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Cellular minichromosome maintenance complex component 5 (MCM5) is incorporated into HIV-1 virions and modulates viral replication in the newly infected cells.

Abstract：

:The post-entry events of HIV-1 infection occur within reverse transcription complexes derived from the viral cores entering the target cell. HIV-1 cores contain host proteins incorporated from virus-producing cells. In this report, we show that MCM5, a subunit of the hexameric minichromosome maintenance (MCM) DNA helicase complex, associates with Gag polyprotein and is incorporated into HIV-1 virions. The progeny virions depleted of MCM5 demonstrated reduced reverse transcription in newly infected cells, but integration and subsequent replication steps were not affected. Interestingly, increased packaging of MCM5 into the virions also led to reduced reverse transcription, but here viral replication was impaired. Our data suggest that incorporation of physiological amounts of MCM5 promotes aberrant reverse transcription, leading to partial incapacitation of cDNA, whereas increased MCM5 abundance leads to reduced reverse transcription and infection. Therefore, MCM5 has the properties of an inhibitory factor that interferes with production of an integration-competent cDNA product.

journal_name

Virology

journal_title

Virology

authors

Santos S,Obukhov Y,Nekhai S,Pushkarsky T,Brichacek B,Bukrinsky M,Iordanskiy S

doi

10.1016/j.virol.2016.06.023

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

11-22

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(16)30164-7

journal_volume

497

pub_type

杂志文章

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