonchus yellow net virus.

    abstract::The gene encoding the M1 protein of sonchus yellow net virus (SYNV), a plant rhabdovirus, has been sequenced and identified by Western blot analysis of SYNV proteins using antibodies directed against a fusion protein derived from a portion of the sequenced gene. The M1 gene is positioned between nucleotides 4039 and 5...


    pub_type: 杂志文章


    authors: Hillman BI,Heaton LA,Hunter BG,Modrell B,Jackson AO

    更新日期:1990-11-01 00:00:00

  • Phosphoprotein NS of vesicular stomatitis virus: phosphorylated states and transcriptional activities of intracellular and virion forms.

    abstract::The phosphorylation and transcriptional competence of the free cytoplasmic form and the virion form of NS protein of vesicular stomatitis virus (VSV-Indiana/Mudd-Summers) were compared. NS protein is known to exist in two distinct phosphorylated states, NS1 and NS2, that are resolvable by gel electrophoresis. In vitro...


    pub_type: 杂志文章


    authors: Masters PS,Banerjee AK

    更新日期:1986-10-30 00:00:00

  • Efficient expression of foreign proteins in roots from tobravirus vectors.

    abstract::Viral vectors were constructed from infectious cDNA clones of each of the three tobraviruses, tobacco rattle virus (TRV), pea early-browning virus (PEBV), and pepper ringspot virus (PepRSV). RNA2 of each of the three viruses was modified to carry an additional coat protein subgenomic promoter and was used to express g...


    pub_type: 杂志文章


    authors: MacFarlane SA,Popovich AH

    更新日期:2000-02-01 00:00:00

  • Functional expression of influenza A viral nucleoprotein in cells transformed with cloned DNA.

    abstract::Simian cells permissive for influenza A virus infection were stably transformed with a full-length cloned influenza A nucleoprotein gene under the control of an inducible metallothionein promoter and linked to a dihydrofolate reductase gene to facilitate cell selection. Transformed cells synthesized a virus-specific n...


    pub_type: 杂志文章


    authors: Ryan KW,Mackow ER,Chanock RM,Lai CJ

    更新日期:1986-10-15 00:00:00