Abstract:
:To study the process of membrane fusion in Morbilliviruses, the fusion (F) glycoproteins of Peste des petits ruminants virus (PPRV) and Rinderpest virus (RPV) were expressed transiently in mammalian cells. The recombinant F proteins were found to be localized at the surface of transfected cells. The fusion activity, as evident from cell fusion assays and lysis of chicken erythrocytes, documented that transiently expressed PPRV F glycoprotein induces cell fusion in the absence of homotypic hemagglutinin-neuraminidase (HN) attachment glycoprotein. The coexpression of homotypic HN increased the extent of fusion by twofold, while the efficiency of fusion was found to be substantially enhanced. In contrast, in RPV F-expressing cells, fusion was detected only when homotypic hemagglutinin (H) or heterotypic HN protein was coexpressed. This differs from the strict type-specific requirement for the attachment protein as in the fusion process of most of the paramyxoviruses. Further, we demonstrate by fluorescence transfer experiments that while PPRV F brings about both hemifusion and complete fusion on its own, RPV F induces only hemifusion while it brings about complete fusion in the presence of homotypic or heterotypic attachment protein.
journal_name
Virologyjournal_title
Virologyauthors
Seth S,Shaila MSdoi
10.1006/viro.2001.1120subject
Has Abstractpub_date
2001-10-10 00:00:00pages
86-94issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(01)91120-1journal_volume
289pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Viruses were recovered from HeLa S3 cells and African green monkey kidney (AGMK) cells transfected with an infectious cDNA clone of poliovirus vaccine Sabin 1 strain. The viruses recovered from the different DNA-transfected cells were tested for the biological characteristics of temperature sensitivity (rct marker), p...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90100-5
更新日期:1986-05-01 00:00:00
abstract::Tombusviruses are small, plus-sense, single-stranded RNA viruses of plants. RNA-dependent RNA polymerases (RdRp) of two tombusviruses, Tomato bushy stunt virus (TBSV) and Cucumber necrosis virus (CNV), have been partially purified from infected Nicotiana benthamiana plants. The obtained RdRp complexes are capable of d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0577
更新日期:2000-10-25 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 is targeted by broadly-reactive neutralizing antibodies 2F5 and 4E10, making it an attractive target for vaccine development. To better assess immunogenic properties of gp41, we generated five soluble glutathione S-transferase fusion proteins ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.11.009
更新日期:2008-03-15 00:00:00
abstract::The identification of mechanisms that prevent infection with human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV) would facilitate the development of an effective AIDS vaccine. In time-course experiments, protection against detectable superinfection with homologous wild-type SIV was achieved withi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.09.026
更新日期:2004-12-05 00:00:00
abstract::Cauliflower mosaic virus (CaMV) uses a specialised translation mechanism to bypass the long leader sequence of the 35S RNA. The effect of the CaMV 35S RNA leader sequence on the expression of a downstream beta-glucuronidase (GUS) reporter gene was studied in transgenic tobacco plants. Enzymatic GUS assays of these tra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9038
更新日期:1998-03-15 00:00:00
abstract::A human B-lymphoid cell line, designated TG8, which does not express detectable levels of the EBV (Epstein-Barr virus)-associated nuclear antigen (EBNA), yet carries an average of one to two plasmid copies of the P3HR-1 EBV genome has been identified. TG8 can be superinfected by B95-8 EBV, resulting in up to 60-70% of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90014-x
更新日期:1984-08-01 00:00:00
abstract::The host-virus interaction during the cellular entry of Japanese encephalitis virus (JEV) is poorly characterized. The ubiquitin-proteasome system (UPS), the major intracellular proteolytic pathway, mediates diverse cellular processes, including endocytosis and signal transduction, which may be involved in the entry o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.08.013
更新日期:2016-11-01 00:00:00
abstract::The effect of murine cytomegalovirus (MCMV) infection on interleukin 1 (IL-1) secretion was assessed using the macrophage cell lines P388D1 and J774A.1. The former proved to be nonpermissive for MCMV in that infectious virus and viral immediate early protein (pp89) were not expressed in these cells. MCMV infection of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90342-o
更新日期:1990-10-01 00:00:00
abstract::In wild-type human parainfluenza virus type 2 (WT HPIV2), one gene (the P/V gene) encodes both the polymerase-associated phosphoprotein (P) and the accessory V protein. We generated a HPIV2 virus (rHPIV2-V(ko)) in which the P/V gene encodes only the P protein to examine the role of V in replication in vivo and as a po...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.018
更新日期:2010-02-20 00:00:00
abstract::This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90172-4
更新日期:1989-04-01 00:00:00
abstract::Chimeric proteins consisting of the VP2 capsid protein of human parvovirus B19 and defined linear epitopes from human herpes simplex virus type 1 and mouse hepatitis virus A59 inserted at the N-terminus and at a predicted surface region were expressed by recombinant baculoviruses. The chimeric proteins expressed the i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1059
更新日期:1994-02-01 00:00:00
abstract::Three prophage sequences were identified in the Lactobacillus johnsoni strain NCC533. Prophage Lj965 predicted a gene map very similar to those of pac-site Streptococcus thermophilus phages over its DNA packaging and head and tail morphogenesis modules. Sequence similarity linked the putative DNA packaging and head mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0498
更新日期:2000-09-30 00:00:00
abstract::Coordinated expression of viral genes in primary infection is essential for successful infection of host cells. We examined the expression profiles of Kaposi's sarcoma-associated herpesvirus (KSHV) transcripts in productive primary infection of primary human umbilical vein endothelial cells by whole-genome reverse-tra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.08.018
更新日期:2005-12-05 00:00:00
abstract::Intact clones containing partial repeats of the genomic components of African cassava mosaic (ACMV DNAs A and B) are infectious when mechanically coinoculated onto Nicotiana benthamiana. Monomeric genomic components may be generated either by homologous recombination or, when two copies of the origin of replication (o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80093-x
更新日期:1995-01-10 00:00:00
abstract::Herpes simplex virus mutant KG111 contains a nonsense mutation at codon 44 of the viral thymidine kinase (tk) gene and produces low amounts of a truncated tk polypeptide. We tested mutant KG111 and related viruses that specify varying amounts of similar truncated tk polypeptides for their sensitivities to antiviral nu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90261-4
更新日期:1989-02-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) glycoprotein B (gB) is a multifunctional glycoprotein required for infectivity; it is thought to promote fusion of the viral envelope with the cell membrane and entry of virions into cells. To map the antigenic and functional domains on gB, we constructed amino terminal derivatives lacki...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90102-5
更新日期:1989-09-01 00:00:00
abstract::Plasmid p12929 was shown to integrate into the chromosome of Corynebacterium glutamicum RM3 and BL15. The minimal integrating fragment was subsequently defined. The arms flanking the integrated plasmid (attL and attR) were identified, allowing for the determination of the attP and the attB attachment sites. The attB s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9524
更新日期:1999-03-01 00:00:00
abstract::Two small viral proteins (DGBp1 and DGBp2) have been proposed to act in a concerted manner to aid intra- and intercellular trafficking of carmoviruses though the distribution of functions and mode of action of each protein partner are not yet clear. Here we have confirmed the requirement of the DGBps of Pelargonium fl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.001
更新日期:2011-05-10 00:00:00
abstract::The mitotic spindle assembly checkpoint (SAC) ensures faithful chromosome segregation during mitosis by censoring kinetochore-microtubule interactions. It is frequently rendered dysfunctional during carcinogenesis causing chromosome missegregation and genomic instability. There are conflicting reports whether the HPV1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.06.006
更新日期:2012-10-10 00:00:00
abstract::Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discov...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9893
更新日期:1999-10-10 00:00:00
abstract::NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1059
更新日期:1993-02-01 00:00:00
abstract::Processing of the transmembrane glycoprotein (GP) of Marburg virus involved the conversion of an endo H-sensitive, ER-specific form into an endo H-resistant, Golgi-specific precursor that was cleaved into GP(1) and GP(2). Cleavage was mediated by furin or another subtilisin-like endoprotease with similar substrate spe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0110
更新日期:2000-03-01 00:00:00
abstract::The tail sheath protein of giant bacteriophage phiKZ Pseudomonas aeruginosa encoded by gene 29 was identified and its expression system was developed. Localization of the protein on the virion was confirmed by immunoelectron microscopy. Properties of gene product (gp) 29 were studied by electron microscopy, immunoblot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.09.015
更新日期:2009-12-20 00:00:00
abstract::In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage mat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90273-9
更新日期:1985-07-30 00:00:00
abstract::Picornavirus infection induces the proliferation and rearrangement of intracellular membranes in response to the synthesis of nonstructural proteins, including 3A. We have previously shown that changes in 3A are associated with the inability of a Taiwanese strain of foot-and-mouth disease virus (FMDV) (OTai) to grow i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1035
更新日期:2001-08-15 00:00:00
abstract::A recombinant vaccinia virus that expresses the nucleoprotein gene of Lassa virus (Josiah strain) under the control of the P7.5 promoter was constructed using the lacZ coexpression transfer vector pSC11. Southern blot analysis demonstrated that recombination of the sequences inserted within the thymidine kinase gene o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90525-4
更新日期:1989-07-01 00:00:00
abstract::Turnip leaves infected with the aphid transmissible isolate of cauliflower mosaic virus (CaMV Cabb B-JI) showed two types of virus-containing inclusion bodies (IBs), which differed morphologically and in their protein composition when analyzed by immunogold labeling of ultrathin sections. Vacuolated IBs, typical of Ca...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90781-6
更新日期:1991-11-01 00:00:00
abstract::HIV CRF07 B'/C is a strain circulating mainly in northwest region of China. The gp41 region of CRF07 is derived from a clade C virus. In order to compare the difference of CRF07 gp41 with that of typical clade B virus, we solved the crystal structure of the core region of CRF07 gp41. Compared with clade B gp41, CRF07 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.07.024
更新日期:2013-11-01 00:00:00
abstract::Pandemic H1N1 influenza A (H1N1pdm) elicits stronger pulmonary inflammation than previously circulating seasonal H1N1 influenza A (sH1N1), yet mechanisms of inflammatory activation in respiratory epithelial cells during H1N1pdm infection are unclear. We investigated host responses to H1N1pdm/sH1N1 infection and virus ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.09.022
更新日期:2014-01-05 00:00:00
abstract::Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90283-1
更新日期:1985-07-30 00:00:00