Abstract:
:Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phage DNA, and reduced genetic recombination. The lethal effect of gene 3.5 or 4 mutations is suppressed when the sup0 bacteria express the RecE recombination pathway. This RecE suppression occurs by partial restoration of the capacity to make concatemeric molecules and partial reversal of the DNA arrest defect which, in turn, leads to the formation of viable progeny. Infection by T1+ or by mutants defective in any of the four DNA synthesis genes (genes 1, 2, 3.5, and 4) inhibited the ATP-dependent exonuclease present in uninfected cells (presumably the RecBC enzyme, exonuclease V). Extracts from T1+ infections also showed increased levels of an ATP-independent exonuclease activity which was absent from gene 4 mutant extracts. It is concluded that gene 4, together with gene 3.5, specifies an activity related to that of the RecE exonuclease VIII and essential for T1 concatemer formation and recombination.
journal_name
Virologyjournal_title
Virologyauthors
Pugh JC,Ritchie DAdoi
10.1016/0042-6822(84)90130-2subject
Has Abstractpub_date
1984-05-01 00:00:00pages
200-6issue
1eissn
0042-6822issn
1096-0341journal_volume
135pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Three distinct species of nucleocapsids of equine herpesvirus type-1, designated as either light (L), intermediate (I), or heavy (H) on the basis of their densities in Renografin-76 density gradients (Perdue et al. 1975), were characterized with respect to their amino acid content, DNA content, and role in the maturat...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::Spindle-shaped viruses are a dominant morphotype in hypersaline waters but their molecular characteristics and their relationship to other archaeal viruses have not been determined. Here, we describe the isolation, characteristics and genome sequence of His2, a spindle-shaped halovirus, and compare it to the previousl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.02.005
更新日期:2006-06-20 00:00:00
abstract::An infectious raccoon poxvirus (RCNV) was used to express the feline panleukopenia virus (FPV) open reading frame VP2. The recombinant, RCNV/FPV, was constructed by homologous recombination with a chimeric plasmid for inserting the expression cassette into the thymidine kinase (TK) locus of RCNV. Expression of the VP2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0186
更新日期:1996-04-01 00:00:00
abstract::Due to the worldwide prevalence of antibiotic resistant strains, phages therapy has been revitalized recently. In this study, an Enterococcus faecium phage named IME-EFm5 was isolated from hospital sewage. Whole genomic sequence analysis demonstrated that IME-EFm5 belong to the Siphoviridae family, and has a double-st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.02.006
更新日期:2016-05-01 00:00:00
abstract::Potato virus S (PVS) is a major plant pathogen that causes considerable losses in global potato production. Knowledge of the evolutionary history and spatio-temporal dynamics of PVS is vital for developing sustainable management schemes. In this study, we investigated the phylodynamics of the virus by analysing 103 nu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.09.022
更新日期:2018-12-01 00:00:00
abstract::The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90439-3
更新日期:1986-10-15 00:00:00
abstract::Defective interfering particles of New Jersey serotype VSV were found to contain very little or no virion transcriptase activity. Furthermore no VSV transcriptase activity was observed in cells treated with cycloheximide and infected with DI. Cells infected with DI together with standard infectious virus and cyclohexi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(72)90356-x
更新日期:1972-10-01 00:00:00
abstract::Previously, an epitope recognized by a set of neutralizing monoclonal antibodies directed against the S protein of transmissible gastroenteritis has been identified. This neutralization epitope can be simulated by a single peptide combining residues 380 to 387 and 1176 to 1184 of the S protein; this combination peptid...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90684-4
更新日期:1991-05-01 00:00:00
abstract::Encephalitis is a rare, but serious complication from vaccination against smallpox using replication competent strains of vaccinia virus. In this report we describe mutants of vaccinia virus, containing N-terminal deletions of the vaccinia virus interferon resistance gene, E3L, that are attenuated for neuropathogenesi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.006
更新日期:2005-03-15 00:00:00
abstract::Computational analysis of human adenovirus type 4 (HAdV-E4), a pathogen that is the only HAdV member of species E, provides insights into its zoonotic origin and molecular adaptation. Its genome encodes a domain of the major capsid protein, hexon, from HAdV-B16 recombined into the genome chassis of a simian adenovirus...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.05.014
更新日期:2013-09-01 00:00:00
abstract::Herpes simplex virus encodes proteins, such as DNA polymerase, that are essential for its replication and proteins, such as thymidine kinase, that are not essential for replication in cell culture, but are important for pathogenesis in animal models. However, certain mutations affecting these proteins exert little or ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80041-7
更新日期:1995-01-10 00:00:00
abstract::Two forms of JC virus (JCV) have been isolated from its human host, an archetype found in kidney tissue and urine of nonimmunocompromised individuals and a rearranged type detected in lymphocytes and brain tissue of patients with and without progressive multifocal leukoencephalopathy. To investigate the hypothesis tha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0037
更新日期:1996-02-01 00:00:00
abstract::Multibranched peptides (SPCs) derived either from the fusion protein (gp41) sequence or from the cleavage sequence of the human immunodeficiency virus type 1 envelope were chemically synthesized and tested for their ability to inhibit both syncytium formation and HIV production in CD4+ cells. The gp41-derived SPCs had...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0496
更新日期:1996-09-15 00:00:00
abstract::Seven structural component proteins of a paramyxovirus simian virus 41 (SV41) were identified with the aid of monoclonal antibodies prepared against SV41 and human parainfluenza type 2 virus (PIV2). The nucleoprotein is antigenically very close to that of PIV2, while it is comparatively far from that of simian virus 5...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90141-d
更新日期:1990-12-01 00:00:00
abstract::HeLa cell extracts containing wild-type copies of the minute virus of mice NS-1 polypeptide produced from a recombinant vaccinia virus vector could support the excision and replication of viral 5' telomeres from cloned concatemer junction fragments. Resolution did not occur if wild-type NS-1 was omitted from the extra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91223-h
更新日期:1992-09-01 00:00:00
abstract::Cultured primary peripheral blood mononuclear cells (PBMC) represent a potentially physiologic in vitro model of HIV-1 infection, but assessment of antibody-mediated HIV-1 neutralization using PBMC has been hindered by donor variability and lack of a sustainable individual PBMC source. To advance this model for HIV va...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.09.014
更新日期:2013-12-01 00:00:00
abstract::Inflammasome activation is gaining recognition as an important mechanism for protection during viral infection. Here, we investigate whether Rift Valley fever virus, a negative-strand RNA virus, can induce inflammasome responses and IL-1β processing in immune cells. We have determined that RVFV induces NLRP3 inflammas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.11.015
更新日期:2014-01-20 00:00:00
abstract::Transcription of human papillomaviruses (HPV) is regulated by enhancer sequences located in the upstream regulatory region. The factors regulating expression of one of the high risk genital HPV types, HPV 31b, were investigated using transient expression and protein binding assays. A region of 262 base pairs in length...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1390
更新日期:1995-08-01 00:00:00
abstract::The major late promoter (MLP) of the subgroup C human adenoviruses is a preeminent model for the study of the mechanisms of basal and activated transcription, both in vivo and in vitro. However, while the structure and function of the human virus MLP has been the subject of extensive investigation, the conservation of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0327
更新日期:1996-06-15 00:00:00
abstract::Turnip leaves infected with turnip crinkle virus accumulate a 35-kd polypeptide which comigrates with the major protein from isolated virions. RNA from TCV virions directs the synthesis of a number of polypeptides in vitro including a 35-kd protein which is immunoprecipitable with antiserum to virus particles. Transla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90326-9
更新日期:1982-04-15 00:00:00
abstract::To study the process of membrane fusion in Morbilliviruses, the fusion (F) glycoproteins of Peste des petits ruminants virus (PPRV) and Rinderpest virus (RPV) were expressed transiently in mammalian cells. The recombinant F proteins were found to be localized at the surface of transfected cells. The fusion activity, a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1120
更新日期:2001-10-10 00:00:00
abstract::A cDNA clone containing all of the 26 S mRNA coding region of the RNA genome of Venezuelan equine encephalitis (VEE) virus, virulent strain Trinidad donkey (TRD), has been constructed and sequenced. The nucleotide and deduced amino acid sequences of the 26 S RNA of VEE virus conform to the general organization of the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90142-x
更新日期:1986-07-30 00:00:00
abstract::Glycoprotein B (gB, gpUL55) is a major antigen for the induction of neutralizing antibodies against human cytomegalovirus, making it an attractive antigen for active and passive immunoprophylaxis. The immunodominant region on gB is the antigenic domain 1 (AD-1), a complex structure which requires a minimal linear amin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0040
更新日期:1996-02-01 00:00:00
abstract::The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species termed RNA-1 and RNA-2. RNA-2 is required for infection of whole plants but is dispensable for infection and virion formation in protoplasts. We have used full-length cDNA clones of RNA-1 and -2 from which infec...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1004
更新日期:1993-01-01 00:00:00
abstract::Crystals of Cowpea mosaic virus displaying a high degree of order have been grown in two different forms. Hexagonal crystals, space group P6(1)22 or P6(5)22 with a = 450 A and c = 1038 A containing six virus particles per cell, have been grown in sodium citrate buffer at pH 4.9. Each particle has four nearest neighbor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90513-9
更新日期:1980-02-01 00:00:00
abstract::The bovine herpesvirus 1 (BHV-1) U(S) ORF8 protein with homology to the Us9 protein of other alphaherpesviruses induces apoptosis in rabbit kidney (RK13) cells without the presence of other BHV-1-encoded proteins. In this article, we have characterized the cytotoxicity and growth behavior of a BHV-1 recombinant, BHV-1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1656
更新日期:2002-12-05 00:00:00
abstract::The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BKV exists as archetype virus and rearranged variants, w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.08.032
更新日期:2010-11-25 00:00:00
abstract::Rabies continues to poses serious threats to the public health in many countries. The development of novel inexpensive, safe and effective vaccines has become a high priority for rabies control worldwide. We previously generated a novel recombinant rabies vaccine by cloning rabies virus glycoprotein into a chimpanzee ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.07.022
更新日期:2019-10-01 00:00:00
abstract::The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epith...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.02.054
更新日期:2015-08-01 00:00:00
abstract::The V protein of Sendai virus (SeV) is nonessential for virus replication in cell culture but indispensable for viral pathogenicity in mice. At the C terminus of the V protein, there are amino acid residues conserved among the members of the Paramyxovinae subfamily that are clustered in three regions: region I, just d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1516
更新日期:2002-08-01 00:00:00