A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of Rev.

abstract：:Specific binding sites for Epstein-Barr virus (EBV) nuclear antigen (EBNA-1), isolated and semipurified from EBV-transformed nonproductive Raji cells, were visualized on the molecule of EBV DNA by electron microscopy and mapped. Two measures had to be applied to counteract the limited purity of the EBNA-1 preparation:...

journal_title：Virology

authors： Reischig J,Bartsch D,Polack A,Vonka V,Hirsch I

更新日期：1987-10-01 00:00:00

abstract：:A central, yet unresolved issue in the pathogenesis of HIV disease is the mechanism of antibody perturbation. In this study, HIV-specific memory B-cells were quantified in groups of infected subjects and compared with memory responses to other antigens and antibody titers. HIV-specific memory B-cell responses were vig...

journal_title：Virology

authors： Bussmann BM,Reiche S,Bieniek B,Krznaric I,Ackermann F,Jassoy C

更新日期：2010-02-05 00:00:00

abstract：:In 1943 Burnet reported on changes in the hemagglutinating properties of human influenza virus which occurred during adaptation of the virus to growth in chicken eggs. Only recently has direct evidence been presented that these changes affect the antigenic properties of the virus. Schild et al. (G. C. Schild, J. S. Ox...

journal_title：Virology

authors： Robertson JS,Naeve CW,Webster RG,Bootman JS,Newman R,Schild GC

更新日期：1985-05-01 00:00:00

abstract：:We have determined the nucleotide sequence of the RNA2 segment of the Nudaurelia capensis omega virus genome. It was found to consist of 2448 nucleotides and contained one long open reading frame (ORF) encoding the 644 residue capsid protein. The deduced amino acid sequence of this protein reveals a positively charged...

journal_title：Virology

authors： Agrawal DK,Johnson JE

更新日期：1992-10-01 00:00:00

abstract：:HCV genotype is a major determinant of clinical outcome, and GT1b HCV infection is the most difficult to treat and also the predominant genotype in East Asia and Europe. We developed 1b/JFH-1 inter-genotypic recombinants containing the structural genes (Core, E1, E2), p7 and the 1stTMD of NS2 directly from GT1b clinic...

journal_title：Virology

authors： Lu J,Tao W,Li R,Xiang Y,Zhang N,Xiang X,Xie Q,Zhong J

更新日期：2013-08-15 00:00:00

abstract：:The phosphorylated protein pp65 of human Cytomegalovirus (HCMV) is the predominant virion protein and the major tegument constituent. It plays important roles in HCMV infection and virion assembly. Live cell imaging and fluorescence recovery after photobleaching (FRAP) analysis showed that HCMV pp65 accumulated dynami...

journal_title：Virology

authors： Cui Z,Zhang K,Zhang Z,Liu Y,Zhou Y,Wei H,Zhang XE

更新日期：2009-09-30 00:00:00

abstract：:Although dengue (DEN) virus is the etiologic agent of dengue fever, the most prevalent vector-borne viral disease in the world, precise information on the antigenic structure of the dengue virion is limited. We have prepared a set of murine monoclonal antibodies (MAbs) specific for the envelope (E) glycoprotein of DEN...

journal_title：Virology

authors： Roehrig JT,Bolin RA,Kelly RG

更新日期：1998-07-05 00:00:00

abstract：:The effects of P2 prophage on lambda-mediated depression of host metabolism, and on lambda macromolecular synthesis have been studied. Experiments employing certain lambdabio deletion mutants and lambda-related phages suggest that the same gene products are involved in inhibition of host metabolism (Hin+ function) and...

journal_title：Virology

authors： Cohen SN,Chang AC

更新日期：1971-11-01 00:00:00

abstract：:An effective type I interferon (IFN-alpha/beta) response is critical for the control of many viral infections. Using an oncolytic strain of vesicular stomatitis virus, we have examined the cross-talk between virus-induced apoptosis and initiation of innate immune response. The intrinsic apoptotic cascade, specifically...

journal_title：Virology

authors： Sharif-Askari E,Nakhaei P,Oliere S,Tumilasci V,Hernandez E,Wilkinson P,Lin R,Bell J,Hiscott J

更新日期：2007-08-15 00:00:00

abstract：:The 121,752-bp genome sequence of bacteriophage T5 was determined; the linear, double-stranded DNA is nicked in one of the strands and has large direct terminal repeats of 10,139 bp (8.3%) at both ends. The genome structure is consistently arranged according to its lytic life cycle. Of the 168 potential open reading f...

journal_title：Virology

authors： Wang J,Jiang Y,Vincent M,Sun Y,Yu H,Wang J,Bao Q,Kong H,Hu S

更新日期：2005-02-05 00:00:00

abstract：:Using the 5' long terminal repeat (LTR) as its only promoter, the HTLV-1 provirus generates a single RNA transcript that undergoes differential splicing to express the various viral proteins. Examination of sequence near the transcription start site revealed an element resembling a transcriptional initiator (Inr) at p...

journal_title：Virology

authors： Wycuff DR,Goff MD,Marriott SJ

更新日期：2001-02-01 00:00:00

abstract：:In order to establish whether the poor growth of the two fastidious adenoviruses types 40 and 41 (Ad40 and Ad41) in HeLa cells is due to a reduced trans-activation by the early region 1A (E1A), we have determined the trans-activating effect of this region on the expression of the chloramphenicol acetyltransferase (CAT...

journal_title：Virology

authors： van Loon AE,Gilardi P,Perricaudet M,Rozijn TH,Sussenbach JS

更新日期：1987-09-01 00:00:00

abstract：:The Sindbis virus mutant SB-RL, in contrast to its parent, Sindbis strain AR339 (SB), is attenuated in neonatal mice, has an increased rate of penetration in tissue culture cells, and is more sensitive to neutralization by E2-specific monoclonal antibodies (MCAbs) R6 and R13. These phenotypic differences are controlle...

journal_title：Virology

authors： Pence DF,Davis NL,Johnston RE

更新日期：1990-03-01 00:00:00

abstract：:The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography em...

journal_title：Virology

authors： Goldschmidts W,Luka J,Pearson GR

更新日期：1987-03-01 00:00:00

abstract：:Transcription of human papillomaviruses (HPV) is regulated by enhancer sequences located in the upstream regulatory region. The factors regulating expression of one of the high risk genital HPV types, HPV 31b, were investigated using transient expression and protein binding assays. A region of 262 base pairs in length...

journal_title：Virology

authors： Kyo S,Tam A,Laimins LA

更新日期：1995-08-01 00:00:00

abstract：:The target for neutralizing antibodies against human immunodeficiency virus (HIV) is the trimeric Env protein on the native virion. Conserved neutralizing epitopes of receptor binding sites are located in the recessed core of the Env protein, partially masked by glycosylations and variable loops. In this study, we hav...

journal_title：Virology

authors： Kang SM,Quan FS,Huang C,Guo L,Ye L,Yang C,Compans RW

更新日期：2005-01-05 00:00:00

abstract：:The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...

journal_title：Virology

authors： Porter DC,Ansardi DC,Wang J,McPherson S,Moldoveanu Z,Morrow CD

更新日期：1998-03-30 00:00:00

abstract：:Using a defective interfering Sendai virus stock (DIH4) freed of nondefective helper virus, we found that the closely related parainfluenza viruses 1 and 3 could substitute for the Sendai virus helper in replicating DIH4, creating chimeric nucleocapsids. The morbillivirus measles and the rhabdovirus VSV could not subs...

journal_title：Virology

authors： Curran JA,Kolakofsky D

更新日期：1991-05-01 00:00:00

abstract：:In transient gene expression assays we observed an increase in expression of the bacterial chloramphenicol acetyl-transferase (CAT) gene, under the transcriptional control of the HIV-1 LTR (pLTR-CAT), when this plasmid was cotransfected into Vero or MRC-5 cells with a plasmid containing either the HCMV immediate early...

journal_title：Virology

authors： Rando RF,Srinivasan A,Feingold J,Gonczol E,Plotkin S

更新日期：1990-05-01 00:00:00

abstract：:Herpes simplex virus 1 glycoprotein B (gB) is one of 10 glycoproteins in the virion envelope and in the membranes of infected cells. It is required for infection of cells in culture and functions in penetration of the cell by fusing the virion envelope with the plasma membrane. In studies to map the functional domains...

journal_title：Virology

authors： Navarro D,Paz P,Pereira L

更新日期：1992-01-01 00:00:00

abstract：:We have previously adapted an experimental system which supports autonomous replication of human papillomavirus-11 (HPV-11) genomic DNA in a human squamous carcinoma cell line (SCC-4) following electroporation of linearized viral DNA. This system allows evaluation of HPV-11 transcription in the context of replicating ...

journal_title：Virology

authors： Renaud KJ,Cowsert LM

更新日期：1996-06-01 00:00:00

abstract：:Prior genetic analysis suggests that there may exist an interaction between the products of the vaccinia virus genes A18R, a putative negative transcription elongation factor, and G2R, a putative positive transcription elongation factor. In addition, affinity purification of polyhistidine-tagged G2R protein overexpres...

journal_title：Virology

authors： Black EP,Moussatche N,Condit RC

更新日期：1998-06-05 00:00:00

abstract：:Natural antibodies to gp41 inhibit HIV-1 replication through the recognition of two different regions, corresponding to the leucine zipper motif in the HR1 alpha-helix and to another motif within HR2 region, hosting 2F5 and 4E10 epitope. This study aimed at reproducing such protective responses through VLP vaccination...

journal_title：Virology

authors： Pastori C,Tudor D,Diomede L,Drillet AS,Jegerlehner A,Röhn TA,Bomsel M,Lopalco L

更新日期：2012-09-15 00:00:00

abstract：:Cytoplasmic poly(A)-containing RNA from parainfluenza virus 3-infected cells was used as template to construct a cDNA library that was cloned into the EcoRV site of the plasmid pMG5. The resulting clones were screened with [32P]-labeled cDNA probes made from infected and mock-infected cell mRNAs. The virus specificity...

journal_title：Virology

authors： Sánchez A,Banerjee AK

更新日期：1985-11-01 00:00:00

abstract：:The antibody response in serum and the reproductive tract of female rabbits to a model antigen, influenza virus haemagglutinin (HA), encoded by a recombinant myxoma virus was investigated. Strong and lasting IgG antibody responses to HA were induced in serum following intradermal, intranasal, and intravaginal immunisa...

journal_title：Virology

authors： Gu W,Holland M,Janssens P,Kerr P

更新日期：2003-08-15 00:00:00

abstract：:Hepatitis C virus (HCV) nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp) has acquired a unique beta-hairpin in the thumb subdomain which protrudes toward the active site. We report here that this beta-hairpin plays an important role in positioning the 3' terminus of the viral RNA genome for correct ...

journal_title：Virology

authors： Hong Z,Cameron CE,Walker MP,Castro C,Yao N,Lau JY,Zhong W

更新日期：2001-06-20 00:00:00

abstract：:Tobacco mosaic virus (TMV) RNA was encapsulated in large unilamellar vesicles (LUV) of phosphatidylserine and was introduced into protoplasts from Vinca rosea suspension cultures. Infection could be effected by brief incubation of protoplasts with the LUV containing TMV-RNA in the presence of either polyethylene glyco...

journal_title：Virology

authors： Fukunaga Y,Nagata T,Takebe I

更新日期：1981-09-01 00:00:00

abstract：:The mature Gn glycoprotein of Crimean Congo hemorrhagic fever (CCHF) virus contains two predicted glycosylation sites (557N and 755N). Of these, N-glycans are added only at 557N, as evidenced by abrogation of Gn-glycosylation by mutation of 557N but not 755N site. Mutational block of Gn-glycosylation at 557N did not s...

journal_title：Virology

authors： Erickson BR,Deyde V,Sanchez AJ,Vincent MJ,Nichol ST

更新日期：2007-05-10 00:00:00

abstract：:The helix-destabilizing properties of a single-stranded DNA-binding protein, LEF-3, of Bombyx mori nucleopolyhedrovirus (BmNPV) were studied. Partial duplexes of DNA containing single-stranded (ss) tails of different sizes and orientations were used as substrates for assay of the unwinding ability of LEF-3. Upon nonco...

journal_title：Virology

authors： Mikhailov VS

更新日期：2000-04-25 00:00:00

abstract：:To investigate the role of Tobacco vein banding mosaic virus (TVBMV) 3'-UTR in virus systemic infection, three types of deletions were introduced into TVBMV infectious clone pCaTVBMV-GFP. Mutants with deletions at the nucleotide position 8-42, 43-141, or 163-174 in the 3'-UTR failed to cause systemic infection in N. b...

journal_title：Virology

authors： Wang Y,Yang ZY,Tian YP,Geng C,Yuan XF,Li XD

更新日期：2019-01-15 00:00:00