Abstract:
:Pokeweed antiviral protein (PAP) is a ribosome-inactivating protein characterized by its ability to depurinate the sarcin/ricin (S/R) loop of the large rRNA of prokaryotic and eukaryotic ribosomes. Here, a series of PAP mutants were used to examine the relationship between depurination of the S/R loop and inhibition of +1 programmed ribosomal frameshifting (PRF) and to define PAP sequences critical for inhibition of +1 PRF and Ty1 retrotransposition in the yeast Saccharomyces cerevisiae. Using three different classes of mutants we present evidence that strong binding of a C-terminal PAP mutant (PAPc) to ribosomes is sufficient to inhibit +1 PRF and Ty1 retrotransposition in the absence of S/R loop depurination. PAPc did not affect the totivirus ScV-L-A and HIV-1-directed -1 PRF efficiencies or the ability of cells to maintain the M(1)-dependent killer phenotype, demonstrating the specificity of the effect of PAPc on +1 PRF.
journal_name
Virologyjournal_title
Virologyauthors
Hudak KA,Hammell AB,Yasenchak J,Tumer NE,Dinman JDdoi
10.1006/viro.2000.0647subject
Has Abstractpub_date
2001-01-05 00:00:00pages
292-301issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(00)90647-0journal_volume
279pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Hepatitis C virus (HCV) encodes a polyprotein that is processed to produce the structural and nonstructural proteins of the virus. Nonstructural protein 3 (NS3) is a serine proteinase that cleaves the polyprotein to release the NS4A, NS4B, NS5A, and NS5B proteins. To characterize the substrate specificity of NS3, we s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1520
更新日期:1994-10-01 00:00:00
abstract::Human cytomegalovirus (HCMV) can be isolated from peripheral blood leukocytes; however, in vitro, only abortive infection of monocytes, lymphocytes, and granulocytes has been detected. These studies demonstrate that freshly isolated monocytes can be infected with HCMV. Infection of monocytes was not associated with lo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1383
更新日期:1993-08-01 00:00:00
abstract::Although v-src, the oncogene of Rous sarcoma virus, has been shown to be capable of inducing lethal tumors at visceral sites distal to the primary tumor mass, the mechanisms opposing visceral tumor formation remain to be elucidated. In the present study, we show that visceral tumors, many of which represent a metastas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1617
更新日期:1993-11-01 00:00:00
abstract::The sequence of the 5'-end of the genome of dengue 2 (Jamaica genotype) virus has been previously reported (V. Deubel, R. M. Kinney, and D. W. Trent, 1986, Virology 155, 365-377). We have now cloned and sequenced the remaining 75% of the genomic RNA that encodes the nonstructural proteins. The complete genome is 10,72...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90677-0
更新日期:1988-07-01 00:00:00
abstract::The open reading frame EP153R, located within the EcoRI E' fragment of the African swine fever (ASF) virus genome, is predicted to encode a membrane protein of 153 amino acids that presents significant homology to the N-terminal region of several CD44 molecules. EP153R contains multiple putative sites for N-glycosylat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0080
更新日期:2000-01-20 00:00:00
abstract::Transcription of human papillomaviruses (HPV) is regulated by enhancer sequences located in the upstream regulatory region. The factors regulating expression of one of the high risk genital HPV types, HPV 31b, were investigated using transient expression and protein binding assays. A region of 262 base pairs in length...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1390
更新日期:1995-08-01 00:00:00
abstract::For purposes of gene therapy, the tropism of adenovirus (Ad) serotype 5 vectors can be altered with fibers derived from alternative serotypes. However, there is currently limited information available on the cellular receptors used by the approximately 51 known Ad serotypes. Recently, alpha(2-->3)-linked sialic acid (...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.04.001
更新日期:2004-06-20 00:00:00
abstract::Measles virus transcription generates multiple P/C gene-specific mRNAs by a process which has been termed editing. In one of these mRNAs, the cotranscriptional addition of a single nontemplated G residue allows translational access to the V protein reading frame. The protein translated from this mRNA has been called V...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1050
更新日期:1994-01-01 00:00:00
abstract::Virus like particles (17 nm in diameter) associated with maize white line mosaic virus (MWLMV) were shown to be a satellite virus of MWLMV (SV-MWLMV) on the basis of the following properties: (1) The SV-MWLMV was dependent upon the presence of MWLMV for replication in maize, while the latter virus could replicate inde...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90060-o
更新日期:1991-02-01 00:00:00
abstract::Oral-gastric delivery of vaccines is a preferred route of immunization and is particularly relevant to the development of vaccine-vector systems. We have investigated the ability of a replication deficient (E1-deleted) adenovirus construct (RAd68), which efficiently expresses the measles virus nucleocapsid (N) protein...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1281
更新日期:2002-02-15 00:00:00
abstract::CD8+ T lymphocytes are critical for the control of gammaherpesvirus latency. To determine how memory CD8+ T cells generated during latency differ from those primed during acute or chronic viral infection, we adoptively transferred naive P14 CD8+ T cells into uninfected recipients, and examined surface proteins, cytoki...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.06.018
更新日期:2017-09-01 00:00:00
abstract::Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00348-9
更新日期:2003-09-15 00:00:00
abstract::About 100 nucleotides of DNA sequence at the 5' noncoding region of the Choristoneura biennis entomopoxvirus spheroidin gene was chemically synthesized and inserted into a vaccinia expression vector, interrupting the vaccinia thymidine kinase gene. When the bacterial beta-galactosidase gene was introduced downstream o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90070-r
更新日期:1991-02-01 00:00:00
abstract::Within the family of Poxviridae, the entomopoxviruses are the most distant relatives of the more well-known and intensively studied orthopoxviruses (vaccinia and variola). The growth of the entomopoxvirus from Amsacta moorei (AmEPV) has been characterized in cell culture and compared to that of vaccinia virus (VV), th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1428
更新日期:1995-08-20 00:00:00
abstract::We investigated whether culture conditions could affect the RANTES antiviral effect on human immunodeficiency virus type 1 (HIV-1) infection of primary macrophages. Monocyte-derived macrophages (MDM) were obtained either as (1) the adherent cells of 5-day cultures of blood mononuclear cells (PBMC), followed by 2 days ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9452
更新日期:1998-12-05 00:00:00
abstract::Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phag...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90130-2
更新日期:1984-05-01 00:00:00
abstract::Expression of the SV40 large T-antigen allows primary cells to escape senescence and thereby become immortalized. Immortalization occurs in two steps, extension of life span and acquisition of unlimited cell division potential. By following the increase in expression of a senescence-associated marker with increased ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9056
更新日期:1998-04-10 00:00:00
abstract::Middle East respiratory syndrome coronavirus (MERS-CoV) infects host cells through binding the receptor binding domain (RBD) on its spike glycoprotein to human receptor dipeptidyl peptidase 4 (hDPP4). Here, we report identification of critical residues on hDPP4 for RBD binding and virus entry through analysis of a pan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.10.006
更新日期:2014-12-01 00:00:00
abstract::In order to establish whether the poor growth of the two fastidious adenoviruses types 40 and 41 (Ad40 and Ad41) in HeLa cells is due to a reduced trans-activation by the early region 1A (E1A), we have determined the trans-activating effect of this region on the expression of the chloramphenicol acetyltransferase (CAT...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90080-8
更新日期:1987-09-01 00:00:00
abstract::Matrix protein M1 purified from influenza A and B viruses has been analyzed for its ability to specifically interact with cellular proteins by immune coprecipitation and by an in vitro binding assay on nitrocellulose on PVDF membranes. When M1 was mixed with lysates of uninfected cells there was selective binding of h...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8700
更新日期:1997-09-01 00:00:00
abstract::MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90043-6
更新日期:1984-01-30 00:00:00
abstract::Mixed infection of nonpermissive bacteria by amber mutant and wild-type T4 phage reduces the burst size in a manner that depends on the nature of the gene product eliminated by the mutation. While the genotypic proportions produced by such mixedly infected cells were directly proportional to those in the starting mixt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90011-4
更新日期:1984-08-01 00:00:00
abstract::The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90140-0
更新日期:1985-12-01 00:00:00
abstract::The formation of a lipoprotein membrane within specialized areas of the cytoplasm is the first visible step in poxvirus morphogenesis. The A17L viral protein, an essential nonglycosylated membrane component, was predicted to have four centrally located alpha-helical membrane-spanning domains. The gene was expressed as...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9870
更新日期:1999-09-01 00:00:00
abstract::Previous studies have shown that a 558-bp fragment of human cytomegalovirus (CMV) DNA contained within pCM4127 and designated CMV mtr can morphologically transform rodents cells in vitro. By cotransformation with pCM4127 and a plasmid conferring G418 resistance, pSV2neo, morphologically transformed NIH3T3 cell lines w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90437-5
更新日期:1987-01-01 00:00:00
abstract::The v-erb B protein of avian erythroblastosis virus (AEV) possesses an associated protein kinase activity in vitro. Analysis of temperature-sensitive mutants, and nonconditional host range mutants of AEV demonstrated that there was no simple correlation between this autophosphorylation activity and the transformation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90287-4
更新日期:1986-04-15 00:00:00
abstract::Human immunodeficiency virus type 1 subtype C (HIV-1C) accounts for about 50% of all HIV infections in the pandemic and is the predominant subtype in the heavily burdened region of southern Africa. HIV-1C possesses unique genetic and phenotypic features that might be associated with biological differences compared to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.047
更新日期:2006-04-10 00:00:00
abstract::NF-kappaB comprises a family of transcription factors that regulate key immune processes. In this study, the effects of orthopoxvirus infection upon the activation of NF-kappaB were examined. During the early phase of infection, cowpox virus can inhibit the induction of NF-kappaB-regulated gene expression by interferi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1090
更新日期:2001-09-15 00:00:00
abstract::HPV-16 is the major causes of cervical cancer. Persistence of infection is a necessary event for progression of the infection to cancer. Among other factors, virus persistence is due the viral proteins fighting the immune response. HPV-16 E5 down-regulates MHC/HLA class I, which is much reduced on the cell surface and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.07.044
更新日期:2010-11-10 00:00:00
abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.08.032
更新日期:2016-11-01 00:00:00