当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Selective inhibition of formation of suppressor glutamine tRNA in Moloney murine leukemia virus-infected NIH-3T3 cells by Avarol.

Selective inhibition of formation of suppressor glutamine tRNA in Moloney murine leukemia virus-infected NIH-3T3 cells by Avarol.

Abstract：

:Avarol is a sesquiterpenoid hydroquinone, which displays no inhibitory potencies on mammalian DNA polymerases alpha, beta, and gamma, on mammalian RNA polymerases I, II, and III, or on reverse transcriptases from Moloney murine leukemia virus (Mo-MuLV) and from HIV. For a further elucidation of the antiviral effect of Avarol, we used NIH-3T3 cells infected with Mo-MuLV as a model system. The results show that in uninfected NIH-3T3 cells Avarol (i) causes a 50% reduction of the growth rate only at the high concentration of 29.6 microM and (ii) is accumulated in the cytoplasm close to the nucleus. At the much lower concentrations of 1-3 microM, Avarol causes an almost complete inhibition of viral progeny release. Moreover, it is shown that at 3 microM Avarol, the increase of the Mo-MuLV-induced UAG suppressor glutamine tRNA (tRNA(UmUGGln) was reduced to the normal level. Dot blot hybridization studies revealed that Avarol displays no inhibitory activity on cellular and viral mRNA synthesis. Taking the processing pathway of viral polyprotein Pr180gag,pol to p80 (reverse transcriptase) as an example, our Western blotting experiments showed that the final maturation process, conversion of p110 to p80, is inhibited in Avarol-treated cells. From these data we conclude that Avarol prevents the suppression of the UAG termination codon at the gag-pol junction of the retroviral genome. The functional consequence of this event is very likely an inhibition of the readthrough of the retroviral protease gene which overlaps the pol and gag genes, resulting in the reduction of the protease synthesis which is necessary for the viral proliferation.

journal_name

Virology

journal_title

Virology

authors

Kuchino Y,Nishimura S,Schröder HC,Rottmann M,Müller WE

doi

10.1016/0042-6822(88)90596-x

subject

Has Abstract

pub_date

1988-08-01 00:00:00

pages

518-26

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

165

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Identification of Db- and Kb-restricted subdominant cytotoxic T-cell responses in lymphocytic choriomeningitis virus-infected mice.

    abstract：:Antiviral cytotoxic T-cells are critical for control of lymphocytic choriomeningitis virus (LCMV) infection in mice. In H-2b mice, the antiviral response is directed against three Db-restricted epitopes in the viral nucleoprotein (NP396-404) and glycoprotein (GP276-286 and GP33-41). Our present data revealed a clear h...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8934

    authors： van der Most RG,Murali-Krishna K,Whitton JL,Oseroff C,Alexander J,Southwood S,Sidney J,Chesnut RW,Sette A,Ahmed R

    更新日期：1998-01-05 00:00:00

  • Construction of recombinant avian infectious laryngotracheitis virus expressing the beta-galactosidase gene and DNA sequencing of the insertion region.

    abstract：:Avian infectious laryngotracheitis virus (ILTV), a herpesvirus, is a highly contagious pathogen that causes an upper respiratory tract infection in chickens. It is one of the major problems in the poultry industry worldwide. Current vaccines are not satisfactory due to the induction of latent infection. Here we descri...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1399

    authors： Guo P,Scholz E,Maloney B,Welniak E

    更新日期：1994-08-01 00:00:00

  • Molecular variability and genetic structure of the population of soybean mosaic virus based on the analysis of complete genome sequences.

    abstract：:The complete genomes of 30 Soybean mosaic virus (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutio...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.07.007

    authors： Seo JK,Ohshima K,Lee HG,Son M,Choi HS,Lee SH,Sohn SH,Kim KH

    更新日期：2009-10-10 00:00:00

  • Noninfectious L-particles supply functions which can facilitate infection by HSV-1.

    abstract：:The tegument of herpes simplex virus contains proteins important for the efficient initiation of infection. L-particles are noninfectious virion-related particles that lack the nucleocapsid but do contain tegument and envelope. Their ability to deliver functional proteins into cells was compared to that of virions by ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90906-6

    authors： McLauchlan J,Addison C,Craigie MC,Rixon FJ

    更新日期：1992-10-01 00:00:00

  • Identification of the nuclear localization and export signals of high risk HPV16 E7 oncoprotein.

    abstract：:The E7 oncoprotein of high risk human papillomavirus type 16 (HPV16) binds and inactivates the retinoblastoma (RB) family of proteins. Our previous studies suggested that HPV16 E7 enters the nucleus via a novel Ran-dependent pathway independent of the nuclear import receptors (Angeline, M., Merle, E., and Moroianu, J....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.09.037

    authors： Knapp AA,McManus PM,Bockstall K,Moroianu J

    更新日期：2009-01-05 00:00:00

  • Population dynamics studies of wild-type and drug-resistant mutant HIV in mixed infections.

    abstract：:We have studied the population dynamics in response to selective drug pressure of mixtures of wild-type and mutant HIV viruses exposed to either an inhibitor of the viral protease or a nonnucleoside allosteric inhibitor of the viral reverse transcriptase. In order to quantitate mutant virus present in a mixed populati...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8620

    authors： Rayner MM,Cordova B,Jackson DA

    更新日期：1997-09-15 00:00:00

  • Sequence similarity between the long terminal repeat coding regions of mammary-tumorigenic BALB/cV and renal-tumorigenic C3H-K strains of mouse mammary tumor virus.

    abstract：:The long terminal repeat (LTR) of mouse mammary tumor virus (MMTV) encodes a protein which functions as a superantigen. The BALB/cV strain differs from other exogenous MMTVs antigenically, biochemically, on the basis of restriction fragment analysis, and by the specificity of its superantigen for V beta 2+ T cells. In...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1480

    authors： Kang JJ,Schwegel T,Knepper JE

    更新日期：1993-09-01 00:00:00

  • Oligomerization and transport of the envelope protein of Moloney murine leukemia virus-TB and of ts1, a neurovirulent temperature-sensitive mutant of MoMuLV-TB.

    abstract：:Ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB (MoMuLV-TB), causes a progressive hindlimb paralytic disease in susceptible strains of mice. Previously, it has been shown that a single amino acid substitution, Val-25----Ile in gPr80env, is responsible for the temperature sensitivity, inefficien...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90438-h

    authors： Kamps CA,Lin YC,Wong PK

    更新日期：1991-10-01 00:00:00

  • The coat protein of beet curly top virus is essential for infectivity.

    abstract：:We have applied the procedure of Agrobacterium-mediated inoculation to develop a simple, efficient, and reproducible assay for the infectivity of the leafhopper-transmitted geminivirus, beet curly top virus (BCTV). This assay system was used to show that a coat protein mutant of BCTV is not infectious, but could be co...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90205-5

    authors： Briddon RW,Watts J,Markham PG,Stanley J

    更新日期：1989-10-01 00:00:00

  • Human immunodeficiency virus (HIV-1) infection selectively downregulates PD-1 expression in infected cells and protects the cells from early apoptosis in vitro and in vivo.

    abstract：:Programmed Death-1 (PD-1), a member of T cell costimulatory molecules is expressed in high levels on antigen specific T cells during chronic viral infection, whereas PD-1 expression in the context of HIV-1 infected CD4+ T cells is not known. Here we report that productively infected CD4+ T cells lose PD-1, whereas bys...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.03.015

    authors： Venkatachari NJ,Buchanan WG,Ayyavoo V

    更新日期：2008-06-20 00:00:00

  • The outer capsid protein VP4 of equine rotavirus strain H-2 represents a unique VP4 type by amino acid sequence analysis.

    abstract：:The nucleotide and deduced amino acid sequence of G serotype 3 equine rotavirus strain H-2 was determined. A predicted 776-amino-acid H-2 VP4 shows less than or equal to 85.3% identity to other rotavirus VP4 types sequenced to date and thus represents a new P serotype. A PCR-generated probe derived from a cDNA clone o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1152

    authors： Hardy ME,Gorziglia M,Woode GN

    更新日期：1993-03-01 00:00:00

  • 3Y1 rat cells are defective in processing of the envelope precursor protein of AKR virus.

    abstract：:Rat 3Y1 cells were infected by AKR virus through microinjection of molecularly cloned proviral DNA. Based on a strong immunofluorescence using anti-p30 as an antiserum a cell clone (RESA-2) was selected that had a high expression of viral antigens. Subsequent restriction analysis of its DNA revealed that the RESA-2 cl...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90361-6

    authors： van der Hoorn FA,Saris CJ,Bloemers HP

    更新日期：1983-01-30 00:00:00

  • Genetic identification of a portion of the herpes simplex virus ICP8 protein required for DNA-binding.

    abstract：:The major DNA-binding protein or infected cell protein 8 (ICP8) encoded by herpes simplex virus exhibits multiple interactions with the cell nucleus in that it interacts with the host cell nuclear matrix and viral DNA molecules as sequential stages in its maturational process (M. P. Quinlan, L. B. Chen, and D. M. Knip...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90272-3

    authors： Gao M,Bouchey J,Curtin K,Knipe DM

    更新日期：1988-04-01 00:00:00

  • Natural controlled HIV infection: preserved HIV-specific immunity despite undetectable replication competent virus.

    abstract：:Long-term non-progressive HIV infection, characterized by low but detectable viral load and stable CD4 counts in the absence of antiviral therapy, is observed in about 5% of HIV-infected patients. Here we identified four therapy naïve individuals who are strongly seropositive for HIV-1 but who lack evidence of detecta...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.06.001

    authors： Kloosterboer N,Groeneveld PH,Jansen CA,van der Vorst TJ,Koning F,Winkel CN,Duits AJ,Miedema F,van Baarle D,van Rij RP,Brinkman K,Schuitemaker H

    更新日期：2005-08-15 00:00:00

  • The V protein of the paramyxovirus SV5 interacts with damage-specific DNA binding protein.

    abstract：:The simian parainfluenza virus 5 (SV5) V/P gene encodes two proteins: V and the phosphoprotein P. The V and P proteins are amino coterminal for 164 residues, but they have unique carboxyl termini. The unique carboxyl terminus of V contains seven cysteine residues, resembles a zinc finger, and binds two atoms of zinc. ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9317

    authors： Lin GY,Paterson RG,Richardson CD,Lamb RA

    更新日期：1998-09-15 00:00:00

  • The role of proteolytic processing and the stable signal peptide in expression of the Old World arenavirus envelope glycoprotein ectodomain.

    abstract：:Maturation of the arenavirus GP precursor (GPC) involves proteolytic processing by cellular signal peptidase and the proprotein convertase subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P), yielding a tripartite complex comprised of a stable signal peptide (SSP), the receptor-binding GP1, and the fusion-active ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.10.038

    authors： Burri DJ,Pasquato A,da Palma JR,Igonet S,Oldstone MB,Kunz S

    更新日期：2013-02-05 00:00:00

  • Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection.

    abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of natura...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.07.009

    authors： Goldberg TL,Lowe JF,Milburn SM,Firkins LD

    更新日期：2003-12-20 00:00:00

  • The Epstein-Barr virus latent membrane protein 2A PY motif recruits WW domain-containing ubiquitin-protein ligases.

    abstract：:Latent membrane protein 2A (LMP2A) is expressed in latent Epstein-Barr virus (EBV) infection. LMP2A functions to downregulate B-cell signal transduction and viral reactivation from latency in EBV-immortalized B cells in vitro, and acts to provide B cells with both a survival and developmental signal in vivo. Identific...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0166

    authors： Ikeda M,Ikeda A,Longan LC,Longnecker R

    更新日期：2000-03-01 00:00:00

  • Reduced antigenicity of the hepatitis B virus HBsAg protein arising as a consequence of sequence changes in the overlapping polymerase gene that are selected by lamivudine therapy.

    abstract：:The prevalence of hepatitis B virus vaccine escape mutants has increased as a consequence of the introduction of global vaccination programs. Furthermore and as a consequence of the organization of the genome of hepatitis B virus (HBV) into overlapping reading frames, the selection of polymerase mutants during long-te...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1246

    authors： Torresi J,Earnest-Silveira L,Deliyannis G,Edgtton K,Zhuang H,Locarnini SA,Fyfe J,Sozzi T,Jackson DC

    更新日期：2002-02-15 00:00:00

  • The complete genome structure and synthesis of infectious RNA from clones of tomato bushy stunt virus.

    abstract：:The complete sequence of the genome of the cherry strain of tomato bushy stunt virus (TBSV), a member of the tombusvirus group, was determined. A full-length clone of the genome containing a bacteriophage T7 RNA polymerase promoter was assembled from partial cDNA clones. In vitro transcripts of the genome, either with...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90468-7

    authors： Hearne PQ,Knorr DA,Hillman BI,Morris TJ

    更新日期：1990-07-01 00:00:00

  • Stable cell lines expressing high levels of the herpes simplex virus type 1 LAT are refractory to caspase 3 activation and DNA laddering following cold shock induced apoptosis.

    abstract：:The herpes simplex virus type 1 (HSV-1) latency associated transcript (LAT) gene's anti-apoptosis activity plays a central, but not fully elucidated, role in enhancing the virus's reactivation phenotype. In transient transfection experiments, LAT increases cell survival following an apoptotic insult in the absence of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.07.023

    authors： Carpenter D,Hsiang C,Brown DJ,Jin L,Osorio N,BenMohamed L,Jones C,Wechsler SL

    更新日期：2007-12-05 00:00:00

  • Mutated forms of human cytomegalovirus glycoprotein B are impaired in inducing syncytium formation.

    abstract：:Human cytomegalovirus (HCMV) glycoprotein B (gB) promotes virion entry into cells by fusing the virion envelope with the cellular membrane. We recently reported that UB cells (U373 glioblastoma cells constructed to produce HCMV gB constitutively) form multinucleate syncytia that are dependent on the density of gB in t...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1290

    authors： Tugizov S,Wang Y,Qadri I,Navarro D,Maidji E,Pereira L

    更新日期：1995-06-01 00:00:00

  • Gut microbiota is associated with protection against Marek's disease virus infection in chickens.

    abstract：:Marek's Disease Virus (MDV) infects chickens via respiratory route and causes lymphomas in internal organs including gastrointestinal tract. MDV infection causes a shift in the gut microbiota composition. However, interactions between the gut microbiota and immune responses against MDV infection are not well understoo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.10.011

    authors： Bavananthasivam J,Astill J,Matsuyama-Kato A,Taha-Abdelaziz K,Shojadoost B,Sharif S

    更新日期：2021-01-15 00:00:00

  • Interferon-beta and interferon-gamma synergistically inhibit the replication of severe acute respiratory syndrome-associated coronavirus (SARS-CoV).

    abstract：:Recent studies have shown that interferon-gamma (IFN-gamma) synergizes with IFN-alpha/beta to inhibit the replication of both RNA and DNA viruses. We investigated the effects of IFNs on the replication of two strains of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). While treatment of Vero E6 cel...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.08.011

    authors： Sainz B Jr,Mossel EC,Peters CJ,Garry RF

    更新日期：2004-11-10 00:00:00

  • The retroviral cyclin of walleye dermal sarcoma virus binds cyclin-dependent kinases 3 and 8.

    abstract：:Walleye dermal sarcoma virus encodes a retroviral cyclin (rv-cyclin) with a cyclin box fold and transcription activation domain (AD). Co-immune precipitation (co-IP) identified an association of rv-cyclin with cyclin-dependent kinase 8 (cdk8). Cdk8 is dependent upon cyclin C and regulates transcription with the Mediat...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.10.022

    authors： Brewster CD,Birkenheuer CH,Vogt MB,Quackenbush SL,Rovnak J

    更新日期：2011-01-20 00:00:00

  • The host Pex19p plays a role in peroxisomal localization of tombusvirus replication proteins.

    abstract：:Replication of Tomato bushy stunt virus (TBSV) RNA takes place on the cytosolic membrane surface of peroxisomes in plants and in yeast, a model host. To identify the host proteins involved in assisting the peroxisomal localization of the tombusvirus p33 replication protein, we tested if p33 could bind directly to yeas...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.06.044

    authors： Pathak KB,Sasvari Z,Nagy PD

    更新日期：2008-09-30 00:00:00

  • In vitro differentiation of monocytoid THP-1 cells affects their permissiveness for HIV strains: a model system for studying the cellular basis of HIV differential tropism.

    abstract：:The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Vir...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1121

    authors： Meylan PR,Spina CA,Richman DD,Kornbluth RS

    更新日期：1993-03-01 00:00:00

  • Mu-opioid modulation of HIV-1 coreceptor expression and HIV-1 replication.

    abstract：:A substantial proportion of HIV-1-infected individuals are intravenous drug users (i.v.DUs) who abuse opiates. Opioids induce a number of immunomodulatory effects that may directly influence HIV-1 disease progression. In the present report, we have investigated the effect of opioids on the expression of the major HIV-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00015-1

    authors： Steele AD,Henderson EE,Rogers TJ

    更新日期：2003-04-25 00:00:00

  • BST-2 mediated restriction of simian-human immunodeficiency virus.

    abstract：:Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the trans...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.07.021

    authors： Ruiz A,Lau D,Mitchell RS,Hill MS,Schmitt K,Guatelli JC,Stephens EB

    更新日期：2010-10-25 00:00:00

  • The genome-linked protein of cowpea mosaic virus is coded by RNA from the bottom component.

    abstract：:It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(80)90239-1

    authors： Stanley J,Goldbach R,Van Kammen A

    更新日期：1980-10-15 00:00:00