Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.


:It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of primary fibroblasts with BPV-1 DNA. Previously detected BPV-1 genome variations in equine sarcoids are also found in sarcoid cell lines, and only variant BPV-1 genomes can transform equine cells. These equine cell lines are morphologically transformed, proliferate faster than parental cells, have an extended life span and can grow independently of substrate. These characteristics are more marked the higher the level of viral E5, E6 and E7 gene expression. These findings confirm that the virus has an active role in the induction of sarcoids and the lines will be invaluable for further studies on the role of BPV-1 in sarcoid pathology.






Yuan ZQ,Gault EA,Gobeil P,Nixon C,Campo MS,Nasir L




Has Abstract


2008-04-10 00:00:00
















  • Virion formation is required for the long-distance movement of red clover necrotic mosaic virus in movement protein transgenic plants.

    abstract::The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species. The polycistronic RNA-1 encodes the viral RNA polymerase and capsid protein (CP) and the monocistronic RNA-2 encodes the 35 kDa cell-to-cell movement protein (MP). Nicotiana benthamiana plants transformed with ...


    pub_type: 杂志文章


    authors: Vaewhongs AA,Lommel SA

    更新日期:1995-10-01 00:00:00

  • Deoxyribonucleoprotein complexes and DNA synthesis of herpes simplex virus type 1.

    abstract::Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...


    pub_type: 杂志文章


    authors: Leinbach SS,Casto JF,Pickett TK

    更新日期:1984-09-01 00:00:00

  • Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells.

    abstract::Sindbis virus (SINV) induces inflammatory and vasoactive responses that are associated with rash and arthritis in human infections. The mechanisms underlying infection-associated microvasculopathy are still unknown. We investigated whether endothelial cells infected by SINV are differentially responsive to bradykinin ...


    pub_type: 杂志文章


    authors: Rust NM,Papa MP,Scovino AM,da Silva MM,Calzavara-Silva CE,Marques ET Jr,Peçanha LM,Scharfstein J,Arruda LB

    更新日期:2012-01-05 00:00:00

  • Suppression of antigen-specific T cell proliferation by measles virus infection: role of a soluble factor in suppression.

    abstract::Measles virus infection causes a profound immunosuppression. The basis for this immunosuppression is not known. This immunosuppression could be due to virus acting directly on lymphoid cells, the production of an immunosuppressive viral product, or a lymphoid product. We have developed an antigen-specific T cell syste...


    pub_type: 杂志文章


    authors: Sun X,Burns JB,Howell JM,Fujinami RS

    更新日期:1998-06-20 00:00:00

  • Synthesis in vitro of infectious RNA copies of the virulent satellite of turnip crinkle virus.

    abstract::RNA copies, synthesized in vitro, of the virulent satellite (RNA C) of turnip crinkle virus (TCV) infect plants when coinoculated with helper virus RNA. RNA C is a small linear RNA of about 355 bases which intensifies TCV symptoms in infected plants. Full-length cDNA copies of the satellite were inserted in an express...


    pub_type: 杂志文章


    authors: Simon AE,Howell SH

    更新日期:1987-01-01 00:00:00

  • The capsid architecture of channel catfish virus, an evolutionarily distant herpesvirus, is largely conserved in the absence of discernible sequence homology with herpes simplex virus.

    abstract::Although herpesviruses have a wide host range and their genomes vary substantially in size, the nucleocapsid appears to be a conservative element of viral design. The capsid shell is icosahedrally symmetric (T = 16), and 125 nm in diameter and 15nm thick in the case of herpes simplex virus 1 (HSV-1). Channel catfish v...


    pub_type: 杂志文章


    authors: Booy FP,Trus BL,Davison AJ,Steven AC

    更新日期:1996-01-15 00:00:00

  • HPV-16 E5 down-regulates expression of surface HLA class I and reduces recognition by CD8 T cells.

    abstract::HPV-16 is the major causes of cervical cancer. Persistence of infection is a necessary event for progression of the infection to cancer. Among other factors, virus persistence is due the viral proteins fighting the immune response. HPV-16 E5 down-regulates MHC/HLA class I, which is much reduced on the cell surface and...


    pub_type: 杂志文章


    authors: Campo MS,Graham SV,Cortese MS,Ashrafi GH,Araibi EH,Dornan ES,Miners K,Nunes C,Man S

    更新日期:2010-11-10 00:00:00

  • Heterogeneity in envelope protein sequence and N-linked glycosylation among yellow fever virus vaccine strains.

    abstract::We have compared the deduced envelope (E) protein sequences of two biologically well-characterized yellow fever (YF) virus vaccine strains. The 17DD strain has been produced in Brazil for more than 50 years and used to successfully vaccinate millions of people worldwide. The 17D-213 is a candidate vaccine strain produ...


    pub_type: 杂志文章


    authors: Post PR,Santos CN,Carvalho R,Cruz AC,Rice CM,Galler R

    更新日期:1992-05-01 00:00:00

  • The transmembrane domains of the EBV-encoded latent membrane protein 1 (LMP1) variant CAO regulate enhanced signalling activity.

    abstract::Sequence variants of the Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP1) have been reported in association with EBV-linked malignancies but little is known about their effects on signalling pathways and phenotype. We have examined the ability of the nasopharyngeal carcinoma (NPC)-derived variant, CAO...


    pub_type: 杂志文章


    authors: Blake SM,Eliopoulos AG,Dawson CW,Young LS

    更新日期:2001-04-10 00:00:00

  • Conservation of DNA sequence in the predicted major late promoter regions of selected mastadenoviruses.

    abstract::The major late promoter (MLP) of the subgroup C human adenoviruses is a preeminent model for the study of the mechanisms of basal and activated transcription, both in vivo and in vitro. However, while the structure and function of the human virus MLP has been the subject of extensive investigation, the conservation of...


    pub_type: 杂志文章


    authors: Song B,Hu SL,Darai G,Spindler KR,Young CS

    更新日期:1996-06-15 00:00:00

  • Effects of antibody to the influenza A virus M2 protein on M2 surface expression and virus assembly.

    abstract::We have investigated the effect of a monoclonal antibody on influenza virus release and the cell surface expression of M2, comparing virus strains which were observed previously to be sensitive (A/Udorn) or resistant (A/WSN and A/Udorn variants) to growth inhibition by M2 antibody 14C2. Incubation of A/Udorn virus-inf...


    pub_type: 杂志文章


    authors: Hughey PG,Roberts PC,Holsinger LJ,Zebedee SL,Lamb RA,Compans RW

    更新日期:1995-10-01 00:00:00

  • Transcription mapping and characterization of 284R and 121R proteins produced from early region 3 of bovine adenovirus type 3.

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    pub_type: 杂志文章


    authors: Idamakanti N,Reddy PS,Babiuk LA,Tikoo SK

    更新日期:1999-04-10 00:00:00

  • Influenza C and D viral load in cattle correlates with bovine respiratory disease (BRD): Emerging role of orthomyxoviruses in the pathogenesis of BRD.

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    pub_type: 杂志文章


    authors: Nissly RH,Zaman N,Ibrahim PAS,McDaniel K,Lim L,Kiser JN,Bird I,Chothe SK,Bhushan GL,Vandegrift K,Neibergs HL,Kuchipudi SV

    更新日期:2020-12-01 00:00:00

  • The structure of adeno-associated virus serotype 3B (AAV-3B): insights into receptor binding and immune evasion.

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    pub_type: 杂志文章


    authors: Lerch TF,Xie Q,Chapman MS

    更新日期:2010-07-20 00:00:00

  • High viral load and CD4 lymphopenia in rhesus and cynomolgus macaques infected by a chimeric primate lentivirus constructed using the env, rev, tat, and vpu genes from HIV-1 Lai.

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    pub_type: 杂志文章


    authors: Dunn CS,Beyer C,Kieny MP,Gloeckler L,Schmitt D,Gut JP,Kirn A,Aubertin AM

    更新日期:1996-09-15 00:00:00

  • Interleukin 2-inducible T cell kinase (ITK) facilitates efficient egress of HIV-1 by coordinating Gag distribution and actin organization.

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    pub_type: 杂志文章


    authors: Schiralli Lester GM,Akiyama H,Evans E,Singh J,Gummuluru S,Henderson AJ

    更新日期:2013-02-05 00:00:00

  • Epitopes on foot-and-mouth disease virus particles. I. Topology.

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    pub_type: 杂志文章


    authors: McCullough KC,Crowther JR,Carpenter WC,Brocchi E,Capucci L,De Simone F,Xie Q,McCahon D

    更新日期:1987-04-01 00:00:00

  • Mapping nucleotides in the 126-kDa protein gene that control the differential symptoms induced by two strains of tobacco mosaic virus.

    abstract::The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...


    pub_type: 杂志文章


    authors: Shintaku MH,Carter SA,Bao Y,Nelson RS

    更新日期:1996-07-01 00:00:00

  • The evolution of porcine reproductive and respiratory syndrome virus: quasispecies and emergence of a virus subpopulation during infection of pigs with VR-2332.

    abstract::GP5, the principal envelope glycoprotein of porcine reproductive and respiratory syndrome virus (PRRSV), contains a hypervariable region within the ectodomain which is responsible for generating diversity in field isolates. The purpose of this study was to gain insight into the possible origin of this diversity by fol...


    pub_type: 杂志文章


    authors: Rowland RR,Steffen M,Ackerman T,Benfield DA

    更新日期:1999-07-05 00:00:00

  • Activation of protein kinase R by hepatitis C virus RNA-dependent RNA polymerase.

    abstract::Hepatitis C virus (HCV) was shown to activate protein kinase R (PKR), which inhibits expression of interferon (IFN) and IFN-stimulated genes by controlling the translation of newly transcribed mRNAs. However, it is unknown exactly how HCV activates PKR. To address the molecular mechanism(s) of PKR activation mediated ...


    pub_type: 杂志文章


    authors: Suzuki R,Matsuda M,Shimoike T,Watashi K,Aizaki H,Kato T,Suzuki T,Muramatsu M,Wakita T

    更新日期:2019-03-01 00:00:00

  • Detection of the readthrough protein of barley yellow dwarf virus.

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    pub_type: 杂志文章


    authors: Cheng SL,Domier LL,D'Arcy CJ

    更新日期:1994-08-01 00:00:00

  • Stability of rice yellow mottle virus and cellular compartmentalization during the infection process in Oryza sativa (L.).

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    pub_type: 杂志文章


    authors: Brugidou C,Opalka N,Yeager M,Beachy RN,Fauquet C

    更新日期:2002-05-25 00:00:00

  • Betanodavirus induces phosphatidylserine exposure and loss of mitochondrial membrane potential in secondary necrotic cells, both of which are blocked by bongkrekic acid.

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    pub_type: 杂志文章


    authors: Chen SP,Yang HL,Her GM,Lin HY,Jeng MF,Wu JL,Hong JR

    更新日期:2006-04-10 00:00:00

  • Productive infection of CD34+-cell-derived megakaryocytes by X4 and R5 HIV-1 isolates.

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    pub_type: 杂志文章


    authors: Voulgaropoulou F,Pontow SE,Ratner L

    更新日期:2000-03-30 00:00:00

  • Transcript mapping of Abutilon mosaic virus, a geminivirus.

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    pub_type: 杂志文章


    authors: Frischmuth S,Frischmuth T,Jeske H

    更新日期:1991-12-01 00:00:00

  • The insertion domain of the duck hepatitis B virus core protein plays a role in nucleocapsid assembly.

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    pub_type: 杂志文章


    authors: Guo H,Aldrich CE,Saputelli J,Xu C,Mason WS

    更新日期:2006-09-30 00:00:00

  • Identification of three genes nonessential for growth in cell culture near the right terminus of the unique sequences of long component of herpes simplex virus 1.

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    pub_type: 杂志文章


    authors: Barker DE,Roizman B

    更新日期:1990-08-01 00:00:00

  • Protection from pathogenic SIVmac challenge following short-term infection with a nef-deficient attenuated virus.

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    pub_type: 杂志文章


    authors: Norley S,Beer B,Binninger-Schinzel D,Cosma C,Kurth R

    更新日期:1996-05-01 00:00:00

  • Induction of circular episomes during rescue and replication of adeno-associated virus in experimental models of virus latency.

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    pub_type: 杂志文章


    authors: Musatov SA,Scully TA,Dudus L,Fisher KJ

    更新日期:2000-09-30 00:00:00

  • A critical Sp1 element in the rhesus rhadinovirus (RRV) Rta promoter confers high-level activity that correlates with cellular permissivity for viral replication.

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    pub_type: 杂志文章


    authors: DeMaster LK,Rose TM

    更新日期:2014-01-05 00:00:00