Abstract:
:Hepatitis C virus (HCV) core protein is a multifunctional protein interacting with cellular and viral proteins and promoters. A tetracycline-regulated system was used to generate a HepG2 Tet-Off cell line allowing regulated expression of a full-length (191 aa) and an N(c)-truncated core protein (160 aa). In this system HCV core protein expression activates extracellular signal-regulated kinase (ERK), c-jun N-terminal kinase (JNK), and p38 mitogen-activated protein (MAP) kinase, induces MAP kinase phosphatase MKP-1 expression, and increases cell proliferation. This was accompanied by an activation of c-Jun and ATF-2, but not Elk-1 and c-Fos. Furthermore, AP-1 activation was independent of c-Fos. Full-length and N(c)-truncated HCV core proteins exerted similar effects.
journal_name
Virologyjournal_title
Virologyauthors
Erhardt A,Hassan M,Heintges T,Häussinger Ddoi
10.1006/viro.2001.1227subject
Has Abstractpub_date
2002-01-20 00:00:00pages
272-84issue
2eissn
0042-6822issn
1096-0341pii
S0042682201912279journal_volume
292pub_type
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