The effect of inhibitors on the growth of the entomopoxvirus from Amsacta moorei in Lymantria dispar (gypsy moth) cells.


:Within the family of Poxviridae, the entomopoxviruses are the most distant relatives of the more well-known and intensively studied orthopoxviruses (vaccinia and variola). The growth of the entomopoxvirus from Amsacta moorei (AmEPV) has been characterized in cell culture and compared to that of vaccinia virus (VV), the prototypic orthopoxvirus. The overall characteristics of infected cell cultures were generally similar between the two viruses. One striking difference noted was the apparent absence of proteolytic processing of late AmEPV viral proteins, a hallmark of vertebrate poxvirus infections associated with viral morphogenesis. AmEPV, like VV, was found to be sensitive to all the inhibitors of viral infection tested including phosphonoacetic acid, 1-beta-D-arabinofuranosylcytosine, and isatin-beta-thiosemicarbazone (IBT), a compound associated with the rather specific inhibition of vertebrate poxviruses. While both VV and AmEPV are inhibited by IBT, the inhibition of AmEPV, unlike that of VV, is not accompanied by either a breakdown of ribosomal RNA or a global inhibition of late viral protein synthesis. Instead, in the presence of IBT, AmEPV enveloped, immature virions form devoid of a well-differentiated core, which unlike mature virions fail to insert into occlusion bodies.






Winter J,Hall RL,Moyer RW




Has Abstract


1995-08-20 00:00:00
















  • The complete nucleotide sequence of a New World simian foamy virus.

    abstract::We determined the complete nucleotide sequence of the New World simian foamy virus (FV) from spider monkey (SFVspm). Starting from a conserved region in the integrase (IN) domain of the pol gene we cloned fragments of the genome up to the 5' end of the long terminal repeat (LTR) into plasmid vectors and elucidated the...


    pub_type: 杂志文章


    authors: Thümer L,Rethwilm A,Holmes EC,Bodem J

    更新日期:2007-12-05 00:00:00

  • Suppression of antigen-specific T cell proliferation by measles virus infection: role of a soluble factor in suppression.

    abstract::Measles virus infection causes a profound immunosuppression. The basis for this immunosuppression is not known. This immunosuppression could be due to virus acting directly on lymphoid cells, the production of an immunosuppressive viral product, or a lymphoid product. We have developed an antigen-specific T cell syste...


    pub_type: 杂志文章


    authors: Sun X,Burns JB,Howell JM,Fujinami RS

    更新日期:1998-06-20 00:00:00

  • Increased HIV-DNA load in CCR5-negative lymphocytes without viral phenotypic change.

    abstract::We have previously described a selective increase in HIV-DNA content in CCR5-negative lymphocytes from late stage HIV-infected patients. Here, we show that this increase occurred even in the absence of viral phenotypic switching from CCR5- to CXCR4-tropic. This leads us to hypothesize that early and late CCR5-tropic v...


    pub_type: 杂志文章


    authors: Pakarasang M,Wasi C,Suwanagool S,Chalermchockcharoenkit A,Auewarakul P

    更新日期:2006-04-10 00:00:00

  • Closterovirus encoded HSP70 homolog and p61 in addition to both coat proteins function in efficient virion assembly.

    abstract::Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...


    pub_type: 杂志文章


    authors: Satyanarayana T,Gowda S,Mawassi M,Albiach-Martí MR,Ayllón MA,Robertson C,Garnsey SM,Dawson WO

    更新日期:2000-12-05 00:00:00

  • The biological phenotype of HIV-1 is usually retained during and after sexual transmission.

    abstract::The biological phenotype of HIV-1 isolates from 21 subjects with primary HIV-1 infection was determined in primary lymphocytes and monocyte-derived macrophages as well as in three T-cell lines (MT-2, HUT78, and C8166). Seven isolates (33%) replicated on at least one cell line and were classified as rapid/high (n = 6) ...


    pub_type: 杂志文章


    authors: Fiore JR,Björndal A,Peipke KA,Di Stefano M,Angarano G,Pastore G,Gaines H,Fenyö EM,Albert J

    更新日期:1994-10-01 00:00:00

  • An insertion mutation in the pol gene of Moloney murine leukemia virus results in temperature-sensitive pol maturation and viral replication.

    abstract::An insertion mutation in the pol gene of Moloney murine leukemia virus (M-MuLV) was found to render the virus temperature-sensitive for replication. A provirus containing a 12-bp insertion at the boundary between the reverse transcriptase (RT) and integrase (IN) domains induced the formation of mutant virions containi...


    pub_type: 杂志文章


    authors: Tanese N,Roth M,Epstein H,Goff SP

    更新日期:1989-06-01 00:00:00

  • Characterization of monoclonal antibodies to the Autographa californica nuclear polyhedrosis virus.

    abstract::Mouse hybridomas which produce monoclonal antibodies to the Autographa californica nuclear polyhedrosis virus (AcNPV) have been prepared and the protein to which each is directed has been determined. The antibody produced by clones 3F3 and 5E9 specifically bound polyhedron protein of MW 32,000 when used in immunoadsor...


    pub_type: 杂志文章


    authors: Roberts PL,Naser W

    更新日期:1982-10-30 00:00:00

  • The flavonoid apigenin inhibits hepatitis C virus replication by decreasing mature microRNA122 levels.

    abstract::Despite recent progress in the development of direct-acting antivirals against hepatitis C virus (HCV), chronic HCV infection remains an important health burden worldwide. MicroRNA122 (miR122), a liver-specific microRNA (miRNA), positively regulates HCV replication, and systemic application of antisense oligonucleotid...


    pub_type: 杂志文章


    authors: Shibata C,Ohno M,Otsuka M,Kishikawa T,Goto K,Muroyama R,Kato N,Yoshikawa T,Takata A,Koike K

    更新日期:2014-08-01 00:00:00

  • Molecular evolution of West Nile virus in a northern temperate region: Connecticut, USA 1999-2008.

    abstract::West Nile virus (WNV) has become firmly established in northeastern US, reemerging every summer since its introduction into North America in 1999. To determine whether WNV overwinters locally or is reseeded annually, we examined the patterns of viral lineage persistence and replacement in Connecticut over 10 consecuti...


    pub_type: 杂志文章


    authors: Armstrong PM,Vossbrinck CR,Andreadis TG,Anderson JF,Pesko KN,Newman RM,Lennon NJ,Birren BW,Ebel GD,Henn MR

    更新日期:2011-08-15 00:00:00

  • Aphidicolin-resistant DNA polymerase of bacteriophage phi 29 APHr71 mutant is hypersensitive to phosphonoacetic acid and butylphenyldeoxyguanosine 5'-triphosphate.

    abstract::Bacteriophage phi 29 DNA polymerase is sensitive to aphidicolin (APH). DNA polymerase of the APH-resistant mutant, APHr71, was more sensitive to phosphonoacetic acid and butylphenyldeoxyguanosine 5'triphosphate than the wild type. Nucleotide sequence analysis revealed a single transition of G at nucleotide 562 to A in...


    pub_type: 杂志文章


    authors: Matsumoto K,Kim CI,Kobayashi H,Kanehiro H,Hirokawa H

    更新日期:1990-09-01 00:00:00

  • Temperature-sensitive polioviruses containing mutations in RNA polymerase.

    abstract::Site-directed mutagenesis was performed to change the wild-type residue (asparagine) to aspartate, histidine, or tyrosine at amino acid 424 of the poliovirus RNA polymerase, 3Dpol. The mutations were introduced into plasmids containing full-length viral cDNA and plasmids which direct the expression of 3Dpol in Escheri...


    pub_type: 杂志文章


    authors: Burns CC,Richards OC,Ehrenfeld E

    更新日期:1992-08-01 00:00:00

  • Herpes simplex virus latent infection: reactivation and elimination of latency after neurectomy.

    abstract::Section of the sciatic nerve during the period of herpes simplex virus (HSV) latent infection was performed to evaluate residual latency in mouse dorsal root ganglion. In control mice without sciatic neurectomy, latency was present in 90-100%, while in those which underwent a neurectomy procedure, latent infection was...


    pub_type: 杂志文章


    authors: Tenser RB,Edris WA,Hay KA

    更新日期:1988-11-01 00:00:00

  • Influenza virus is not restricted by tetherin whereas influenza VLP production is restricted by tetherin.

    abstract::Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in...


    pub_type: 杂志文章


    authors: Watanabe R,Leser GP,Lamb RA

    更新日期:2011-08-15 00:00:00

  • Altered dinucleotide content within the latently transcribed regions of the DNA of alpha herpes viruses--implications for latent RNA expression and DNA structure.

    abstract::The alphaherpesviruses establish latent infections in sensory neuronal cells, during which a number of latency-associated RNA transcripts (LATs) are produced. The reasons for the production of the LATs, however, and the mechanism by which the LAT region of the genome remains active during latency are unknown. Here we ...


    pub_type: 杂志文章


    authors: Coffin RS,Howard MK,Latchman DS

    更新日期:1995-06-01 00:00:00

  • Synergistic effects on ganglionic herpes simplex virus infections by mutations or drugs that inhibit the viral polymerase and thymidine kinase.

    abstract::Herpes simplex virus encodes proteins, such as DNA polymerase, that are essential for its replication and proteins, such as thymidine kinase, that are not essential for replication in cell culture, but are important for pathogenesis in animal models. However, certain mutations affecting these proteins exert little or ...


    pub_type: 杂志文章


    authors: Jacobson J,Kramer M,Rozenberg F,Hu A,Coen DM

    更新日期:1995-01-10 00:00:00

  • Gut microbiota is associated with protection against Marek's disease virus infection in chickens.

    abstract::Marek's Disease Virus (MDV) infects chickens via respiratory route and causes lymphomas in internal organs including gastrointestinal tract. MDV infection causes a shift in the gut microbiota composition. However, interactions between the gut microbiota and immune responses against MDV infection are not well understoo...


    pub_type: 杂志文章


    authors: Bavananthasivam J,Astill J,Matsuyama-Kato A,Taha-Abdelaziz K,Shojadoost B,Sharif S

    更新日期:2021-01-15 00:00:00

  • Identification of a set of proteins (C' and C) encoded by the bicistronic P gene of the Indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral RNA polymerase.

    abstract::Previous work (C.F. Spiropoulou and S.T. Nichol, 1993, J. Virol. 67, 3103-3110) has demonstrated the existence in cells infected with the New Jersey serotype of vesicular stomatitis virus (VSV) of two small carboxy-coterminal proteins encoded by the P mRNA. These proteins have been named C' and C. We are interested in...


    pub_type: 杂志文章


    authors: Peluso RW,Richardson JC,Talon J,Lock M

    更新日期:1996-04-15 00:00:00

  • Herpes simplex virus-induced "rolling circle" amplification of SV40 DNA sequences in a transformed hamster cell line correlates with tandem integration of the SV40 genome.

    abstract::Infection with herpes simplex virus leads to amplification of SV40 DNA in various SV40-transformed cells. In earlier studies with the SV40-transformed hamster cell line Elona two different types of DNA amplification could be identified: (i) Bidirectional overreplication of chromosomally integrated SV40 DNA expanding i...


    pub_type: 杂志文章


    authors: Gerspach R,Matz B

    更新日期:1989-12-01 00:00:00

  • Recovery of structurally intact and infectious poliovirus type 1 from HeLa cells during receptor-mediated endocytosis.

    abstract::Poliovirus type 1 enters HeLa cells by receptor-mediated endocytosis as an intact virus. Up to 30 min after adsorption complete virus particles still containing VP4 and sedimenting with 156 S could be recovered from the cells. These virus particles were N-antigenic and infectious. Thirty minutes after adsorption the r...


    pub_type: 杂志文章


    authors: Willingmann P,Barnert H,Zeichhardt H,Habermehl KO

    更新日期:1989-02-01 00:00:00

  • Induction of an antiviral state by interferon in the absence of elevated levels of 2,5-oligo(A) synthetase and eIF-2 kinase.

    abstract::A series of clones has been derived from an interferon-resistant murine cell line, Ltk- aprt-, and their antiviral properties have been characterized. In the parental Ltk- aprt- line interferon is unable to establish antiviral properties or to increase the levels of 2,5-oligo(A) synthetase, the 2,5-oligo(A)-activated ...


    pub_type: 杂志文章


    authors: Lewis JA

    更新日期:1988-01-01 00:00:00

  • The AG recombinant IbNG and novel strains of group M HIV-1 are common in Cameroon.

    abstract::The genetic diversity of group M HIV-1 is highest in west central Africa. Blood samples from four locations in Cameroon were collected to determine the molecular epidemiology of HIV-1. The C2-V5 region of envelope was sequenced from 39 of the 40 samples collected, and 7 samples were sequenced across the genome. All st...


    pub_type: 杂志文章


    authors: Carr JK,Torimiro JN,Wolfe ND,Eitel MN,Kim B,Sanders-Buell E,Jagodzinski LL,Gotte D,Burke DS,Birx DL,McCutchan FE

    更新日期:2001-07-20 00:00:00

  • Neutralizing activity of antibodies to the V3 loop region of HIV-1 gp120 relative to their epitope fine specificity.

    abstract::The V3 loop of HIV-1 gp120 is considered occluded on many primary viruses. However, virus sensitivity to neutralization by different V3 mAbs often varies, indicating that access to V3 is not restricted equally for all antibodies. Here, we have sought to gain a better understanding of these restrictions by determining ...


    pub_type: 杂志文章


    authors: Pantophlet R,Wrin T,Cavacini LA,Robinson JE,Burton DR

    更新日期:2008-11-25 00:00:00

  • Comparison of viral replication and IFN response in alpaca and bovine cells following bovine viral diarrhea virus infection.

    abstract::Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissivene...


    pub_type: 杂志文章


    authors: Samson HC,Topliff CL,Donis RO,Kelling CL

    更新日期:2011-04-25 00:00:00

  • Immunity to rotavirus in T cell deficient mice.

    abstract::Rotavirus infection was studied in adult nude mice (BALB/c background), alpha beta or gamma delta and alpha beta/gamma delta T cell receptor (TCR) knockout (-/-) mice (C57BL/6 and C57BL/6 x 129 backgrounds), and SCID mice (C57BL/6 background). The gamma delta TCR -/- mice cleared infection just like control mice. All ...


    pub_type: 杂志文章


    authors: Franco MA,Greenberg HB

    更新日期:1997-11-24 00:00:00

  • Molecular dissection of Flock house virus protein B2 reveals that electrostatic interactions between N-terminal domains of B2 monomers are critical for dimerization.

    abstract::Flock house virus (FHV) encodes a suppressor protein B2 to overcome antiviral RNA silencing during infection. Biochemical analyses have shown that a homodimer of B2 binds to double-stranded RNA to inhibit dicer-mediated cleavage of dsRNA and incorporation of small interfering RNAs into the RNA-induced silencing comple...


    pub_type: 杂志文章


    authors: Seo JK,Kwon SJ,Rao AL

    更新日期:2012-10-25 00:00:00

  • Mutational analysis of early region 4 of bovine adenovirus type 3.

    abstract::The primary objective of characterizing bovine adenovirus type 3 (BAV3) in greater detail is to develop it as a vector for gene therapy and vaccination of humans and animals. A series of BAV3 early region 4 (E4) deletion-mutant viruses, containing deletions in individual E4 open reading frames (Orf) or combinations of...


    pub_type: 杂志文章


    authors: Baxi MK,Robertson J,Babiuk LA,Tikoo SK

    更新日期:2001-11-10 00:00:00

  • Identification of tolerated insertion sites in poliovirus non-structural proteins.

    abstract::Insertion of nucleotide sequences encoding "tags" that can be expressed in specific viral proteins during an infection is a useful strategy for purifying viral proteins and their functional complexes from infected cells and/or for visualizing the dynamics of their subcellular location over time. To identify regions in...


    pub_type: 杂志文章


    authors: Teterina NL,Lauber C,Jensen KS,Levenson EA,Gorbalenya AE,Ehrenfeld E

    更新日期:2011-01-05 00:00:00

  • One hundred years of poliovirus pathogenesis.

    abstract::Poliovirus was first isolated nearly 100 years ago in a landmark experiment that established the viral etiology of poliomyelitis. This discovery stimulated investigation of the pathogenesis of poliomyelitis in many laboratories. Nearly 50 years later, when two effective poliovirus vaccines were developed, the impetus ...


    pub_type: 杂志文章,评审


    authors: Racaniello VR

    更新日期:2006-01-05 00:00:00

  • Effect of Rev on the intranuclear localization of HIV-1 unspliced RNA.

    abstract::Human immunodeficiency virus type 1 (HIV-1) Rev is a 19-kDa regulatory protein which binds to unspliced and partially spliced HIV-1 RNAs. Export, splicing, stability, and translation of HIV-1 RNAs are influenced by Rev. To further understand the effect of Rev on HIV-1 RNA splicing, the intranuclear localization of uns...


    pub_type: 杂志文章


    authors: Favaro JP,Borg KT,Arrigo SJ,Schmidt MG

    更新日期:1998-09-30 00:00:00

  • Different HIV pox viral vector-based vaccines and adjuvants can induce unique antigen presenting cells that modulate CD8 T cell avidity.

    abstract::The lung-derived dendritic cell (LDC) recruitment following intranasal (i.n.) vaccination of different poxviral vector-based vaccines/adjuvants were evaluated to decipher how these factors influenced CD8(+) T cell avidity. Compared to the standard i.n. recombinant fowlpox virus (FPV)-HIV vaccination, the FPV-HIV IL-13...


    pub_type: 杂志文章


    authors: Trivedi S,Jackson RJ,Ranasinghe C

    更新日期:2014-11-01 00:00:00