Abstract:
:Tobacco mosaic virus (TMV) RNA was encapsulated in large unilamellar vesicles (LUV) of phosphatidylserine and was introduced into protoplasts from Vinca rosea suspension cultures. Infection could be effected by brief incubation of protoplasts with the LUV containing TMV-RNA in the presence of either polyethylene glycol or polyvinyl alcohol. The presence of these polymers was essential for infection, and postinoculation washing with the high pH-high Ca2+ buffer enhanced infection significantly. Up to 80% of protoplasts could be infected under the optimal conditions as demonstrated by immunofluorescence technique. Calculations showed that around 2 x 10(6) TMV-RNA molecules were added per infected protoplast, indicating that the efficiency of infection by this method compares favorably with those by the existing methods for inoculating protoplasts from mesophyll cells with TMV-RNA. The significance of using protoplasts from cultured cells for infection with plant viruses and their nucleic acids is discussed.
journal_name
Virologyjournal_title
Virologyauthors
Fukunaga Y,Nagata T,Takebe Idoi
10.1016/0042-6822(81)90203-8subject
Has Abstractpub_date
1981-09-01 00:00:00pages
752-60issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(81)90203-8journal_volume
113pub_type
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