Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.

abstract：:The results of a previous study suggested that the poly(A) sequence in barley stripe mosaic virus (BSMV) RNA is intercalated between a 3'-terminal tyrosine-accepting structure and the 5'-terminal coding part of the BSMV genome. Here we show that poly(A)+ and poly(A)- fractions of BSMV RNA can be cleaved into two fragm...

journal_title：Virology

authors： Agranovsky AA,Dolja VV,Atabekov JG

更新日期：1982-05-01 00:00:00

abstract：:Hepatitis C virus (HCV) core protein is a multifunctional protein interacting with cellular and viral proteins and promoters. A tetracycline-regulated system was used to generate a HepG2 Tet-Off cell line allowing regulated expression of a full-length (191 aa) and an N(c)-truncated core protein (160 aa). In this syste...

journal_title：Virology

authors： Erhardt A,Hassan M,Heintges T,Häussinger D

更新日期：2002-01-20 00:00:00

abstract：:Matrix protein M1 purified from influenza A and B viruses has been analyzed for its ability to specifically interact with cellular proteins by immune coprecipitation and by an in vitro binding assay on nitrocellulose on PVDF membranes. When M1 was mixed with lysates of uninfected cells there was selective binding of h...

journal_title：Virology

authors： Zhirnov OP,Klenk HD

更新日期：1997-09-01 00:00:00

abstract：:The 3' ends of three genomic RNAs (gRNAs) of cowpea chlorotic mottle virus (CCMV) terminate in a highly conserved tRNA-like structure (3'TLS). To examine the intrinsic role played the 3'TLS in packaging, the competence of each gRNA lacking the 3' TLS (DeltaTLS-gRNA) to interact with dissociated coat protein (CP) subun...

journal_title：Virology

authors： Annamalai P,Rao AL

更新日期：2005-02-20 00:00:00

abstract：:Murine gammaherpesvirus 68 (MHV-68) replicates robustly in cell culture, providing a model for studying viral genome replication during de novo infection of tumor-associated herpesviruses. We have previously identified a 1.25-kb origin of lytic replication (oriLyt) for MHV-68. To further investigate the molecular mech...

journal_title：Virology

authors： Gong D,Qi J,Arumugaswami V,Sun R,Deng H

更新日期：2009-05-10 00:00:00

abstract：:Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral ge...

journal_title：Virology

authors： Mizutani T,Sayama Y,Nakanishi A,Ochiai H,Sakai K,Wakabayashi K,Tanaka N,Miura E,Oba M,Kurane I,Saijo M,Morikawa S,Ono S

更新日期：2011-03-30 00:00:00

abstract：:Marek' disease virus serotype-1, also know as Gallid herpesvirus 2 (GaHV-2), elicits T-cell lymphomas in chickens. The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that Meq homodimers are not sufficient to induce lymphomas in chickens. In this s...

journal_title：Virology

authors： Suchodolski PF,Izumiya Y,Lupiani B,Ajithdoss DK,Lee LF,Kung HJ,Reddy SM

更新日期：2010-04-10 00:00:00

abstract：:By employing two different buffer solutions for polyacrylamide gel electrophoresis, all genome segments of Fiji disease and maize rough dwarf virus were separated. Fiji disease virus contains ten genome segments with approximate genome molecular weights of 19.26 x 10(6) and 19.85 x 10(6), depending on the buffer emplo...

journal_title：Virology

authors： Reddy DV,Boccardo G,Outridge R,Teakle DS,Black LM

更新日期：1975-01-01 00:00:00

abstract：:A subtype of human papillomavirus 5 (HPV-5b) is closely associated with carcinomas in the disease epidermodysplasia verruciformis (EV). The complete genome was cloned from virus particles in benign lesions of a patient with EV and sequenced: it was 7779 nucleotides long and consisted of six open reading frames (ORFs) ...

journal_title：Virology

authors： Yabe Y,Sakai A,Hitsumoto T,Kato H,Ogura H

更新日期：1991-08-01 00:00:00

abstract：:Attachment of human adenoviruses (Ads) to host cells is mediated by the interaction of the fiber protein of the capsid with specific cell-surface molecules. For one of the species B adenoviruses, Ad3, the mechanism of binding to cells remains to be defined. Several previous reports have proposed CD46, CD80 or CD86 as ...

journal_title：Virology

authors： Hall K,Blair Zajdel ME,Blair GE

更新日期：2009-09-30 00:00:00

abstract：:The spike (S) and hemagglutinin/esterase (HE) of bovine coronavirus (BCV) are the two envelope proteins that recognize the same receptor-determinant of 9-O-acetylneuraminic acid on host cells. However, the precise and relative roles of the two proteins in BCV infectivity remain elusive. To unequivocally determine thei...

journal_title：Virology

authors： Popova R,Zhang X

更新日期：2002-03-01 00:00:00

abstract：:Red sea bream iridovirus (RSIV) of the Iridoviridae family is a causative agent of lethal infections in many cultured marine fish species in southwestern Japan. RSIV-induced apoptosis was divided as follows: (1). cell shrinkage and rounding at the early apoptotic stage, (2). cell enlargement at the middle apoptotic st...

journal_title：Virology

authors： Imajoh M,Sugiura H,Oshima S

更新日期：2004-05-01 00:00:00

abstract：:Experiments were undertaken to determine if the sera of avian sarcoma virus-infected 15I5 X 7(2) chickens exhibit antibody reactivity for species of endogenous retroviral envelope glycoprotein expressed in 15I5 X 7(2) fibroblasts. Two viruses were used for infection of the 15I5 X 7(2) chickens, Pr-A and cl. 85; the en...

journal_title：Virology

authors： Halpern MS,Deery DT,Flores LJ,Fujita DJ,Mason WS

更新日期：1983-01-30 00:00:00

abstract：:The oncogenic potential of human papillomavirus type 18 which is found in a significant number of cervical and penile cancer biopsies was tested in primary human keratinocytes derived from neonatal foreskin. Viral DNA and a gene for resistance to neomycin were introduced into these cells by calcium phosphate transfect...

journal_title：Virology

authors： Kaur P,McDougall JK

更新日期：1989-11-01 00:00:00

abstract：:The genome of the Bacillus subtilis phage SP01 contains a 12.6-kilobase-pair terminal redundancy. The transcription units in this region, which are utilized in vitro by B. subtilis RNA polymerase holoenzme, have been mapped. In vitro transcripts were separated and characterized by gel electrophoresis. Since there is s...

journal_title：Virology

authors： Brennan SM,Chelm BK,Romeo JM,Geiduschek EP

更新日期：1981-06-01 00:00:00

abstract：:The N-terminal portion of the reovirus cell attachment protein sigma 1 has recently been shown to possess intrinsic oligomerization and virion-anchoring functions. Sequence analysis of the sigma 1 proteins of the three reovirus serotypes has revealed the presence of distinct structural domains within this region: a te...

journal_title：Virology

authors： Leone G,Mah DC,Lee PW

更新日期：1991-05-01 00:00:00

abstract：:The orthopoxvirus serpin SPI-3 is N-glycosylated and suppresses fusion between infected cells. Although SPI-3 contains motifs conserved in inhibitory serpins, no proteinase inhibition by SPI-3 has been demonstrated, and mutations within the serpin reactive center loop (RCL) do not affect the ability to regulate cell f...

journal_title：Virology

authors： Turner PC,Baquero MT,Yuan S,Thoennes SR,Moyer RW

更新日期：2000-07-05 00:00:00

abstract：:The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...

journal_title：Virology

authors： Mohammadzadeh N,Follack TB,Love RP,Stewart K,Sanche S,Chelico L

更新日期：2019-01-15 00:00:00

abstract：:The Ns strain of Cucumber mosaic virus (CMV) induces hypersensitive response (HR) on Nicotiana tabacum cv. Xanthi-nc and on Nicotiana glutinosa. The genetic determinant of the HR induction was localized earlier to amino acid 461 of the 1a protein. The 3D structure of the 1a protein is still unknown and building a homo...

journal_title：Virology

authors： Salánki K,Gellért A,Náray-Szabó G,Balázs E

更新日期：2007-02-05 00:00:00

abstract：:The preS(21-47) sequence of the hepatitis B virus (HBV) envelope protein is involved in binding of the virus to cell receptors. A protein similarity search revealed a partial homology between this sequence and a segment of the human immunoglobulin A (IgA) heavy chain constant region, suggesting that the cell attachmen...

journal_title：Virology

authors： Neurath AR,Strick N

更新日期：1990-10-01 00:00:00

abstract：:The physical stability of virions of Cucumber mosaic virus (CMV) mutants was investigated to determine if relative stability correlated with efficiency of aphid transmission. Virion stability was evaluated by a urea disruption assay and by testing the infectivity of virus following purifications. All viruses were infe...

journal_title：Virology

authors： Ng JC,Josefsson C,Clark AJ,Franz AW,Perry KL

更新日期：2005-02-05 00:00:00

abstract：:Virulence markers to distinguish high from low virulence bovine viral diarrhea virus genotype 2 isolates have not been previously reported. The objective of this study was to identify virulence markers by evaluating the primary and secondary structures of the 5'-untranslated region of low and high virulence bovine vir...

journal_title：Virology

authors： Topliff CL,Kelling CL

更新日期：1998-10-10 00:00:00

abstract：:The target for neutralizing antibodies against human immunodeficiency virus (HIV) is the trimeric Env protein on the native virion. Conserved neutralizing epitopes of receptor binding sites are located in the recessed core of the Env protein, partially masked by glycosylations and variable loops. In this study, we hav...

journal_title：Virology

authors： Kang SM,Quan FS,Huang C,Guo L,Ye L,Yang C,Compans RW

更新日期：2005-01-05 00:00:00

abstract：:The matrix (MA) proteins of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) are known to be important for the targeting and assembly of lentiviral proteins. The objective of the present study was to determine whether the MA protein of HIV-1 was sufficient for particle assembly and r...

journal_title：Virology

authors： Giddings AM,Ritter GD Jr,Mulligan MJ

更新日期：1998-08-15 00:00:00

abstract：:The single-cell ELISPOT assay was used to determine the frequency and isotype commitment of virus-specific antibody-forming cells (AFC) at different anatomical locations following intranasal Sendai virus infection of C57BL/6 and 129/Sv mice. AFC responses in the mediastinal and cervical lymph nodes showed sharp increa...

journal_title：Virology

authors： Sangster M,Hyland L,Sealy R,Coleclough C

更新日期：1995-02-20 00:00:00

abstract：:The nucleotide sequence of a highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Transfection of MARC-145 cells with capped in vitro transcripts derived from a full-length cDNA clone of the viral genome resulted in infectious PRRSV with growth characteristics similar to that o...

journal_title：Virology

authors： Truong HM,Lu Z,Kutish GF,Galeota J,Osorio FA,Pattnaik AK

更新日期：2004-08-01 00:00:00

abstract：:We have constructed chimeric papillomavirus-like particles (CVLPs) by replacing the 34-carboxy-terminal amino acids of the HPV 16 L1 protein with various parts of the HPV 16 E7 protein. Chimeric proteins were expressed by recombinant baculoviruses and analyzed by electron microscopy for their ability to assemble into ...

journal_title：Virology

authors： Müller M,Zhou J,Reed TD,Rittmüller C,Burger A,Gabelsberger J,Braspenning J,Gissmann L

更新日期：1997-07-21 00:00:00

abstract：:We report the complete nucleotide sequence of the bovine herpesvirus 1 (BHV-1) immediate-early gene encoding BICP4, the homolog of the ICP4 protein of herpes simplex virus. Combined with previous mapping studies, the sequence analysis revealed that the transcript for BICP4 consisted of a noncoding leader RNA (exon 1; ...

journal_title：Virology

authors： Schwyzer M,Vlcek C,Menekse O,Fraefel C,Paces V

更新日期：1993-11-01 00:00:00

abstract：:The envelope protein of yellow fever virus 17D (YFV-17D) contains a solvent-exposed RGD motif, which has led to the suggestion that integrins may function as cellular receptors for YFV-17D. We found that mutating the RGD motif to RGE had no effect on viral titers, whereas changing RGD to TGD, TGE, TAD, TAE, or RGS led...

journal_title：Virology

authors： van der Most RG,Corver J,Strauss JH

更新日期：1999-12-05 00:00:00

abstract：:A series of carboxy- and amino-terminal deletion mutations in the movement protein (MP) gene of tobacco mosaic virus (TMV) were ligated into a cloned TMV cDNA deleted for the endogenous MP gene. RNA transcripts were produced in vitro from clones carrying the various mutated MP genes. The effect of the deletion mutatio...

journal_title：Virology

authors： Gafny R,Lapidot M,Berna A,Holt CA,Deom CM,Beachy RN

更新日期：1992-04-01 00:00:00