当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > RNA-binding proteins of coronavirus MHV: detection of monomeric and multimeric N protein with an RNA overlay-protein blot assay.

RNA-binding proteins of coronavirus MHV: detection of monomeric and multimeric N protein with an RNA overlay-protein blot assay.

Abstract：

:RNA-binding proteins of coronavirus MHV-A59 were identified using an RNA overlay-protein blot assay (ROPBA). The major viral RNA-binding protein in virions and infected cells was the phosphorylated nucleocapsid protein N (50K). A new 140K virus structural protein was identified as a minor RNA-binding protein both in virions and in infected cells. The 140K protein was antigenically related to N, and upon reduction, yielded only 50K N. Thus, the 140K protein is probably a trimer of N subunits linked by intermolecular disulfide bonds. Several cellular RNA-binding proteins were also detected. RNA-binding of N was not nucleotide sequence specific. Single-stranded RNA of MHV, VSV, or cellular origin, a DNA probe of the MHV leader sequence, and double-stranded bovine rotavirus RNA could all bind to N. Binding of MHV RNA was optimal between pH 7 and 8, and the RNA could be eluted in 0.1 M NaCl. The ROPBA is a useful method for the initial identification of RNA-binding proteins, such as N and the 140K protein of murine coronavirus.

journal_name

Virology

journal_title

Virology

authors

Robbins SG,Frana MF,McGowan JJ,Boyle JF,Holmes KV

doi

10.1016/0042-6822(86)90305-3

subject

Has Abstract

pub_date

1986-04-30 00:00:00

pages

402-10

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

150

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • E1B and E4 oncoproteins of adenovirus antagonize the effect of apoptosis inducing factor.

    abstract：:Adenovirus inundates the productively infected cell with linear, double-stranded DNA and an abundance of single-stranded DNA. The cellular response to this stimulus is antagonized by the adenoviral E1B and E4 early genes. A mutant group C adenovirus that fails to express the E1B-55K and E4orf3 genes is unable to suppr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.03.010

    authors： Turner RL,Wilkinson JC,Ornelles DA

    更新日期：2014-05-01 00:00:00

  • Assessment and optimization of rolling circle amplification protocols for the detection and characterization of badnaviruses.

    abstract：:The genus Badnavirus is characterized by members that are genetically and serologically heterogeneous which presents challenges for their detection and characterization. The presence of integrated badnavirus-like sequences in some host species further complicates detection using PCR-based protocols. To address these c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.01.013

    authors： Sukal AC,Kidanemariam DB,Dale JL,Harding RM,James AP

    更新日期：2019-03-01 00:00:00

  • Acquisition of a novel eleven amino acid insertion directly N-terminal to a tetrabasic cleavage site confers intracellular cleavage of an H7N7 influenza virus hemagglutinin.

    abstract：:A critical feature of highly pathogenic avian influenza viruses (H5N1 and H7N7) is the efficient intracellular cleavage of the hemagglutinin (HA) protein. H7N7 viruses also exist in equine species, and a unique feature of the equine H7N7 HA is the presence of an eleven amino acid insertion directly N-terminal to a tet...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.09.004

    authors： Hamilton BS,Sun X,Chung C,Whittaker GR

    更新日期：2012-12-05 00:00:00

  • Reactivities of human sera with human herpesvirus-8-infected BCBL-1 cells and identification of HHV-8-specific proteins and glycoproteins and the encoding cDNAs.

    abstract：:The reactivates of human sera with uninduced and phorbol ester (TPA)-induced human herpesvirus-8 (HHV-8)-infected BCBL-1 cells were examined by immunofluorescence assay (IFA) and by radioimmunoprecipitation reactions (RIP). The seroprevalence of HHV-8 infections is low in the United States general population and only ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9055

    authors： Chandran B,Smith MS,Koelle DM,Corey L,Horvat R,Goldstein E

    更新日期：1998-03-30 00:00:00

  • Analysis of the nucleotide sequence of bean pod mottle virus middle component RNA.

    abstract：:The complete nucleotide sequence of the middle component RNA (M RNA) of the comovirus bean pod mottle virus (BPMV) has been determined. The sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 Da. The proteolytic processing sites within this pr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90155-5

    authors： MacFarlane SA,Shanks M,Davies JW,Zlotnick A,Lomonossoff GP

    更新日期：1991-07-01 00:00:00

  • Bacteriophage P22 capsid size determination: roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus.

    abstract：:Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.06.025

    authors： Suhanovsky MM,Teschke CM

    更新日期：2011-09-01 00:00:00

  • Effects of mutations in the C-terminal region of NIa protease on cis-cleavage between NIa and NIb.

    abstract：:Mutational analyses were carried out to investigate whether the nuclear inclusion protein a (NIa) C-terminal amino acids of turnip mosaic potyvirus play any roles in the cis-cleavage between NIa and NIb. The processing rate of the NIa-NIb junction sequence was decreased significantly by either V240D or Q243A mutation ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8938

    authors： Kim DH,Han JS,Lew J,Kim SS,Kang BH,Hwang DC,Jang DS,Kim W,Song BD,Choi KY

    更新日期：1998-02-01 00:00:00

  • Two adenovirus proteins with redundant activities in virus growth facilitates tripartite leader mRNA accumulation.

    abstract：:Most adenovirus-specific mRNAs expressed late after infection originate from a single transcription unit, the so-called major late transcription unit. All mRNAs expressed from this unit have in common a 201 nucleotide-long spliced tripartite leader segment attached to their 5' ends. Human adenovirus mutants that carry...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1234

    authors： Ohman K,Nordqvist K,Akusjärvi G

    更新日期：1993-05-01 00:00:00

  • Antibodies targeting dengue virus envelope domain III are not required for serotype-specific protection or prevention of enhancement in vivo.

    abstract：:The envelope (E) protein of dengue virus (DENV) is composed of three domains (EDI, EDII, EDIII) and is the main target of neutralizing antibodies. Many monoclonal antibodies that bind EDIII strongly neutralize DENV. However in vitro studies indicate that anti-EDIII antibodies contribute little to the neutralizing pote...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.03.003

    authors： Williams KL,Wahala WM,Orozco S,de Silva AM,Harris E

    更新日期：2012-07-20 00:00:00

  • Human herpesvirus type 8 variants circulating in Europe, Africa and North America in classic, endemic and epidemic Kaposi's sarcoma lesions during pre-AIDS and AIDS era.

    abstract：:Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.12.005

    authors： Tornesello ML,Biryahwaho B,Downing R,Hatzakis A,Alessi E,Cusini M,Ruocco V,Katongole-Mbidde E,Loquercio G,Buonaguro L,Buonaguro FM

    更新日期：2010-03-15 00:00:00

  • Biological characterization of infectious molecular clones derived from a human immunodeficiency virus type-1 isolate with rapid/high replicative capacity.

    abstract：:In order to molecularly characterize rapidly and slowly replicating HIV-1 variants, molecular clones were obtained from a rapid/high virus isolate. This isolate, 4803, had only been passaged in peripheral blood mononuclear cells (PBMC) prior to cloning. Molecular cloning was done in bacteriophage lambda-dash using hig...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90469-r

    authors： Fredriksson R,Stålhanske P,von Gegerfelt A,Lind B,Aman P,Rassart E,Fenyö EM

    更新日期：1991-03-01 00:00:00

  • Systematic search fails to detect immunogenic MHC class-I-restricted determinants encoded by influenza A virus noncoding sequences.

    abstract：:It has been demonstrated in a number of systems that CD8+ T cells (T CD8+) can be induced by peptides encoded in alternative reading frames (ARFs) that do not appear to code for bona fide proteins. The biological relevance of ARF peptides remains to be firmly established, however. With this as a goal, we systematicall...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1744

    authors： Chen W,Bennink JR,Yewdell JW

    更新日期：2003-01-05 00:00:00

  • Ligation of double-stranded and single-stranded [oligo(dT)] DNA by vaccinia virus DNA ligase.

    abstract：:Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed t...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0358

    authors： Odell M,Kerr SM,Smith GL

    更新日期：1996-07-01 00:00:00

  • Association of polymorphisms in NFκB1 promoter and NFκBIA gene with the development of antibodies against HHV-8 in HIV-infected individuals.

    abstract：:Human gammaherpesvirus 8 (HHV-8) is the etiologic agent of Kaposi's sarcoma, multicentric Castleman's disease and primary effusion lymphoma. Like other herpesviruses, the HHV-8 may exhibit latent or lytic cycle, both regulated by viral and host factors. Regarding host factors, we analysed the association of polymorphi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.07.011

    authors： Gonçales JP,Silva Júnior JVJ,Lopes TRR,Tozetto-Mendoza TR,de Farias Guimarães D,de Morais VMS,Coêlho MRCD

    更新日期：2019-09-01 00:00:00

  • Preliminary in vitro growth cycle and transmission studies of HIV-1 in an autologous primary cell assay of blood-derived macrophages and peripheral blood mononuclear cells.

    abstract：:Recent interest focused on the dynamics of HIV-1 replication in primary monocytes/macrophages and T-lymphocytes of the immune system, as well as the standardization of virological and immunological in vitro assays with primary isolates, provided the impetus for these studies. These types of studies have never been per...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0648

    authors： Tsai WP,Conley SR,Kung HF,Garrity RR,Nara PL

    更新日期：1996-12-15 00:00:00

  • An orthologue of the cor gene is involved in the exclusion of temperate lambdoid phages. Evidence that Cor inactivates FhuA receptor functions.

    abstract：:A new set of lambdoid phages (mEp) classified into different immunity groups was previously described. Phages mEp213, mEp237, and mEp410 were unable to grow in mEp167 lysogenic cells, presumably due to an exclusion mechanism expressed constitutively by the mEp167 repressed prophage. In this work, to analyze the exclus...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.09.005

    authors： Uc-Mass A,Loeza EJ,de la Garza M,Guarneros G,Hernández-Sánchez J,Kameyama L

    更新日期：2004-11-24 00:00:00

  • Complementation analysis of the dales collection of vaccinia virus temperature-sensitive mutants.

    abstract：:A collection of randomly generated temperature-sensitive (ts) vaccinia virus (strain IHD-W) mutants were reported by S. Dales et al., (1978, Virology, 84, 403-428) in 1978 and characterized by electron microscopy. We have performed further genetic analysis on the Dales collection of mutants to make the mutants more us...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1745

    authors： Lackner CA,D'Costa SM,Buck C,Condit RC

    更新日期：2003-01-20 00:00:00

  • Betanodavirus induces phosphatidylserine exposure and loss of mitochondrial membrane potential in secondary necrotic cells, both of which are blocked by bongkrekic acid.

    abstract：:In this study, we show how the red spotted grouper nervous necrosis virus (RGNNV) causes loss of mitochondrial membrane potential and promotes host secondary apoptotic necrosis. RGNNV viral proteins such as protein alpha (42 kDa) and protein A (110 kDa) were quickly expressed between 12 h and 24 h postinfection (p.i.)...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.11.052

    authors： Chen SP,Yang HL,Her GM,Lin HY,Jeng MF,Wu JL,Hong JR

    更新日期：2006-04-10 00:00:00

  • Simian virus 40 T antigen is detected only in cells in the G2 phase of the cell cycle in one group of rat transformants.

    abstract：:The relationship between the cell cycle and the presence of the nuclear T antigen in simian virus 40-transformed rat cells independently derived from FR 3T3 cells has been investigated using a fluorescence-activated cell sorter and a combination of stainings for cellular DNA (4',6-diamidine-2-phenylindole dihydrochlor...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(83)80028-2

    authors： Imbert J,Lawrence JJ,Birg F

    更新日期：1983-04-30 00:00:00

  • Polymorphisms of the cytidine deaminase APOBEC3F have different HIV-1 restriction efficiencies.

    abstract：:The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.11.004

    authors： Mohammadzadeh N,Follack TB,Love RP,Stewart K,Sanche S,Chelico L

    更新日期：2019-01-15 00:00:00

  • Physical characterization of the genome of feline herpesvirus-1.

    abstract：:The physical structure of the genome of feline herpesvirus-1, a major upper respiratory tract pathogen of cats, was studied. Purified FHV-1 DNA was analyzed by restriction endonuclease and gamma 5' exonuclease digestion, blot hybridization, and electron microscopy. To facilitate further studies, nine bacteriophage clo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90439-3

    authors： Rota PA,Maes RK,Ruyechan WT

    更新日期：1986-10-15 00:00:00

  • The sequence of adenovirus fiber: similarities and differences between serotypes 2 and 5.

    abstract：:The amino acid sequence of the fiber from adenovirus type 5 has been deduced from the nucleotide sequence of the fiber gene. This sequence is compared with that of adenovirus type 2, a closely related serotype. We find 69% homology for the fiber protein whereas the known nonstructural proteins of these two serotypes h...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90150-4

    authors： Chroboczek J,Jacrot B

    更新日期：1987-12-01 00:00:00

  • Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.

    abstract：:The potential role(s) of cytokines in the reduction of infectious virus and persistent viral infection in the central nervous system was examined by determining the kinetics of cytokine mRNA expression following infection with the neurotropic JHM strain of mouse hepatitis virus. Mice were infected with an antibody esc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8613

    authors： Parra B,Hinton DR,Lin MT,Cua DJ,Stohlman SA

    更新日期：1997-07-07 00:00:00

  • Analysis of the retrovirus capsid interdomain linker region.

    abstract：:In structural studies, the retrovirus capsid interdomain linker region has been shown as a flexible connector between the CA N-terminal domain and its C-terminal domain. To analyze the function of the linker region, we have examined the effects of three Moloney murine leukemia virus (M-MuLV) capsid linker mutations/va...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(02)00142-3

    authors： Arvidson B,Seeds J,Webb M,Finlay L,Barklis E

    更新日期：2003-03-30 00:00:00

  • Epitopes of the G1 glycoprotein of La Crosse virus form overlapping clusters within a single antigenic site.

    abstract：:Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90283-1

    authors： Najjar JA,Gentsch JR,Nathanson N,Gonzalez-Scarano F

    更新日期：1985-07-30 00:00:00

  • Abolition of hCAR-dependent cell tropism using fiber knobs of Atadenovirus serotypes.

    abstract：:Most adenoviral vectors use in gene therapy protocols derive from species C. However, expression of the primary receptor (human Coxsackievirus and Adenovirus receptor, hCAR) for these AdV is variable on cancer cells. In vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therap...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.12.023

    authors： Renaut L,Colin M,Leite JP,Benko M,D'Halluin JC

    更新日期：2004-04-10 00:00:00

  • Tumorigenicity of adenovirus-transformed cells: region E1A of adenovirus 12 confers resistance to natural killer cells.

    abstract：:Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12E1AB virus (containing E1A and E1B...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90143-6

    authors： Sawada Y,Föhring B,Shenk TE,Raska K Jr

    更新日期：1985-12-01 00:00:00

  • Pirates of the Caudovirales.

    abstract：:Molecular piracy is a biological phenomenon in which one replicon (the pirate) uses the structural proteins encoded by another replicon (the helper) to package its own genome and thus allow its propagation and spread. Such piracy is dependent on a complex web of interactions between the helper and the pirate that occu...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2012.10.028

    authors： Christie GE,Dokland T

    更新日期：2012-12-20 00:00:00

  • Cell-specific kinetics and efficiency of herpes simplex virus type 1 entry are determined by two distinct phases of attachment.

    abstract：:We previously provided evidence for a model of herpes simplex virus type 1 (HSV-1) entry by a cascade of interactions between components of the virion envelope and cellular plasma membrane (Fuller and Lee, 1992, J. Virol. 66, 5002-5012). In this report we have determined entry kinetics of wild-type HSV-1 into two high...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1081

    authors： McClain DS,Fuller AO

    更新日期：1994-02-01 00:00:00

  • Mutation and replacement of the 16-kDa protein gene in RNA-1 of tobacco rattle virus.

    abstract：:The function of the 16-kDa protein encoded by tobacco rattle virus (TRV) RNA-1 was investigated by a mutational analysis of the 16-kDa protein gene. Transcripts of TRV RNA-1 produced from a full-length cDNA clone of TRV RNA-1 (SYM strain) remained infectious when the 16-kDa protein gene was disrupted by premature term...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90601-7

    authors： Guilford PJ,Ziegler-Graff V,Baulcombe DC

    更新日期：1991-06-01 00:00:00