Abstract:
:Rice dwarf virus (RDV) spreads, along actin-based filopodia, as one of the ways of its transport, from infected to non-infected cells of its vector insect via tubules that are composed of the viral nonstructural protein Pns10 and contain viral particles. An inhibitor that perturbs the endomembrane system, namely, brefeldin A, and butanedione monoxime, an inhibitor of myosin motor activity, abolished the formation of such tubules, preventing the direct cell-to-cell spread of RDV without any significant effects on the production of viral particles. The microtubule-depolymerizing drug NOC, which had a negligible effect on the formation and protrusion of such tubules from cells, did not inhibit the intercellular spread of RDV. Our observations suggest that the endomembrane system and myosin motors, but not microtubule, are required for the Pns10 tubule formation and are, therefore, involved in the secondary infection by RDV of adjacent cells.
journal_name
Virologyjournal_title
Virologyauthors
Wei T,Shimizu T,Omura Tdoi
10.1016/j.virol.2007.10.034subject
Has Abstractpub_date
2008-03-15 00:00:00pages
349-56issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(07)00708-8journal_volume
372pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::In genetically susceptible C57BL/6 mice the LP-BM5 isolate of murine retroviruses causes profound splenomegaly, lymphadenopathy, hypergammaglobulinemia, and an immunodeficiency syndrome bearing many similarities to the pathologies seen in AIDS. Because of these similarities, which also include terminal B cell lymphoma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8970
更新日期:1998-02-15 00:00:00
abstract::The effect of adenovirus infection or transformation on the DNA replication of Minute Virus of Mice (MVM) was studied in human fibroblast cell lines. In WI38, HeLa, and 293 cells MVM infection allowed production of viral NS-1 and capsid proteins with or without adenovirus 2 (Ad2) co-infection. However, MVM DNA replica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90010-o
更新日期:1990-06-01 00:00:00
abstract::The transmembrane subunit (gp41) of the HIV Env glycoprotein contains conserved neutralizing epitopes which are not well-exposed in wild-type HIV Env proteins. To enhance the exposure of these epitopes, a chimeric protein, HA/gp41, in which the gp41 of HIV-1 89.6 envelope protein was fused to the C-terminus of the HA1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.04.012
更新日期:2006-08-15 00:00:00
abstract::HIV-1 virus entry into target cells requires the envelope glycoprotein (Env) to first bind the primary receptor, CD4 and subsequently the co-receptor. Antibody access to the co-receptor binding site (CoRbs) in the pre-receptor-engaged state, prior to cell attachment, remains poorly understood. Here, we have demonstrat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.04.002
更新日期:2018-06-01 00:00:00
abstract::Mutational analyses were carried out to investigate whether the nuclear inclusion protein a (NIa) C-terminal amino acids of turnip mosaic potyvirus play any roles in the cis-cleavage between NIa and NIb. The processing rate of the NIa-NIb junction sequence was decreased significantly by either V240D or Q243A mutation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8938
更新日期:1998-02-01 00:00:00
abstract::The shuttle vector plasmid pZ189 was used as a target for mutagenesis in COS-1 cells. Complex mutations were analyzed in 5 plasmids that were recovered from noninfected cells and 15 plasmids that were recovered from cells that were infected with herpes simplex virus type-1 (HSV-1). Complex mutations in noninfected cel...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90895-i
更新日期:1991-04-01 00:00:00
abstract::Two genetically distinct infectious recombinant virus clones (pMLV, constructed from Ingelvac PRRS MLV and pMN184, constructed from virulent strain MN184) were developed to study attenuation of contemporary porcine reproductive and respiratory syndrome virus (PRRSV) strain MN184. Two reciprocal chimeric clones (pMLVOR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.09.032
更新日期:2008-02-20 00:00:00
abstract::Purified preparations of the Helminthosporium victoriae 190S (Hv190S) virus contain two sedimenting components that differ in capsid structure. The slower sedimenting component (190S-1) contained p88 and p83 as the major capsid proteins; the faster component (190S-2) contained p88 and p78. Previous peptide-mapping stu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90520-y
更新日期:1992-06-01 00:00:00
abstract::New sequences have been obtained by successive overlapping RT-PCR extensions from the pol region of a retroviral RNA (multiple sclerosis-associated retroviral element, MSRV) amplified in retrovirus-like particles from patients with multiple sclerosis. gag and pol sequences are related to type C oncoviruses, whereas th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9792
更新日期:1999-07-20 00:00:00
abstract::The interaction of parvovirus minute virus of mice (prototype strain, MVMp) with simian virus 40 (SV40)-transformed human cells (NB-E) was investigated by means of transfection with MVMp molecular clones derived from the infectious recombinant plasmid (pMM984). pMM984 inhibits stable transformation of NB-E cells to ge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90110-d
更新日期:1990-02-01 00:00:00
abstract::The complete nucleotide sequence of the nucleocapsid (N) gene of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus of plant rhabdoviruses, has been determined from cDNA clones of both the viral genomic and messenger RNAs. The previously identified N gene was adjacent to the 3' leader ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1438
更新日期:1994-08-01 00:00:00
abstract::Retroviral nucleocapsid (NC) proteins are small Gag-derived products containing one or two zinc finger motifs that mediate genomic RNA packaging into virions. In this study, we addressed the role of the feline immunodeficiency virus (FIV) NC protein in the late stages of virus replication by analyzing the assembly phe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.06.019
更新日期:2004-09-15 00:00:00
abstract::Five plaque-forming viruses (Pbi viruses) of the unicellular, eukaryotic, exsymbiotic Chlorella-like green alga strain Pbi were isolated from fresh water collected in Germany. The viruses were compared to two previously characterized plaque-forming viruses (NC64A viruses) of Chlorella strain NC64A. The Pbi viruses do ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90063-3
更新日期:1988-11-01 00:00:00
abstract::We have studied the assembly of human immunodeficiency virus (HIV-1) Gag-B-galactosidase (Gag-B-gal; GBG) fusion proteins into HIV particles in the presence of HIV Gag proteins. Release of fusion proteins from cells was measured by assay of media versus cellular B-gal activities and was dependent on co-expression of u...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1215
更新日期:1994-05-01 00:00:00
abstract::In order to define factors involved in very late Autographa californica nucleopolyhedrovirus (AcMNPV) gene function, random mutagenesis of a baculovirus recombinant (AcUW1.lacZ) by 5'-bromodeoxyuridine treatment was performed. Five viruses were selected with deficiencies in very late gene expression. These were charac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0121
更新日期:1996-03-01 00:00:00
abstract::Aside from a common gene organization shared with other picornaviruses, hepatitis A virus (HAV) is characterized by its slow-growth phenotype, the inability to shut off host macromolecular synthesis, and, in general, lack of cytopathic (cp) effects in permissive cell cultures. Nevertheless, several cp HAV strains have...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0070
更新日期:2000-01-05 00:00:00
abstract::Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.029
更新日期:2009-03-15 00:00:00
abstract::The nucleic acid of carnation cryptic virus has been identified as double-stranded RNA on the basis of (i) susceptibility to RNase in 0.1 x SSC (SSC = 0.15 M NaCl-0.015 M sodium citrate buffer, pH 7.0) and resistance to RNase in 2 x SSC and to DNase, (ii) reaction with an antiserum containing antibodies to dsRNA, and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90124-0
更新日期:1981-12-01 00:00:00
abstract::Baculoviruses encode fibroblast growth factor (vfgf) homologs whose function during virus infection is unknown. We constructed a recombinant bacmid of Autographa californica M nucleopolyhedrovirus (AcMNPV) lacking a functional vfgf and characterized it in two insect cell lines. The kinetics of budded virus production ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.01.016
更新日期:2006-03-15 00:00:00
abstract::The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6-methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8936
更新日期:1998-01-20 00:00:00
abstract::The primer binding site (PBS) is involved in two stages during the reverse transcription of the retroviral RNA genome. In the early stage, the PBS provides complementary sequences through which tRNA(Lys,3) binds the viral RNA genome to initiate minus-strand DNA synthesis; in the later stages, complementarity between t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0317
更新日期:1996-06-15 00:00:00
abstract::The complete genome sequence of the Liaoning isolate of the Antheraea pernyi multinucleocapsid nucleopolyhedrovirus (AnpeMNPV) was determined. The viral genome size is 126,246 bp, relatively GC-rich (53.5% G+C), with 145 predicted open reading frames (ORFs) of more than 50 amino acids, accounting for more than 97% of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.04.027
更新日期:2007-09-30 00:00:00
abstract::Glycoprotein C (gC) of pseudorabies virus, a swine herpesvirus, initiates virus attachment by binding to heparan sulfate (HS) linked to proteoglycans (HSPGs) on the cell surface. This interaction facilitates a required step in virus entry, the binding to a non-HS coreceptor, likely by another viral glycoprotein, gD. W...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00024-7
更新日期:2003-03-01 00:00:00
abstract::Human immunodeficiency virus (HIV) Gag proteins are assembled into virus particles and then cleaved by the virion-associated HIV protease. Concomitant with Gag processing, doughnut-like HIV particles (the immature form) are converted to particles containing condensed cores (the mature form). Here we describe the in vi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0370
更新日期:2000-07-05 00:00:00
abstract::The DNA extracted from bacteriophage 643, which infects group N strains of Streptococci, could be separated into four components by electrophoresis on agarose gels. Electron microscopy established the predominant form of the DNA to be a relaxed, circular molecule of molecular weight 14.9 X 10(6). The four species obse...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90406-9
更新日期:1984-03-01 00:00:00
abstract::Infection of different human epithelial cell lines with human respiratory syncytial virus (HRSV) revealed significant differences in the electrophoretic mobility of the viral attachment glycoprotein (G). Cell-type specific differences in G protein glycosylation were observed with certain lectins and sugar-specific rea...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0379
更新日期:1996-07-15 00:00:00
abstract::The genetic structure of the population of Watermelon mosaic virus (WMV) in Spain was analysed by the biological and molecular characterisation of isolates sampled from its main host plant, melon. The population was a highly homogeneous one, built of a single pathotype, and comprising isolates closely related genetica...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.10.002
更新日期:2004-01-05 00:00:00
abstract::The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9379
更新日期:1998-10-25 00:00:00
abstract::Encephalitis is a rare, but serious complication from vaccination against smallpox using replication competent strains of vaccinia virus. In this report we describe mutants of vaccinia virus, containing N-terminal deletions of the vaccinia virus interferon resistance gene, E3L, that are attenuated for neuropathogenesi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.006
更新日期:2005-03-15 00:00:00
abstract::The HIV-1 Nef protein causes a decrease in major histocompatibility complex (MHC) class I and CD4 molecule expression on the cell surface. To determine if Nef can affect components of the innate immune response, we assessed the ability of Nef to alter the cell surface expression of human CD1d. In cells co-expressing C...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.04.020
更新日期:2005-07-05 00:00:00