Abstract:
:Little is known about the neutralization properties of HIV-1 in India to optimally design and test vaccines. For this reason, a functional Env clone was obtained from each of ten newly acquired, heterosexually transmitted HIV-1 infections in Pune, Maharashtra. These clones formed a phylogenetically distinct genetic lineage within subtype C. As Env-pseudotyped viruses the clones were mostly resistant to IgG1b12, 2G12 and 2F5 but all were sensitive to 4E10. When compared to a large multi-subtype panel of Env-pseudotyped viruses (subtypes B, C and CRF02_AG) in neutralization assays with a multi-subtype panel of HIV-1-positive plasma samples, the Indian Envs were remarkably complex. With the exception of the Indian Envs, results of a hierarchical clustering analysis showed a strong subtype association with the patterns of neutralization susceptibility. From these patterns we were able to identify 19 neutralization cluster-associated amino acid signatures in gp120 and 14 signatures in the ectodomain and cytoplasmic tail of gp41. We conclude that newly transmitted Indian Envs are antigenically complex in spite of close genetic similarity. Delineation of neutralization-associated amino acid signatures provides a deeper understanding of the antigenic structure of HIV-1 Env.
journal_name
Virologyjournal_title
Virologyauthors
Kulkarni SS,Lapedes A,Tang H,Gnanakaran S,Daniels MG,Zhang M,Bhattacharya T,Li M,Polonis VR,McCutchan FE,Morris L,Ellenberger D,Butera ST,Bollinger RC,Korber BT,Paranjape RS,Montefiori DCdoi
10.1016/j.virol.2008.12.032subject
Has Abstractpub_date
2009-03-15 00:00:00pages
505-20issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(08)00845-3journal_volume
385pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::GV1B is one of five viroids that have recently been purified from grapevines. GV1B has now been sequenced and its 363 nucleotide residues can potentially form the typical rod-like structure of viroids with 67% of nucleotides base-paired. GV1B has highest sequence similarity with grapevine yellow speckle viroid (GYSV; ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90450-9
更新日期:1989-06-01 00:00:00
abstract::This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90172-4
更新日期:1989-04-01 00:00:00
abstract::A series of clones has been derived from an interferon-resistant murine cell line, Ltk- aprt-, and their antiviral properties have been characterized. In the parental Ltk- aprt- line interferon is unable to establish antiviral properties or to increase the levels of 2,5-oligo(A) synthetase, the 2,5-oligo(A)-activated ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90400-x
更新日期:1988-01-01 00:00:00
abstract::Hepatitis B virus-infected patients frequently have viral particles with DNA derived from differently spliced RNA. Which factors influence the synthesis of these splice variants is unclear. We analysed the type of splice variants produced from different genotypes and determined whether they are secreted as efficiently...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0331
更新日期:2000-06-05 00:00:00
abstract::Ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (HSV-2) DNA sequences. Two vulvar tumors and one vulvar dysplastic tissue were found to contain DNA sequences homologous to the BglII O fragment (coordinates...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90179-0
更新日期:1986-11-01 00:00:00
abstract::The 50-70 S RNA complex of a clone of Kirsten murine leukemia virus (Ki-MLV), propagated in NIH 3T3 cells, as well as that of a Moloney (Mo)-MLV, propagated in a mouse thymus-bone marrow cell line, contained an approximately 30 S RNA of unknown biological activity in addition to 38 S genomic RNA. The approximately 30 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90225-2
更新日期:1977-11-01 00:00:00
abstract::Sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (HN) protein of a 1957 isolate of parainfluenza type 3 virus (PIV3) were produced and used to examine antigenic variation in clinical strains. Analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90395-2
更新日期:1985-06-01 00:00:00
abstract::Beet necrotic yellow vein virus (BNYVV) has a quadripartite plus-strand RNA genome in which the two smallest genome components, RNA 3 and 4, are not necessary for virus multiplication in leaves. Infectious transcripts of BNYVV RNA 3 and 4 have already been described (V. Ziegler-Graff, S. Bouzoubaa, I. Jupin, H. Guille...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90131-1
更新日期:1989-09-01 00:00:00
abstract::Tetherin has been defined as a restriction factor of HIV-1 and several other enveloped viruses. However, the significance of tetherin in viral infection remains to be further addressed. Here, we investigated whether tetherin plays a role in HSV-2 infection. Our study revealed that overexpression of tetherin restricted...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.11.005
更新日期:2015-01-15 00:00:00
abstract::Measles virus V protein represses genome replication through a poorly understood mechanism, which led us to investigate whether V protein might be an RNA-binding modulatory factor. Recombinant V protein, expressed from transfected HEp-2 cells or E. coli, formed protein-RNA complexes with poly-guanosine (poly-G) or pol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.018
更新日期:2006-04-25 00:00:00
abstract::To illuminate the molecular basis for host range restriction of parainfluenza virus replication, we have examined the types of virus macromolecules produced during abortive infection of nonpermissive MDBK cells with human parainfluenza virus type 1 (hPIV1). While these cells do not support production of hPIV1 virus, t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0287
更新日期:1996-06-01 00:00:00
abstract::Human cytomegalovirus (HCMV) can be isolated from peripheral blood leukocytes; however, in vitro, only abortive infection of monocytes, lymphocytes, and granulocytes has been detected. These studies demonstrate that freshly isolated monocytes can be infected with HCMV. Infection of monocytes was not associated with lo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1383
更新日期:1993-08-01 00:00:00
abstract::We examined the kinetics of membrane fusion of wild type (wt) and Delta6K mutant Semliki Forest virus in a liposomal model system. The final extent of membrane fusion of the mutant (at pH 5.5) was approximately one third that of the wt virus, although the level of E1 (fusion protein) trimerization was, in fact, greate...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.04.043
更新日期:2004-08-01 00:00:00
abstract::The 24,000-molecular-weight major internal protein (p24) and the 15,000-molecular-weight nucleic acid binding protein (p15) of human T-cell leukemia virus type II (HTLV-II) were subjected to amino acid composition and amino-terminal amino acid sequence analysis. A comparison of amino acid composition of p24 and p15 of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90434-9
更新日期:1985-04-15 00:00:00
abstract::In the present study, 10 clinical isolates of dengue virus were selected according to their susceptibility to the inhibitory effect of nitric oxide radical, NO. Five of them are nitric oxide-susceptible viruses while the other five are nitric oxide-resistant viruses. These isolates were investigated to identify geneti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.030
更新日期:2008-07-05 00:00:00
abstract::Unusual Plum pox virus (PPV) isolates (named Tat isolates) were discovered on sour cherry (Prunus cerasus) in Russia. They failed to be recognized by RT-PCR using commonly employed primers specific to the strains C or CR (the only ones that proved able to infect sour cherry) as well as to the strains M and W. Some of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.12.016
更新日期:2017-02-01 00:00:00
abstract::HIV-1 Nef is expressed in astrocytes, but a contribution to neuropathogenesis and the development of HIV-associated dementia (HAD) remains uncertain. To determine the neuropathogenic actions of the HIV-1 Nef protein, the brain-derived (YU-2) and blood-derived (NL4-3) Nef proteins were expressed in neural cells using a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.08.024
更新日期:2004-11-24 00:00:00
abstract::Chimeric proteins consisting of the VP2 capsid protein of human parvovirus B19 and defined linear epitopes from human herpes simplex virus type 1 and mouse hepatitis virus A59 inserted at the N-terminus and at a predicted surface region were expressed by recombinant baculoviruses. The chimeric proteins expressed the i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1059
更新日期:1994-02-01 00:00:00
abstract::HIV-1 infection leads to impaired antigen-specific T cell proliferation, increased susceptibility of T cells to apoptosis, progressive impairment of T-helper 1 (Th1) responses, and altered maturation of HIV-1-specific memory cells. We have identified similar impairments in HIV-1 transgenic (Tg) rats. Tg rats developed...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.010
更新日期:2004-03-30 00:00:00
abstract::Enveloped virus entry occurs when viral and cellular membranes fuse releasing particle contents into the target cell. Human immunodeficiency virus (HIV) entry occurs by cell-free virus or virus transferred between infected and uninfected cells through structures called virological synapses. We developed a high-through...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.10.013
更新日期:2016-03-01 00:00:00
abstract::In our in vitro model for HPV16-mediated transformation, HPV16-immortalized human keratinocytes (HKc/HPV16) give rise to differentiation resistant, premalignant cells (HKc/DR). HKc/DR, but not HKc/HPV16, are resistant to growth inhibition by transforming growth factor beta (TGF-β), due to a partial loss of TGF-β recep...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.08.034
更新日期:2013-12-01 00:00:00
abstract::Nonspecific anti-inflammatory drugs have been purported to reduce the burden of severe influenza disease. We demonstrate that, unlike oseltamivir administration, simvastatin administration did not reduce morbidity, mortality, or viral load of mice infected with H1N1 or H5N1 viruses. No added benefit to the efficacy of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.01.017
更新日期:2013-04-25 00:00:00
abstract::Using a defective interfering Sendai virus stock (DIH4) freed of nondefective helper virus, we found that the closely related parainfluenza viruses 1 and 3 could substitute for the Sendai virus helper in replicating DIH4, creating chimeric nucleocapsids. The morbillivirus measles and the rhabdovirus VSV could not subs...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90660-4
更新日期:1991-05-01 00:00:00
abstract::Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies. Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215. Substitution of alanine for threonine at this position reduced early ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.10.002
更新日期:2009-01-05 00:00:00
abstract::Ovine pulmonary carcinoma, a contagious lung cancer of sheep, is caused by the oncogenic jaagsiekte sheep retrovirus (JSRV) that is closely related to a family of endogenous sheep retroviral sequences (ESRVs). By using exogenous virus-specific U3 oligonucleotide primers, the entire JSRV proviral genome or its 3' part ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9728
更新日期:1999-06-05 00:00:00
abstract::Following a bite from an infected tick, tick-borne flaviviruses cause encephalitis, meningitis and hemorrhagic fever in humans. Although these viruses spend most of their time in the tick, little is known regarding the virus-vector interactions. We developed a simple method for synchronously infecting Ixodes scapulari...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.057
更新日期:2007-09-01 00:00:00
abstract::Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In this paper, we demonstrate that p33 is phosphorylated in vivo and in vitro by ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.08.006
更新日期:2005-12-05 00:00:00
abstract::The technique of cDNA polymerase chain reaction was used to study the heterogeneity of adenovirus type 12 early region 1A mRNAs in infected and transformed cells. In addition to 13 S and 12 S mRNAs, three transcripts of 10 S, 9.5 S, and 9 S could be isolated from infected HeLa cells. A fourth transcript of 11 S was fo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90125-b
更新日期:1990-12-01 00:00:00
abstract::The ability of some Sendai virus stocks to strongly activate IFNbeta has long been known to be associated with defective-interfering (DI) genomes. We have compared SeV stocks containing various copyback and internal deletion DI genomes (and those containing only nondefective (ND) genomes) for their ability to activate...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.03.022
更新日期:2006-07-20 00:00:00
abstract::Oral-gastric delivery of vaccines is a preferred route of immunization and is particularly relevant to the development of vaccine-vector systems. We have investigated the ability of a replication deficient (E1-deleted) adenovirus construct (RAd68), which efficiently expresses the measles virus nucleocapsid (N) protein...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1281
更新日期:2002-02-15 00:00:00