Abstract:
:The Epstein-Barr virus open reading frame BLLF1 encodes the major envelope glycoproteins gp350 and gp220. Fragments of the gp350/220 gene were expressed in Escherichia coli in order to define regions of the polypeptide chain reacting with human sera. The C-terminal half of the protein was sufficient for recognition by all VCA-positive sera tested. A membrane anchor truncated version of gp350/220 was expressed in insect cells using the baculovirus system. Proteins of different sizes were specifically detected in the cells while a glycosylated 220-kDa protein was secreted. The insect cells were tested for their suitability as tools for performing monospecific immunofluorescence.
journal_name
Virologyjournal_title
Virologyauthors
Nuebling CM,Buck M,Boos H,von Deimling A,Mueller-Lantzsch Ndoi
10.1016/0042-6822(92)90207-6subject
Has Abstractpub_date
1992-11-01 00:00:00pages
443-7issue
1eissn
0042-6822issn
1096-0341journal_volume
191pub_type
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