Abstract:
:In a previous study, we observed that hepatitis C virus (HCV) core protein specifically inhibits translation initiated by an HCV internal ribosome entry site (IRES). To investigate the mechanism by which down-regulation of HCV translation occurs, a series of mutations were introduced into the IRES element, as well as the core protein, and their effect on IRES activity examined in this study. We found that expression of the core protein inhibits HCV translation possibly by binding to a stem-loop IIId domain, particularly a GGG triplet within the hairpin loop structure of the domain, within the IRES. Basic-residue clusters located at the N-terminus of the core protein have an inhibitory effect on HCV translation, and at least one of three known clusters is required for inhibition. We propose a model in which competitive binding of the core protein for the IRES and 40S ribosomal subunit regulates HCV translation.
journal_name
Virologyjournal_title
Virologyauthors
Shimoike T,Koyama C,Murakami K,Suzuki R,Matsuura Y,Miyamura T,Suzuki Tdoi
10.1016/j.virol.2005.10.013subject
Has Abstractpub_date
2006-02-20 00:00:00pages
434-45issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(05)00649-5journal_volume
345pub_type
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