当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Antigenic properties of a transport-competent influenza HA/HIV Env chimeric protein.

Antigenic properties of a transport-competent influenza HA/HIV Env chimeric protein.

Abstract：

:The transmembrane subunit (gp41) of the HIV Env glycoprotein contains conserved neutralizing epitopes which are not well-exposed in wild-type HIV Env proteins. To enhance the exposure of these epitopes, a chimeric protein, HA/gp41, in which the gp41 of HIV-1 89.6 envelope protein was fused to the C-terminus of the HA1 subunit of the influenza HA protein, was constructed. Characterization of protein expression showed that the HA/gp41 chimeric proteins were expressed on cell surfaces and formed trimeric oligomers, as found in the HIV Env as well as influenza HA proteins. In addition, the HA/gp41 chimeric protein expressed on the cell surface can also be cleaved into 2 subunits by trypsin treatment, similar to the influenza HA. Moreover, the HA/gp41 chimeric protein was found to maintain a pre-fusion conformation. Interestingly, the HA/gp41 chimeric proteins on cell surfaces exhibited increased reactivity to monoclonal antibodies against the HIV Env gp41 subunit compared with the HIV-1 envelope protein, including the two broadly neutralizing monoclonal antibodies 2F5 and 4E10. Immunization of mice with a DNA vaccine expressing the HA/gp41 chimeric protein induced antibodies against the HIV gp41 protein and these antibodies exhibit neutralizing activity against infection by an HIV SF162 pseudovirus. These results demonstrate that the construction of such chimeric proteins can provide enhanced exposure of conserved epitopes in the HIV Env gp41 and may represent a novel vaccine design strategy for inducing broadly neutralizing antibodies against HIV.

journal_name

Virology

journal_title

Virology

authors

Ye L,Sun Y,Lin J,Bu Z,Wu Q,Jiang S,Steinhauer DA,Compans RW,Yang C

doi

10.1016/j.virol.2006.04.012

subject

Has Abstract

pub_date

2006-08-15 00:00:00

pages

74-85

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(06)00264-9

journal_volume

352

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Analysis of nucleotides 13-96 of the human parainfluenza virus type 3 antigenomic promoter reveals positive- and negative-acting replication elements.

    abstract：:During replication of human parainfluenza virus type 3 (HPIV3), the 96-nucleotide antigenomic promoter (AGP) of HPIV3 directs the synthesis of genomic RNA. Previous work showed that nucleotides 1-12 were critical in promoting replication of an HPIV3 minireplicon, but nucleotides 13-96 were not investigated. In this st...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.08.003

    authors： Gander JR,Schwan LM,Hoffman MA

    更新日期：2011-10-25 00:00:00

  • Protein S and Gas6 induce efferocytosis of HIV-1-infected cells.

    abstract：:Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.12.025

    authors： Chua BA,Ngo JA,Situ K,Ramirez CM,Nakano H,Morizono K

    更新日期：2018-02-01 00:00:00

  • Determination of a splice acceptor site of pX gene in HTLV-I infected cells.

    abstract：:The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine e...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90404-0

    authors： Okamoto T,Josephs SF,Kawanishi M,Wong-Staal F

    更新日期：1985-06-01 00:00:00

  • Effect of 5' and 3' terminal sequences, overall length, and coding capacity on the accumulation of defective RNAs associated with broad bean mottle bromovirus in planta.

    abstract：:Broad bean mottle bromovirus (BBMV) was shown to accumulate RNA2-derived defective interfering (DI) RNAs [Romero et al., Virology 194, 576-584 (1993); Pogany et al., Virology 212, 574-586 (1995)]. In this work, we utilize three sets of BBMV RNA2-derived artificial DI RNA constructs to determine factors that affect the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8377

    authors： Pogany J,Romero J,Bujarski JJ

    更新日期：1997-02-17 00:00:00

  • The bovine papilloma virus E1 protein has ATPase activity essential to viral DNA replication and efficient transformation in cells.

    abstract：:The bovine papilloma virus (BPV) E1 protein essential to viral DNA replication has recently been shown to associate via direct protein-DNA interactions with the viral origin of replication and to be an ATP-dependent helicase. We show here that in accordance with the latter function, the E1 gene product has intrinsic A...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1544

    authors： MacPherson P,Thorner L,Parker LM,Botchan M

    更新日期：1994-10-01 00:00:00

  • Human T cell leukemia virus type I Tax activates CD40 gene expression via the NF-kappa B pathway.

    abstract：:The human T cell leukemia virus type I (HTLV-I) is an oncogenic retrovirus that is etiologically linked to the genesis of adult T cell leukemia (ATL) as well as HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Emerging evidence suggests that the pathogenicity of HTLV-I involves deregulated activati...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.12.008

    authors： Harhaj EW,Harhaj NS,Grant C,Mostoller K,Alefantis T,Sun SC,Wigdahl B

    更新日期：2005-03-01 00:00:00

  • Structure of the bacteriophage lambda cohesive end site: bent DNA on both sides of the site, cosN, at which terminase introduces nicks during chromosome maturation.

    abstract：:Packaging of lambda DNA is mediated by the phage-encoded enzyme, terminase, which acts at a site termed cos. cos consists of cosB, the site where terminase binds lambda DNA, and cosN, the site where nicks are introduced to generate the cohesive ends of virion DNA. cos contains multiple binding sites for gpNu1, the sma...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90085-6

    authors： Yeo A,Kosturka LD,Feiss M

    更新日期：1990-01-01 00:00:00

  • Gut microbiota is associated with protection against Marek's disease virus infection in chickens.

    abstract：:Marek's Disease Virus (MDV) infects chickens via respiratory route and causes lymphomas in internal organs including gastrointestinal tract. MDV infection causes a shift in the gut microbiota composition. However, interactions between the gut microbiota and immune responses against MDV infection are not well understoo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.10.011

    authors： Bavananthasivam J,Astill J,Matsuyama-Kato A,Taha-Abdelaziz K,Shojadoost B,Sharif S

    更新日期：2021-01-15 00:00:00

  • A collaborative report: rhinoviruses--extension of the numbering system from 89 to 100.

    abstract：:To define the number of rhinovirus serotypes, cross neutralization tests and characterization studies were completed on 25 candidate prototype rhinoviruses submitted to a third phase of a collaborative program. Based on the results, 11 distinct prototype strains were designated and the numbering system was extended to...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90367-9

    authors： Hamparian VV,Colonno RJ,Cooney MK,Dick EC,Gwaltney JM Jr,Hughes JH,Jordan WS Jr,Kapikian AZ,Mogabgab WJ,Monto A

    更新日期：1987-07-01 00:00:00

  • Structural elements of primary CCR5-using HIV-1 gp120 proteins influencing sensitivity and resistance to the broadly neutralizing monoclonal antibody b12.

    abstract：:Structure-guided approaches to HIV-1 vaccine design depend on knowledge of the presentation of neutralizing epitopes on gp120, such as the epitope for the broadly neutralizing mAb b12. Here, we characterized predicted three-dimensional structures of functionally diverse gp120 proteins in their b12-bound conformation, ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.06.024

    authors： Sterjovski J,Churchill MJ,Ellett A,Wesselingh SL,Ramsland PA,Gorry PR

    更新日期：2012-10-25 00:00:00

  • Glycophorin is the reovirus receptor on human erythrocytes.

    abstract：:Purified glycophorin (predominantly type A) from human erythrocytes was found to effectively inhibit reovirus hemagglutination (HA) in contrast to other glycoproteins such as fetuin or ovalbumin. Glycophorin was also a potent inhibitor of reovirus and protein sigma 1 binding to mouse L fibroblasts. Glycophorin pretrea...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90351-5

    authors： Paul RW,Lee PW

    更新日期：1987-07-01 00:00:00

  • Changes in locations of crossover sites over time in de novo generated RNA recombinants.

    abstract：:Recombinant RNAs generated in plants 3 weeks postinoculation with turnip crinkle virus (TCV) genomic RNA and an associated satellite RNA, sat-RNA D, have a majority of TCV crossover sites in a 24-nucleotide repeat (motif IIIA/IIIB) that forms part of a stable hairpin (Carpenter et al., 1995, J. Mol.Biol. 245, 608-622)...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0465

    authors： Carpenter CD,Simon AE

    更新日期：1996-09-01 00:00:00

  • Characterization of intranuclear capsids made by ts morphogenic mutants of HSV-1.

    abstract：:We have characterized capsids made by seven temperature-sensitive (ts) mutants of HSV-1 previously shown to be defective in viral DNA processing and packaging at the nonpermissive temperature (NPT). The empty capsids isolated from mutant-infected cells at the NPT were devoid of DNA, cosedimented in sucrose with wt B c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90288-7

    authors： Sherman G,Bachenheimer SL

    更新日期：1988-04-01 00:00:00

  • Duck hepatitis B virus covalently closed circular DNA appears to survive hepatocyte mitosis in the growing liver.

    abstract：:Nucleos(t)ide analogues that inhibit hepatitis B virus (HBV) DNA replication are typically used as monotherapy for chronically infected patients. Treatment with a nucleos(t)ide analogue eliminates most HBV DNA replication intermediates and produces a gradual decline in levels of covalently closed circular DNA (cccDNA)...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.08.014

    authors： Reaiche-Miller GY,Thorpe M,Low HC,Qiao Q,Scougall CA,Mason WS,Litwin S,Jilbert AR

    更新日期：2013-11-01 00:00:00

  • Virion formation is required for the long-distance movement of red clover necrotic mosaic virus in movement protein transgenic plants.

    abstract：:The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species. The polycistronic RNA-1 encodes the viral RNA polymerase and capsid protein (CP) and the monocistronic RNA-2 encodes the 35 kDa cell-to-cell movement protein (MP). Nicotiana benthamiana plants transformed with ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1518

    authors： Vaewhongs AA,Lommel SA

    更新日期：1995-10-01 00:00:00

  • Efficient expression of foreign proteins in roots from tobravirus vectors.

    abstract：:Viral vectors were constructed from infectious cDNA clones of each of the three tobraviruses, tobacco rattle virus (TRV), pea early-browning virus (PEBV), and pepper ringspot virus (PepRSV). RNA2 of each of the three viruses was modified to carry an additional coat protein subgenomic promoter and was used to express g...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0098

    authors： MacFarlane SA,Popovich AH

    更新日期：2000-02-01 00:00:00

  • Deep sequencing of Cotesia vestalis bracovirus reveals the complexity of a polydnavirus genome.

    abstract：:Here we completed the whole genome sequence of Cotesia vestalis bracovirus (CvBV) by deep sequencing and compared the genome features of CvBV to those of other polydnaviruses (PDVs). The genome is 540,215 base pairs divided into 35 genomic segments that range from 2.6 to 39.2kb. Comparison of CvBV with other PDVs show...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.03.009

    authors： Chen YF,Gao F,Ye XQ,Wei SJ,Shi M,Zheng HJ,Chen XX

    更新日期：2011-05-25 00:00:00

  • Identification and complete genome analysis of three novel paramyxoviruses, Tuhoko virus 1, 2 and 3, in fruit bats from China.

    abstract：:Among 489 bats of 11 species in China, three novel paramyxoviruses [Tuhokovirus 1, 2 and 3 (ThkPV-1, ThkPV-2 and ThkPV-3)] were discovered in 15 Leschenault's rousettes. Phylogenetically, the three viruses are most closely related to Menangle and Tioman virus. Genome analysis showed that their 3'-leader sequences are ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.03.049

    authors： Lau SK,Woo PC,Wong BH,Wong AY,Tsoi HW,Wang M,Lee P,Xu H,Poon RW,Guo R,Li KS,Chan KH,Zheng BJ,Yuen KY

    更新日期：2010-08-15 00:00:00

  • Phosphorylation state of the cap-binding protein eIF4E during viral infection.

    abstract：:The eukaryotic translation initiation factor eIF4E, the cap-binding protein, seems to play an essential role in the establishment of the host shut-off after viral infection. Infection with adenovirus and influenza virus caused dephosphorylation of eIF4E and an involvement of a viral protein was suggested. In this repo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0143

    authors： Kleijn M,Vrins CL,Voorma HO,Thomas AA

    更新日期：1996-03-15 00:00:00

  • Nucleocytoplasmic shuttling of the human parainfluenza virus type 2 phosphoprotein.

    abstract：:Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cy...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.12.005

    authors： Ohtsuka J,Matsumoto Y,Ohta K,Fukumura M,Tsurudome M,Nosaka T,Nishio M

    更新日期：2019-02-01 00:00:00

  • Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection.

    abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of natura...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.07.009

    authors： Goldberg TL,Lowe JF,Milburn SM,Firkins LD

    更新日期：2003-12-20 00:00:00

  • Sequence analysis of the phosphoprotein (P) genes of human parainfluenza type 4A and 4B viruses and RNA editing at transcript of the P genes: the number of G residues added is imprecise.

    abstract：:We cloned and sequenced the cDNAs against genomic RNAs and mRNAs for phosphoproteins (Ps) of human parainfluenza virus types 4A (PIV-4A) and 4B (PIV-4B). The PIV-4A and -4B P genes were 1535 nucleotides including poly(A) tract and were found to have two small open reading frames, neither of which was apparently large ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90413-l

    authors： Kondo K,Bando H,Tsurudome M,Kawano M,Nishio M,Ito Y

    更新日期：1990-09-01 00:00:00

  • Interferon-λ4 is a cell-autonomous type III interferon associated with pre-treatment hepatitis C virus burden.

    abstract：:Genetic variants surrounding the interferon-λ3 (IFNL3) gene are strongly associated with clearance of hepatitis C virus (HCV). A variant (rs368234815 TT/ΔG) upstream of IFNL3 was recently implicated to control expression of a novel gene termed IFNL4. We conducted genetic analysis of rs368234815 in a chronic HCV patien...

    journal_title：Virology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.virol.2014.12.020

    authors： Lu YF,Goldstein DB,Urban TJ,Bradrick SS

    更新日期：2015-02-01 00:00:00

  • Inhibition of the epidermal growth factor receptor by erlotinib prevents immortalization of human cervical cells by Human Papillomavirus type 16.

    abstract：:The Human Papillomavirus type-16 (HPV-16) E6 and E7 oncogenes are selectively retained and expressed in cervical carcinomas, and expression of E6 and E7 is sufficient to immortalize human cervical epithelial cells. Expression of the epidermal growth factor receptor (EGFR) is often increased in cervical dysplasia and c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.09.014

    authors： Woodworth CD,Diefendorf LP,Jette DF,Mohammed A,Moses MA,Searleman SA,Stevens DA,Wilton KM,Mondal S

    更新日期：2011-12-05 00:00:00

  • Herpes simplex virus immediate early gene expression in the absence of transinduction by Vmw65 varies during the cell cycle.

    abstract：:The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90695-l

    authors： Daksis JI,Preston CM

    更新日期：1992-07-01 00:00:00

  • Infectious Bursal Disease Virus non-structural protein VP5 is not a transmembrane protein.

    abstract：:Infectious Bursal Disease Virus (IBDV) causes a highly relevant poultry disease that affects young chickens causing, among other effects, immunosuppression. IBDV is a bi-segmented double stranded RNA virus. The smaller ORF of larger RNA segment encodes VP5, a 17-kDa non-structural protein. Although it is an important ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.05.003

    authors： Carballeda JM,Maroniche G,Lucero MS,Richetta M,Gómez E,Chimeno Zoth S,Berinstein A

    更新日期：2015-09-01 00:00:00

  • Antigenic variants of bovine leukemia virus (BLV) are defined by amino acid substitutions in the NH2 part of the envelope glycoprotein gp51.

    abstract：:Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90037-8

    authors： Portetelle D,Couez D,Bruck C,Kettmann R,Mammerickx M,Van der Maaten M,Brasseur R,Burny A

    更新日期：1989-03-01 00:00:00

  • Rapid establishment of stable retroviral packaging cells and recombinant susceptible target cell lines employing novel transposon vectors derived from Sleeping Beauty.

    abstract：:Viral vector particles derived from murine leukemia virus (MLV) mediate highly efficient stable gene transfer used in gene therapeutic approaches and in the generation of transgenic cell lines. However, the establishment of stable viral packaging cells (VPCs) is a time-consuming challenge. To overcome this limitation,...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.02.014

    authors： Berg K,Schäfer VN,Bartnicki N,Eggenschwiler R,Cantz T,Stitz J

    更新日期：2019-05-01 00:00:00

  • Protective properties of non-nucleoside reverse transcriptase inhibitor (MC1220) incorporated into liposome against intravaginal challenge of Rhesus macaques with RT-SHIV.

    abstract：:In the absence of an effective vaccine against HIV, it is urgent to develop an effective alternative such as a microbicide. Single and repeated applications of MC1220 microbicide were evaluated in macaques. First, animals were given a single application of 0.5% or 1.5% MC1220-containing liposomal gel. A second group w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.06.008

    authors： Caron M,Besson G,Etenna SL,Mintsa-Ndong A,Mourtas S,Radaelli A,Morghen Cde G,Loddo R,La Colla P,Antimisiaris SG,Kazanji M

    更新日期：2010-09-15 00:00:00

  • Transforming activity of the E6 gene of HPV-11gt in NIH 3T3 and REF 52 cells: correlation with the level of E6 transcription.

    abstract：:Among human papillomavirus (HPV) types, clinical association with benign vs malignant lesions correlates with the ability of the corresponding oncogenes to transform cells in vitro. However, even though HPV-11 is considered a low-risk type, we have reported previously that the E5a oncogene of HPV-11gt is capable of tr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0279

    authors： Trujillo JM,Mounts P

    更新日期：1996-06-01 00:00:00