Abstract:
:Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a highly contagious coronavirus of chicken. rcMBL inhibited in a dose-dependent manner the infection of BHK-21 cells by IBV-Beaudette, as detected by immunofluorescence staining of viral proteins and qPCR. ELISA and negative staining electron microscopy showed that rcMBL bound directly to IBV, resulting in the aggregation of viral particles. Furthermore, we demonstrated that MBL bound specifically to the spike S1 protein of IBV which mediates viral attachment. This subsequently blocked the attachment of S1 to IBV-susceptible cells in chicken tracheal tissues as shown in protein histochemistry. Taken together, rcMBL exhibits antiviral activity against IBV, based on a direct interaction with IBV virions.
journal_name
Virologyjournal_title
Virologyauthors
Zhang W,Bouwman KM,van Beurden SJ,Ordonez SR,van Eijk M,Haagsman HP,Verheije MH,Veldhuizen EJAdoi
10.1016/j.virol.2017.06.028subject
Has Abstractpub_date
2017-09-01 00:00:00pages
252-259eissn
0042-6822issn
1096-0341pii
S0042-6822(17)30205-2journal_volume
509pub_type
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