Cowpea mosaic virus-based chimaeras. Effects of inserted peptides on the phenotype, host range, and transmissibility of the modified viruses.

Abstract:

:Expression of foreign peptides on the surface of cowpea mosaic virus particles leads to the creation of chimaeras with a variety of phenotypes and yields. Two factors were shown to be particularly significant in determining the properties of a given chimaera: the length of the inserted sequence and its isoelectric point. The deleterious effect of high isoelectric point on the ability of chimeras to produce a systemic infection occurs irrespective of the site of insertion of the peptide. Ultrastructural analysis of tissue infected with chimaeras with different phenotypes showed that all produced particles with a tendency to aggregate, irrespective of the size or isoelectric point of the insert. Host range and transmission studies revealed that the expression of a foreign peptide did not (1) alter the virus host range, (2) increase the rate of transmission by beetles or through seed, or (3) change the insect vector specificity. These findings have implications for both the utility and the biosafety of Cowpea mosaic virus-based chimaeras.

journal_name

Virology

journal_title

Virology

authors

Porta C,Spall VE,Findlay KC,Gergerich RC,Farrance CE,Lomonossoff GP

doi

10.1016/s0042-6822(03)00140-5

subject

Has Abstract

pub_date

2003-05-25 00:00:00

pages

50-63

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042682203001405

journal_volume

310

pub_type

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