当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Domain structure of herpes simplex virus 1 glycoprotein B: neutralizing epitopes map in regions of continuous and discontinuous residues.

Domain structure of herpes simplex virus 1 glycoprotein B: neutralizing epitopes map in regions of continuous and discontinuous residues.

Abstract：

:Herpes simplex virus 1 (HSV-1) glycoprotein B (gB) is a multifunctional glycoprotein required for infectivity; it is thought to promote fusion of the viral envelope with the cell membrane and entry of virions into cells. To map the antigenic and functional domains on gB, we constructed amino terminal derivatives lacking the entire carboxyl terminus and internal deletion mutants lacking defined regions of the extracellular and transmembrane domains. Transient expression of the mutants in COS-1 cells revealed that the amino terminal derivatives were released into the medium whereas those with deletions in the extracellular domain were mostly retained within the transfected cells. Analysis of intact gB and the amino terminal derivatives showed that the intact molecule formed dimers whereas the mutant derivatives did not. Reactions of the derivatives with a panel of well-characterized monoclonal antibodies to gB showed that the neutralizing epitopes cluster in two domains. The first maps in the amino terminal 190 residues and contains seven continuous epitopes, five of which are HSV-1-specific. Reactions of antibodies with a set of oligopeptides fine-mapped the epitopes between residues 1 and 47. The second domain is composed of discontinuous epitopes and was expressed by amino terminal derivatives that were at least 457 residues in length or longer. Eleven epitopes map in this region, including those of four potent neutralizing antibodies whose cognitive sites mapped between residues 273 and 298 in mapping studies using antibody-resistant mutants. Results of the present study indicate that the cognitive sites of these antibodies are assembled into the discontinuous domain by juxtaposing residues from the amino-terminal half of gB monomers.

journal_name

Virology

journal_title

Virology

authors

Pereira L,Ali M,Kousoulas K,Huo B,Banks T

doi

10.1016/0042-6822(89)90102-5

subject

Has Abstract

pub_date

1989-09-01 00:00:00

pages

11-24

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

172

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Antibody response in the female rabbit reproductive tract to influenza haemagglutinin encoded by a recombinant myxoma virus.

    abstract：:The antibody response in serum and the reproductive tract of female rabbits to a model antigen, influenza virus haemagglutinin (HA), encoded by a recombinant myxoma virus was investigated. Strong and lasting IgG antibody responses to HA were induced in serum following intradermal, intranasal, and intravaginal immunisa...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00324-6

    authors： Gu W,Holland M,Janssens P,Kerr P

    更新日期：2003-08-15 00:00:00

  • Crystalline cowpea mosaic virus.

    abstract：:Crystals of Cowpea mosaic virus displaying a high degree of order have been grown in two different forms. Hexagonal crystals, space group P6(1)22 or P6(5)22 with a = 450 A and c = 1038 A containing six virus particles per cell, have been grown in sodium citrate buffer at pH 4.9. Each particle has four nearest neighbor...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(80)90513-9

    authors： White JM,Johnson JE

    更新日期：1980-02-01 00:00:00

  • Effects of partially dismantling the CD4 binding site glycan fence of HIV-1 Envelope glycoprotein trimers on neutralizing antibody induction.

    abstract：:Previously, VLPs bearing JR-FL strain HIV-1 Envelope trimers elicited potent neutralizing antibodies (nAbs) in 2/8 rabbits (PLoS Pathog 11(5): e1004932) by taking advantage of a naturally absent glycan at position 197 that borders the CD4 binding site (CD4bs). In new immunizations, we attempted to improve nAb response...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.02.024

    authors： Crooks ET,Osawa K,Tong T,Grimley SL,Dai YD,Whalen RG,Kulp DW,Menis S,Schief WR,Binley JM

    更新日期：2017-05-01 00:00:00

  • Functional simian immunodeficiency virus Gag-specific CD8+ intraepithelial lymphocytes in the mucosae of SIVmac251- or simian-human immunodeficiency virus KU2-infected macaques.

    abstract：:The vaginal and rectal mucosae are the first line of cellular immune defense to sexually transmitted human immunodeficiency virus type 1 (HIV-1) entry. Thus, intraepithelial lymphocytes (IELs) may be important in the immune response to HIV infection. Here we investigated whether functional IELs in mucosal compartments...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.08.046

    authors： Stevceva L,Moniuszko M,Alvarez X,Lackner AA,Franchini G

    更新日期：2004-02-20 00:00:00

  • Microevolution of tick-borne encephalitis virus in course of host alternation.

    abstract：:Two tick-borne encephalitis (TBE) virus variants were studied: mouse brain-adapted strain EK-328 and its derivate adapted to Hyalomma marginatum ticks. The tick-adapted virus exhibited small-plaque phenotype and slower replication in PEK cells, higher yield in ticks, decreased neuroinvasiveness in mice, increased bind...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.12.013

    authors： Romanova LIu,Gmyl AP,Dzhivanian TI,Bakhmutov DV,Lukashev AN,Gmyl LV,Rumyantsev AA,Burenkova LA,Lashkevich VA,Karganova GG

    更新日期：2007-05-25 00:00:00

  • Myb protein binds to multiple sites in the human T cell lymphotropic virus type 1 long terminal repeat and transactivates LTR-mediated expression.

    abstract：:The members of the c-myb proto-oncogene family encode sequence-specific transcriptional activators. In T cells, expression of c-myb and the related B-myb gene is induced following mitogenic stimulation. Using a purified recombinant protein, we report here that the human T cell lymphotropic virus type 1 (HTLV-1) LTR c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90044-p

    authors： Bosselut R,Lim F,Romond PC,Frampton J,Brady J,Ghysdael J

    更新日期：1992-02-01 00:00:00

  • The minute virus of mice exploits different endocytic pathways for cellular uptake.

    abstract：:The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epith...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.02.054

    authors： Garcin PO,Panté N

    更新日期：2015-08-01 00:00:00

  • Isolation of mutations of the phage Mu ner gene.

    abstract：:A method was devised which allows the easy detection of mutations within the ner gene of Mu DNA. This method is based upon the observation that a transcriptional gene A-galK fusion containing the complete ner gene and the cts62 allele does not express the galK gene in an Escherichia coli strain lacking functional inte...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90621-6

    authors： König U,Kirschner P,Schumann W

    更新日期：1988-05-01 00:00:00

  • The internal polyadenylate tract of yeast killer virus M1 double-stranded RNA is variable in length.

    abstract：:The 1.8-kbp M1 double-stranded (ds) RNA from type 1 killer strains of Saccharomyces cerevisiae contains an internal 200-bp adenine- and uracil-rich region. We have previously demonstrated that this region consists primarily of adenine residues on the plus strand of M1 dsRNA and on the full-length, in vitro synthesized...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90380-6

    authors： Hannig EM,Williams TL,Leibowitz MJ

    更新日期：1986-07-15 00:00:00

  • Identification of two new polypeptides encoded by mRNA5 of the coronavirus infectious bronchitis virus.

    abstract：:The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two poly...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90094-6

    authors： Liu DX,Inglis SC

    更新日期：1992-01-01 00:00:00

  • Mapping of linear antigenic sites on the S glycoprotein of a neurotropic murine coronavirus with synthetic peptides: a combination of nine prediction algorithms fails to identify relevant epitopes and peptide immunogenicity is drastically influenced by th

    abstract：:The elucidation of the antigenic structure of the S glycoprotein of murine coronaviruses will provide further understanding of the complex pathogenicity of these viruses. In order to identify linear antigenic determinants, the primary structure of the S glycoprotein of murine hepatitis virus strain A59 was analyzed wi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1376

    authors： Daniel C,Lacroix M,Talbot PJ

    更新日期：1994-08-01 00:00:00

  • The Rice stripe virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana.

    abstract：:The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis i...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.01.007

    authors： Zhang C,Pei X,Wang Z,Jia S,Guo S,Zhang Y,Li W

    更新日期：2012-04-10 00:00:00

  • Complementation analysis of the dales collection of vaccinia virus temperature-sensitive mutants.

    abstract：:A collection of randomly generated temperature-sensitive (ts) vaccinia virus (strain IHD-W) mutants were reported by S. Dales et al., (1978, Virology, 84, 403-428) in 1978 and characterized by electron microscopy. We have performed further genetic analysis on the Dales collection of mutants to make the mutants more us...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1745

    authors： Lackner CA,D'Costa SM,Buck C,Condit RC

    更新日期：2003-01-20 00:00:00

  • The gag and pol genes of bovine leukemia virus: nucleotide sequence and analysis.

    abstract：:The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode on...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90344-7

    authors： Rice NR,Stephens RM,Burny A,Gilden RV

    更新日期：1985-04-30 00:00:00

  • SARS coronavirus 7a protein blocks cell cycle progression at G0/G1 phase via the cyclin D3/pRb pathway.

    abstract：:The genome of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) contains four structural genes that are homologous to genes found in other coronaviruses, and also contains six subgroup-specific open reading frames (ORFs). Expression of one of these subgroup-specific genes, ORF7a, resulted in apoptosi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.10.015

    authors： Yuan X,Wu J,Shan Y,Yao Z,Dong B,Chen B,Zhao Z,Wang S,Chen J,Cong Y

    更新日期：2006-03-01 00:00:00

  • Genetic identification of a portion of the herpes simplex virus ICP8 protein required for DNA-binding.

    abstract：:The major DNA-binding protein or infected cell protein 8 (ICP8) encoded by herpes simplex virus exhibits multiple interactions with the cell nucleus in that it interacts with the host cell nuclear matrix and viral DNA molecules as sequential stages in its maturational process (M. P. Quinlan, L. B. Chen, and D. M. Knip...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90272-3

    authors： Gao M,Bouchey J,Curtin K,Knipe DM

    更新日期：1988-04-01 00:00:00

  • Antibodies that neutralize SIV(mac)251 in T lymphocytes cause interruption of the viral life cycle in macrophages by preventing nuclear import of viral DNA.

    abstract：:Previous reports from our lab had shown that sera obtained from SIV(mac)-infected animals neutralized SIV(mac) infectivity in CD4(+) T cells but failed to protect monkey primary macrophages from infection with the virus. However, the antibodies could inhibit completion of the viral life cycle in the macrophages at the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1053

    authors： Zhuge W,Jia F,Mackay G,Kumar A,Narayan O

    更新日期：2001-09-01 00:00:00

  • The Helminthosporium victoriae 190S mycovirus has two forms distinguishable by capsid protein composition and phosphorylation state.

    abstract：:Purified preparations of the Helminthosporium victoriae 190S (Hv190S) virus contain two sedimenting components that differ in capsid structure. The slower sedimenting component (190S-1) contained p88 and p83 as the major capsid proteins; the faster component (190S-2) contained p88 and p78. Previous peptide-mapping stu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90520-y

    authors： Ghabrial SA,Havens WM

    更新日期：1992-06-01 00:00:00

  • Interferon-λ4 is a cell-autonomous type III interferon associated with pre-treatment hepatitis C virus burden.

    abstract：:Genetic variants surrounding the interferon-λ3 (IFNL3) gene are strongly associated with clearance of hepatitis C virus (HCV). A variant (rs368234815 TT/ΔG) upstream of IFNL3 was recently implicated to control expression of a novel gene termed IFNL4. We conducted genetic analysis of rs368234815 in a chronic HCV patien...

    journal_title：Virology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.virol.2014.12.020

    authors： Lu YF,Goldstein DB,Urban TJ,Bradrick SS

    更新日期：2015-02-01 00:00:00

  • Restriction of a Sindbis virus mutant in BHK cells and relief of the restriction by the addition of adenosine.

    abstract：:SV(PZF) is a mutant of Sindbis virus (SV) which we selected on the basis of its ability to replicate in mosquito cells treated with pyrazofurin (PZF), a drug which inhibits pyrimidine nucleotide biosynthesis (Lin et al., 2000, Virology 272, 61-71). Three mutations, A6627U, A7543U, and C7593A, were identified in the ns...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1230

    authors： Lin YH,Simmonds HA,Stollar V

    更新日期：2002-01-05 00:00:00

  • Neuronal IFN signaling is dispensable for the establishment of HSV-1 latency.

    abstract：:IFN responses control acute HSV infection, but their role in regulating HSV latency is poorly understood. To address this we used mice lacking IFN signaling specifically in neural tissues. These mice supported a higher acute viral load in nervous tissue and delayed establishment of latency. While latent HSV-1 genome c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.06.016

    authors： Rosato PC,Katzenell S,Pesola JM,North B,Coen DM,Leib DA

    更新日期：2016-10-01 00:00:00

  • An infectious cDNA clone of the poliovirus Sabin strain could be used as a stable repository and inoculum for the oral polio live vaccine.

    abstract：:Viruses were recovered from HeLa S3 cells and African green monkey kidney (AGMK) cells transfected with an infectious cDNA clone of poliovirus vaccine Sabin 1 strain. The viruses recovered from the different DNA-transfected cells were tested for the biological characteristics of temperature sensitivity (rct marker), p...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90100-5

    authors： Kohara M,Abe S,Kuge S,Semler BL,Komatsu T,Arita M,Itoh H,Nomoto A

    更新日期：1986-05-01 00:00:00

  • Sequence and organization of the Mamestra configurata nucleopolyhedrovirus genome.

    abstract：:The nucleotide sequence of the genome of the nucleopolyhedrovirus (NPV) from Mamestra configurata (MacoNPV, isolate 90/2), a group II NPV, was determined and analyzed. The MacoNPV DNA genome consists of 155,060 bp and has an overall G+C content of 41.7%. Computer-assisted analysis predicted 169 open reading frames (OR...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1313

    authors： Li Q,Donly C,Li L,Willis LG,Theilmann DA,Erlandson M

    更新日期：2002-03-01 00:00:00

  • Characterization of the leader papain-like proteinase of MHV-A59: identification of a new in vitro cleavage site.

    abstract：:Sequence analysis of the mouse hepatitis virus, strain A59 (MHV-A59) genome predicts the presence of two papain-like proteinases encoded within the first open reading frame (ORF 1a) of the replicase gene. The more 5' of these domains, the leader papain-like proteinase, is responsible for the cleavage of the amino term...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1281

    authors： Bonilla PJ,Hughes SA,Piñón JD,Weiss SR

    更新日期：1995-06-01 00:00:00

  • Sequences of the N and M genes of the sigma virus of Drosophila and evolutionary comparison.

    abstract：:The genome of the sigma rhabdovirus of Drosophila melanogaster consists of six genes in the order 3' N-2-3-4-G-L 5'. The nucleotide sequences of the N and of the fourth genes were determined from cDNA clones. Each gene contained a single long open reading frame encoding polypeptides with predicted MW of 50 and 25 kDa,...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1177

    authors： Bras F,Teninges D,Dezelee S

    更新日期：1994-04-01 00:00:00

  • Canine parvovirus capsid assembly and differences in mammalian and insect cells.

    abstract：:We examined the assembly processes of the capsid proteins of canine parvovirus (CPV) in mammalian and insect cells. In CPV-infected cells empty capsids assembled within 15 min, and then continued to form over the following 1 h, while full (DNA-containing) capsids were detected only after 60 min, and those accumulated ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0734

    authors： Yuan W,Parrish CR

    更新日期：2001-01-20 00:00:00

  • V3 sequences of paired HIV-1 isolates from blood and cerebrospinal fluid cluster according to host and show variation related to the clinical stage of disease.

    abstract：:The spread of HIV-1 to the nervous system occurs early during infection in a large number of asymptomatic virus carriers. In order to address the question whether the brain is targeted by a group of HIV-1 variants with increased neurotropic properties we have obtained 20 paired isolates from the blood and cerebrospina...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1503

    authors： Keys B,Karis J,Fadeel B,Valentin A,Norkrans G,Hagberg L,Chiodi F

    更新日期：1993-10-01 00:00:00

  • Small amino acid sequence changes within the V2 domain can affect the function of a T-cell line-tropic human immunodeficiency virus type 1 envelope gp120.

    abstract：:Prior studies with recombinant viruses constructed in vitro showed that the V2 domain of envelope gp120, in addition to the required V3 domain, enhances the efficiency of infection of primary macrophages by HIV-1. Present structural studies on the gp120s of these recombinant viruses using three human monoclonal antibo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1010

    authors： Koito A,Stamatatos L,Cheng-Mayer C

    更新日期：1995-02-01 00:00:00

  • The coat protein of turnip crinkle virus is involved in subviral RNA-mediated symptom modulation and accumulation.

    abstract：:Some satellite (sat-) and defective interfering (DI) RNAs associated with plant viruses intensify or ameliorate the symptoms of the virus. We recently demonstrated that the TCV coat protein (CP) is involved in symptom modulation by sat-RNA C. Two additional subviral RNAs have now been tested for effect of the CP on sy...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8853

    authors： Kong Q,Oh JW,Carpenter CD,Simon AE

    更新日期：1997-11-24 00:00:00

  • Domain structure, intracellular trafficking, and beta2-microglobulin binding of a major histocompatibility complex class I homolog encoded by molluscum contagiosum virus.

    abstract：:The MC80R gene of molluscum contagiosum virus (MCV) type 1 encodes a major histocompatibility complex (MHC) class I homolog that lacks several amino-acid residues critical for peptide binding by MHC molecules, contains an unusually long N-terminal hydrophobic domain possibly derived by triplication of a signal peptide...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9390

    authors： Senkevich TG,Moss B

    更新日期：1998-10-25 00:00:00