The effect of inhibitors on the growth of the entomopoxvirus from Amsacta moorei in Lymantria dispar (gypsy moth) cells.


:Within the family of Poxviridae, the entomopoxviruses are the most distant relatives of the more well-known and intensively studied orthopoxviruses (vaccinia and variola). The growth of the entomopoxvirus from Amsacta moorei (AmEPV) has been characterized in cell culture and compared to that of vaccinia virus (VV), the prototypic orthopoxvirus. The overall characteristics of infected cell cultures were generally similar between the two viruses. One striking difference noted was the apparent absence of proteolytic processing of late AmEPV viral proteins, a hallmark of vertebrate poxvirus infections associated with viral morphogenesis. AmEPV, like VV, was found to be sensitive to all the inhibitors of viral infection tested including phosphonoacetic acid, 1-beta-D-arabinofuranosylcytosine, and isatin-beta-thiosemicarbazone (IBT), a compound associated with the rather specific inhibition of vertebrate poxviruses. While both VV and AmEPV are inhibited by IBT, the inhibition of AmEPV, unlike that of VV, is not accompanied by either a breakdown of ribosomal RNA or a global inhibition of late viral protein synthesis. Instead, in the presence of IBT, AmEPV enveloped, immature virions form devoid of a well-differentiated core, which unlike mature virions fail to insert into occlusion bodies.






Winter J,Hall RL,Moyer RW




Has Abstract


1995-08-20 00:00:00
















  • Demonstration of the specificity of poliovirus encapsidation using a novel replicon which encodes enzymatically active firefly luciferase.

    abstract::The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...


    pub_type: 杂志文章


    authors: Porter DC,Ansardi DC,Wang J,McPherson S,Moldoveanu Z,Morrow CD

    更新日期:1998-03-30 00:00:00

  • Human papillomavirus type 16 E7 protein expressed in Escherichia coli and monkey COS-1 cells: immunofluorescence detection of the nuclear E7 protein.

    abstract::Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...


    pub_type: 杂志文章


    authors: Sato H,Watanabe S,Furuno A,Yoshiike K

    更新日期:1989-05-01 00:00:00

  • Human immunodeficiency virus (HIV-1) cytotoxicity: perturbation of the cell membrane and depression of phospholipid synthesis.

    abstract::The molecular mechanism(s) by which human immunodeficiency virus (HIV-1) injures a T-cell line was studied. A pathological role for viral env proteins, which are inserted into the plasma membrane, has been previously demonstrated for HIV as well as other retroviruses which are cytopathic. We therefore initiated studie...


    pub_type: 杂志文章


    authors: Lynn WS,Tweedale A,Cloyd MW

    更新日期:1988-03-01 00:00:00

  • Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion's surface.

    abstract::The sequence of the 1491 nucleotides found at the 3' end of the genome of the highly aphid-transmissible (HAT) isolate of tobacco etch virus (TEV) has been determined. The nucleotide sequence of the capsid protein gene has been identified and compared with the corresponding region of the not-aphid-transmissible (NAT) ...


    pub_type: 杂志文章


    authors: Allison RF,Dougherty WG,Parks TD,Willis L,Johnston RE,Kelly M,Armstrong FB

    更新日期:1985-12-01 00:00:00

  • A sequence in HpaI-P fragment of herpes simplex virus-1 DNA determines intraperitoneal virulence in mice.

    abstract::The virulence of herpes simplex virus-1 (HSV-1) strains by the intraperitoneal (ip) route of injection in mice depends on the presence of an intact sequence in the HpaI DNA fragment P within coordinates 0.762 to 0.787. Deletion of the HpaI-P region (e.g., strain HFEM) abrogates the ability of the virus to infect mice ...


    pub_type: 杂志文章


    authors: Becker Y,Hadar J,Tabor E,Ben-Hur T,Raibstein I,Rösen A,Darai G

    更新日期:1986-03-01 00:00:00

  • Determination of a splice acceptor site of pX gene in HTLV-I infected cells.

    abstract::The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine e...


    pub_type: 杂志文章


    authors: Okamoto T,Josephs SF,Kawanishi M,Wong-Staal F

    更新日期:1985-06-01 00:00:00

  • Characterization of a novel alphaherpesvirus associated with fatal infections of domestic rabbits.

    abstract::A virus was found to be associated with a severe disease affecting rabbits on a farm near Anchorage, Alaska. Extracts from the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin, rabbit kidney, and Vero cells. Examination of the infectious agent by electron microscopy revealed an icosahe...


    pub_type: 杂志文章


    authors: Jin L,Löhr CV,Vanarsdall AL,Baker RJ,Moerdyk-Schauwecker M,Levine C,Gerlach RF,Cohen SA,Alvarado DE,Rohrmann GF

    更新日期:2008-08-15 00:00:00

  • Correlation of the genetic and physical maps of phage T1.

    abstract::A series of BglII- and Sau3A-derived fragments of phage T1 DNA were cloned into the plasmid vector pLV59 and the clones were tested for their ability to complement and recombine with infecting genomes of am mutants representing all known T1 essential genes. The resulting data were used to correlate the T1 genetic and ...


    pub_type: 杂志文章


    authors: Liebeschuetz J,Ritchie DA

    更新日期:1985-05-01 00:00:00

  • Interferon-λ4 is a cell-autonomous type III interferon associated with pre-treatment hepatitis C virus burden.

    abstract::Genetic variants surrounding the interferon-λ3 (IFNL3) gene are strongly associated with clearance of hepatitis C virus (HCV). A variant (rs368234815 TT/ΔG) upstream of IFNL3 was recently implicated to control expression of a novel gene termed IFNL4. We conducted genetic analysis of rs368234815 in a chronic HCV patien...


    pub_type: 临床试验,杂志文章


    authors: Lu YF,Goldstein DB,Urban TJ,Bradrick SS

    更新日期:2015-02-01 00:00:00

  • An EBNA-negative, EBV-genome-positive human lymphoblast cell line in which superinfecting EBV DNA is not maintained.

    abstract::A human B-lymphoid cell line, designated TG8, which does not express detectable levels of the EBV (Epstein-Barr virus)-associated nuclear antigen (EBNA), yet carries an average of one to two plasmid copies of the P3HR-1 EBV genome has been identified. TG8 can be superinfected by B95-8 EBV, resulting in up to 60-70% of...


    pub_type: 杂志文章


    authors: Reisman D,Sugden B

    更新日期:1984-08-01 00:00:00

  • Mouse xenotropic viruses can inhibit cell transformation.

    abstract::Mammalian type C retroviruses can be detected by their induction of foci of cell transformation in S+I - cells. We have noted that certain subtypes of the mouse xenotropic type C retrovirus (MuLV) inhibit this cell alteration. This inhibition, associated with intact virions, is irreversible and gives the infected cell...


    pub_type: 杂志文章


    authors: Hoffman AD,Levy JA

    更新日期:1987-09-01 00:00:00

  • Characterization of a MH2 mutant lacking the v-myc oncogene.

    abstract::We have previously reported that a virus, MH2-PA200, lacking the ability to transform quail embryo cells, could be isolated from wild type (wt) MH2 stocks passaged on chicken neuroretina cells. We report here the molecular cloning and extensive characterization of this MH2-PA200 provirus. Molecularly cloned MH2-PA200 ...


    pub_type: 杂志文章


    authors: Martin P,Henry C,Denhez F,Amouyel P,Bechade C,Calothy G,Debuire B,Stehelin D,Saule S

    更新日期:1986-09-01 00:00:00

  • Molecular characterization of Drosophila cells persistently infected with Flock House virus.

    abstract::Little is known about the molecular determinants causing and sustaining viral persistent infections at the cellular level. We found that Drosophila cells persistently infected (PI) with Flock House virus (FHV) invariably harbor defective viral RNAs, which are replicated by the FHV RNA-dependent RNA polymerase. Some de...


    pub_type: 杂志文章


    authors: Jovel J,Schneemann A

    更新日期:2011-10-10 00:00:00

  • Hepatitis C virus RNA polymerase and NS5A complex with a SNARE-like protein.

    abstract::Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discov...


    pub_type: 杂志文章


    authors: Tu H,Gao L,Shi ST,Taylor DR,Yang T,Mircheff AK,Wen Y,Gorbalenya AE,Hwang SB,Lai MM

    更新日期:1999-10-10 00:00:00

  • Inhibition of Rb and p53 is insufficient for SV40 T-antigen transformation.

    abstract::The SV40 large T-antigen (TAg) has proven useful in studying pathways involved with cell division and tissue homeostasis. TAg disrupts the normal action of tumor suppressors pRb and p53. It is unclear whether T-antigen inhibition of p53 and pRb is sufficient for oncogenic transformation or if additional T-antigen acti...


    pub_type: 杂志文章


    authors: Sachsenmeier KF,Pipas JM

    更新日期:2001-04-25 00:00:00

  • Epitopes of the G1 glycoprotein of La Crosse virus form overlapping clusters within a single antigenic site.

    abstract::Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...


    pub_type: 杂志文章


    authors: Najjar JA,Gentsch JR,Nathanson N,Gonzalez-Scarano F

    更新日期:1985-07-30 00:00:00

  • Different HIV pox viral vector-based vaccines and adjuvants can induce unique antigen presenting cells that modulate CD8 T cell avidity.

    abstract::The lung-derived dendritic cell (LDC) recruitment following intranasal (i.n.) vaccination of different poxviral vector-based vaccines/adjuvants were evaluated to decipher how these factors influenced CD8(+) T cell avidity. Compared to the standard i.n. recombinant fowlpox virus (FPV)-HIV vaccination, the FPV-HIV IL-13...


    pub_type: 杂志文章


    authors: Trivedi S,Jackson RJ,Ranasinghe C

    更新日期:2014-11-01 00:00:00

  • Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission.

    abstract::A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we dem...


    pub_type: 杂志文章


    authors: Wu L,Martin TD,Carrington M,KewalRamani VN

    更新日期:2004-01-05 00:00:00

  • Subcellular distribution of the foot-and-mouth disease virus 3A protein in cells infected with viruses encoding wild-type and bovine-attenuated forms of 3A.

    abstract::Picornavirus infection induces the proliferation and rearrangement of intracellular membranes in response to the synthesis of nonstructural proteins, including 3A. We have previously shown that changes in 3A are associated with the inability of a Taiwanese strain of foot-and-mouth disease virus (FMDV) (OTai) to grow i...


    pub_type: 杂志文章


    authors: O'Donnell VK,Pacheco JM,Henry TM,Mason PW

    更新日期:2001-08-15 00:00:00

  • Functional dissection of the ie2 gene product of the baculovirus Autographa californica nuclear polyhedrosis virus.

    abstract::The PstI-N genomic fragment of Autographa californica nuclear polyhedrosis virus (AcNPV) encodes an immediate-early protein, IE2, that functions as a promiscuous transactivator of other early viral promoters and heterologous promoters both in vivo and in vitro. IE2 contains several sequence motifs that are common to t...


    pub_type: 杂志文章


    authors: Yoo S,Guarino LA

    更新日期:1994-07-01 00:00:00

  • Primary structure of brome mosaic virus coat protein.

    abstract::The amino acid sequence of BMV (brome mosaic virus) coat protein is presented and compared with the sequence derived from RNA structure. Apart from removal of the N-terminal methionine residue and acetylation of the following serine residue, no post-translational modifications occur. ...


    pub_type: 杂志文章


    authors: Moosic JP,McKean DJ,Shih DS,Kaesberg P

    更新日期:1983-09-01 00:00:00

  • Adeno-associated virus infection of murine fibroblasts with help provided by mouse adenovirus.

    abstract::Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant p...


    pub_type: 杂志文章


    authors: Bhrigu V,Trempe JP

    更新日期:2009-07-20 00:00:00

  • The bovine papilloma virus E1 protein has ATPase activity essential to viral DNA replication and efficient transformation in cells.

    abstract::The bovine papilloma virus (BPV) E1 protein essential to viral DNA replication has recently been shown to associate via direct protein-DNA interactions with the viral origin of replication and to be an ATP-dependent helicase. We show here that in accordance with the latter function, the E1 gene product has intrinsic A...


    pub_type: 杂志文章


    authors: MacPherson P,Thorner L,Parker LM,Botchan M

    更新日期:1994-10-01 00:00:00

  • A subtype of human papillomavirus 5 (HPV-5b) and its subgenomic segment amplified in a carcinoma: nucleotide sequences and genomic organizations.

    abstract::A subtype of human papillomavirus 5 (HPV-5b) is closely associated with carcinomas in the disease epidermodysplasia verruciformis (EV). The complete genome was cloned from virus particles in benign lesions of a patient with EV and sequenced: it was 7779 nucleotides long and consisted of six open reading frames (ORFs) ...


    pub_type: 杂志文章


    authors: Yabe Y,Sakai A,Hitsumoto T,Kato H,Ogura H

    更新日期:1991-08-01 00:00:00

  • Mutagenesis occurring following infection with herpes simplex virus does not require virus replication.

    abstract::Infection of eukaryotic cells in culture with herpes simplex virus type-1 (HSV-1) or HSV-2 increased the mutation frequency of the supF gene carried on the shuttle vector pZ189 by around sixfold. The increase was apparent 2 hr postinfection and reached a peak after 8 hr. To investigate this mutagenesis, plasmids pCKRR...


    pub_type: 杂志文章


    authors: Clarke P,Clements JB

    更新日期:1991-06-01 00:00:00

  • Differential virus restriction patterns of rhesus macaque and human APOBEC3A: implications for lentivirus evolution.

    abstract::The human apolipoprotein B mRNA editing enzyme catalytic peptide-like 3 (APOBEC3; A3) family of proteins (A3A-H) are known to restrict various retroviruses and retroelements, but the full complement of rhesus macaque A3 proteins remains unclear. We report the isolation and characterization of the hA3A homologue from r...


    pub_type: 杂志文章


    authors: Schmitt K,Guo K,Algaier M,Ruiz A,Cheng F,Qiu J,Wissing S,Santiago ML,Stephens EB

    更新日期:2011-10-10 00:00:00

  • Failure of neutralizing antibody to regulate CAE lentivirus expression in vivo.

    abstract::Sequential isolates of caprine arthritis-encephalitis lentivirus (CAEV) were obtained from clinically arthritic carpal joints of three Saanen goats between 36.4 and 44.9 months after infection with biologically cloned CAEV isolate 63. These virus isolates and the CAEV-63 inoculum were evaluated for neutralization by a...


    pub_type: 杂志文章


    authors: Cheevers WP,McGuire TC,Norton LK,Cordery-Cotter R,Knowles DP

    更新日期:1993-10-01 00:00:00

  • Bombyx mori nucleopolyhedrovirus BM5 protein regulates progeny virus production and viral gene expression.

    abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mo...


    pub_type: 杂志文章


    authors: Kokusho R,Koh Y,Fujimoto M,Shimada T,Katsuma S

    更新日期:2016-11-01 00:00:00

  • Combination of Adt-O1Manisa and Ad5-boIFNλ3 induces early protective immunity against foot-and-mouth disease in cattle.

    abstract::Foot-and-mouth-disease (FMD) remains the most infectious livestock disease worldwide. Although commercially available inactivated or adenovirus-vectored-vaccines (Ad5-FMD) are effective, they require 5-7 days to induce protection. Therefore, new control strategies that stimulate rapid immune responses are needed. Expr...


    pub_type: 杂志文章


    authors: Diaz-San Segundo F,Montiel NA,Sturza DF,Perez-Martin E,Hickman D,Ramirez-Medina E,Grubman MJ,de Los Santos T

    更新日期:2016-12-01 00:00:00

  • Antigenic variants of bovine leukemia virus (BLV) are defined by amino acid substitutions in the NH2 part of the envelope glycoprotein gp51.

    abstract::Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...


    pub_type: 杂志文章


    authors: Portetelle D,Couez D,Bruck C,Kettmann R,Mammerickx M,Van der Maaten M,Brasseur R,Burny A

    更新日期:1989-03-01 00:00:00