Abstract:
:A series of carboxy- and amino-terminal deletion mutations in the movement protein (MP) gene of tobacco mosaic virus (TMV) were ligated into a cloned TMV cDNA deleted for the endogenous MP gene. RNA transcripts were produced in vitro from clones carrying the various mutated MP genes. The effect of the deletion mutations on local and systemic movements of the infection was evaluated. Deletion of 9 or 33 amino acids from the carboxy terminus of the movement protein did not effect cell-to-cell movement as reflected by local lesion formation on Nicotiana tabacum cv. Xanthi NN plants. Deletion of 55 amino acids resulted in impaired MP that supported the formation of local lesions of 1 mm in diameter compared to lesions of 3-5 mm caused by the wild-type MP. Deletion of 74 amino acids (or more) from the carboxy terminus resulted in a protein that could not support virus movement. Modified viruses that contained repeated sequences in the 3' region of the MP gene lost the repeated sequences during replication and reverted to the wild type. This was evidenced by the size of the MP produced and by sequence analysis of reverse-transcribed PCR-amplified products, following infection by the modified virus. MP deleted for as few as 3 amino acids at the amino terminus could not support virus movement thus indicating that the amino-terminal domain is critical for MP activity.
journal_name
Virologyjournal_title
Virologyauthors
Gafny R,Lapidot M,Berna A,Holt CA,Deom CM,Beachy RNdoi
10.1016/0042-6822(92)90452-usubject
Has Abstractpub_date
1992-04-01 00:00:00pages
499-507issue
2eissn
0042-6822issn
1096-0341journal_volume
187pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Outbreaks of avian influenza virus H5N8 first occurred in 2014, and spread to poultry farms in Korea. Although there was no report of human infection by this subtype, it has the potential to threaten human public health. Therefore, we evaluated the pathogenesis of H5N8 viruses in ferrets. Two representative Korean H5N...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.02.042
更新日期:2015-07-01 00:00:00
abstract::Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90047-f
更新日期:1991-01-01 00:00:00
abstract::The yellow fever 17D virus is attenuated and used for human vaccination. Two of its substrains, 17D-204 and 17DD, are used for vaccine production. One of the remarkable properties of this vaccine is limited viral replication in the host but with significant dissemination of the viral mass, yielding a robust and long-l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00583-x
更新日期:2003-11-10 00:00:00
abstract::Nucleotide sequence determination of the coat protein cistron of the alfalfa mosaic virus (AIMV) temperature-sensitive mutant, Tbts 7 (uv) revealed a small number of point mutations of which only one results in the replacement of an amino acid: the asparagine residue at position 126 is replaced by an aspartate residue...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90609-0
更新日期:1989-08-01 00:00:00
abstract::Like human adenovirus type 5 (HAV5), bovine adenovirus type 3 (BAV3) early region 1 (E1) consists of E1A and E1B transcriptional units. In order to characterize BAV3 E1 proteins and to isolate a cell line of bovine origin that expresses BAV3 E1, polyclonal antibodies specific to E1A, E1B-157R, and E1B-420R were raised...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1389
更新日期:2002-03-30 00:00:00
abstract::The identification of mechanisms that prevent infection with human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV) would facilitate the development of an effective AIDS vaccine. In time-course experiments, protection against detectable superinfection with homologous wild-type SIV was achieved withi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.09.026
更新日期:2004-12-05 00:00:00
abstract::Treatment with HIV attachment inhibitors (AIs) can select for escape mutants throughout the viral envelope. We report on three such mutations: F423Y (gp120 CD4 binding pocket) and I595F and K655E (gp41 ectodomain). Each displayed decreased sensitivity to the AI BMS-488043 and earlier generation AIs, along with increas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.033
更新日期:2010-07-05 00:00:00
abstract::Reactivation of latent herpes simplex virus 2 (HSV-2) infections can be characterized by episodic recurrent genital lesions and/or viral shedding. We hypothesize that infected (HSV-2(pos)) asymptomatic individuals have acquired T cell responses to specific HSV-2 antigen(s) that may be an important factor in controllin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.07.018
更新日期:2014-09-01 00:00:00
abstract::Differing reports exist about the replication of human enteric adenoviruses (EnAds) in various cell lines. There was a suggestion that EnAds are defective, do not grow on primary human diploid cells, and behave like Ad host-range mutants, i.e., they require early gene products from other Ad types for efficient growth....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90072-y
更新日期:1990-01-01 00:00:00
abstract::Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.05.023
更新日期:2014-09-01 00:00:00
abstract::Using reverse genetics methods, we constructed three different transfectant influenza A viruses encoding an Ld-restricted, nine amino-acid-long fragment, corresponding to amino-acid residues 876-884, of beta-galactosidase (beta-gal). Sequences encoding this epitope were nested within the hemagglutinin (HA) or neuramin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9330
更新日期:1998-09-15 00:00:00
abstract::The nonstructural rotavirus receptor glycoprotein NS28 is 175 amino acids long and oriented in the RER membrane with the NH2 terminus on the luminal side and approximately 131 amino acids accessible from the cytoplasmic side. Au et al. (1988) have demonstrated that NS28 is able to interact with rotavirus single-shelle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90515-1
更新日期:1989-07-01 00:00:00
abstract::Binding of simian virus 40 (SV40) large T antigen to human and calf thymus topoisomerase I (topo I) was readily detected by using modified enzyme-linked immunosorbent assays and immunoblots. In addition to WT T antigen, binding could also be readily demonstrated with T antigen fragments from the amino-terminal region ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0433
更新日期:1996-08-15 00:00:00
abstract::Our recent results demonstrated that bile acids facilitate virus escape from the endosomes into the cytoplasm for successful replication of porcine enteric calicivirus (PEC). We report a novel finding that bile acids can be substituted by cold treatment for endosomal escape and virus replication. This endosomal escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.04.022
更新日期:2015-09-01 00:00:00
abstract::Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90037-8
更新日期:1989-03-01 00:00:00
abstract::The E7 oncoprotein of high risk human papillomavirus type 16 (HPV16) binds and inactivates the retinoblastoma (RB) family of proteins. Our previous studies suggested that HPV16 E7 enters the nucleus via a novel Ran-dependent pathway independent of the nuclear import receptors (Angeline, M., Merle, E., and Moroianu, J....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.037
更新日期:2009-01-05 00:00:00
abstract::The Psu (Polarity suppression) protein of satellite bacteriophage P4 was first characterized as an anti-terminator of transcription termination in Escherichia coli. Psu is also a structural component of mature P4 capsids, where it is present as a decoration protein. Psu is located externally on the capsid surface, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1307
更新日期:1993-06-01 00:00:00
abstract::The ultrastructure of the mature and immature poxvirus Molluscum contagiosum has been examined by electron microscopy. With freeze-fracturing one fracture plane was observed in the viral envelope. During the conversion of immature into mature virus, particles inside the envelope become partly rearranged into parallel ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90215-x
更新日期:1977-11-01 00:00:00
abstract::Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90211-9
更新日期:1990-03-01 00:00:00
abstract::Computational analysis of human adenovirus type 4 (HAdV-E4), a pathogen that is the only HAdV member of species E, provides insights into its zoonotic origin and molecular adaptation. Its genome encodes a domain of the major capsid protein, hexon, from HAdV-B16 recombined into the genome chassis of a simian adenovirus...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.05.014
更新日期:2013-09-01 00:00:00
abstract::Coronavirus small envelope protein E has two known biological functions: it plays a pivotal role in virus envelope formation, and the murine coronavirus E protein induces apoptosis in E protein-expressing cultured cells. The E protein is an integral membrane protein. Its C-terminal region extends cytoplasmically in th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0818
更新日期:2001-03-15 00:00:00
abstract::The subgroup II luteovirus barley yellow dwarf virus-RPV (BYDV-RPV) acts as a helper virus for a satellite RNA (satRPV RNA). The subgroup II luteovirus beet western yellows virus (BWYV) and the ST9-associated RNA (ST9a RNA), a BWYV-associated RNA that encodes a polymerase similar to those of subgroup I luteoviruses, w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8532
更新日期:1997-05-12 00:00:00
abstract::Activation of the human cytomegalovirus (HCMV) origin of replication (oriLyt) was previously demonstrated in transient transfection assays in permissive human fetal fibroblasts and nonpermissive Vero cells, and shown to require six viral proteins that function at the replication fork plus a number of HCMV products tha...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00005-9
更新日期:2003-04-10 00:00:00
abstract::In response to oncogenic insults, normal human cells execute a defense response that culminates in cellular suicide, apoptosis. Normal human diploid fibroblasts expressing the human papillomavirus type 16 (HPV-16) E7 oncoprotein are predisposed to apoptosis when they are deprived of growth factors. Even though a domin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.11.008
更新日期:2004-02-05 00:00:00
abstract::Langat (LGT) flavivirus, derived from infectious full-length cDNA clone 636, was investigated for its apoptotic activities in mouse neuroblastoma (Neuro-2a) and simian kidney (Vero and LLC-MK(2)) cells. The hallmark of apoptosis, cleavage of cellular DNA, was observed 48 h after infection of Vero, LLC-MK(2), and Neuro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0980
更新日期:2001-08-01 00:00:00
abstract::Tombusviruses replicate on pre-existing organelles such as peroxisomes or mitochondria, the membranes of which become extensively reorganized into multivesicular bodies (MVBs) during the infection process. Cucumber necrosis virus (CNV) has previously been shown to replicate in association with peroxisomes in yeast. We...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.12.035
更新日期:2014-03-01 00:00:00
abstract::Adenovirus 40 (Ad40) is defective for growth in tissue culture but is complemented when the Ad2/5 or Ad12 E1B 55K protein is supplied in trans. Ad40 E1B mRNA has not been detected in E1-transformed cells, or at early times in lytically infected cells. In cells constitutively expressing the E1B region of Ad2, Ad40 E1B ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90478-t
更新日期:1991-03-01 00:00:00
abstract::Binding of interferons (IFNs) to their cell surface receptors stimulates rapid translocation of cytoplasmic proteins to the nucleus and the expression of a variety of cellular genes within minutes. Translocated proteins subsequently bind to the interferon-stimulated response element (ISRE) located in the promoters of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91207-b
更新日期:1992-09-01 00:00:00
abstract::In a previous study, we observed that hepatitis C virus (HCV) core protein specifically inhibits translation initiated by an HCV internal ribosome entry site (IRES). To investigate the mechanism by which down-regulation of HCV translation occurs, a series of mutations were introduced into the IRES element, as well as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.013
更新日期:2006-02-20 00:00:00
abstract::From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90357-x
更新日期:1984-10-30 00:00:00