当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Analysis of African cassava mosaic virus recombinants suggests strand nicking occurs within the conserved nonanucleotide motif during the initiation of rolling circle DNA replication.

Analysis of African cassava mosaic virus recombinants suggests strand nicking occurs within the conserved nonanucleotide motif during the initiation of rolling circle DNA replication.

Abstract：

:Intact clones containing partial repeats of the genomic components of African cassava mosaic (ACMV DNAs A and B) are infectious when mechanically coinoculated onto Nicotiana benthamiana. Monomeric genomic components may be generated either by homologous recombination or, when two copies of the origin of replication (ori) are present, by a modified rolling circle replication mechanism in which nascent single-stranded DNA is resolved by the introduction of nicks at both oris. DNA B partial repeats with duplicated common region sequences containing combinations of wild-type sequences and nonlethal mutations at nucleotides 151 and 155 within the putative stem-loop region have been constructed and introduced into plants in the presence of DNA A. Analysis of progeny indicates that monomers are generated by DNA strand nicking preferentially between nucleotides 151 and 155, suggesting a nonrandom replicative release mechanism involving the ubiquitous TAATATTAC motif (nucleotides 146-154). Viable ACMV DNA A deletion mutants are known to revert to wild-type size during systemic infection by generating tandem repeats. The recombination point in one such revertant has been mapped between nucleotides 152 and 153. Just as ori-nicking enzymes mediate recombinational events during prokaryotic rolling circle DNA replication, the result suggests that a nick has been introduced in the virion-sense strand within the nonanucleotide motif (TAATATT decreases AC) during the initiation of ACMV DNA replication.

journal_name

Virology

journal_title

Virology

authors

Stanley J

doi

10.1016/s0042-6822(95)80093-x

subject

Has Abstract

pub_date

1995-01-10 00:00:00

pages

707-12

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(95)80093-X

journal_volume

206

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Mu-opioid modulation of HIV-1 coreceptor expression and HIV-1 replication.

    abstract：:A substantial proportion of HIV-1-infected individuals are intravenous drug users (i.v.DUs) who abuse opiates. Opioids induce a number of immunomodulatory effects that may directly influence HIV-1 disease progression. In the present report, we have investigated the effect of opioids on the expression of the major HIV-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00015-1

    authors： Steele AD,Henderson EE,Rogers TJ

    更新日期：2003-04-25 00:00:00

  • Production and characterization of monoclonal antibodies to porcine group C rotaviruses cross-reactive with group A rotaviruses.

    abstract：:Five monoclonal antibodies (MAbs) to porcine group (gp) C rotaviruses (Cowden and Ah strains) reactive with both gp A and C rotaviruses in cell culture immunofluorescence (CCIF) tests were produced and characterized. These MAbs reacted with three strains of gp A and two strains of gp C rotaviruses in a CCIF test and w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90189-v

    authors： Tsunemitsu H,Ojeh CK,Jiang B,Simkins RA,Weilnau PA,Saif LJ

    更新日期：1992-11-01 00:00:00

  • Deletion of pe38 attenuates AcMNPV genome replication, budded virus production, and virulence in heliothis virescens.

    abstract：:The pe38 gene product of Autographa californica M nucleopolyhedrovirus (AcMNPV) has been shown to be involved in transcriptionally transactivating viral genes and augmenting viral DNA replication in transient assays. To assess the role of pe38 during infection, we generated a knockout virus, Delta pe38-E9/E9, in which...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00143-0

    authors： Milks ML,Washburn JO,Willis LG,Volkman LE,Theilmann DA

    更新日期：2003-06-05 00:00:00

  • Characteristics of neutralizing antibodies to adenovirus capsid proteins in human and animal sera.

    abstract：:Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutraliz...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.12.014

    authors： Yu B,Dong J,Wang C,Zhan Y,Zhang H,Wu J,Kong W,Yu X

    更新日期：2013-03-15 00:00:00

  • Infection of bovine dendritic cells by rinderpest or measles viruses induces different changes in host transcription.

    abstract：:The morbilliviruses are a closely related genus which are very similar in their sequences and share a common receptor, but nevertheless show significant restriction in the host species in which they cause disease. One contribution to this restriction might be the nature of the hosts' responses to infection. We have us...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.09.031

    authors： Nanda SK,Baron J,Royall E,Robinson L,Falciani F,Baron MD

    更新日期：2009-12-20 00:00:00

  • Antiviral T-cell-independent type 2 antibody responses induced in vivo in the absence of T and NK cells.

    abstract：:Polyomavirus (PyV) infection induces protective T-cell-independent (TI) IgM and IgG responses in T-cell-deficient (TCR beta x delta-/-) mice. In this study, we show that PyV is a TI -2 antigen: B cells with a mutated Bruton's tyrosine kinase (Xid mutants) do not respond to PyV with antibody secretion in the absence of...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0766

    authors： Szomolanyi-Tsuda E,Brien JD,Dorgan JE,Garcea RL,Woodland RT,Welsh RM

    更新日期：2001-02-15 00:00:00

  • Membrane binding properties of EBV gp110 C-terminal domain; evidences for structural transition in the membrane environment.

    abstract：:Gp110 of Epstein-Barr virus (EBV) mainly localizes on nuclear/ER membranes and plays a role in the assembly of EBV nucleocapsid. The C-terminal tail domain (gp110 CTD) is essential for the function of gp110 and the nuclear/ER membranes localization of gp110 is ruled by its C-terminal unique nuclear localization signal...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.06.031

    authors： Park SJ,Seo MD,Lee SK,Lee BJ

    更新日期：2008-09-30 00:00:00

  • Genetic expression in bacteriophage lambda. IV. Effects of P2 prophage on lambda inhibition of host synthesis and lambda gene expression.

    abstract：:The effects of P2 prophage on lambda-mediated depression of host metabolism, and on lambda macromolecular synthesis have been studied. Experiments employing certain lambdabio deletion mutants and lambda-related phages suggest that the same gene products are involved in inhibition of host metabolism (Hin+ function) and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：

    authors： Cohen SN,Chang AC

    更新日期：1971-11-01 00:00:00

  • The P genes of human parainfluenza virus type 1 clinical isolates are polycistronic and microheterogeneous.

    abstract：:The nucleotide sequence of the P gene of human parainfluenza virus type 1 (hPIV1) strain C35 was determined directly from genomic viral RNA and by molecular cloning. The gene contained 1893 nucleotides. Four open reading frames (ORF) capable of encoding a P protein (568 amino acids; M(r) = 64,784), a C' protein (219 a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90712-x

    authors： Power UF,Ryan KW,Portner A

    更新日期：1992-07-01 00:00:00

  • Vaccination with herpes simplex virus type 1 glycoprotein K impairs clearance of virus from the trigeminal ganglia resulting in chronic infection.

    abstract：:Following primary ocular infection, HSV-1 establishes a latent infection in the trigeminal ganglia with the complete absence of detectable infectious virus. Recently, we showed that vaccination of BALB/cJ with HSV-1 glycoprotein K (gK), but not other HSV-1 glycoproteins, significantly exacerbated the severity of HSV-1...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0537

    authors： Ghiasi H,Cai S,Nesburn AB,Wechsler SL

    更新日期：1996-10-01 00:00:00

  • A critical Sp1 element in the rhesus rhadinovirus (RRV) Rta promoter confers high-level activity that correlates with cellular permissivity for viral replication.

    abstract：:KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.10.013

    authors： DeMaster LK,Rose TM

    更新日期：2014-01-05 00:00:00

  • Identification and genomic mapping of the ORF3 and VPg proteins in feline calicivirus virions.

    abstract：:Two minor proteins with molecular masses of 8.5 and 15.5 kDa were identified in feline calicivirus (FCV) virions. Direct sequence analysis showed that the N-terminal sequence of the 8.5-kDa protein was identical to that of the predicted protein encoded by open reading frame 3 (ORF3) of the FCV genome. The N-terminal s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0579

    authors： Sosnovtsev SV,Green KY

    更新日期：2000-11-10 00:00:00

  • Negative regulation of a heterologous promoter by human cytomegalovirus immediate-early protein IE2.

    abstract：:The HCMV IE2 protein promiscuously activates transcription of many viral and cellular genes. IE2 also negatively autoregulates its own expression by binding to a strategically positioned IE2 binding site, called CRS, located immediately downstream of the TATA box of the HCMV major IE promoter. Here we show that IE2 is...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8855

    authors： Tsai HL,Kou GH,Tang FM,Wu CW,Lin YS

    更新日期：1997-11-24 00:00:00

  • Polyomavirus JC infects human brain microvascular endothelial cells independent of serotonin receptor 2A.

    abstract：:Although human polyomavirus JC (JCV) is known to cause progressive multifocal leukoencephalopathy (PML) in immunocompromised individuals, the mechanism by which JCV crosses the blood-brain barrier (BBB) remains unclear. To test our hypothesis that cell-free JCV gains entry into the brain by infecting endothelial cells...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.02.018

    authors： Chapagain ML,Verma S,Mercier F,Yanagihara R,Nerurkar VR

    更新日期：2007-07-20 00:00:00

  • NS-1 and NS-2 proteins may act synergistically in the cytopathogenicity of parvovirus MVMp.

    abstract：:The interaction of parvovirus minute virus of mice (prototype strain, MVMp) with simian virus 40 (SV40)-transformed human cells (NB-E) was investigated by means of transfection with MVMp molecular clones derived from the infectious recombinant plasmid (pMM984). pMM984 inhibits stable transformation of NB-E cells to ge...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90110-d

    authors： Brandenburger A,Legendre D,Avalosse B,Rommelaere J

    更新日期：1990-02-01 00:00:00

  • DNA immunization in combination with effective antiretroviral drug therapy controls viral rebound and prevents simian AIDS after treatment is discontinued.

    abstract：:DNA immunization in conjunction with antiretroviral therapy was evaluated in SIV-infected rhesus macaques treated with [R]-9-[2-phosphonylmethoxypropyl]adenine (PMPA). Macaques were immunized monthly with DNA vaccines expressing either SIV gag/tat or SIV gag/tat and 19 CD8+ T cell epitopes during 7 months of therapy. ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.12.008

    authors： Fuller DH,Rajakumar PA,Wu MS,McMahon CW,Shipley T,Fuller JT,Bazmi A,Trichel AM,Allen TM,Mothe B,Haynes JR,Watkins DI,Murphey-Corb M

    更新日期：2006-04-25 00:00:00

  • Redundancy in the immune system restricts the spread of HSV-1 in the central nervous system (CNS) of C57BL/6 mice.

    abstract：:Resistance to lethal encephalitis in mice infected with HSV-1 via the oral mucosa is mouse strain dependent. In susceptible BALB/c, HSV-1 spreads throughout the CNS but in resistant BL/6 mice, virus is restricted to the brainstem. To examine the contribution of cellular immunity in restricting viral spread, we used a ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.02.013

    authors： Kastrukoff LF,Lau AS,Takei F,Smyth MJ,Jones CM,Clarke SR,Carbone FR

    更新日期：2010-05-10 00:00:00

  • Adenovirus interactions with CD46 on transgenic mouse erythrocytes.

    abstract：:Hemagglutination is an established method but has not been used previously to determine the efficacy of virus binding to a specific cellular receptor. Here we have utilized CD46-expressing erythrocytes from a transgenic mouse to establish whether and to what extent the species B adenoviruses (Ads) as well as Ad37 and ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.03.004

    authors： Andersson EK,Mei YF,Wadell G

    更新日期：2010-06-20 00:00:00

  • The N-terminal part of bacteriophage P2 capsid protein is essential for postassembly maturation of P2 and P4 capsids.

    abstract：:During capsid assembly of bacteriophage P2 and its satellite phage P4, gpN undergoes proteolytic cleavage with the removal of the first 31 amino acids. The truncated protein gpN* is unable to support formation of viable phages in complementation tests. A c-myc antigenic epitope (EQKLISEEDL) exchanged for eleven amino ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0566

    authors： Bjerve K,Lindqvist BH

    更新日期：1996-10-15 00:00:00

  • RNA-binding proteins of coronavirus MHV: detection of monomeric and multimeric N protein with an RNA overlay-protein blot assay.

    abstract：:RNA-binding proteins of coronavirus MHV-A59 were identified using an RNA overlay-protein blot assay (ROPBA). The major viral RNA-binding protein in virions and infected cells was the phosphorylated nucleocapsid protein N (50K). A new 140K virus structural protein was identified as a minor RNA-binding protein both in v...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90305-3

    authors： Robbins SG,Frana MF,McGowan JJ,Boyle JF,Holmes KV

    更新日期：1986-04-30 00:00:00

  • The prolonged culture of human immunodeficiency virus type 1 in primary lymphocytes increases its sensitivity to neutralization by soluble CD4.

    abstract：:Primary strains of human immunodeficiency virus type 1 (HIV-1) are known to adapt to replication in cell lines in vitro by becoming sensitive to soluble CD4 (sCD4) and neutralizing antibodies (NAb). T-cell lines favor isolation of variants that use CXCR4 as a co-receptor, while primary isolates predominantly use CCR5....

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.12.012

    authors： Pugach P,Kuhmann SE,Taylor J,Marozsan AJ,Snyder A,Ketas T,Wolinsky SM,Korber BT,Moore JP

    更新日期：2004-03-30 00:00:00

  • Transcriptional activation of the human immunodeficiency virus type 1 long terminal repeat by hepatitis B virus X-protein requires de novo protein synthesis.

    abstract：:Human hepatitis B virus (HBV) X-gene, previously shown to be capable of trans-activating heterologous regulatory elements of the human beta-interferon gene, the human immunodeficiency virus type I (HIV-1) long terminal repeat (LTR), the simian virus 40 (SV40), and HBV, has the capacity to code for a 17-kDa polypeptide...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90501-h

    authors： Twu JS,Wu JY,Robinson WS

    更新日期：1990-07-01 00:00:00

  • Friend murine leukemia virus A8 regulates Env protein expression through an intron sequence.

    abstract：:Friend murine leukemia virus (Fr-MLV) produces unspliced mRNAs, as well as singly-spliced mRNAs by which the Env protein is generated. We investigated the role of the intron within the Fr-MLV gene in Env expression using vectors with serially truncated introns. The up-regulatory regions, the 361-878 and the 5135-5399 ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.11.040

    authors： Yamamoto N,Takase-Yoden S

    更新日期：2009-03-01 00:00:00

  • Tobacco mosaic virus coat protein and the large subunit of the host protein ribulose-1,5-biphosphate carboxylase share a common antigenic determinant.

    abstract：:An immunological relationship was detected between the coat protein of the common (U1) strain of tobacco mosaic virus (TMV) and the large subunit of the ubiquitous CO2-fixing host enzyme, ribulose-1,5-biphosphate carboxylase (RuBisCo). When assayed by Western immunoblotting or indirect ELISA, polyclonal antisera to TM...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90186-8

    authors： Dietzgen RG,Zaitlin M

    更新日期：1986-11-01 00:00:00

  • Tick-borne flavivirus infection in Ixodes scapularis larvae: development of a novel method for synchronous viral infection of ticks.

    abstract：:Following a bite from an infected tick, tick-borne flaviviruses cause encephalitis, meningitis and hemorrhagic fever in humans. Although these viruses spend most of their time in the tick, little is known regarding the virus-vector interactions. We developed a simple method for synchronously infecting Ixodes scapulari...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.03.057

    authors： Mitzel DN,Wolfinbarger JB,Long RD,Masnick M,Best SM,Bloom ME

    更新日期：2007-09-01 00:00:00

  • Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome.

    abstract：:We have recently described the cloning of a portion of the hepatitis E virus (HEV) and confirmed its etiologic association with enterically transmitted (waterborne, epidemic) non-A, non-B hepatitis. The virus consists of a single-stranded, positive-sense RNA genome of approximately 7.5 kb, with a polyadenylated 3' end...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90760-9

    authors： Tam AW,Smith MM,Guerra ME,Huang CC,Bradley DW,Fry KE,Reyes GR

    更新日期：1991-11-01 00:00:00

  • Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin.

    abstract：:The binding of influenza virus to erythrocytes and host cells is mediated by the interaction of the viral hemagglutinin (H) with cell surface receptors containing sialic acid (SA). The specificity of this interaction for 19 human and animal influenza isolates was examined using human erythrocytes enzymatically modifie...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90150-2

    authors： Rogers GN,Paulson JC

    更新日期：1983-06-01 00:00:00

  • Highly complex neutralization determinants on a monophyletic lineage of newly transmitted subtype C HIV-1 Env clones from India.

    abstract：:Little is known about the neutralization properties of HIV-1 in India to optimally design and test vaccines. For this reason, a functional Env clone was obtained from each of ten newly acquired, heterosexually transmitted HIV-1 infections in Pune, Maharashtra. These clones formed a phylogenetically distinct genetic li...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.12.032

    authors： Kulkarni SS,Lapedes A,Tang H,Gnanakaran S,Daniels MG,Zhang M,Bhattacharya T,Li M,Polonis VR,McCutchan FE,Morris L,Ellenberger D,Butera ST,Bollinger RC,Korber BT,Paranjape RS,Montefiori DC

    更新日期：2009-03-15 00:00:00

  • An analysis of the requirements for human cytomegalovirus oriLyt-dependent DNA synthesis in the presence of the herpes simplex virus type 1 replication fork proteins.

    abstract：:Activation of the human cytomegalovirus (HCMV) origin of replication (oriLyt) was previously demonstrated in transient transfection assays in permissive human fetal fibroblasts and nonpermissive Vero cells, and shown to require six viral proteins that function at the replication fork plus a number of HCMV products tha...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00005-9

    authors： Reid GG,Ellsmore V,Stow ND

    更新日期：2003-04-10 00:00:00

  • Transport of a lysosomally targeted Rous sarcoma virus envelope glycoprotein involves transient expression on the cell surface.

    abstract：:The details of intracellular transport pathways for glycosylated proteins remain incompletely described. We previously described a mutant Rous sarcoma virus envelope glycoprotein (gp), mu 26, with an altered membrane-spanning domain that was targeted to lysosomes after traversing the trans-Golgi. This mutant protein w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(95)80050-6

    authors： Johnston PB,Dong JY,Hunter E

    更新日期：1995-01-10 00:00:00