Abstract:
:Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype of F170A and F170K coat protein mutants were isolated. Suppressors were repeatedly found in the coat protein telokin-like domain at position 285, which caused coat protein to assemble into petite procapsids and capsids. Petite capsid assembly strongly correlated to the side chain volume of the substituted amino acid. We hypothesize that larger side chains at position 285 torque the telokin-like domain, changing flexibility of the subunit and intercapsomer contacts. Thus, a single amino acid substitution in coat protein is sufficient to change capsid size. In addition, the products of assembly of the variant coat proteins were affected by the size of the internal scaffolding protein.
journal_name
Virologyjournal_title
Virologyauthors
Suhanovsky MM,Teschke CMdoi
10.1016/j.virol.2011.06.025subject
Has Abstractpub_date
2011-09-01 00:00:00pages
418-29issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(11)00289-3journal_volume
417pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The major capsid protein of HPV, L1, assembles into pentamers that form a T = 7 icosahedral particle, but the location of the co-assembled minor capsid protein, L2, remains controversial. Several researchers have developed useful monoclonal antibodies targeting L2, but most react with linear epitopes toward the N-term...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.08.017
更新日期:2018-11-01 00:00:00
abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0002
更新日期:1996-01-01 00:00:00
abstract::From previously constructed genomic libraries of a Taiwan WSSV isolate, a putative WSSV tk-tmk gene was identified. Uniquely, the open reading frame (ORF) of this gene was predicted to encode a novel chimeric protein of 388 amino acids with significant homology to two proteins: thymidine kinase (TK) and thymidylate ki...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0597
更新日期:2000-11-10 00:00:00
abstract::Ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB (MoMuLV-TB), causes a progressive hindlimb paralytic disease in susceptible strains of mice. Previously, it has been shown that a single amino acid substitution, Val-25----Ile in gPr80env, is responsible for the temperature sensitivity, inefficien...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90438-h
更新日期:1991-10-01 00:00:00
abstract::We have characterized the fibergenes of adenovirus type 19p (Ad19p), Ad19a, and Ad37 by sequencing. The fiber genes of Ad19a and Ad37 are identical and only five amino acids differ comparing Ad19a/Ad37 with Ad19p. Based on the translated sequences we calculated the isoelectrical points (Ips) and found that the fiber k...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8269
更新日期:1997-01-06 00:00:00
abstract::Torque Teno Virus (TTV) has been assigned to the floating genus Anellovirus. TTV ssDNA genomes have a size of 3.6 to 3.8 kb and display up to 30% nucleotide diversity. The pathogenic potential of TTV is under investigation. To address a putative link of pathogenicity with the observed sequence variations, the transcri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.017
更新日期:2008-11-10 00:00:00
abstract::The replication of vaccinia virus was reduced by 3 logs in cells that had been treated before and during infection with a concentration of N-(phosphonoacetyl)-L-aspartate (PALA) which lowered the UTP and CTP to 5 and 20% of controls, respectively, without affecting cell viability. The antiviral activity of PALA was re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8735
更新日期:1997-09-15 00:00:00
abstract::EBNA-1 is the sole virally specified protein required for replication of the Epstein-Barr virus latency origin and also modulates its own expression. Both of these functions are mediated through specific DNA binding. We created a series of amino acid substitutions across the previously identified DNA-binding domain of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1669
更新日期:1994-12-01 00:00:00
abstract::New sequences have been obtained by successive overlapping RT-PCR extensions from the pol region of a retroviral RNA (multiple sclerosis-associated retroviral element, MSRV) amplified in retrovirus-like particles from patients with multiple sclerosis. gag and pol sequences are related to type C oncoviruses, whereas th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9792
更新日期:1999-07-20 00:00:00
abstract::The alphaherpesviruses establish latent infections in sensory neuronal cells, during which a number of latency-associated RNA transcripts (LATs) are produced. The reasons for the production of the LATs, however, and the mechanism by which the LAT region of the genome remains active during latency are unknown. Here we ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1267
更新日期:1995-06-01 00:00:00
abstract::Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain E...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.03.013
更新日期:2017-06-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) infection induces changes to the host cell nucleus including relocalization of the cellular protein Upstream Binding Factor (UBF) from the nucleolus to viral replication compartments (VRCs). Herein, we tested the hypothesis that UBF is recruited to VRCs to promote viral DNA replication. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.04.003
更新日期:2015-09-01 00:00:00
abstract::Adsorption and one-step growth experiments performed with blue-green algal virus (BGAV) LPP-1 under normal conditions revealed a slow adsorption rate, in comparison with bacteriophage systems, followed by a 7-hour latent period. The burst size was approximately 100 virus particles per cell. The molecular weight of the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90036-0
更新日期:1967-08-01 00:00:00
abstract::The papillomavirus (PV) E2 protein activates transcription and replication by recruiting cellular proteins and the E1 DNA helicase to their binding sites in the viral genome. We recently demonstrated that phosphorylation of tyrosine 102 in the bovine papillomavirus (BPV-1) E2 protein restricts these activities and tha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.05.013
更新日期:2018-08-01 00:00:00
abstract::Guinea pig cytomegalovirus (GPCMV) immediate early proteins, IE1 and IE2, demonstrated structural and functional homologies with human cytomegalovirus (HCMV). GPCMV IE1 and IE2 co-localized in the nucleus with each other, the viral polymerase and guinea pig ND10 components (gpPML, gpDaxx, gpSp100, gpATRX). IE1 showed ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.01.023
更新日期:2017-04-01 00:00:00
abstract::We examined the native plant host Solanum nigrum as reservoir of genetic diversity of begomoviruses that cause the tomato yellow leaf curl disease (TYLCD) emerging in southern Spain. Presence of isolates of all the species and strains found associated with TYLCD in this area was demonstrated. Mixed infections were com...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.02.028
更新日期:2006-07-05 00:00:00
abstract::In mid-January 2016, an outbreak of H7N8 high-pathogenicity avian influenza virus (HPAIV) in commercial turkeys occurred in Indiana. Surveillance within the 10km control zone identified H7N8 low-pathogenicity avian influenza virus (LPAIV) in nine surrounding turkey flocks but no other HPAIV-affected premises. We seque...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.04.025
更新日期:2017-07-01 00:00:00
abstract::Cells transformed with specific regions of the foot-and-mouth disease virus (FMDV) genome have been constructed and analyzed with respect to viability and susceptibility to FMDV infection. Constitutive expression of an active protease 3C under the control of the tk promoter has been documented by the ability of transf...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1459
更新日期:1995-09-10 00:00:00
abstract::Alternations of cellular gene expression following an adenovirus type 2 infection of human primary cells were studied by using superior sensitive cDNA sequencing. In total, 3791 cellular genes were identified as differentially expressed more than 2-fold. Genes involved in DNA replication, RNA transcription and cell cy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.12.006
更新日期:2012-03-15 00:00:00
abstract::HIV-1 infection is characterized by life-long viral persistence and continued decline of helper CD4 T cells. The new generation of humanized mouse models that encompass RAG-hu, hNOG and BLT mice have been shown to be susceptible to HIV-1 infection and display CD4 T cell loss. Productive infection has been demonstrated...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.10.034
更新日期:2010-02-05 00:00:00
abstract::It is well established that the human subgroup C adenovirus type 5 (Ad5) E1B 55 kDa protein can regulate the activity and concentration of the cellular tumor suppressor, p53. However, the contribution(s) of these functions of the E1B protein to viral reproduction remains unclear. To investigate this issue, we examined...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.016
更新日期:2008-09-01 00:00:00
abstract::Following a bite from an infected tick, tick-borne flaviviruses cause encephalitis, meningitis and hemorrhagic fever in humans. Although these viruses spend most of their time in the tick, little is known regarding the virus-vector interactions. We developed a simple method for synchronously infecting Ixodes scapulari...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.057
更新日期:2007-09-01 00:00:00
abstract::Nipah virus (NiV) causes severe encephalitis in humans, with high mortality. NiV nonstructural C protein (NiV-C) is essential for its pathogenicity, but its functions are unclear. In this study, we focused on NiV-C trafficking in cells and found that it localizes predominantly in the cytoplasm but partly in the nucleu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.013
更新日期:2016-10-01 00:00:00
abstract::The Epstein-Barr virus open reading frame BLLF1 encodes the major envelope glycoproteins gp350 and gp220. Fragments of the gp350/220 gene were expressed in Escherichia coli in order to define regions of the polypeptide chain reacting with human sera. The C-terminal half of the protein was sufficient for recognition by...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90207-6
更新日期:1992-11-01 00:00:00
abstract::We have recently described the cloning of a portion of the hepatitis E virus (HEV) and confirmed its etiologic association with enterically transmitted (waterborne, epidemic) non-A, non-B hepatitis. The virus consists of a single-stranded, positive-sense RNA genome of approximately 7.5 kb, with a polyadenylated 3' end...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90760-9
更新日期:1991-11-01 00:00:00
abstract::In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1058
更新日期:1994-02-01 00:00:00
abstract::We have previously shown that peroxisome proliferator-activated receptor-gamma (PPARgamma) agonists inhibited the inflammatory response of RSV-infected human lung epithelial cells. In this study, we supply evidence that specific PPARgamma agonists (15d-PGJ2, ciglitazone, troglitazone, Fmoc-Leu) efficiently blocked the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.03.008
更新日期:2006-07-05 00:00:00
abstract::Retroperitoneal fibromatosis herpesvirus (RFHV), the macaque homolog of the human rhadinovirus, Kaposi's sarcoma-associated herpesvirus (KSHV), was first identified in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS. We cloned and sequenced the ORF73 latency-associated nuclear antigen (LAN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.022
更新日期:2006-10-10 00:00:00
abstract::Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90149-8
更新日期:1987-12-01 00:00:00
abstract::Magnetic birefringence measurements are reported on solutions of two double-stranded DNA containing viruses: icosahedral cauliflower mosaic virus (CaMV) and SD, an isometric bacteriophage with a short tail. Assuming the signal comes predominantly from the DNA, constraints can be put on its packing geometry. The anisot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90232-1
更新日期:1986-12-01 00:00:00