Abstract:
:Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mori cultured cells and larvae, although viral genome replication was not affected. Bm5 disruption also caused aberrant expression of various viral genes at the very late stage of infection. Immunocytochemical analysis revealed that BM5 localized to the nuclear membrane. We also found that DUF3627 is important for OB production, transcriptional regulation of viral genes, and subcellular localization of BM5. Compared with wild-type BmNPV infection, larval death was delayed when B. mori larvae were infected with Bm5 mutants. These results suggest that BM5 is involved in progeny virus production and regulation of viral gene expression at the very late stage of infection.
journal_name
Virologyjournal_title
Virologyauthors
Kokusho R,Koh Y,Fujimoto M,Shimada T,Katsuma Sdoi
10.1016/j.virol.2016.08.032subject
Has Abstractpub_date
2016-11-01 00:00:00pages
240-249eissn
0042-6822issn
1096-0341pii
S0042-6822(16)30248-3journal_volume
498pub_type
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